OBSERVATION ON THE THERAPEUTIC EFFECT OF CHILDREN''''S ASTHMA(386 CASES) TREATED WITH SALT-SEPARATED MOXIBUSTION IN THREE PERIODS OF HOT SUMMER DAYS

According to the theory of″relevant adaption of the human body to natural environment″and the principle of″treating winter syndrome in summer″in the traditional Chinsesmedicine,I selected Dazhui（GV 14）,Feishu（BL 13）and Yongquan（KI 1）as main points,onwhich putting a layer of fine salt,and a burning moxa cone over the salt,to treat children’s asthma.In addition,Shenshu（BL23）was added for the disease of kidney deficiency type,and Fenglong（ST40）for the one of phlegm-heat type.The total effective rate in 386 cases treated was 90.4％.The resultsshowed that the age of patient had no obvious relation to the therapeutic effect,but the effectwas better for the patients with short course of disease and kidney deficiency type.     

Analysis of similarities and differences of the twelve Jing-Well acupoints for emergency

The twelve Jing-Well acupoints are located at the tips of the limbs, with strong stimulation andintense needling sensation, and play important roles in the treatment of various critical diseases. The twelve Jing-Well acupoints share similarities and have specificities in the treatment of critical and severe diseases. The similarities of the twelve Jing-Well acupoints are supposed to open the orifices and remove blockage, clear away heat, resolve swelling, dissolve toxin and remove blood stasis. The Jing-Well acupoints locate at the different positions also have their specific effects for emergencies. Shaoshang （LU 11） is supposed to clean and benefit the throat. Shangyang （LI 1） is supposed to clear away heat and purge Fu organs. Lidui （ST 45） is supposed to expel fire and stop fear. Yinbai （SP 1） is supposed to stop bleeding and correct collapse. Shaochong （HT 9） is supposed to clear away heat from the heart and get rid of vexation. Shaoze （SI 1） is supposed to dredge the breast collateral. Zhiyin （BL 67） is supposed to promote birth and smoothen delivery. Yongquan （KI 1） is supposed to nourish yin and suppress yang. Zhongchong （PC 9） is supposed to benefit the orifice and brighten the eyes. Guanchong （TE 1） is supposed to clean and promote the triple energizer. Zuqiaoyin （GB 44） is supposed to induce menstruation and stop pain. Dadun （LR 1） is supposed to clear away the liver fire and relieve hernia. It is advisable to identify the similarities and differences and apply different stimulating modes in order to guide the clinical practice and enhance the emergency effect.     

Influence of electroacupuncture on COX activity of hippocampal mitochondria in senescence-accelerated mouse prone 8 mice

Objective： To observe the effect of electroacupuncture （EA） on cytochrome c oxidase （COX）activity of hippocampal mitochondria in senescence-accelerated mouse prone 8 （SAMP8） mice, and to explore the EA mechanism on Alzheimer disease （AD） in improving energy metabolic disorder.
Methods： Twelve SAMP8 mice were randomly divided into a model group and an EA group, with six in each group. Six senescence-accelerated mouse resistance 1 （SAMR1） mice were prepared as blank group. Mice in the EA group received EA on Baihui （GV 20） and Yongquan （KI 1）, once a day for 7 d as a course, altogether 3 courses with one day intervalbetween two courses. Mice in the model group and the blank group were manipulated and fixed as those in the EA group. After interventions, Morris water maze was employed to test spatial learning and memory ability to evaluate EA effect; spectrophotometry was used to detect the activity of hippocampal mitochondria COX.
Results： Compared with the blank group, mean escape latenciesof the EA group and model group were prolonged significantly in Morris water maze tests （P〈0.01）, the residue duration in the former platform quadrant significantly decreased （P〈0.01）. Compared with the model group, mean escape latencies on 1 d, 2 d and 3 d of the EA group were significantly reduced （P〈0.05）, and those on 4 d and 5 d continued the decreasing tendency （P〈0.01）, the residue duration on the former platform quadrant was significantly prolonged （P〈0.05）. The COX activity tests showed that, compared with the blank group, COX activities of the model group and the EA group were significantly decreased （P〈0.01）; compared with the model group, COX activity of the EA group was significantly elevated （P〈0.01）.
Conclusion： It＇s plausible that EA improves AD learning and memory ability by increasing mitochondria COX activity, protecting the structure and function, and improving energy metabolism.     

Clinical observation on electroacupuncture for post-stroke flaccid paralysis

Objective:To observe the clinical efficacy of electroacupuncture (EA) in treating post-stroke flaccid paralysis. Methods:Forty patients with post-stroke flaccid paralysis were randomized by the random number table into a treatment group and a control group, 20 cases in each. The treatment group was intervened by acupuncture at Jiquan (HT 1), Tianquan (PC 2), Ququan (LR 8), Yinlingquan (SP 9), and Yongquan (KI 1), and the control group was treated by acupuncture with conventional acupoint selection. Barthel index (BI) was adopted for evaluating the activities of daily living (ADL), and therapeutic efficacy was analyzed. Results:The two groups both had marked increases of BI score after treatment. Compared to the control group after 1 treatment course and 2 treatment courses respectively, the treatment group had significant differences in BI score (P<0.01). The total effective rate was 100.0% in the treatment group versus 90.0% in the control group, and the difference was statistically significant (P<0.01). Conclusion:EA at Jiquan (HT 1), Tianquan (PC 2), Ququan (LR 8), Yinlingquan (SP 9), Yongquan (KI 1) is an effective approach in treating post-stroke flaccid paralysis.     

针刺申脉、照海穴为主治疗失眠症52例

The writer adopted Shēnmài （申脉 BL62） and Zhàohǎi （照海 KI6） as the main points in the treatment of 52 cases of insomnia with acupuncture from 2005 to 2006 and had achieved satisfactory therapeutic effects. The following is the report.     

Effects of Hegu （LI 4） and Taichong （LR 3） on Vascular Dilation and Constriction in Migraine Patients

Hegu （LI 4） and Taichong （LR 3） are the Yuan- Primary acupoints of the Large Intestine and Liver Meridian, and are collectively named as Si Guan （four gates） point. Combined use of these two acupoints can harmonize yin and yang, regulate qi and blood, and balance ascending or descending. This paper attempts to study the effects of Si Guan point on the vascular dilation and constriction in migraine patients from the perspective of its feature.     

11 CASES OF MOTOR INJURY OF ANTERIOR TALOFIBULAR LIGAMENT TREATED BY ACUPUNCTURE OF QIUXU(GB 40)-THROUGH-ZHAOHAI(KI 6)POINT

Injury of ankle is the most common in athletic injury.Because of anatomic particu- larity of ankle joint,i.e.lateral malleolus is long and thin and is located at the posterior and lower than medial malleolus.The medial malleolus is broad and flat and is located at the anterior.Lateral ligament is thinner and weaker than medial ligament and the muscle groups supporting strephexopodia is far inferi- or to the muscle groups supporting strephenopodia in strength,injury of ankle joint happens more at inversion sprain and in- jury of anterior talofibular ligament is induced often.Thus,rapid and effective treatment of anterior talofibular ligament injury can elevate better athletics of sportsman.Whenever the disease happened in students to exercise,the authors often used acupuncture of Qiuxu（GB 40）-through-Zhaohai（KI 6）point with strong stimulative manipulation,which could relieve more remakedly pain of the ankle joint caused by anterior talofibular ligament injury as re- ported in the     

Chemical constituents from the stems of Gymnema sylvestre

AIM： To study the chemical constituents of stems of Gymnema sylvestre （Retz.） Schult. METHODS： Chromatographic techniques using silica gel, C18 reversed phase silica gel, and prep-HPLC were used. The structures were elucidated on the basis of MS and spectroscopic analysis （1D and 2D NMR）, as well as chemical methods. RESULTS： Seven compounds were isolated and their structures were elucidated as conduritol A （1）, stigmasterol （2）, lupeol （3）, stigmasterol-3-O-β-D-glucoside （4）, the sodium salt of 22α-hydroxy-longispinogenin-3-O-β-D-glucopyranosyl-（1→3）-β-D-glu-curono-pyranosyl-28-O-α-L-rhamnopyranoside （5）, oleanolic acid-3-O-β-D-glucopyranosyl-（1→6）-β-D-glucopyranoside （6）, and the sodium salt of 22α-hydroxy-longispinogenin 3-O-β-D-glucuronopyranosyl-28-O-α-L-rhamnopyranoside （7）. The inhibition activities of compounds 1, 5？7 on non-enzymatic glycation of protein in vitro were evaluated. CONCLUSION： Compound 7 is a new triterpenoid saponin. It was shown that compounds 1, 5？7 have weak inhibition activities for non-enzymatic glycation of protein in vitro.     

A new dimeric diarylheptanoid from the rhizomes of Alpinia officinarum简

AIM： To study the chemical constituents of the rhizomes ofAlpinia officinarum Hance. METHOD： Compounds were isolated by repeated column chromatography, and their structures were elucidated on the basis of spectral analysis. The cytotoxic activities of these compounds were evaluated with the T98G and B 16F10 cell lines by the MTT assay. RESULTS： A dimeric diarylheptanoid, named alpinin B （1）, along with three known diarylheptanoids were obtained, and their structures were identified as alpinin B （1）, 1, 7-diphenyl-3,5-heptanedione （2）, （4E）-1, 7-diphenylhept-4-en-3-one （3） and （4E）-7- （4-hydroxyphenyl）- 1-phenylhept-4-en-3-one （4）. CONCLUSION： Compound 1 is a new dimeric diarylheptanoid. The biosynthetic pathway of 1 was speculated to originate from a Michael reaction between compounds 2 and 3. Compound 3 showed cytotoxicity against the human glioblastoma T98G cell line with IC50 of 27 μmol·L-1.     

Volt-Ampere Characteristics of Acupoint Neiguan （PC 6） on Patients with Hyperthyroidism

Objective： To observe volt-ampere characteristic of Acupoint Neiguan （PC 6） on patients with hyperthyroidism for probing into the actions of acupoints and change of qi and blood of human body as well as the relationship between the action of acupoint and the metabolism of energy. Methods： A self-made high-sensitive apparatus was applied to detect volt-ampere curves of Acupoint Neiguan （PC 6） and its control point on 33 patients with hyperthyroidism and 30 healthy people. Results： In patients, the volt-ampere areas （VAA） of both ampere-increasing scan （AIS） and ampere-decreasing scan （ADS） as well as the inertia areas （IS） of both side Neiguan （PC 6） were significantly smaller than the control points （P〈0.05 or P〈0.01）.     

Effect of Zhuyeshigao granule on tumor necrosis factor-a and inter- leukins serum protein levels in rats with radiation esophagitis

OBJECTIVE： To investigate the effects of Zhuyesh- igao granule （ZSG） on tumor necrosis factor-a （TNF-a）, interleukin-1 （IL-1）, IL-2, IL-6, and IL-8 in rats with radiation esophagitis. METHODS： Fifty Wistar rats were randomly divid- ed into five groups （10 rats in each group）： con- trol （without radiation）, saline-treated, and low, medium, and high-dose ISG-treated groups. Rats were given normal saline （10 mL/kg） or 1.15, 2.3, or 4.6 g/kg ZSG by intragastric administration once a day for 7 days. A rat model of radiation esophagi- tis was established by local irradiation of Co60 （490.25 cGy/min, totaling 30 Gy）. The administra- tion of ZSG was continued for another 7 days and on the 7th day post-irradiation, inferior vena cavablood was collected. The serum was separated, and TNF-a, IL-1, IL-2, 11_-6, and IL-8 protein levels were determined. RESULTS： Inflammatory response factors were found in the serum of each group. However, levels in ZSG-treated groups were significantly lower than in the saline-treated group （P〈0.05）. CONCLUSION： ZSG may prevent the development of radiation esophagitis, perhaps by inhibiting the generation and release of the inflammatory re- sponse factors TNF-a, IL-1, IL-2, IL-6, and IL-8.     

Effects of Chinese herbs combined with in vitro fertilization and em- bryo transplantation on infertility： a clinical randomized controlled trial

OBJECTIVE： To assess the effects of using Chinese herbs in assisted reproductive technology. METHODS： Four hundred and thirty-three subjects aged less than 42 years with infertility due to Fallo- pian tube or male-related factors who were willing to undertake in vitro fertilization and embryo trans- plantation were randomly allocated to a Chinese herb intervention group （n=216） or a conventional treatment control group （n=217）. All subjects re- ceived one of four routine ultra-ovulation-promot- ing therapies at the Reproductive Center in the Third Hospital Affiliated to Beijing University ac- cording to their physician＇s assessments. The sub- jects in the intervention group received various Chi- nese herbs depending on their conventional treat- ment. Endometrial thickness, number of acquired eggs, and rates of normal fertility, high-quality em- bryos, biochemical and clinical pregnancy of sub- jects were assessed in both groups.RESULTS： The high-quality embryo rate of 51.9%, biochemical pregnancy rate of 51.0%, clinical preg- nancy rate of 44.2% and endometrial thickness of （10.84± 1.75） mm in the intervention group were all significantly higher than those in the control group [48.7%, 38.9%, 34.8%, and （10.52±1.50） mm, respec- tively; P〈O.05]. The normal fertility rate of 58.5% in the Chinese herb group was also significantly supe- rior to the 54.7% achieved in the control group （P〈 0.01）. There were no statistically significant differ- ences （P〉0.05） in the average number of acquired eggs within a single cycle, incidence of excessive stimulation of ovary, rates of embryo transplanta- tion or early abortion and birth of living babies be- tween the two groups. CONCLUSION： Our findings indicate that Chinese herbs increase endometrial thickness, improve the quality of fertility and embryo, and promote embry- onic nidation, thus enhancing the success rate of in vitro fertilization/intracytoplasmic sperm injec- tion-embryo transplantation cycle. Using Chinese herbs improves th     

Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal

OBJECTIVE： The purpose of this study was to evaluate the antioxidant activity, phenolic and flavonoids contents of selected medicinal plants form the Western region of Nepal.METHODS： The antioxidant activity of selected medicinal plants were determined by using 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity; total ferric ion reducing ability through spectroscopic analysis. The content of total phenols was determined using Folin-Ciocalteu reagent, whereas Aluminum chloride colorimetric method was used for flavonoid determination.RESULTS： Extracts of Syzygium Operculatus（87%）,Astilbe Rivularis（83%） and Mallotus Philippnensis（88%） showed significant free radical scavenging activity with effective concentration（EC50） close to that of ascorbic acid. Syzygium Operculatus（96%）,Astilbe Rivularis（97%） and Mallotus Philippnensis（97%） had potent reducing power in concentration dependent fashion. Those plant extract with higher free radical scavenging and ferric reducing effect also showed the greater content of both phenols and flavonoids, suggesting the correlation between polyphenolic content and antioxidant activity. Those plant extracts which showed better antioxidant activity assays, also demonstrated higher total phenol and flavonoid contents. These three plants showed the presence of higher amount of phenols and flavonoids.CONCLUSION： This study may provide the scientific basis for the traditional use of those plants and may provide valuable idea for further research.     

Reversal effect of bufalin on multidrug resistance in K562/VCR vin- cristine-resistant leukemia cell line

OBJECTIVE： To probe insights into the reversal effect of bufalin on vincristine-acquired multidrug resistance（MDR） in human leukemia cell line K562/VCR.METHODS： Proliferative inhibition rate and the reversal index（RI） of bufalin were determined by Methyl thiazolyl tetrazolium assay. The uptake of Adriamycin（ADM） in K562/VCR cells, cell cycle and apoptosis rate were determined by flow cytometry（FCM）. Cell morphologic changes were observed with Wright-Giemsa staining. The expression of P-glycoprotein（P-gp）, multidrug-associated protein-1（MRP1）, Bcl-x L and Bax protein were measured by immunocytochemistry.RESULTS： The human leukemia multidrug resistant K562/VCR cells showed no cross-resistance to bufalin. The RIs of bufalin at concentrations of 0.0002,0.001 and 0.005 μmol/L were 4.85, 6.94 and 14.77,respectively. Preincubation of 0.001 μmol/L bufalin for 2 h could increase intracellular ADM fluorescence intensity to 28.07%（P〈0.05） and down-regulate MRP1 expression simultaneously, but no remarkable effect was found on P-gp protein. Cell cycle analysis indicated increased apoptosis rate and apparent decreased G2/M phase proportion after treatment with bufalin. When exposed to 0.01μmol/L bufalin, typical morphological changes of apoptosis could be observed. Down-regulation of Bcl-x L and up-regulation of Bax expression in K562/VCR cells could be detected by immunocytochemistry.CONCLUSION： Bufalin could partly reverse the MDR of K562/VCR cells, with a possible mechanism of down-regulating MRP1 expression and activating apoptosis pathway by altering Bcl-x L/Bax ratio.     

Relationship between Renying pulse augmentation index and Cunk- ou pulse condition in different blood pressure groups

OBJECTIVE： To explore the relationship between Renying pulse （carotid） augmentation index （AI） and Cunkou pulse condition in different blood pres- sure groups, and the clinical significance of Reny- ing and Cunkou pulse parameters to reflect vascu- lar function. METHODS： Eighty-six patients with essential hyper- tension （EH） and 52 individuals with normal blood pressure （control group） between and January 2012 were included September 2010 this study. Reny- ing pulse AI was examined by a new diagnostic tool （ALOKA ProSound Alpha 10） --wave intensity （Wl） that is calculated as the product of the deriva- tives of the simultaneously recorded blood pres- sure changes （dP/dt） and blood-flow-velocity changes （dU/dt）, while Cunkou pulse condition was detected by DDMX-100 Pulse Apparatus inboth EH and control groups. A multifactorial corre- lation analysis was performed for data analysis. RESULTS： After adjusting for potential confound- ing variables, in the EH group, AI was positively cor- related with ts, w2/t （rts=0.225, P〈0.05; rw2/t=0.230, P〈 0.05） and negatively correlated with hs, hs/hl and w2 （rhs=- 0.393,P〈0.01 ;rhs/l=- 0.444, P〈0.01 ;rw2=- 0.389, P〈0.01）. In the control group, AI was positively cor- related with t3, t4, ts and w, （rt3=0.595, P〈0.01; r,4= 0.292, P〈0.05; rt5=0.318, P〈0.05; rw1=0.541, P〈0.01） and negatively correlated with h1, h2, h3, Ad and A （rh1= - 0.368, P〈0.05; rh2= - 0.330, P〈0.05; rh3= - 0.327, P〈 0.05; rAd=- 0.322, P〈0.05; rA=- 0.410, P〈0.01）. In the total sample group （EH plus control group, n= 138）, AI was positively correlated with t, ts, w1 and w, （rt=0.257, P〈0.01; rt5=0.266, P〈0.01; rw1=0.184, P〈 0.05; rw/t=0.210, P〈0.05） and negatively correlated with hs, hs/hl, w2 and Ad （rhs= - 0.230, P〈0.01; rh5/h1= - 0.218, P〈0.05; rw2= - 0.267, P〈0.01; rAd= - 0.246, P〈0.01）. Multiple linear regression analysis was car- ried out to model the relationship （F=7.887, P〈 0.001）.CONCLU     

Aqueous extract of Taxus Chinensis （Pilger） Rehd inhibits lung carci- noma A549 cells through the epidermal growth factor receptor/mi- togen-activated protein kinase pathway in vitro and in vivo

OBJECTIVE： To explore the anticancer mechanism of aqueous extract of Taxus Chinensis （Pilger） Rehd （AETC）. METHODS： The serum pharmacological method was used to avoid interference from administration of the crude medicinal herbs. Eight purebred NewZealand rabbits were used for preparation of serum containing various concentrations of AETC. For- ty-eight Balb/c-nu mice were used for in vivo experi- ments. The effects of serum containing AETC on the proliferation of A549 cells and expression levels of the epidermal growth factor receptor/mito- gen-activated protein kinase （EGFR/MAPK） path- way-related proteins in vitro were investigated. Ad- ditionally, the effects on the growth of A549 xeno- grafts in nude mice, and expression levels of the EG- FR/MAPK pathway-related proteins in the xeno- grafts, were investigated. RESULTS： 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl- tetrazolium bromide （MTT） assay revealed that the serum containing AETC significantly decreased the viability of A549 cells in a dose-dependent manner. Western blot showed that the serum containing various concentrations of AETC strongly reduced the levels of phospho-Jun N-terminal kinase （p-JNK） and phospho-extracellular signal-regulated kinasel/2 （ERK1/2） while it increased the level of p-p38. However, no significant effects on the ex- pression levels of JNK, ERK1/2, and p38 MAPK were found. In addition, an anticancer effect from AETC was observed in vivo in the Balb/c-nu mice bearing A549 xenografts.CONCLUSION： AETC has significant effects on the growth of A549 xenografts and on the activity of the EGFR/MAPK pathway. Therefore, AETC may be beneficial in lung carcinoma treatment.     

Xinfeng capsule improves pulmonary function in ankylosing spon- dylitis patients via NF-κB-iNOS-NO signaling pathway

OBJECTIVE： To study changes in the nuclear factor-κB p65（NF-κB p65）-inducible nitric oxide synthase（i NOS）-nitric oxide（NO） signaling pathway and the effects of Xinfeng capsules（XFC） in patients with ankylosing spondylitis（AS）METHODS： One hundred twenty patients with AS were randomly divided into an XFC group and a Salazopyrin group. Sixty health subjects were included as a normal control group. In the two treatment groups, pulmonary functional parameters,forced vital capacity（FVC）, forced expiratory volume in 1 second（FEV1）, maximal voluntary ventilation（MVV）, peak expiratory flow（PEF）, forced expiratory flow at 25% of forced vital capacity（FEF25）,forced expiratory flow at 50% of forced vital capacity（FEF50）, and forced expiratory flow at 75% of forced vital capacity（FEF75） were determined. Enzyme linked immunosorbent assays were used for detection of the serum oxidative stress indexes,NF-κB p65, i NOS, NO, reactive oxygen species（ROS）, reactive nitrogen species（RNS）, malondialdehyde（MDA）, superoxide dismutase（SOD）, catalase（CAT）, total antioxidative capacity（TAOC） and interleukin-4（IL-4）, IL-10, IL-1β, and tumor necrosis factor-α（TNF-α） contents. Westergren＇s method was used for determination of erythrocyte sedimentation rate（ESR）. High-sensitivity C-reactive protein（Hs-CRP） was detected with a 7060 full-automatic biochemical analyzer（Hitachi, Japan）.RESULTS： The clinical therapeutic effect in the XFC group was significantly superior to that in the Salazopyrin group（P〈0.01）. Compared with the normal control group, FEV1, MVV, PEF, FEF50, FEF75, SOD, CAT,TAOC, IL-4, IL-10 were significantly lower, and NF-κB p65, i NOS, NO, ROS, RNS, MDA, IL-1β, TNF-α, ESR,and Hs-CRP significantly higher in patients with AS（P〈0.01 or P〈0.05）. Compared with before treatment, FEV1, MVV, PEF, FEF50, FEF75, SOD, CAT, TAOC,IL-4, and IL-10 were significantly increased, and NF-κB p65, i NOS, NO, ROS, RNS, MDA, IL-1?     

Protective effect of Tanreqing injection on axon myelin damage in the brain of mouse model for experimental autoimmune encephalo- myelitis

OBJECTIVE： To evaluate the effect of Tanreqing injection on axon myelin in the mouse brain of experimental autoimmune encephalomyelitis（EAE）.METHODS： An EAE model was established by myelin oligodendrocyte glycoprotein（MOG）35-55 immunization in C57BL/6 mice. Mice were randomly divided into the following groups： normal, model,prednisone acetate（PA）（6 mg/kg）, Tanreqing high dose（5.14 m L/kg）, Tanreqing low dose（2.57 m L/kg）. On the day of immunization, both Tanreqing groups were treated by intraperitoneal injection,with the PA group treated by intragastrical perfusion after T cell response, and the other groups treated with saline. Changes in body weight, neurological deficit score, incidence rate, mortality rate,and course of disease were observed for all mice.Brain tissue was isolated and stained with hematoxylin-eosin, and pathological investigations performed to evaluate axon myelin damage by transmission electron microscopy（TEM）. Myelin basic protein and microtubule associated protein-2 were analyzed by immunohistochemistry.RESULTS： Tanreqing injection significantly prolonged EAE latency and decreased the neurological deficit score, alleviated infiltration of inflammatory cells in the focus area, up-regulated hippocampal MBP expression at the acute stage and the remission stage, and increased microtubule associated protein-2 expression in the EAE brain to varying degrees in the acute stage. TEM analysis indicated that Tanreqing injection alleviates myelin damage in the EAE mouse and maintains the integrity of circular layer structures and alleviates axon mitochondrial swelling.CONCLUSION： Tanreqing injection alleviates EAE symptoms.