脱蛋白骨-钛网支架成骨细胞复合物修复骨缺损的研究

探讨成骨细胞和脱蛋白骨-钛网(DPB- TM)支架复合物修复骨缺损的能力。用新西兰兔30只,随机分成3组,各10只。实验组第三代胎兔成骨细胞以5×10 6 m l- 1浓度接种于DBP- TM支架中,复合物体外培养1周后修复颅骨1.5 cm×1.5 cm缺损。对照组 、,同样的缺损分别用DBP- TM支架修复和注射成骨细胞悬液。术后12周,三维CT检查后,对取下的标本进行大体、扫描电镜、组织学观察及生物力学测试。结果显示,实验组植入的复合物与正常骨组织之间分界线模糊,有骨小梁通过,界面紧密融合;支架上有大量新骨形成,且支架部分被吸收;植入12周后的复合体抗压强度(18.93±1.12 MPa)大于正常颅骨(16 .96±1.6 0 MPa) (P<0 .0 5 )。对照组均无新生骨,仅见缺损区有纤维样组织覆盖。因此,体外培养的成骨细胞经增殖后接种于DPB- TM支架中可以用来修复骨缺损。     

SD大鼠骨髓基质细胞向神经干细胞诱导分化的初步实验研究

以 SD大鼠为实验对象 ,从其骨髓分离出骨髓基质细胞 (BMSC) ,利用 L IF、b FGF、RA等增殖及分化诱导因子和神经干细胞培养液进行培养、分化诱导 ,观察 SD大鼠骨髓基质细胞体外培养的生长行为及扩增、分化情况。我们观察到 ,分离得到的骨髓基质细胞在体外培养中能形成细胞克隆团 ,这些具有克隆能力的骨髓基质细胞经传代后 ,其数量明显多于原代培养 ;BMSC经持续培养具有增殖能力以及分化为组织细胞的潜能 ,其分化细胞形态多样 ,包括胶质细胞样细胞和神经元样细胞。实验结果证明 ,骨髓基质细胞具有较强的自我更新的能力和多分化能力 ,也说明本实验所采用的细胞培养方法适用于骨髓基质细胞的体外生存与扩增。骨髓基质细胞较容易取材、体外培养和扩增 ,并具有多向分化潜能 ,在合适的诱导分化条件下 ,可分化出神经细胞 (神经元和神经胶质细胞 ) ,是理想的种子细胞     

Graft Transit Time Has No Effect on Outcome of Unrelated Donor Hematopoietic Cell Transplants Performed in Australia and New Zealand: A Study from the Australasian Bone Marrow Transplant Recipient Registry

A previous study found that platelet recovery and mortality were worse in recipients of myeloablative bone marrow transplants where graft transit times were longer than 20 hours. This retrospective study of unrelated myeloablative allogeneic transplantation performed within Australia and New Zealand analyzed transplant outcomes according to graft transit times. Of 233 assessable cases, 76 grafts (33%) were sourced from bone marrow (BM) and 157 (67%) from peripheral blood. Grafts sourced from Australia and New Zealand (47% of total) were associated with a median transit time of 6 hours versus 32 hours for overseas sourced grafts (53% of total). Graft transit temperature was refrigerated in 85%, ambient in 6%, and unknown in 9% of cases, respectively. Graft transit times had no significant effect on neutrophil or platelet engraftment, treatment-related mortality, overall survival, and incidence of acute or chronic graft-versus-host disease. Separate analysis of BM grafts, although of reduced power, also showed no significant difference in either neutrophil or platelet engraftment or survival between short and longer transport times. This study gives reassurance that both peripheral blood stem cell and especially BM grafts subjected to long transit times and transported at refrigerated temperatures may not be associated with adverse recipient outcomes.