Clinical characteristics of parainfluenza virus infection in hospitalized children.

Severity of illness and clinical characteristics of parainfluenza virus (PIV) infection were evaluated in 81 hospitalized children over a 4 year period. Fifty three patients were previously healthy and 28 had preexisting pulmonary abnormalities associated with bronchopulmonary dysplasia (BPD), congenital heart disease (CHD), asthma, or prematurity. When compared with formerly healthy children, the patients with preexisting pulmonary abnormalities were more likely to develop lower than upper respiratory tract illness (P less than 0.0001). In the lower respiratory tract infection group, patients with preexisting pulmonary abnormalities were sicker (P = 0.047), were hospitalized longer (P = 0.016), required more supplemental oxygen (P = 0.004), and were older (8.8 vs. 5.1 months) than previously healthy patients. Nosocomial infection occurred only in BPD patients. All these patients developed pneumonia. They were sicker (P = 0.0018), requiring more therapy (P = 0.0038) than other patients with preexisting pulmonary abnormalities and lower respiratory tract disease. Patients with BPD should be placed in protective isolation during PIV epidemics.     

Intensive care course and outcome of patients infected with respiratory syncytial virus.

PICU admissions of 97 children positive for respiratory syncytial virus on fluorescent antibody screening were reviewed; 68% of 44 patients without history of preceding disease (Group I) and 79% of 53 patients with preceding pulmonary, cardiac, or other disease (Group II) required ventilation. In Group I ventilated children weighted significantly less (P = 0.001) and were of lower chronological (P = 0.02) and post-conceptional ages (P = 0.02) than those not ventilated. Eighteen infants ventilated for apnea weighted significantly less (P = 0.003), were more often born at less than or equal to 37 weeks gestation (P = 0.001) and were at lower post-conceptional age than 11 infants ventilated for progressive respiratory deterioration. There was no significant difference in mean weight, chronological age, post-conceptional age, CO2, or pH between 12 admissions with BPD who required ventilation for RSV infection and 5 who did not require ventilation. Ribavirin administration to five ventilated patients with BPD did not significantly alter the duration of intubation of PICU stay. Six patients with cardiac disease required longer periods of ventilation than others (Group I, P = 0.001; all others in Group II, P = 0.04). No deaths occurred in Group I, while 6 of 53 (11%) patients in Group II died. In this series immuno compromise placed patients at greatest risk of dying. Mechanical ventilation can be safely managed in previously healthy, RSV infected infants and should be initiated before significant cardiorespiratory compromise arises.     

Early ribavarin treatment of bronchiolitis: effect on long-term respiratory morbidity

BACKGROUND: The mortality rate from respiratory syncytial virus (RSV) bronchiolitis has significantly reduced over the last decade. A major concern now is the long-term respiratory morbidity following RSV bronchiolitis. METHODS: In this prospective study, we randomly assigned 49 previously healthy infants with severe RSV bronchiolitis, early in the course of illness (< 5 days duration), to receive either conservative treatment (n = 21) or additional ribavirin treatment (n = 24). Both groups were closely matched for age and clinical characteristics. RESULTS: During a prospective, closely monitored, 1-year follow-up period, the group treated with ribavirin had significantly fewer episodes (2.7 +/- 2.3 episodes vs 6.4 +/- 4.2 episodes per patient per year) and reduced severity of reactive airway disease (0.08 episodes vs 1.09 episodes of moderate-to-severe illness per patient per year) and respiratory illness-related hospitalization (25 hospital days vs 90 hospital days per 100 patients per year). CONCLUSIONS: Early ribavirin treatment of RSV bronchiolitis in previously healthy infants resulted in reduction of incidence and severity of reactive airway disease as well as respiratory illness-related hospitalization.     

老年不稳定性心绞痛择期冠状动脉介入治疗与强化药物治疗的对比研究

目的通过对老年不稳定性心绞痛的冠心病患者经择期PCI和单纯优化药物治疗住院期间及治疗后近期心血管事件比较,探讨老年不稳定性心绞痛的治疗选择。方法随机选择老年不稳定性心绞痛患者268例,根据是否行介入治疗分为:介入组134例(发病后3～7d接受择期PCI)和药物组134例(未行PCI术,单纯优化药物治疗),分别比较住院10d、出院30、90d后2组主要不良心脏事件及左心室功能。结果出院30、90d随访2组主要不良心脏事件,差异无统计学意义(P>0.05)。介入组再发心绞痛及左心室功能较药物组明显改善(P<0.05),介入组住院期间发生出血事件明显高于药物组(P<0.05)。结论对于老年不稳定性心绞痛行择期PCI有利于左心室功能的改善和抑制左心室重构,择期PCI治疗老年不稳定性心绞痛患者是可行的。     

综合护理干预对老年冠心病患者焦虑抑郁情绪的临床效果研究

目的 探讨综合护理对老年冠心病患者焦虑情绪改善情况。 方法  将2012~2014年在本院就诊的90例老年冠心病患者随机分成实验组与对照组,每组45例。对照组采取常规护理及治疗,实验组在常规护理的基础上,进行综合护理干预。采用问卷调查及出院后随访的方式比较两组患者在护理前后对疾病认知程度、生活方式及焦虑情绪的差异。结果 干预后,实验组患者在对疾病的认识程度、生活习惯、健康状况及心理状态上均有明显改善,患者在吸烟(>5支/d)、饮酒(乙醇量>30g/d)、高脂、高盐摄入、缺乏运动上分别下降了85.7%、91.6%、87.5%、83.3%、88.6%;而对照组仅下降26.4%、56.7%、64.1%、43.9%、63.8%;实验组患者无焦虑及轻度焦虑共计40例(88.8%);对照组为33例（73.3%）。差异均有统计学意义（P < 0.05）。结论 综合护理干预可促进老年冠心病患者建立正确的生活方式,缓解焦虑等负面情绪,从而提高患者生活质量及健康状况。     

运动疗法对冠心病患者介入治疗后运动能力的影响

目的：观察运动疗法对冠心病经皮冠状动脉介入治疗（PCI）后患者运动能力的影响。方法：确诊为冠心病并成功进行首次PCI的患者86例，被随机分为运动疗法组（41例）和对照组（45例），两组患者除进行PCI治疗外，均采用常规疗法。运动疗法组于PCI术后开始进行不同阶段、不同运动强度的运动康复训练。分别于PCI术后第10d、3个月后，行心电图活动平板运动试验。观察运动疗法对冠心病患者PCI治疗后运动能力的影响。结果：经运动疗法治疗3个月后，运动疗法组患者的总运动时间，最大运动负荷量，最大代谢当量均有显著提高（P〈O．05～〈O．01），而对照组则无明显变化。结论：运动疗法有助于冠心病介入治疗后患者体能的提高。     

Prospective Population-based Study of RSV-related Intermediate Care and Intensive Care Unit Admissions in Switzerland over a 4-Year Period (2001–2005)

Abstract Objectives:   Respiratory syncytial virus (RSV) infections are a leading cause of hospital admissions in small children. A substantial proportion of these patients require medical and nursing care, which can only be provided in intermediate (IMC) or intensive care units (ICU). This article reports on all children aged < 3 years who required admission to IMC and/or ICU between October 1, 2001 and September 30, 2005 in Switzerland. Patients and Methods:   We prospectively collected data on all children aged < 3 years who were admitted to an IMC or ICU for an RSV-related illness. Using a detailed questionnaire, we collected information on risk factors, therapy requirements, length of stay in the IMC/ICU and hospital, and outcome. Results:   Of the 577 cases reported during the study period, 90 were excluded because the patients did not fulfill the inclusion criteria; data were incomplete in another 25 cases (5%). Therefore, a total of 462 verified cases were eligible for analysis. At the time of hospital admission, only 31 patients (11%) were older than 12 months. Since RSV infection was not the main reason for IMC/ICU admission in 52% of these patients, we chose to exclude this subgroup from further analyses. Among the 431 infants aged < 12 months, the majority (77%) were former near term or full term (NT/FT) infants with a gestational age ≥ 35 weeks without additional risk factors who were hospitalized at a median age of 1.5 months. Gestational age (GA) < 32 weeks, moderate to severe bronchopulmonary dysplasia (BPD), and congenital heart disease (CHD) were all associated with a significant risk increase for IMC/ICU admission (relative risk 14, 56, and 10, for GA ≤ 32 weeks, BPD, and CHD, respectively). Compared with NT/FT infants, high-risk infants were hospitalized at an older age (except for infants with CHD), required more invasive and longer respiratory support, and had longer stays in the IMC/ICU and hospital. Conclusions:   In Switzerland, RSV infections lead to the IMC/ICU admission of approximately 1%–2% of each annual birth cohort. Although prematurity, BPD, and CHD are significant risk factors, non-pharmacological preventive strategies should not be restricted to these high-risk patients but also target young NT/FT infants since they constitute 77% of infants requiring IMC/ICU admission.     

冠心病患者血小板活化、凝血、纤溶指标检测的临床应用

目的：探讨冠心病（CHD）者血中血小板活化、凝血、纤溶指标变化及其意义。方法：对145例CHD患者和40例正常对照者血小板α-颗粒膜糖蛋白-140（GMP-140）、组织因子（TF）、纤维蛋白原（Fib）及D-二聚体水平进行测定、分析。结果：急性心肌梗死组、不稳定心绞痛组以及冠状动脉存在2支及2支以上病变患者的GMP-140、TF、Fib及D-二聚体水平均显著高于正常对照组的（P〈0.05）;稳定心绞痛组TF和Fib水平均显著高于正常对照组的（P〈0.05）。结论：GMP-140、TF、Fib及D-二聚体水平与CHD病情相关。检测GMP-140、TF、Fib及D-二聚体水平,对CHD的诊断和病情监测有一定价值。     

Risk factors for recurrent wheezing following acute bronchiolitis: a 12-month follow-up

The objective of this study was to identify wheezing recurrences and related risk factors in two groups of infants with bronchiolitis: respiratory syncytial virus (RSV)+ and RSV- as determined by RSV enzyme immunoassay. A 1-year prospective cohort study was conducted with infants younger than 2 years old. Follow-up was made monthly, by a clinical visit and/or by telephone, checking the number of wheezing episodes per month and possible related risk factors. There were 96 subjects enrolled, of whom 77 reached complete follow-up: 36 were RSV+ (46.8%), and 41 were RSV- (53.2%). In the RSV+ group, there were 17 males (47%), vs. RSV- with 30 males (73%) (P < 0.05); 22 RSV+ (61%) were admitted to hospital, vs.14 RSV- (34%) (P < 0.05). Mean age was not significantly different in both groups. The mean number of recurrences was 3.36 episodes/infant/year in the RSV+ and 2.34 in the RSV- group (P = 0.06). Crude relative risk (RR) for a new recurrence of an obstructive episode was 1.33 (95% CI, 0.99-1.79). After adjustment for several potential confounders, the RR was 1.41 (95% CI, 1.03-1.93). Hospitalization stay was longer in the RSV+ than the RSV- group (P < 0.05). In the RSV+ group, patients who had been hospitalized showed more recurrences (4.18) than those with outpatient treatment (2.07) (P < 0.05); this difference did not exist in the RSV- group. The related risk factors for recurrent wheeze in the RSV- group were male gender, number of siblings, and daycare attendance (P < 0.05). In the RSV+ group, the risk of recurrent wheeze was only increased by admission to hospital during the acute bronchiolitis episode (P < 0.05). We speculate that there may be a higher rate of increased airway reactivity and/or preexisting diminished lung function in RSV+ infants requiring hospitalization for their initial illness. In conclusion, RSV-proven bronchiolitis, particularly in those infants who are hospitalized, is associated with a higher recurrence of wheezing episodes in the subsequent 12 months. Other factors appear to account for recurrent wheeze in the RSV- group.     

Serum lipoproteins, sex hormones and sex hormone binding globulin in middle-aged men of different physical fitness and risk of coronary heart disease

Serum concentrations of lipoproteins, apolipoprotein A-I (Apo A-I), androgens, including biologically active free testosterone (free T), and sex hormone binding globulin (SHBG) and their associations were studied in 3 groups of men of different physical fitness and risk of CHD, consisting of male CHD patients, joggers and healthy controls. Of the 3 study groups, men with angiographically assessed CHD had the lowest HDL-C (P less than 0.002) and highest LDL-C and triglyceride (TG) levels (P = 0.05 and P less than 0.001) and lower 5 alpha-dihydrotestosterone (5 alpha-DHT) levels than joggers (P less than 0.02). Joggers had the highest serum high density lipoprotein cholesterol (HDL-C), Apo A-I and SHBG levels and lowest serum low density lipoprotein cholesterol (LDL-C) compared to the other groups (P less than 0.01). In correlation analysis 5 alpha-DHT was the most significant positive determinant of HDL-C and Apo A-I levels in CHD patients (r = 0.56 and r = 0.55, respectively, P less than 0.05). Moreover, SHBG was significantly positively correlated to both HDL-C and Apo A-I levels in patients, in the whole study group and in healthy men separately (r = 0.37-0.52, P less than 0.01). These significant correlations were also confirmed when age variation and differences in body mass index and smoking were controlled in multivariate analysis and in addition, in multivariate analysis both serum free and total testosterone were inversely related to serum triglyceride (TG) levels.(ABSTRACT TRUNCATED AT 250 WORDS)     

胸痹心痛的临床证型与部分冠心病危险因素的对比研究

目的明确冠心病的常见危险因素对胸痹心痛的证型影响,以寻找胸痹心痛的辨证客观化的宏观指标.方法对375例胸痹心痛病人进行辨证分型和冠状动脉造影检查,对比分析冠心病的不同危险因素对证型的影响.结果女性阴虚证和气滞证的比例高于男性,男性血瘀证(52.1%)显著高于女性(31.5%).男性冠心病组的血瘀证显著高于男性非冠心病组和女性非冠心痛组(P＜0.05).冠状动脉狭窄组的女性的阴虚证和气滞证比例显著高于男性;女性的冠心病组和冠状动脉正常组的阴虚证显著高于男性正常组.虚证组的体重指数、发病年龄、胸痹心痛病程均分别低于虚实夹杂证(P＜0.05).内向组的气滞证显著高于性情平和组和性情急躁组,而痰浊证则显著低于后两组(P＜0.05).性情急躁组的气滞证显著高于性情平和组(P＜0.05).非吸烟者的阴虚证显著高于吸烟者(P＜0.05),吸烟者的血瘀证和痰浊证高于非吸烟者.饮酒对胸痹心痛的证型分布无显著性影响.肉食嗜好组的痰浊证显著高于非肉食嗜好组(P＜0.05),肉食嗜好者的冠心病组和的痰浊证显著高于冠脉正常组(Fat1c0)和无肉食嗜好的冠心病组(P＜0.05);早发冠心病家族史的证型分布无特殊性.结论性别、体重指数、患病年龄、胸痹心痛病程、性情、吸烟、肉食嗜好的临床证型各有特点,而饮酒嗜好和早发冠心病家族史的不同其证型无特殊.     

阿托伐他汀对冠心病患者血中趋化因子水平的影响

目的观察冠心病患者血浆中趋化因子的变化,并进一步探讨阿托伐汀对趋化因子的影响。方法健康对照组25例,血脂正常的冠心病70例,随机将冠心病患者分为常规治疗组和阿托伐汀组,检测血浆中单核细胞趋化蛋白-1(monocyte chemoattract protein-1,MCP-1)、T细胞表达和分泌活化蛋白(regulated upon activation normal T cell expressed and secreted,RANTES)及白介素-8(interleukin-8,IL-8)水平。结果与健康对照组相比,冠心病患者血浆中MCP-1、RANTES、IL-8水平明显升高(P<0.01)。常规治疗组和阿托伐汀组治疗4周后MCP-1、RANTES、IL-8水平均有下降(P<0.05,P<0.01),阿托伐汀治疗组较常规治疗组降趋化因子的作用更明显(P<0.05)。结论阿托伐汀降低冠心病患者血浆中趋化因子水平,可能起到稳定粥样斑块的作用。     

冠心病患者介入治疗前后循环内皮祖细胞数量的变化

目的 观察并比较正常人与不同类型冠心病患者外周血中内皮祖细胞(EPCs)数量的差异,以及不同类型冠心病患者在经皮冠状动脉介入治疗(PCI)前后外周血中EPCs的数量变化.方法 将患者分成4组:稳定性心绞痛组、不稳定性心绞痛组、急性ST段抬高型心肌梗死组及正常对照组.利用流式细胞术双色分析法检测对照组以及3组不同类型冠心病患者在PCI术前、术后即刻、术后24 h EPCs占外周血有核细胞的百分比,其中EPCs以CD133~+/VEGFR-2~+双标记阳性确定.结果 PCI术前,外周血中EPCs数量在稳定性心绞痛组为(0.043±0.043)%、不稳定性心绞痛组为(0.014±0.018)%、急性ST段抬高型心肌梗死组为(0.040±0.036)%,均明显低于正常对照组[(0.111%±0.078)%](均P＜0.01),不稳定性心绞痛组外周血中EPCs的数量明显低于稳定性心绞痛组(P＜0.05).不稳定性心绞痛组外周血中EPCs的数量在PCI术后24 h较术前明显增加[(0.054±0.045)%比(0.014±0.018)%,P＜0.01].稳定性心绞痛组及急性ST段抬高型心肌梗死组PCI术前、术后EPCs的数量差异无统计学意义.结论 外周血中EPCs数量的变化可能与冠心病的病变程度有关.PCI对外周血EPCs数量的影响可能与冠心病类型有关.     

2型糖尿病合并冠心病患者心率变导与QT离散度的关系

目的　探讨 2型糖尿病 (T2DM )合并冠心病 (CHD)的心率变异 (HRV)与QT离散度 (QTd)的关系。方法　选择 2 0 0 1- 0 2～ 2 0 0 4 - 0 2东莞市人民医院 2型糖尿病 33例 (B组 )、2型糖尿病合并冠心病 30例 (A组 )患者 ,同时选择正常人 2 1例做对照 (C组 ) ,分别做 2 4h动态心电图 ,经电脑系统分析得出HRV时域和频域分析参数 ,做常规 12导联同步心电图 ,测定QT间期及QTd。结果　 (1)A组的HRV各项参数均低于B组和C组 (P<0 0 1) ,QTd均大于B组和C组 (P <0 0 5 ,P <0 0 1) ;B组的HRV各项参数亦均低于C组 (P <0 0 5 ) ,QTd亦均大于C组 (P <0 0 5 )。 (2 )A组和B组的QTd与SDNN、SDANN、SDNN index均存在着负相关 (P <0 0 5 )。结论　T2DM合并冠心病者心脏自主神经损害较单纯T2DM者重 ,前者迷走神经受损较后者更重。对T2DM及T2DM合并CHD患者常规 2 4h动态心电图检查 ,测量QTd是有益的 ,以便早期发现高危患者。     

Respiratory syncytial virus infections in previously healthy working adults.

During 1975-1995, a total of 2960 healthy adults, 18-60 years of age, were prospectively evaluated for respiratory virus infections. Of these subjects, 211 (7%) acquired respiratory syncytial virus (RSV) infection. The infections were symptomatic in 84% of subjects, involved only the upper respiratory tract in 74%, and included lower respiratory tract symptoms in 26%. Overall, 40% of the subjects were febrile. Lower respiratory tract signs developed in 26%. RSV illnesses were more prolonged than non-RSV respiratory illnesses. Compared with influenza, RSV infections were less frequently associated with fever and headache, but were associated significantly more often with nasal congestion, ear and sinus involvement, and productive cough. Absence from work during the acute phase of the illness resulted from 38% of RSV infections and 66% of influenza cases. The mean duration of RSV illness (9.5 days), however, was significantly longer than that of influenza (6.8 days). The occurrence of annual epidemics of RSV, the virus' potential to reinfect all age groups, and the morbidity associated with these reinfections suggest that RSV infections in working adults may result in appreciable costs for medical visits and absence from work.     

冠心病外周血白细胞PRKCB1 mRNA表达量与血管硬化危险因素及病变程度的相关性研究

目的 检测冠心病患者外周血白细胞蛋白激酶Cβ （PRKCB1）基因表达,分析其与冠心病类型、冠脉血管病变程度及动脉硬化危险因素血脂、高敏C-反应蛋白（hs-CRP）的关系.方法 纳入冠心病患者38例,其中稳定型心绞痛（SAP）10例、急性冠脉综合征（ACS）28例,另选健康对照组20名,进行血脂、hs-CRP水平、血细胞参数及PRKCB1 mRNA表达的检测分析.结果 SAP组和ACS组高密度脂蛋白胆固醇水平均较健康对照组显著降低（P＜0.05）,而甘油三酯水平高于健康对照组（P＜0.05, P＜0.01）,总胆固醇水平三组比较差异无统计学意义（P＞0.05）,SAP组和ACS组低密度脂蛋白胆固醇水平较健康对照组升高,ACS组升高差异有统计学意义（P＜0.05）.入院24 h后,与健康对照组相比,SAP组和ACS组患者hs-CRP水平升高,ACS组升高差异有统计学意义（P＜0.05）.SAP组和ACS组中性粒细胞计数均较健康对照组不同程度升高,ACS组升高差异有统计学意义（P＜0.05）.单核细胞计数和单核细胞百分比SAP组和ACS组均较健康对照组有升高趋势（P＞0.05）.冠心病患者PRKCB1 mRNA表达水平与健康对照者相比差异有统计学意义（P＜0.05）,且ACS组低于SAP组;PRKCB1 mRNA表达和血管病变支数呈负相关,单支、双支和三支血管病变组依次降低, 以三支血管病变组降低明显（P＜0.01）.结论 PRKCB1 mRNA表达与动脉粥样硬化危险因素明显相关,因冠心病的不同类型而有所差异,且与冠脉血管病变程度呈负相关.     

Ⅱ型糖尿病与非糖尿病患者经皮冠状动脉介入治疗特征的对比分析

目的: 比较冠心病（CHD）并发与不并发糖尿病（DM）患者行经皮冠状动脉介入治疗（PCI）操作特点,分析CHD并发DM患者冠状动脉病变特点,探讨临床上DM对PCI可能的影响。方法: 对2006年1月~2006年12月在天津市胸科医院依顺序进行的全部1 441例行PCI术并植入金属支架的患者进行回顾性分析,按是否伴有DM分为两组:DM组和非DM组。收集患者PCI治疗各项参数,包括病变类型、病变参数、植入支架参数等。应用SPSS10.0软件进行统计学检验,计数资料以 表示,P<0.05为差异有统计学意义。结果: 在1 441例行PCI的患者中,DM患者共计223人,占15.5%。 DM组C型病变比例明显高于非DM组（38.3% vs.19.7%;P<0.05）。DM组PCI治疗中治疗病变个数、植入支架个数明显多于非DM组（1.42±0.62 vs. 1.28±0.51;1.63±0.84 vs. 1.47±0.69,P<0.05）,而植入支架直径DM组明显小于非DM组［（3.00±0.42）mm vs. ( 3.06±0.43)mm,P<0.05］。其余PCI操作技术特点差异无统计学意义。结论: CHD并发DM行PCI术者病变较非DM者更重;CHD并发DM患者进行PCI治疗时需要处理更多病变、植入更多支架,且支架直径较小;CHD并发DM患者接受PCI治疗的比例与非并发DM的CHD患者相比更低。     

呼吸道合胞病毒对致敏小鼠T辅助细胞相关细胞因子表达的作用

目的　探讨经卵白蛋白 (OVA)雾化致敏的小鼠受呼吸道合胞病毒 (RSV)感染后肺内炎症发展的特点及与T辅助细胞有关的细胞因子的反应。方法　 40只小鼠随机分成 4组 ,每组 1 0只。对照组 :磷酸盐缓冲液 (PBS)雾化 1 0天 ,HEP 2细胞液滴鼻 ;RSV组 :PBS液雾化 ,RSV滴鼻 ;OVA组 :OVA致敏 ,HEP 2细胞液滴鼻 ;OVA +RSV组 :OVA致敏 ,RSV滴鼻。第 1 6天 ,每组各 6只小鼠支气管肺泡灌洗作细胞计数及分类 ,酶链免疫吸附试验 (ELISA)测定白细胞介素 4(IL 4)、γ干扰素 (IFN γ)的含量 ;余 4只取肺组织用于病理分析和提取总RNA ,经逆转录 聚合酶链反应 (RT PCR)测IL 4、IL 5、IFN γ的mRNA表达量。结果　 (1 )支气管肺泡灌洗液 (BALF)中细胞总数 :单纯RSV组为 (1 4 5±5 4)× 1 0 6 /ml、OVA +RSV组为 (1 6 8± 4 9)× 1 0 6 /ml,与对照组 [(7 7± 2 4)× 1 0 6 /ml]比较差异有显著性 (P <0 0 5) ;淋巴细胞RSV组为 0 63± 0 0 5、OVA +RSV组为 0 77± 0 0 9,与OVA组 (0 36± 0 0 3)、对照组 (0 2 8± 0 0 5)比较差异有显著性 (P <0 0 5) ;嗜酸细胞 :OVA +RSV组 (0 0 690± 0 0 1 0 0 )与其它三组 (0 0 0 30± 0 0 0 1 0、0 0 0 90± 0 0 0 50、0 0 1 0 0± 0 0 0 4 0 )比较差异也有显著性 (P均 <0     

冠心病和高脂血症红细胞PHGPx活性变化的意义

目的　通过测定红细胞中磷脂过氧化氢谷胱过氧化物酶 (PHGPx)和 Cu2 - Zn2 SOD活性和细胞膜氧化产物共轭双烯 (CD) ,探讨冠心病和高脂血症的发病机制。方法　分为三组 :A组 (冠心病不伴高脂血症患者 ) 36例 ,B组 (单纯高脂血症患者 ) 31例 ,C组 (冠心病伴有高脂血症患者 ) 32例 ,D组 (健康对照组 ) 30例。分别测定红细胞 PHGPx,Cu2 - Zn2 SOD,Na - K ATPase活性和 CD水平。结果　 A、B、C组红细胞 PHGPx,Cu2 - Zn2 SOD,Na - K ATP酶活性均显著低于 D组 (P<0 .0 5) ,而 CD水平均显著高于 D组 (P<0 .0 5)。A、B、C组间比较 Na - K ATP酶活性也有显著差异 (P<0 .0 5)。 PHGPx与 CD呈线性负相关 (r=- 0 .56,P<0 .0 5) ,PHGPx与 Na - K ATP酶呈线性正相关 (r=0 .68,P<0 .0 5)。结论　PHGPx活性变化与冠心病和高脂血症的发病密切相关。     

冠心病患者外周血单个核细胞HCMV-DNA的荧光定量PCR检测

目的了解冠心病(CHD)患者外周血单个核细胞(PBMCs)人巨细胞病毒(HCMV)-DNA存在情况并探讨其临床意义。方法应用实时荧光定量PCR(real time FQ-PCR)方法,对62例CHD患者,29例其他心血管疾病患者和30例健康体检者的PBMCs内HCMV-DNA进行定量检测。结果 CHD患者PBMCs的HCMV-DNA检出率41.9%,其他心血管疾病组10.3%,正常对照组10.0%,差异有统计学意义(P<0.05)。CHD组HCMV-DNA载量介于103~105拷贝/ml者为12例,占阳性例数的46.1%(12/26),介于105~106拷贝/ml者为8例,占阳性例数的30.7%(8/26)。结论 CHD患者PBMCs的HCMV感染可能在其致动脉粥样硬化性发病过程中发挥重要作用。