大豆异黄酮对去卵巢大鼠脂、骨代谢的影响

目的 利用去卵巢大鼠作为绝经后动物模型，探讨大豆异黄酮对脂代谢、骨代谢的影响。方法 将成年雌性大鼠腹部手术切除双侧卵巢后，给予不同剂量的大豆异黄酮，喂养4个月后，分离血清进行分析指标测定。结果 大豆异黄酮可提高血清中的HDL和骨形成指标AKT、BGP的浓度；降低血清中的TC和骨吸收指标抗酒石酸酸性磷酸酶(STrACP)的浓度；对体内雌激素水平的影响较低。结论 大豆异黄酮对实验大鼠的脂代谢、骨代谢有一定的调节作用。     

大豆异黄酮对去卵巢大鼠骨力学性能的影响

目的　探讨植物雌激素大豆异黄酮对大鼠去侧巢后骨密度和骨力学指标变化的影响。方法　腹腔手术切除大鼠双侧卵巢 ,分为去卵巢对照组、大豆异黄酮剂量组和雌激素对照组 ,并设假手术对照组。喂养 16周后 ,分离股骨 ,双能量X射线骨密度仪测定骨密度 (BMD) ,三点弯曲实验法测定骨力学指标弯曲强度 (Flexuralstrengh)和弯曲弹性模量 (Flexuralelasticmodule) ,分析天平称量骨的重量。结果　大鼠去卵巢后骨密度显著下降 ,股骨的力学性能指标有较大变化。给予大豆异黄酮和雌激素后 ,可使骨密度显著提高 (P <0 0 1) ,存在一定的剂量 -效应关系 ,弯曲强度和弯曲弹性模量明显增加。雌激素组 (EC)大鼠的股骨长度和不同状态下的骨重量显著降低 ,其它各组之间经统计学分析无显著性变化。结论　大豆异黄酮和雌激素可增加去卵巢大鼠股骨骨密度、改善骨的生物力学性能     

Effects of soybean isoflavone dosage and exercise on the serum markers of bone metabolism in ovariectomized rats

This study was designed to determine whether combined treatments with soybean isoflavone dosage and moderate exercise would exhibit synergistically effects on bone metabolism following the onset of menopause. Fifty 12 wk-old female Wistar rats were assigned to five groups: 1) Sham operated (Sham), 2) ovariectomized (OVX), 3) OVX received soybean isoflavone (OVX-IF), 4) OVX exercised (OVX-EXE) and 5) OVX treated with both soybean isoflavone and exercise (OVX-IF-EXE). All rats were fed a normal diet ad libitum. Daily soybean isoflavone dosage was 50 mg/kg body weight. The vehicle was given in Sham, OVX and OVX-EXE groups. The drugs were all oral administered using a stomach tube. Exercising rats were trained on an uphill treadmill at 20 m/min for 1h/day, 5 days/week. The experimental duration consisted of the adaptation periods of 2 weeks and treatment periods of 8 weeks. The results showed that the uterus relative weights in OVX-EXE, OVX-IF and OVX-IF-EXE groups were all lower than those in Sham, they were higher than those in the OVX group. Serum alkaline phosphates (AKP) activities of OVX was significantly increased as compared to that of Sham (p<0.01). OVX-IF and OVX-IF-EXE respectively decreased the Serum alkaline phosphates activities, as compared to that of OVX (p<0.01). The tartrate-resistant acid phosphatase (TRAP) value of OVX was significantly increased as compared to that of Sham (p<0.05). OVX-IF decreased the TRAP as compared to that of OVX (p<0.05). These results suggest soybean isoflavone and resistance exercise both can restrain ovx-induced bone loss. But their mechanisms may be different.     

Combined effects of soy isoflavone and lecithin on bone loss in ovariectomized mice

BACKGROUND/OBJECTIVESIsoflavones (ISFs) are effective in preventing bone loss, but not effective enough to prevent osteoporosis. Mixtures of soy ISF and lecithin (LCT) were prepared and characterized in an attempt to improve the bone loss.MATERIALS/METHODSThe daidzein (DZ) and genistein (GN) solubility in soy ISF were measured using liquid chromatography-mass spectrometry. The change in the crystalline characteristics of soy ISF in LCT was evaluated using X-ray diffraction analysis. Pharmacokinetic studies were conducted to evaluate and compare ISF bioavailability. Animal studies with ovariectomized (OVX) mice were carried out to estimate the effects on bone loss. The Student''s t-test was used to evaluate statistical significance.RESULTSThe solubility of DZ and GN in LCT was 125.6 and 9.7 mg/L, respectively, which were approximately 25 and 7 times higher, respectively, than those in water. The bioavailability determined by the area under the curve of DZ for the oral administration (400 mg/kg) of soy ISF alone and the soy ISF-LCT mixture was 13.19 and 16.09 µg·h/mL, respectively. The bone mineral density of OVX mice given soy ISF-LCT mixtures at ISF doses of 60 and 100 mg/kg daily was 0.189 ± 0.020 and 0.194 ± 0.010 g/mm³, respectively, whereas that of mice given 100 mg/kg soy ISF was 0.172 ± 0.028 g/mm³. The number of osteoclasts per bone perimeter was reduced by the simultaneous administration of soy ISF and LCT.CONCLUSIONSThe effect of preventing bone loss and osteoclast formation by ingesting soy ISF and LCT at the same time was superior to soy ISF alone as the bioavailability of ISF may have been improved by the emulsification and solvation of LCT. These results suggest the possibility of using the combination of soy ISF and LCT to prevent osteoporosis.     

大豆异黄酮对去卵巢大鼠骨钙、骨强度的影响

目的　利用卵巢切除大鼠模型 ,研究大豆异黄酮对体内骨转换、雌二醇、骨钙含量及骨强度的影响 ,探讨大豆异黄酮的剂量 效应关系。方法　卵巢切除大鼠分为 3个剂量组 ,分别灌胃给与 12 . 6 5 (L SI,低剂量组 ) ,2 5 . 30(M SI ,中剂量组 ) ,37. 95mg/(kg·bw) (H SI ,高剂量组 )。同时设阴性对照组 (OVX)和假手术组 (Sham) ,以蒸馏水灌胃。喂养 12周后 ,处死大鼠 ,分离血清 ,同时剥离右侧股骨及右侧胫骨 ,进行各项指标的测定。结果　OVX组骨形成指标碱性磷酸酶 (AKP)比Sham组及H SI组显著升高 (P <0. 0 5 ) ,其骨吸收指标抗酒石酸酸性磷酸酶 (StrACP)显著性高于Sham组、M SI组及H SI组 (P <0 . 0 5 )。Sham组及各大豆异黄酮剂量组雌二醇 (E2 )有上升趋势 ,中剂量组(M SI)E2 有显著性上升 (P <0 . 0 5 )。Sham组与大豆异黄酮各剂量组内钙含量与OVX组相比显著增高 ,反映骨强度性能的指标 (最大负载、弹性系数及能量 )也较OVX组明显增强。结论　大豆异黄酮能显著改善但不能完全逆转业已形成的骨质疏松。     

大豆异黄酮对去势大鼠骨代谢的影响

[目的]探讨大豆异黄酮改善激素缺乏大鼠骨营养状况及作用机制。[方法]选用卵巢切除大鼠所诱发的骨质疏松模型,给予大豆异黄酮治疗,同时设假手术组及模型对照组。3个月后测定大鼠骨密度、骨钙含量及骨代谢酶学指标。[结果]大豆异黄酮可提高去势大鼠的骨密度、骨钙含量;降低血清碱性磷酸酶(AKP)及抗酒石酸酸性磷酸酶(TRACP)的活性(P<0.05),其作用与剂量有关。[结论]大豆异黄酮能够增加去势大鼠的骨密度,有类似雌激素样作用,可有效防治卵巢切除大鼠的骨量丢失,其作用机制可能是通过降低骨转换率来实现。     

Effects of tuna bone and herbal extract on bone metabolism in ovariectomized rats

This study was conducted to examine the effects of herb extract addition on bone metabolism of ovariectomized (OVX) rats fed a diet with tuna bone as a source of calcium. The experimental groups were sham–control (CaCO₃ [CC]), OVX-CC (OVX-control), OVX–CC + herb extract, OVX–tuna bone powder (TB), OVX–tuna bone powder + herb extract, OVX–citrated tuna bone powder (CTB), and OVX–citrated tuna bone powder + herb extract (CTBH). The herb extract included Rehmanniae radix and Angelicae gigantis radix, following the prescribed theories of Oriental herb medicine. In the OVX-CTBH group, the level of fecal (33.6%) and urinary calcium excretion (45.8%) greatly decreased compared with the TB groups. Osteocalcin concentrations were significantly lower in the OVX-CC group and higher in the OVX-CTB group. The deoxypyridinoline value was significantly influenced by ovariectomy. It was elevated in all OVX groups compared with the sham group, and it was reduced by 12.8% in rats supplemented with the herb extract. The bone density value per 100-g body weight was lowest in the TB group, and it was higher in the tuna bone powder + herb extract, CTB, and CTBH groups. We concluded that TB can be a good calcium source, and it was effective in maintaining skeletal metabolism of OVX rats when it was fed in the form of citrated or herb extract–supplemented diets.     

大豆异黄酮对雄性子代大鼠生殖系统影响

目的 探讨雌性孕期大鼠大豆异黄酮暴露对雄性子代生殖系统的影响。方法 SD雌性大鼠按2:1比例与雄性大鼠同笼交配获取40只孕鼠。将孕鼠随机分为4组,在孕期13~19 d将大豆异黄酮以花生油为溶剂灌胃染毒,子一代雄鼠继续饲喂至90 d剖杀。结果 150及450 mg/kg大豆异黄酮组子代大鼠睾丸重量分别为2.68和216g,碱性磷酸酶活性分别为1 078.88和1058.88U乳酸脱氢酶水平分别为2 678.88和1 529.38U,精子活动度分别为20.12%和2 413%,附睾尾精子计数分别为50×10⁶g和40.5×10⁶%,睾丸每日精子生成量分别为19.46106/(g·d)和6.21106/(g·d),与对照组比较均显著下降(P<0.05)且表现出剂量-效应关系。结论 大鼠孕期较大剂量大豆异黄酮暴露对雄性子代生殖系统具有损伤作用。     

雌激素对去卵巢大鼠生长发育及骨代谢影响

目的 探讨雌激素对去卵巢大鼠生长发育、血液生化指标、骨营养状况及子宫增生的影响及作用机制.方法 4月龄雌性SD大鼠腹腔假手术或切除双侧卵巢,分为假手术组(Sham)、去卵巢对照组(0vx)和雌激素组(EC).用自行配制的含有β-雌二醇的饲料喂养7周,实验期间记录各组大鼠体重和摄食量,并计算食物利用率.实验结束后测定大鼠血清生化指标、股骨密度、骨钙含量,同时对子宫进行病理组织学观察.结果 实验结束时雌激素组体重、食物利用率明显低于去卵巢对照组(P<0.01);雌激素组大鼠骨密度均明显高于去卵巢对照组(P<0.05);雌激素组大鼠子宫未见明显萎缩.结论 雌激素可显著抑制大鼠去卵巢后体重增加,并有效减少骨量丢失,增强骨密度,对骨钙含量有增加趋势;同时对子宫的增生作用非常明显.     

大豆异黄酮对去卵巢大鼠脂类代谢和抗氧化作用的影响

目的:探讨大豆异黄酮(SI)对去卵巢大鼠血清脂类代谢和脂蛋白(a)及抗氧化指标的影响。方法:将63只雌性SD大鼠摘除双侧卵巢,术后恢复1周,随机分为高脂对照组(HF组)、雌激素高脂饲料组(EG组)、低剂量SI高脂饲料组(L-SI组)、中剂量SI高脂饲料组(M-SI组)、高剂量SI高脂饲料组(H-SI组)、假手术组(Sham组)和正常对照组(NG组)共7组,灌胃给药3个月,每周称重一次,分别在大鼠去卵巢时、给药4、8、12 w抽取尾静脉血(每次采尾血前空腹12 h),分别测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、脂蛋白a[Lp(a)]、抗氧化指标及骨密度等,实验结束后取内脏及骨骼标本。结果:SI各干预组与干预前比较TC、HDL-C、LDL-C显著增高(P均<0.01),H-SI组Lp(a)低于干预前(P<0.05)。SI干预后不同组间TG、LDL-C、Lp(a)改变显著(P均<0.01),LDL-C项各干预组明显低于HF组,但高于Sham组。干预后各组超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)活性均有升高(P均<0.01)。结论:适量SI对去卵巢大鼠的高脂血症存在拮抗作用并能改善体内的脂质过氧化,尤其在应激阶段效果更佳。     

Effects of a fermented vegetable product on fat deposition and bone metabolism in ovariectomized rats

We examined the effects of a fermented vegetable product (FVP), fermented by yeast as well as lactic acid bacteria, on body weight and fat deposition after ovariectomy in 10-wk-old Sprague-Dawley rats. The rats were divided into the following 5 groups: (1) ovari-ectomized rats fed as usual (OVX); (2) ones receiving FVP at a daily dose of 100 mg/kg (LOW); (3) ones receiving FVP at a daily dose of 1000 mg/kg (HIGH); (4) ones receiving a calcium-free diet (Ca-FREE); (5) sham-operated rats (SHAM). A slight increase in body weight was observed in the LOW group compared with the OVA group. The rate of increase in total fat content was lowest in the LOW group, but both bone weight and strength were similar to those in the other ovariectomized groups. In conclusion, the low dose of FVP turned out to reduce fat content without affecting bone weight and strength.     

Consumption of soy protein reduces cholesterol absorption compared to casein protein alone or supplemented with an isoflavone extract or conjugated equine estrogen in ovariectomized cynomolgus monkeys

Dietary intake of soy protein is associated with reductions in plasma cholesterol. Isoflavones are thought to be active components of soy and responsible for the beneficial effects because of their structural similarities to estrogen. The purposes of this study were to determine if i) soy protein or a semipurified soy extract, rich in isoflavones, is responsible for improving the lipid profile and ii) altered intestinal cholesterol metabolism is one mechanism for hypocholesterolemic effects. Ovariectomized adult female cynomolgus monkeys (40) were assigned to groups fed diets containing i) casein-lactalbumin (CAS) ii) intact soy protein (SOY), iii) CAS plus an isoflavone-rich semipurified soy extract similar in isoflavone content as SOY (ISO) or iv) CAS plus conjugated equine estrogen (CEE) for 20 wk. Cholesterol absorption was determined using the fecal isotope ratio method. Bile acid excretion was measured using the 3alpha-hydroxysteroid dehydrogenase assay. The SOY group had significantly lower total- and VLDL + LDL-cholesterol compared to the other three groups and significantly higher HDL-cholesterol compared to the CAS and CEE groups. Cholesterol absorption was significantly lower in the SOY group compared to the other groups, but bile acid excretion was not significantly affected. The hypocholesterolemic effect of soy protein appears to be mediated in part by decreased cholesterol absorption. The semipurified soy extract, rich in isoflavones, added to casein protein did not have lipid-lowering effects. Other components of soy such as saponins, phytic acid or the amino acid composition may be involved in the hypocholesterolemic effects seen in this study.     

大豆异黄酮对去卵巢大鼠体重及食物利用率的影响

目的研究植物雌激素大豆异黄酮对模拟妇女绝经的去卵巢大鼠体重增加的抑制作用及其降低体内食物利用率的作用。方法4月龄雌性Wistar大鼠腹腔假手术或切除双侧卵巢,分为假手术对照组(Sham)、去卵巢对照组(Ovx)、雌激素对照组(EC)和3个大豆异黄酮剂量组,另设正常对照组。用自行配制的含有己烯雌酚和不同剂量大豆异黄酮的饲料喂养16周,实验期间记录各组大鼠的体重和摄食情况,并计算食物利用率。结果高剂量大豆异黄酮(187.4mgkgbw·d)和己烯雌酚可显著抑制大鼠去卵巢后的体重增加;剂量降低,抑制作用减弱。与Sham组和Ovx组相比,大豆异黄酮组大鼠摄食量显著降低,但3个剂量组间没有差异且高于EC组。与OVX组相比,高剂量大豆异黄酮组大鼠食物利用率显著降低,但高于EC组。大豆异黄酮对大鼠的生长状态及脏体比值没有影响。结论高剂量大豆异黄酮可通过降低体内食物利用率而显著抑制大鼠去卵巢后体重的增加。     

Combined effects of exercise and soy isoflavone diet on paraoxonase, nitric oxide and aortic apoptosis in ovariectomized rats

Menopause is associated with an increased risk of cardiovascular disease (CVD). Exercise and soy isoflavone diets have been suggested to reduce the risk of CVD in postmenopausal women. We investigated the effects of exercise, or combined exercise and soy isoflavone diet, on plasma lipid profiles, paraoxonase (PON), nitric oxide (NO) and apoptosis in the aorta of ovariectomized (OVX) rats. Thirty-two female Sprague-Dawley rats were divided into four groups: OVX with general diet (OVX-GD), OVX with isoflavone diet (OVX-ISO), OVX-GD with exercise training (OVX-ET) and OVX-ISO with exercise training (OVX-ISO+ET). The experimental rats undertook treadmill training (30 min/day, 4 days/week) and/or were supplied a soy isoflavone diet (added to the experimental diet at 2.39 mg/g protein) for 12 weeks. Body weight and levels of total cholesterol (TC), triglyceride (TG), LDL-cholesterol (LDL-C) increased in the OVX rats and HDL-C decreased. These effects were reduced by exercise and/or soy isoflavone supplementation. PON and NO activities were higher in the OVX-ISO+ET group than in the OVX-GD group. In addition, this group had lower caspase-9 and -3 and higher Bcl-2 expression, and there was less aortic apoptotic cell death. These results suggest that a combination of exercise and a soy isoflavone diet has beneficial effects in terms of protecting against cardiovascular risk factors by controlling lipid profiles and the related enzyme, PON, as well as NO activity and apoptosis of the aorta in OVX rats.     

大豆异黄酮对高胆固醇模型大鼠血清胆固醇浓度和肝脏胆固醇代谢的影响

目的观察大豆异黄酮对高脂模型大鼠血清胆固醇浓度及其肝脏胆固醇代谢关键酶基因表达的影响。方法 9周龄SD雄性大鼠8只喂饲AIN93M饲料（阴性对照组）,18只喂饲高脂饲料建立高胆固醇模型。14 d后将高胆固醇大鼠随机分为异黄酮组（每天灌胃360 mg/kg BW大豆异黄酮）和高脂对照组,喂饲高脂饲料至实验结束。30 d后测血脂,实时定量聚合酶链式反应（polymerase chain reaction,PCR）测定肝脏羟甲戊二酰辅酶A还原酶（3-hydroxy-3-methylglutaryl-Coenzyme A reductase,Hmgcr）、胆固醇7α-羟化酶（cytochrome P450,family 7,subfamily a,polypeptide 1,Cyp7a1）、低密度脂蛋白受体（low density lipoprotein receptor,Ldlr）mRNA表达量。结果异黄酮组大鼠血清总胆固醇（total cholesterol,TC）和低密度脂蛋白胆固醇（low density lipoprotein cholesterol,LDLC）浓度分别比高脂对照组降低了23.7%（q=2.79,P=0.010）和23.2%（q=2.63,P=0.015）;动物肝脏的Hmgcr和Cyp7a1 mRNA表达量均出现下降,但差异无统计学意义（均有P〉0.05）。结论大豆异黄酮可明显降低高胆固醇大鼠血清TC和LDLC浓度,有可能影响肝脏胆固醇代谢。     

大豆异黄酮对去卵巢大鼠血脂及骨生化指标的影响研究

探讨大豆异黄酮对去卵巢大鼠血清中血脂指标、骨代谢生化指标及激素水平的影响。选择 4月龄的Wistar大鼠 ,根据体重随机分为 6组。除 1组行单纯开腹手术外 ,其它 5组均切除双侧卵巢 ,分别为 :去卵巢对照组 ;高剂量大豆异黄酮组 ;中剂量大豆异黄酮组和低剂量大豆异黄酮组 ;雌激素组。喂养 16周后 ,处死大鼠 ,分离血清进行分析指标的测定。结果发现 :给予去卵巢大鼠大豆异黄酮后 ,血清中高密度脂蛋白(HDL)含量与去卵巢对照组相比有所增高 (P <0 0 5 ) ;总胆固醇 (TC)、甘油三酯 (TG)指标下降 ;骨吸收指标显著降低 ,骨形成指标骨钙素水平与去卵巢组比有所提高 ;同时 ,大鼠切除卵巢后 ,体内雌激素水平显著下降。结果显示 :大豆异黄酮对去卵巢大鼠的血脂有一定的调节作用 ,对骨吸收有显著的抑制作用     

大豆异黄酮及雌激素对去势大鼠体重和ghrelin影响

目的探讨大豆异黄酮和雌激素对去卵巢大鼠体重、血脂和生长激素释放肽(ghrelin)水平影响。方法 28只去卵巢SD大鼠随机分为4组,分别给予基础膳食(AIN-93 G),基础膳食+低、高剂量异黄酮(0.4、1.6 g/kg)和雌激素(0.15 g/kg),定期测量大鼠体重。12周后,腹腔麻醉大鼠心脏取血,分离血清,分别测定血清血脂、脂蛋白、gh-relin和脂联素水平;分离脏器计算脏器系数。结果实验期间,对照组和大豆异黄酮组动物体重均明显增加,而雌激素组动物体重在实验期间相对稳定;高剂量异黄酮组和雌激素组大鼠体重增加量(48.67、2.10g)明显低于对照组(95.29g)和低剂量异黄酮组(89.54g)(P<0.05)。雌激素组大鼠子宫/体重比明显高于对照组(P<0.01);高剂量大豆异黄酮组与雌激素组大鼠血清ghrelin水平分别为(3.54±0.82)和(2.78±0.27)ng/mL,明显低于对照组(5.03±1.49)ng/mL(P<0.05);未发现大豆异黄酮和雌激素对血脂、游离脂肪酸和脂联素水平影响。结论大豆异黄酮和雌激素均能抑制去卵巢大鼠体重增长,其作用可能与调节血清ghrelin水平有关。     

微囊化大鼠卵巢细胞对去卵巢大鼠骨代谢的影响

目的:观察去卵巢大鼠微囊化卵巢细胞移植后不同时期的骨代谢指标的变化,探讨微囊化卵巢细胞作为防治绝经后妇女骨质疏松方法的可行性。方法:将12周龄SD雌性大鼠36只随机分成假手术组(SHAM组)、去卵巢组(OVX组)和微囊化卵巢细胞移植组(MOVT组)。术后10天MOVT组大鼠腹腔注射微囊化卵巢细胞2 ml,SHAM组和OVX组注射0.9%NaCl溶液2 ml,观察12周;术后4、8、12周尾静脉采血测定血清E2及骨代谢指标ALP、TRAP、OC、HYP的浓度或活性。结果:与SHAM组大鼠比较,OVX组大鼠血清E2在术后4周即有非常显著的下降(P<0.01),且随着时间延长而持续下降;OVX组大鼠血清TRAP、HYP、ALP、OC水平在术后均显著升高,其中TRAP、HYP在术后8周达到最高峰(P<0.01);而ALP、OC则在术后12周达到最高峰(P<0.01)。与OVX组大鼠比较,MOVT组大鼠血清中E2水平在移植后4周显著升高(P<0.05),移植后8周、12周差异显著(P<0.01);血清TRAP和HYP水平在移植后4周即明显降低(P<0.05),到移植后8周差异有显著性意义(P<0.01);而ALP和OC水平在移植后8周开始降低,其中ALP更加显著(P<0.05),到移植后12周达到最小值(P<0.01)。结论:微囊化卵巢细胞移植具有预防去卵巢大鼠骨质疏松发生的作用,微囊化卵巢细胞移植作为临床防治绝经后骨质疏松的一种手段具有良好的应用前景。     

大豆异黄酮对更年期妇女骨密度影响

目的 拟通过人群膳食干预试验,从细胞因子水平研究植物雌激素大豆异黄酮对更年期妇女骨密度的作用。方法 随机双盲法将90名更年期妇女分为大豆异黄酮组观察和安慰剂对照组,每人每天服用大豆异黄酮90mg或者安慰剂,试验期限6个月。超声法测定爱试对象桡骨远端和胫肌中段的骨密度;酶联免疫学方法测定受试对象血清中细胞因子白介素-6(II-6)和肿瘤坏死因子-α(TNF-α)的水平。结果 服用大豆异酮组受试对象的胫骨骨密度增加明显,差异有统计学意义,安慰剂组受试对象骨密度差异无统计学意义;大豆异黄酮组受试对象血清中IL-6和TNF-α的水平在试验结束后均显著降低,差异有统计学意义(P<0.05)。结论 植物雌激素大豆异黄酮可有效增加更年期妇女骨骼密度,其机制可能是通过降低血清中细胞因子IL-6和TNF-α的水平实现的。