HPLC法测定木蝴蝶提取物中木蝴蝶苷A的含量

目的：建立木蝴蝶提取物中木蝴蝶苷A的HPLC测定方法。方法：色谱柱：DIONEX—ACCLAIM．120 C18（250mm×4．6mm,5μm）;流动相：甲醇（A）-0．02％磷酸水溶液（B）;流速：0．5ml·min^-1;柱温：35℃;检测波长：280nm.结果：木蝴蝶提取物的线性范围为1．005～6．030μg,r=0．9999。平均加样回收率为95．30％,RSD为0．59％（n=6）。结论：该方法准确,重复性好．专属性高,适用于木蝴蝶提取物的质量控制。     

木蝴蝶提取工艺的研究

目的:通过对木蝴蝶的提取工艺进行分析研究,确定其最佳提取工艺。方法:采用正交试验法进行优选,以木蝴蝶苷B含量作为评价指标。结果:最佳工艺为:微沸提取2次,第1次加7倍水,第2次加5倍水,每次0.5h,醇沉浓度为65%。结论:该提取工艺稳定可靠,得到的木蝴蝶苷B含量高。     

HPLC法测定中药木蝴蝶中黄芩苷和白杨素

目的：建立HPLC测定中药木蝴蝶干燥种子中的黄芩苷和白杨素的方法。方法使用岛津2010 AHT型高效液相色谱系统,Waters Xtera C18色谱柱（150 mm ×4．6 mm ,5μm）,以乙腈-0．5 mL · L-1甲酸水溶液（50∶50）为流动相,流速1．0 mL · min-1,进样量10μL ,双波长检测,检测波长分别为黄芩苷280 nm ,白杨素275 nm ,柱温为30℃。结果在该色谱条件下,黄芩苷和白杨素分离良好,可在较短时间内对2个化合物进行定量分析。结论该方法快速、稳定,能够用于木蝴蝶的质量控制。     

高效液相色谱法测定木蝴蝶中黄芩苷含量

用高效液相色谱法测定木蝴碟中黄芩苷的含量.采用Kromasil C18柱分离黄芩苷,甲醇-水-冰醋酸(50:50:1)为流动相,检测波长为280nm.线性范围0.4～2.0μg(r=0.99999);平均回收率为97.77%,RSD=0.70%(n=5).本方法简便、快速,可用于木蝴蝶中黄芩苷的含量测定.     

川芎药材的高效液相色谱质量控制方法研究

以川芎药材中主要药效成分为指标物质，建立一个可以用于评价和控制川芎药材质量的HPLC方法。方法：川芎总提物经溶剂萃取样品前处理，采用反相HPLC分析。色谱柱为Agilent Extend-C18流动相梯度洗脱程序为在40min内甲醇和1％乙酸水溶液从体积比35：65线性变化为80：20，流速为0．8mL．min^-1；柱温为室温；紫外检测波长为290nm。结果：6种川芎主要药效成分在35min内得到完全分离。结论：方法简单、重现性好、稳定、可靠，可用于川芎药材的质量控制。     

HPLC法测定芪贞慢咽宁颗粒中木蝴蝶苷B

目的建立HPLC法测定芪贞慢咽宁颗粒中木蝴蝶苷B。方法采用Kromasil 100-5 C18色谱柱(250 mm×4.6 mm,5μm),流动相甲醇–0.1%磷酸(38∶62),检测波长276 nm,体积流量1.0 m L/min,柱温30℃,进样量5μL。结果木蝴蝶苷B在0.064 6~1.292 0μg与峰面积线性关系良好。木蝴蝶苷B平均回收率为100.67%,RSD值为1.12%。结论本法测定快速、结果准确、重复性好、专属性强,可用于测定芪贞慢咽宁颗粒中木蝴蝶苷B。     

复方银杏叶口服液的质量控制方法研究

目的建立复方银杏叶口服液的质量控制方法。方法填充剂:以十八烷基硅烷键合硅胶为填充剂;流动相:甲醇-0.4%磷酸(50∶50);检测波长:360nm;流速:1.0mL/min。供试品经盐酸水解法前处理,高效液相色谱法测定银杏总黄酮醇苷。结果槲皮素(Q)、山柰素(K)、异鼠李素(I)进样量分别在0.06~0.60、0.06~0.60、0.04~0.40μg范围内与峰面积积分值呈良好的线性关系,Y=43.75X-34.30、r=0.9998;Y=43.96X-18.66、r=0.9998;Y=41.67X-35.72、r=0.9999;峰面积RSD(n=5)分别为1.54%、1.85%、1.63%;平均回收率分别为99.19%、99.38%、98.90%。结论本方法重现性好,敏感度高,可以作为复方银杏叶口服液的经验性质量控制方法。     

复方银杏叶胶囊质量控制方法研究

目的建立复方银杏叶胶囊的质量控制方法。方法供试品经盐酸水解法前处理，采用高效液相色谱法测定银杏总黄酮醇苷含量。结果槲皮素、山柰素、异鼠李素色谱峰分离良好（R〉1．5），供试品其他组分对其均无干扰，线性回归方程分别为Y1=43．75X1-34．30（r1=0．9998），Y2=43．96X2—18．66（r2=0．9998），Y3=41．67X3-35．72（r3=0．9999）；峰面积RSD分别为1．54％，1．85％，1．63％（n=5），平均回收率分别为99．21％，99．38％，99．24％。结论该方法重现性好，灵敏度高，可以作为复方银杏叶胶囊的质量控制方法。     

胃舒宁冲剂质量控制研究

目的：探讨胃舒宁冲剂的质量。方法：采用薄层色谱法鉴别方中甘草、延胡索；用高效液相色谱法测定芍药苷的含量。结果：定性定量方法简便、专属性强、准确、重现性好。结论：所建立的方法可用于胃舒宁冲剂的质量控制。     

一叶萩药材质量控制方法研究

目的 建立一叶萩药材的质量控制方法。方法 参照2020年版《中国药典》（四部）方法对一叶萩药材进行显微鉴别和薄层色谱（TLC）鉴别,并测定药材中的水分、总灰分及酸不溶性灰分、醇溶性浸出物;采用高效液相色谱（HPLC）法测定药材中一叶萩碱的含量。结果 一叶萩药材的粉末呈灰绿色,气孔、花粉粒、草酸钙簇晶、导管等显微特征明显。TLC鉴别结果显示,16批药材样品的供试品色谱中,在与一叶萩碱、芦丁、槲皮素对照品及一叶萩对照药材色谱相应的位置上分别显相同颜色的斑点。16批药材样品的水分、总灰分、酸不溶性灰分、醇溶性浸出物的平均质量分数分别为9.26%、6.96%、1.17%、28.89%。一叶萩碱进样量在0.052 4～0.524 0μg范围内与峰面积成良好的线性关系（R2=0.999 8）;精密度、重复性、稳定性（24 h）试验的RSD均小于3%(n为6或7);平均加样回收率为97.47%,RSD为1.63%(n=6)。16批药材样品中的一叶萩碱含量为1.003～6.872 mg/g。结论 建立了一叶萩药材的质量控制方法,并初步拟定该药材中水分、总灰分及酸不溶性灰分分别不得过12.0%、9.0%...     

Immunomodulatory Activity of n-Butanol Extract of Oroxylum indicum

The present study evaluated the immunomodulatory activity and mechanism of action of the n-butanol extract [100 mg/kg body weight (b.w.), per oral (p.o.)] of Oroxylum indicum Vent. (Bignoniaceae) root bark against different experimental animal models, i.e., immune response to sheep red blood cells in stress-induced immunosuppression, carbon clearance assay and neutrophil adhesion test. In the immune response to sheep red blood cell model, the n-butanol extract of Oroxylum indicum treatment group showed a significant rise (P < 0.05) in circulating antibody-titer as compared to the stress-induced control group. This observation suggests that the drug treatment caused potentiation of humoral antibody immune response to antigen. Also, it significantly decreases the rise in malondialdehyde content along with an increase (p < 0.05) in superoxide dismutase, catalase and reduced glutathione levels as compared to the stress-induced control group and, thus, exhibits significant antioxidant potential. Pretreatment with the n-butanol extract of Oroxylum indicum showed a significant rise (P < 0.05) in phagocytic index indicating phagocytic activity. In the neutrophil adhesion model, a significant increase (P < 0.05) in percentage neutrophil adhesion was observed in the n-butanol extract treatment group as compared to the control group. From these findings, the n-butanol extract of Oroxylum indicum possesses immunomodulatory activity by enhancing specific immune response (humoral immunity) and non-specific immune response (phagocytosis) of the body as well as exhibiting antioxidant potential. Our results suggest that the n-butanol extract of Oroxylum indicum root bark possesses a significant immunostimulant activity.     

Antiulcer Activity of the Root Bark of Oroxylum indicum. Against Experimental Gastric Ulcers

Abstract

The current study was undertaken to investigate the effect of the root bark of Oroxylum indicum. Vent. (Bignoniaceae) against experimental gastric ulcers. The 50% alcohol extract of root bark of Oroxylum indicum. and its different fractions, viz., petroleum ether, chloroform, ethyl acetate and n.-butanol, were studied (p.o.) against ethanol-induced gastric mucosal damage. The alcohol extract (300 mg/kg) and its different fractions (100 and 300 mg/kg) showed significant reduction in gastric ulceration. Out of all these fractions, the petroleum ether (96%) and n.-butanol (99%) fractions showed maximum inhibition of gastric lesions against ethanol-induced gastric mucosal damage. The results were comparable with omeprazole (reference standard). In the ethanol-induced gastric ulcer model, treatment with both the active fractions and omeprazole showed significant antioxidant activity as evident from the reduction in the extent of lipid peroxidation that was measured in terms of malondialdehyde (MDA), along with significant rise in the superoxide dismutase (SOD), catalase (CAT), and reduced glutathione levels (GSH), when compared with the control group. In 6-h pylorus-ligated animals, active fractions of drug at 100 mg/kg showed significant reduction in the ulcer index. Furthermore, in the pylorus-ligation model, significant reduction (p < 0.05) was observed in total acidity, total acid output, pepsin activity, and pepsin output, along with a significant rise in the total carbohydrate to protein ratio (reflecting mucin activity) when compared with the control group. TLC studies revealed the presence of baicalein in the petroleum ether and hydrosylate in n.-butanol fraction. Fingerprinting of both the active fractions was developed by performing HPLC analysis. Baicalein was found to be a major flavonoid present both in petroleum ether and n.-butanol hydrosylate. The mechanism of its antiulcer activity could be attributed to a decrease in gastric acid secretory and antioxidant activities leading to gastric cytoprotection. This activity could be linked to the presence of baicalein in the root bark of the plant.     

木蝴蝶含片的研制

摘 要 目的： 制备木蝴蝶含片。方法: 以木蝴蝶提取物为主药,采用湿法制粒压片法,在单因素考察基础上,以含片外观、口感、崩解时限、硬度为指标,通过正交设计试验对处方进行优化筛选,确定含片的制备处方。以平均重量差异和平均崩解时间为指标,对按照优化的处方工艺制备的3批含片进行质量检查。结果: 优选的处方为：甘露醇50%,甜味剂为蔗糖-甜菊糖苷（100∶1）5%,清凉剂为天然薄荷油5%,粘合剂为聚维酮-K30（PVP-K30）-85%乙醇（6∶10）35%。以筛选的处方工艺制备的3批含片平均重量差异为 (1.83±0.29) %,平均崩解时间为（14.7±0.6）min,均符合药典质控要求。结论: 木蝴蝶含片制备工艺稳定,重复性良好。     

Phytochemical Screening and Evaluation of Analgesic Activity of Oroxylum indicum

We aimed to study phytochemical screening and analgesic activity of ethanol extract of Oroxylum indicum. The dried powder of the barks of the plant was extracted with 95% ethanol and was subjected to various phytochemical tests to ascertain the principle constituents contained in the extract. The result revealed the presence of alkaloids, flavonoids, tannins, glycosides in the ethanol extract of Oroxylum indicum. The extract was screened for analgesic activity by using hot plate, acetic acid-induced writhing and formalin test. The ethanol extract of the plant at two different doses (250 and 500 mg/kg) showed significant (P<0.05) analgesic effect in all test methods (hot plate, acetic acid-induced writhing and formalin). The analgesic activity was compared with a standard drug (ketorolac at 10 mg/kg). Based on the present findings and previous literature review it can be concluded that flavonoids and tannins might be responsible for the analgesic activity. We suggest that ethanol extract of Oroxylum indicum might have potential chemical constituents that could be used in the future for the development of novel analgesic agent.     

In vitro Antioxidant Potential of Different Parts of Oroxylum indicum: A Comparative Study

The present study evaluated the in vitro antioxidant potential of different parts of Oroxylum indicum. 2,2-diphelyl 1-picrylhydrazyl (DPPH), nitric oxide, superoxide anion and hydroxyl radical scavenging potential and reductive ability assay of methanol extract of different parts i.e. root, root bark, stem, stem bark, leaves and fruits were performed. Leaves and bark extracts exhibits highest free radical scavenging activity than bark, stem and fruit extract. Leaves extract showed maximum reductive ability and found to contain maximum amount of polyphenolic compounds. The highest free radical activity may be due to presence of polyphenolic compounds.     

前列丹颗粒的质量控制方法研究

摘 要 目的： 建立前列丹颗粒的质量控制方法。方法: 采用薄层色谱法薄层对方中半枝莲、黄芪、炙甘草、补骨脂、姜黄进行定性鉴别;以高效液相色谱法对君药半枝莲中野黄芩苷进行含量测定。结果: 薄层斑点清晰,分离良好,阴性无干扰;野黄芩苷在3.96~79.20 μg·mL^-1范围内呈良好线性关系（r=0.999 9）,平均加样回收率为99.34%（RSD=1.25%,n=9）。结论:本研究建立的质量控制方法准确、可靠、稳定、专属性强,可有效用于前列丹颗粒的质量控制。     

抗痨胶囊质量控制研究

目的:建立抗痨胶囊质量控制方法。方法:采用TLC法鉴别胶囊中异海松酸,HPLC法测定异海松酸含量,色谱条件Kromasil C_(18)柱(200 mm×4.6mm,5μm)甲醇-水-冰醋酸(60:37:3)为流动相,检测波长231 nm,流速1ml·min~(-1)。结果:TLC法能检出异海松酸,异海松酸的线性范围为1.25～10.00μg(r=1.000 0);加样回收率98.1%,RSD=2.3%(n=5)。结论:本法可作为抗痨胶囊质量控制方法。     

虎耳草颗粒的质量控制方法

目的建立虎耳草颗粒的质量控制方法。方法色谱柱为hypersil ODS(250 mm×4.0 mm、5μm),流动相为甲醇-水(20∶80),流速为1.0 mL/min,检测波长275 nm,柱温30℃。结果岩白菜素的进样量在0.0202⁰.202μg之间与峰面积呈良好线性关系(R2=0.9998),平均回收率为100.33%,RSD=1.92%。结论本方法灵敏、准确、重现性好,可用于虎耳草颗粒的质量控制。