Serum IL-4, IL-10 and IL-6 levels in inflammatory arthritis

As the available in vitro and in vivo data suggest that interleukin (IL)-4 and IL-10 have immunosuppressive activity, our hypothesis was that serum IL-4 and IL-10 levels would correlate inversely with parameters of inflammation in patients with inflammatory arthritis. IL-4 was detected in the serum of 12 out of 140 patients with rheumatoid arthritis (RA), which was increased compared to the proportion found with patients with osteoarthritis (OA; P< 0.02). In addition, IL-4 was detected in the serum of 2 of 19 patients with systemic lupus erythematosus (SLE), 2 of 24 patients with psoriatic arthritis and 1 of 5 patients with Behçet's syndrome. No IL-4 was detected in patients with the following conditions: OA (58 patients), gout (17 patients), ankylosing spondylitis (6 patients), Reiter's syndrome (6 patients), polymyalgia rheumatica (6 patients), temporal arteritis (5 patients) and scleroderma (3 patients). No IL-10 was detected in any of the sera tested. We discuss the possible relevance of these results to the regulation of the immune response evident in inflammatory arthritis.     

老年肺癌患者IL-6、sIL-2R和T细胞亚群检测的临床意义

目的　探讨老年肺癌患者外周血 IL - 6、s IL- 2 R和 T细胞亚群活性的变化。方法　对老年肺癌患者化疗前、后及老年健康对照组应用流式细胞分析法测定 T细胞亚群 ,双抗体夹心法 (EL ISA)测定 IL - 6及 s IL- 2 R水平。结果　肺癌组 s IL - 2 R、IL- 6及 CD8高于对照组 (P<0 .0 5) ,而CD3 及 CD4水平低于对照组 ,化疗后 s IL - 2 R、IL- 6及 CD8水平明显下降 ,但仍高于对照组 ,CD3 及 CD4水平略升高 ,仍低于对照组。结论　 IL - 6、s IL-2 R及 T细胞亚群联合测定可以反映肺癌病人机体免疫功能状态 ,可作为判断疾病的预后和免疫调节治疗的客观指标。     

百草枯中毒患者的IL-6、sIL-6R、sgp130水平及其与患者预后的相关性分析

目的 探讨百草枯中毒患者的IL-6、sIL-6R、sgp130表达水平及其与患者预后的关系.方法 选取2017年1月至2019年7月我院收治的百草枯中毒患者60例作为中毒组,选择同期来我院进行健康体检的志愿者60名为对照组.根据患者的预后情况将中毒组患者分为预后良好组(38例)和预后不良组(22例).比较中毒组患者和对...     

The combination of IL-6 and its soluble receptor is associated with the response of rheumatoid arthritis patients to tocilizumab

BackgroundIL-6 contributes significantly to the chronic inflammatory process of rheumatoid arthritis (RA). Tocilizumab, a humanized anti-human IL-6 receptor antibody that blocks the signaling originated by the IL-6/IL-6R complex, is an effective treatment. However, predictors of the response to tocilizumab are still required. We aimed to combine IL-6 and soluble IL-6R (sIL-6R) levels to identify groups of responses.MethodsHeparinized blood and clinical data from 63 RA patients were collected before treatment and after 3 and 6 months. Two-step clustering (SPSS v.18) was used to establish the relationship between IL-6 and sIL-6R. Then, we compared European League Against Rheumatism (EULAR) response criteria with remission achievement in the groups of patients.ResultsThree statistical significant clusters of RA patients (i.e., g1, g2, and g3) were defined by serum concentrations of IL-6 and sIL-6R at baseline. All groups of RA patients had higher IL-6 and sIL-6R levels than healthy donors. The levels of IL-6 expressed as median (IQR) in g1 patients were 124(90–183) pg/ml, in g2 12.3(4.4–24) pg/ml, and in g3 60.1(30–146) pg/ml (p < 0.001). The levels of sIL-6R expressed as mean ± sd in g1 patients were 250.5 ± 72 ng/ml, in g2 269.1 ± 125 ng/ml, and in g3 732.7 ± 243 ng/ml (p < 0.001). Disease activity score (DAS)28, C-reactive protein, and erythrocyte sedimentation rate were comparable in the three groups at baseline. Disease duration in g3 was the longest (median(IQR) years: g1 = 11(5–15), g2 = 12(8–20), and g3 23(16–26); p = 0.006), with years of disease evolution being correlated with sIL-6R levels (R = 0.417, p < 0.001). Simple and Clinical Disease Activity Index (SDAI and CDAI) decreased significantly in the three groups. However, EULAR response criteria and remission achievement at 6 m was different in the three groups (p = 0.03 and 0.04, respectively). In all. 17 out of the 18 patients in g1 had a good or moderate response to tocilizumab. Conversely, the percentage of patients with no response to tocilizumab was higher in g3 than in g1 and g2. We also observed different changing patterns of IL-6 and sIL-6R levels among the three groups.ConclusionsRA patients could be easily stratified prior to therapeutic intervention with two molecules related to the pathway blocked by tocilizumab. G1 patients, who had the best response to tocilizumab, had the highest level of IL-6 and the lowest level of sIL-6R.     

急性心肌梗塞患者外周血IL-2、sIL-2R水平及动态变化的研究

本文测定了30例急性心肌梗塞患者外周血白细胞介素2（IL-2）及其游离受体（sIL-2R）水平，并进行动态观察，实验结果表明，发病后48h内IL-2水平最低，而sIL-2R水平最高，与正常对照组比较有显著性差异（P＜0.001）；发病后72hIL-2水平开始升高，sIL-2R水平开始下降，至2～3周时两者水平逐渐趋于正常，与对照组比较无显著性差异（P＞0．05）。研究表明急性心肌梗塞与机体免疫应答密切相关。     

Serum osteocalcin levels in patients with psoriatic arthritis: an extended report

The aim of the study was to investigate the rate of bone formation in patients with psoriatic arthritis (PsA) compared to controls and patients with psoriasis vulgaris without PsA (PS). Osteocalcin (OC) and other parameters of bone turnover were measured in 32 patients with PsA and 17 patients with PS and compared to controls (n= 50). Patients with PsA do not generally present with different OC levels (3.0 ± 1.6 ng/ml), than controls (3.6 ± 1.17 ng/ml), if disease activity or sex are not considered. Women with PsA had significantly lower OC levels (2.28 ± 0.44 ng/ml) than female controls (4.11 ± 1.7 ng/ml) or women with PS (3.0 ± 0.89 ng/ml). However, mean disease activity (2.27 ± 1.0 vs 2.95 ± 0.92) was also significantly lower in women than men. Furthermore, we found a significant correlation between alkaline phosphatase (AP) and OC in all patients with PsA (r=0.49, P < 0.05). Disease activity of PsA had an influence on OC levels. Patients with no disease activity had lower OC levels (2.2 ± 0.7 ng/ml) than patients with a high activity (OC 3.92 ± 1.25, P < 0.05). Similar results were obtained with alkaline phosphatase. In addition, we found a significant correlation between clinical activity and OC (r= 0.38, P < 0.02) and alkaline phosphatase (r=0.49, P < 0.01). Patients with PsA show a corresponding increase in OC levels, if disease activity is high. The proliferative changes in active PsA may be related to inflammatory mechanisms coupled with bone formation. Received: 28 May 1999 / Accepted: 11 February 2000     

小肠移植患者可溶性白介素2受体的早期变化(附一例报道)

小肠移植现为治疗短肠综合征、小肠衰竭及由全肠道外营养引起并发症患者的标准治疗方法。小肠移植在脏器移植中开展较早，但由于小肠移植手术难度大，极易发生免疫排斥反应、感染等一系列并发症，严重制约了临床小肠移植的开展，也使小肠移植成为最具挑战性的腹腔脏器移植。2004年7月14日，我院为一名短肠综合征的患者成功实施了尸源性同种异体小肠移植手术，并对其术中、术后早期可溶性白介素2受体（sIL-2R）的变化进行分析，现报告如下。     

IL-2、IL-6及其受体与多发性骨髓瘤的研究

采用固相放射免疫分析法（ＲＩＡ）和双抗体夹心酶联免疫法（ＥＬＩＳＡ）,对多发性骨髓瘤（ＭＭ）患者进行血清白细胞介素２（ＩＬ－２）（ｓＩＬ－２Ｒ）,白细胞介素６（ＩＬ－６）,白细胞介素８受体（ｓＩＬ－６Ｒ）检测,并进行长期随访。结果ＭＭ患者血清ＩＬ０２、ｓＩＬ－２Ｒ和ＩＬ－６、ｓＨ－６Ｒ水平明显升高,ＩＬ－２与２微球蛋白＊β２－ＭＧ）呈负相关,ＩＬ－６,ｗＩＬ－８Ｒ与β－ＭＧ呈正相关,初诊时血清ｓＩ     

Influence of humanized anti-IL-6R antibody, tocilizumab on the activity of soluble gp130, natural inhibitor of IL-6 signaling

Tocilizumab, humanized anti-IL-6R antibody is a novel anti-rheumatic drug. In the present study, we examined the influence of tocilizumab on the inhibitory activity of soluble gp130 (sgp130) against IL-6 signaling. BAF-h130 cells, human gp130 transfected mouse pro-B cell line, were cultured with the mixture with IL-6, sIL-6R and sgp130 for 72 h. BAF-h130 cells proliferated by the addition of both IL-6 and sIL-6R, but not IL-6 or sIL-6R alone. The proliferation induced by IL-6/sIL-6R complex was inhibited by sgp130 concentration-dependently. Tocilizumab inhibited IL-6/sIL-6R-induced cell proliferation. On the other hand, it did not affect the suppressive property of sgp130. To examine if tocilizumab can dissociate IL-6/sIL-6R/sgp130 complex, we established an ELISA system to detect IL-6/sIL-6R/sgp130 complex using anti-IL-6R coated ELISA plate. The results clearly indicated that ELISA system established was detectable IL-6/sIL-6R/sgp130 complex in a concentration dependent manner. Tocilizumab was added to IL-6/sIL-6R/sgp130 complex-fixed plate and then remaining IL-6/sIL-6R/sgp130 complexes on the plate were measured. The amount of IL-6/sIL-6R/sgp130 complexes on the plate was not changed by the addition of tocilizumab. In conclusion, tocilizumab exerts its inhibitory effect through the inhibition of IL-6R directly without affecting natural inhibitor sgp130.     

COPD患者CRP、IL-6、IL-10水平与心血管事件的相关性

目的探讨COPD患者CRP、IL-6、IL-10水平与其心血管事件的相关性。方法选择2013年5月至2014年2月我院收治的60例伴有心血管疾病的COPD老年患者作为观察组,并选择同期60例无合并心血管疾病的COPD老年患者作为对照组。通过放免法检测两组患者体内CRP、IL-6、IL-10水平观察其与患者心血管事件的相关性。结果观察组CRP、IL-6分别为(16.4±2.6)ng/L和(12.8±0.6)mg/L,显著高于对照组,差异具有统计学意义(P0.05);观察组IL-10水平为(8.4±1.2)ng/L,显著低于对照组,差异具有统计学意义(P0.05);随着病情程度加重,CRP、IL-6水平逐渐增加,重度患者含量明显高于轻度患者(P0.05);IL-10水平随着程度加重而降低,重度患者明显低于轻度(P0.05)。结论合并心血管疾病的COPD患者体内炎症反应明显,炎症因子CRP、IL-6升高,抗炎因子IL-10降低,因此检测细胞因子水平在一定程度上可反映病情程度。     

Abnormal T-cell function in patients with psoriatic arthritis: evidence for decreased interleukin 2 production

Summary Psoriatic arthritis (PSA) is an inflammatory arthritis associated with psoriasis. Although not considered an autoimmune process, there is evidence for humoral and cellular immune abnormalities similar to autoimmune diseases such as rheumatoid arthritis and systemic lupus (SLE). We investigated mitogen-induced proliferation and interleukin 2 (IL-2) production by peripheral blood mononuclear cells in patients with PSA. Both IL-2 production and proliferation were significantly decreased in PSA patients when compared to controls. Increased arachidonic acid metabolism has been reported in skin and peripheral mononuclear cells of patients with psoriasis and PSA. We therefore also investigated the effect of indomethacin and prostaglandin E₂ (PGE₂) on IL-2 production. Addition of indomethacin to cultures did not significantly change IL-2 production in patients with PSA, but did so in controls. PGE₂ produced a significant reduction in IL-2 production in PSA and in controls.     

白细胞介素-6受体基因单核苷酸多态性及单体型与2型糖尿病的相关性研究

目的 探讨白细胞介素-6受体(IL-6R)基因单核苷酸多态性(SNP)及单体型与2型糖尿病的相关性.方法 选择入住本院的88例2型糖尿病(T2DM)患者为2型糖尿病组,以同期体检并糖耐量正常的98例健康自愿者为对照组,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)方法对IL-6R基因的2个SNP位点予以基因型检测.采用聚类分析方法分析基因多态性特征.结果 2型糖尿病组D358A基因型中A等位基因频率较对照组高,C等位基因频率较对照组降低,差异有统计学意义(P＜0.05).2型糖尿病组-183(G→A)基因型频率与等位基因频率和对照组比较,差异均无统计学意义(P＞0.05).A2-G单体型在两组中最为常见,其频率分布为0.391、0.288(P =0.053).在4种单体型中两组仅C-A1单体型的频率差异有统计学意义,分别为0.103、0.281 (P =0.001).结论 IL-6R基因第9外显子D358A(rs8192284)是种有价值的遗传性标志,C等位基因对2型糖尿病具有保护作用,携带C-A2单体型的人群较易患2型糖尿病.     

Joint and nail involvement in Turkish patients with psoriatic arthritis

Psoriatic arthritis is an autoimmune, chronic, systemic inflammatory disorder characterized by the association of arthritis with psoriasis. In this paper, we explore the characteristics of joint and nail involvement in Turkish patients with psoriatic arthritis. Forty patients with psoriasis (M/F, 18/22) and 49 (M/F, 25/24) subjects with psoriatic arthritis were included in the study. Clinical characteristics of the patients were recorded. The distribution of the subjects with arthritis: (according to the clinical and radiological findings): polyarticular, 65%; oligoarticular, 23%; isolated axial involvement, 7.7%; arthritis mutilans, 3.8%; sacroiliitis, 19%. Nail involvement was significantly higher among patients with arthritis; i.e., 91 versus 32%; (P < 0.05). There were no correlation between the skin involvement pattern and the arthritis type (P > 0.05). Nevertheless, no relation was observed between the psoriasis duration and arthritis (P > 0.05). Nail involvement is a frequent feature of the psoriatic arthritis which may be a useful finding for differential diagnosis of psoriatic arthritis from other inflammatory arthropathies.     

肿瘤患者治疗前后外周血sIL—2R水平测定

目的：探讨恶性肿瘤患治疗前后细胞免疫功能，方法：用双抗体夹心ＥＬＩＳＡ法检测４０例各类恶性肿瘤治疗前后血清ｓＩＬ－２Ｒ水平。结果：恶性肿瘤患血清ｓＩＬ－２Ｒ水平为１５８５．９１±７５８。１５，显高于正常人２３９．２２±８１．１３（Ｐ〈０．００１），经治疗６个月后（手术，放疗）各类肿瘤患血清ｓＩＬ－２Ｒ较治疗前明显降低，值为６３６．２５±５７６．２５（Ｐ〈０．０５），但仍未降至正常水平。结果     

Gender specific differences in patients with psoriatic arthritis

Objectives: To assess gender related differences in a cohort of patients with psoriatic arthritis (PsA).

Methods: Consecutively recruited patients were included and underwent clinical, radiological and laboratory evaluation by using standardized protocol and case report forms.

Results: Women (n?=?115) with PsA had higher symptom duration and body mass index (BMI), tender and swollen joint counts, disease activity score-28 joints (DAS28), Erythrocyte sedimentation rate (ESR) and poorer physical activity and fatigue than men (n?=?72) with PsA. Psoriasis area and severity index (PASI) were higher in male patients. However quality of life (SF36 physical and mental component scores), articular pattern, extra-articular features (including uveitis, iritis) and family history for psoriasis, spondyloarthritis (SpA) (PsA and ankylosing spondylitis [AS]) were quite similar between men and women.

Conclusions: Some of the clinical and laboratory variables tend to be different between men and women with PsA. The extent of quality of life and articular pattern seem to be similar in both genders. Men with PsA are more likely to have higher PASI scores and longer duration to develop arthritis after the onset of psoriasis, while women are more likely to have higher disease activity and report more fatigue and physical activity limitations.     

Normal levels and function of endothelial progenitor cells in patients with psoriatic arthritis

Endothelial progenitor cells (EPCs) are a population of bone marrow derived cells which have been attributed with the ability to migrate into areas of tissue ischemia and to posses reparative qualities. EPCs have been shown to be decreased in level and function in various inflammatory disorders. Psoriasis and psoriatic arthritis are associated with an increase in cardiovascular morbidity. The aim of the study was to investigate the number of EPCs among patients suffering from psoriasis and psoriatic arthritis. Patients suffering from active psoriasis and psoriatic arthritis were recruited as well as healthy controls. Disease activity was assessed with the DAS-28, BASDAI and PASI scores. Peripheral blood mononuclear cells were isolated and EPC numbers evaluated by FACS analysis using the CD34/133 and CD34/KDR. No significant difference was found between numbers of EPCs between healthy controls, patients with psoriasis and psoriatic arthritis. A significant correlation was found between levels of VGEF and the BASDAI score. The results of the current study do not support a significant role for EPCs in the pathogenesis of psoriasis and psoriatic arthritis.     

GP130 and c-kit signalling, initiated by the sIL-6R/IL-6 complex, is insufficient to expand the primitive adult bone marrow CD34+CD38- pre-CFU cell

 It has previously been shown that gp130 and c-kit signalling synergize for the ex vivo expansion of human cord blood (CB) CD34+ haematopoietic progenitor cells. We were interested in evaluating this synergy within an ontogenetically different haematopoietic tissue [i.e. adult bone marrow (BM)] and on a more primitive progenitor subset (i.e. CD34+CD38– cells), which are highly enriched for pre-colony forming unit (CFU) cells. These cells were plated out in a primary liquid culture supplemented with either interleukin (IL)-6+stem cell factor (SCF), IL-6+SCF+soluble IL-6 receptor (sIL-6R), IL-6+SCF+sIL-6R+IL-3+IL-1 or SCF+IL-3+IL-6+IL-1. Cell counting after liquid culture revealed an absolute expansion of 2.2-, 4.1-, 89.5- and 65.7-fold compared with initial cell input for the four-cytokine combinations, respectively. The secondary read-out assay revealed that this cell expansion in the liquid culture also resulted in CFU generation, with absolute cloning efficiencies of 0.002, 0.024, 12.13 and 7.73 (per cell initially present) for the respective cytokine combinations. These results indicate that gp130 and c-kit signalling alone (i.e. using IL-6+SCF+sIL-6R), in terms of both cell number and CFU generation, insufficiently stimulate primitive adult BM CD34+CD38– haematopoietic cells in order to reach a CFU generation comparable with that obtained after multifactor stimulation. Adding sIL-6R to the multifactor stimulation and compared with this multifactor stimulation, a 1.7-fold synergy in terms of cell expansion and a 3.0-fold synergy in terms of CFU generation are obtained. The sIL-6R/IL-6 complex thus has a narrower spectrum of action on primitive adult BM CD34+CD38– cells than on CB CD34+ cells. Received: 3 January 2000 / Accepted: 20 April 2000     

大承气冲剂对腹内感染致肺损伤患者BLAF中IL-6、IL-10浓度的影响

目的观察大承气冲剂对腹内感染（IAI）致急性肺损伤（ALI）患者局部免疫炎性因子IL-6、IL-10浓度的影响。方法将40例IAI合并ALI的患者随机分为对照组20例和中药组20例;对照组给予西医常规治疗＋安慰剂,中药组给予西医常规治疗＋大承气冲剂,疗程为5 d。记录患者排气、排便时间,并于治疗第1、3、5天分别测氧合指数（OI）及BLAF中IL-6、IL-10浓度。结果中药组排气、排便时间明显短于对照组（P均〈0.05）;中药组OI值较对照组明显升高（P〈0.05）;中药组BLAF中IL-6、IL-10浓度较对照组明显降低（P均〈0.05）。结论大承气冲剂可减轻IAI合并ALI患者局部炎症反应,促进免疫系统及肺肠功能的恢复。     

低氧条件下 IL-6、IL-8、IL-10作用机制研究进展

低氧(hypoxia)是机体循环血液中氧分压较低的状态,缺氧则引发机体的一系列反应。随着研究的深入,发现机体内不但存在氧化应激反应,还伴随着一系列炎症反应,IL-6、IL-8、IL-10作为常见的炎症因子也成为研究热点,本文就低氧条件下三种 IL 的作用机制作一总结,为低氧造成的机体损伤提供更多理论依据,为低氧损伤的预防及治疗提供新靶点。     

非酒精性肝脏疾病IL-6、IL-10测定及其临床意义

目的探讨非酒精性肝脏疾病（NAFLD）患者血清白介素-6（IL-6）、白介素-10（IL-10）的水平及其脂代谢以及胰岛素抵抗的情况.方法检测NAFLD组90例和正常对照组18例的血清IL-6、IL-10、丙氨酸氨基转移酶（ALT）、血脂、空腹胰岛素（FINS）和2 h胰岛素（2HINS）、游离脂肪酸（FFA）,采用稳定模式评价胰岛素抵抗（HOMA-IR）,并通过CT定量评价两组对象肝脏脂肪含量（HFC）.结果NAFLD组IL-6、FFA、ALT水平高于对照组,两组比较有显著差异（P＜0.05）.NAFLD组与对照组比较IL-10水平低于正常对照组（P＜0.05）.NAFLD组与对照组比较腰臀比（WHR）、胆固醇（TC）、甘油三酯（TG）、FINS、2HINS和HOMA-IR、HFC水平均明显高于正常对照组（P＜0.001）.多元逐步回归分析显示WHR和ALT与HFC呈正相关.结论IL-6和IL-10在NAFLD的致病机理中有一定的临床价值.NAFLD患者与胰岛素抵抗密切相关,WHR和ALT是影响NAFLD患者HFC独立的危险因素.