Heavy Metals in Tissues of Blue Crabs Callinectes danae from a Subtropical Protected Estuary Influenced by Mining Residues

Bulletin of Environmental Contamination and Toxicology - This short note aims to report in detail a preliminary assessment of the concentrations of Cd, Hg and Pb in tissues of blue crabs...     

Metals in Horseshoe Crabs from Delaware Bay

We examined the concentrations of arsenic, cadmium, chromium, lead, manganese, mercury, and selenium in the eggs, leg muscle, and apodeme (carapace musculature) in horseshoe crabs (Limulus polyphemus) from eight places on the New Jersey and Delaware sides of Delaware Bay to determine whether there were locational differences. Although there were locational differences, the differences were not great. Further, contaminant levels were generally low. The levels of contaminants found in horseshoe crabs were well below those known to cause adverse effects in the crabs themselves or in organisms that consume them or their eggs. Contaminant levels have generally declined in the eggs of horseshoe crabs from 1993 to 2001, suggesting that contaminants are not likely to be a problem for secondary consumers or a cause of their decline. Received: 25 January 2002/Accepted: 2 June 2002     

Metal Concentrations in Blue Crab (Callinectes sapidus) and Mullet (Mugil cephalus) in Iskenderun Bay, Northern East Mediterranean, Turkey

No abstract available.     

Shell disease and metal content of blue crabs,Callinectes sapidus,from the Albemarle-Pamlico Estuarine System,North Carolina

Concentrations of 13 elements were determined for three tissues (gill, hepatopancreas, muscle) in diseased crabs from a contaminated estuary (Pamlico River, NC), and in non-diseased crabs from both the contaminated estuary and a relatively uncontaminated area (Albemarle Sound, NC) during the fall 1989 and summer 1990. The diseased crabs had lesions which completely penetrated their dorsal integument, while the non-diseased crabs lacked lesions.Sediments within the contaminated area showed enrichment of arsenic, cadmium, manganese, titanium and vanadium relative to the uncontaminated area. Levels of aluminum, arsenic, cobalt, manganese, nickel, titanium, vanadium and zinc were significantly higher in both gill and hepatopancreas in crabs from the contaminated area. Manganese was always highest in the diseased crabs in all tissues measured. The concentrations of the remaining elements were greater in the gills of diseased crabs, while highest values of these elements in the hepatopancreas varied among the diseased and non-diseased crabs from the polluted area. Conversely, copper levels were always highest in all tissues in crabs from the uncontaminated area, and typically lowest in the diseased crabs. Concentrations of aluminum and arsenic were also significantly greater in the muscle tissue of crabs from the contaminated area, but no distinct trend was evident with regard to diseased versus non-diseased crabs.Arsenic was the only element accumulated by crabs in the contaminated area which has a known toxic affect on the tissue responsible for cuticle synthesis and repair (hypodermis) in crustaceans. Metals also accumulated could possibly act synergetically to compromise normal metabolism. The results suggest that metal and trace element accumulation plays a minor direct role in the local etiology of shell disease.     

Relating Disparity in Competitive Foraging Behavior Between Two Populations of Fiddler Crabs to the Subcellular Partitioning of Metals

Behavioral changes in aquatic organisms such as reduced prey capture and decreased mobility have been linked to exposure to contaminants in the field. The purpose of this study was to compare competitive foraging and dominance behaviors of two populations of the fiddler crab, Uca pugnax, and to examine the relationship between tissue metal residues and observed differences in behavior. Foraging behavior (number of total scoops and scoops on a protein-rich patch) and dominance behavior (percentage of successful attacks) of fiddler crabs from an impacted site (Meredith Creek, New York) and a reference site (Tuckerton, New Jersey) were compared in the laboratory. Tuckerton (Tk) crabs were found to have twice the number of total scoops (70 vs. 38 scoops, p < 0.05) and three times the number of scoops on patch (34 vs. 10 scoops, p < 0.05) than Meredith (Me) crabs. No difference was observed between crab populations in the number of successful attacks (i.e., fights over the protein-rich patch). Analyses of total metal body burdens and metals associated with various subcellular fractions showed that, when compared with Tk crabs, Me crabs had higher levels of Ag, Cd, Cu, Ni, and Se in the heat-denatured proteins (HdeP) (i.e., enzymes). Metal bioaccumulation can have inhibitory effects on enzymes, which play an essential role in the regulation of various biochemical, metabolic, and physiological activities in crustaceans. This study suggests that there is a relationship between the accumulation of metals in HdeP (i.e., enzymes) and impairment of competitive foraging behavior in fiddler crabs. Additionally, this study shows that when compared with dominance behavior, foraging behavior is a more sensitive indicator of metal exposure and might be used as an end point in ecotoxicology studies.     

The presence and antimicrobial susceptibilities of human-pathogen Vibrio spp. isolated from blue crab (Callinectes sapidus) in Belek tourism coast,Turkey

Monitoring of Vibrio species by blue crabs (Callinectes sapidus) was carried out during the winter period in a selected area of the Belek, Antalya Gulf. Eighty-three blue crabs were examined for Vibrio species. V. alginolyticus (30.1%), V. fluvialis (10.8%), V. damsela (9.6%), V. harveyi (3.6%), V. metschnikovii (3.6%) and V. vulnificus (2.4%) were isolated. V. vulnificus was the highest concentration (5 x 10(8) Vibrio ml(-1)) although it was only 2.4% isolated from blue crabs. The strains of different vibrio species were highly susceptible to doxycycline, tetracycline and ciprofloxacin.     

Concentrations of Heavy Metals in American Woodcock Harvested in Connecticut

The American woodcock is an important migratory species to both consumptive and nonconsumptive users throughout the species range. Woodcock populations have been declining steadily since surveys began in 1968. Laboratory and field studies have demonstrated several adverse effects associated with metals in other species, which has led to their inclusion as potential contributing factors to the woodcock population decline. We investigated the concentrations of arsenic (As), cadmium (Cd), chromium (Cr), mercury (Hg), lead (Pb), and selenium (Se) in pectoral muscle (n = 100), livers (n = 108), and kidneys (n = 104) and Pb in wing bones (n = 53) from woodcock harvested in Connecticut. Cd concentrations were elevated in 99% of woodcock livers and kidneys; 30% of kidneys were above 100 μg/g dry weight, a Cd level of reported adverse tissue and reproductive effects in other species. Hg and Cr concentrations were below current biologically significant thresholds. Asc, Pb, and Se in soft tissues were associated with lead shot use in collection. Se in 24% of livers was above 10 μg/g dry weight, the reported threshold for avian health. Wing-bone Pb was elevated above 20 μg/g in 61% of adults and 23% of juveniles examined. Our findings indicate that woodcock are being exposed to potentially damaging levels of at least two metals with known effects in other species.     

Assessment of Metal Concentrations in Muscles of the Blue Crab, Callinectes danae S., from the Santos Estuarine System

This study determined the concentrations of eleven metals in the blue crab, Callinectes danae, from nine sites in the Santos Estuarine System of Sao Paulo State, Brazil. The results were compared to guidelines established in the United States, Europe and Brazil for the safety of human consumers. Muscles of blue crabs were removed by dissection and concentrations of Al, Cd, Co, Cr, Cu, Fe, Hg, Mn Ni, Pb and Zn were determined. In general, the concentrations of metals were low, and the crabs were regarded as safe for human consumption. Crabs from a single site (site 4) exceeded the guidelines established by the United States and Europe, but not Brazil, for Pb, with a mean tissue concentration of 1.725?μg?g(-1). With the exception of Al, Fe and Ni, significant differences were noted between sites in the concentrations of each metal in crab tissue.     

Heavy Metals in the Blue Crab (<Emphasis Type="Italic">Callinectes sapidus</Emphasis>) in Mersin Bay,Turkey

The concentrations of four metals (copper, cadmium, zinc and lead) were quantified in blue crabs (Callinectes sapidus) tissues collected in January, April, June and September at two stations in Mersin Bay, northeastern Mediterranean Sea, using ICP-MS. The metal concentrations in crabs from the two stations differed significantly. The hepatopancreas was the major organ accumulating metals, followed by gill and muscle tissues. Except for lead, the highest concentrations of metals were measured in the hepatopancreas. The hepatopancreas had higher concentrations of all heavy metals (Cu 321.1?±?4.30; Zn 182.2?±?3.40; Cd 48.2?±?2.00) compared to gill (Cu 90.2?±?1.35; Zn 104.3?±?2.30; Cd 22.3?±?3.40) and muscle (Cu 19.1?±?1.10; Zn 55.1?±?3.25; Cd 2.5?±?0.20). Among the metals analyzed, Cu, Zn and Pb were the most abundant in the different tissues while Cd was the least abundant in C. sapidus. Seasonality in the levels of the four metals was determined.The highest concentrations of all metals were observed in the June (Zn 55.1?±?3.25; Cu 19.1?±?1.10; Cd 2.5?±?0.20; Pb 5.1?±?0.18) compared to all other months (Zn 34.1?±?3.23; Cu 11.1?±?1.15; Cd 0.9?±?0.20; Pb 3.1?±?0.21).     

Effects of the anionic surfactant, sodium dodecyl sulfate, on newly-hatched blue crabs, Callinectes sapidus, and other routinely tested estuarine crustaceans

This study describes the use of newly hatched larvae of Callinectes sapidus (blue crab) in a 48-h acute toxicity test and compares their sensitivity to two other estuarine crustaceans (Mysidopsis bahia and Palaemonetes pugio) commonly used for evaluation of effects of potentially toxic materials. C. sapidus larvae were twice as sensitive to sodium dodecyl sulfate as 24-h post-release M. bahia, and five times more sensitive than 24-h-old P. pugio larvae. We found the blue crab toxicity test to be simple, rapid and accurate and it provides low variability and high reproducibility. Since the data indicate high sensitivity of this commercially important species to a reference toxicant and the potential impact on its survival during a critically sensitive developmental stage, we propose future research further evaluating C. sapidus as a potential toxicity test species.Contribution No. 943 of the U.S. Environmental Protection Agency, Health Environmental Effects Research Laboratory, Gulf Ecology Devision, Gulf Breeze, FL     

Accumulation of Trace Metals in Two Commercially Important Shrimp Species from Camamu Bay, Northeastern Brazil

Camamu Bay is the second largest estuary in Bahia state, northeastern Brazil, being recognized by its high diversity and economical relevance for fisheries and tourism. To evaluate the impacts of environmental disturbances in Camamu Bay, trace metals (Cr, Mn, Fe, Co, Ni, Cu, and Pb) were quantified in two widespread and commercially exploited shrimp species (Farfantepenaeus paulensis and Xiphopenaeus kroyeri). High concentrations of all metals but Pb were observed in both species by ICPOES. The concentration values for Cr, Co, and Mn were invariably higher than the accepted limits for human consumption in Brazil. Inter and intraspecific variation in metal levels might be related to biological particularities and body size. The accentuated contamination by trace metals in both species validated them as efficient bioindicators of environmental quality. Thus, effective planning, monitoring and regulatory policies should be adopted to inspect and remediate the metal contamination in natural resources from Camamu Bay.