Use of ultraviolet irradiation of the blood in the complex treatment of peritonitis

According to the data of tetrasolium nitroblue test and titer of the blood serum antibodies to the most frequently cultured microflora in complex treatment of 27 patients with diffuse purulent peritonitis, it was established, that the use of ultraviolet irradiation of the blood stimulated the specific and non-specific organism resistance.     

The effect of ultraviolet irradiation of the blood on its oxygen transport function

In 28 patients with purulent septic diseases and in bench test of the donor conserved blood,it was established that ultraviolet irradiation was followed by improvement in the oxygen transport function of the blood, resulting from the formation of peroxide oxygen compounds and activation of carbohydrate metabolism.     

Modern methods of treatment of diabetic foot syndrome]

Method of complex treatment of the diabetic foot syndrome (DFS), which included combined application of intravascular laser irradiation of blood, ultraviolet irradiation of blood, indirect electrochemical detoxication of organism, endolymphatic therapy, abacterial environment, modern methods of physical antisepsis was elaborated and introduced in practice.     

The use of ultraviolet-irradiated plasma in the treatment of peritonitis

Intravenous infusion of the one-group lyophilized donor plasma irradiated with ultraviolet rays was included in the complex treatment of 43 patients with acute purulent peritonitis. The species-specific composition of the microflora was studied in the different groups of patients according to the cause of peritonitis and the degree of microbial contamination of the purulent wounds in patients treated with and without ultraviolet plasma irradiation (UVPI). For prognosticating the course and outcome of the disease the authors used a complex of indices linked with the manifestation of neutrophil bactericidal activity and the level of exogenous intoxication. A positive effect of UVPI in the management of acute purulent appendicitis was noted, which was confirmed by clinical and microbiological tests.     

Helium-neon lasers in the treatment of suppurative-inflammatory diseases in children

The use of radiation of the helium-neon laser makes time of the treatment of patients with purulent processes 4-6 days shorter, prevents the formation of rough scars and deformities at the place of the former purulent wound. The laser irradiation is especially effective when combined with proteolytic enzymes.     

Cryoprocedures in the treatment of suppurative wounds

An analysis of results of treatment of 135 patients with purulent wounds and acute pyonecrotic injuries of soft tissues has shown the application of cryoprocedures especially when combined with the laser irradiation to promote earlier cleaning of the wound from necrotic tissues, early appearance of granulations, quicker healing the wounds and medical rehabilitation of the patients.     

Mechanism of laser ablation in an absorbing fluid field

Selection of a laser source for intravascular applications has frequently been predicated upon assumptions involving transmissibility in blood of the wavelength of light emitted by a given laser. Standard absorption curves for ultraviolet radiation in blood and infrared radiation in water would suggest that transmission of ultraviolet radiation through a blood field and infrared radiation through any aqueous fluid field would be insufficient for tissue ablation. The present series of experiments was undertaken to determine whether these theoretical predictions would in fact obviate the use of these wavelengths in a blood field. Specimens of normal human myocardium and/or polyvinylchloride were submerged under blood and water and irradiated with ultraviolet radiation (351 nm) delivered as a focused beam and via an optical fiber and infrared radiation (10,600 nm) delivered as a focused beam. Ablation of myocardium was successfully accomplished with a focused beam of both ultraviolet and infrared radiation under as much as 5 mm of blood and with ultraviolet radiation via an optical fiber with the fiber tip up to 3 mm distant from the tissue specimen. High-speed cine recordings of ablation carried out using a focused beam of laser radiation demonstrated that formation of a dynamic optical cavity is the basis for successful pulsed ultraviolet and infrared laser transanguineous tissue ablation. These results thus demonstrate that prediction of wavelength transmission through fluid media based on optical properties of a static fluid does not predict ability to accomplish ablation under dynamic circumstances of laser irradiation.     

Autotransfusion of ultraviolet-irradiated blood in destructive pneumonia of young children

Analysis of the results of clinicoimmunological study of the use of autotransfusion of blood treated by ultraviolet irradiation (ABUVI) in infants with acute purulent destructive pneumonia (APDP) revealed that imbalance of cellular and humoral immunity factors was the main factor determining the severity of the disease. ABUVI is an effective measure for correcting the immune response of the child's organism to the bacterial aggression through adequate production of monocytic phagocytes and plasma cells of the blood. It also influences the completeness of humoral immunity and reduction of T-lymphocyte deficiency in the acute phase of the disease. ABUVI raises the efficacy of complex treatment of toxicoseptic forms of APDR, reduces 1.7-fold the terms of treatment, and reduces considerably the mortality rate of this disease in young children.     

Metabolic bases of the pathogenesis of severe multiple trauma

Under analysis there were data of hormonal activity of the hypothalamo-hypohysis-adrenocortical system and the incretory apparatus of the pancreas, metabolism (of proteins, lipids, carbohydrates) and concentration of microelements (iron, zinc), lipid peroxidation (content of malonic dialdehyde and diene conjugates, on the one hand, and total oxidative activity of blood, superoxide dismutase (SOD), ceruloplasmin, catalase, SH-group, on the other hand) and results of its therapeutic correction (with superoxide dismutase, ionole, extracorporeal laser irradiation and laser irradiation of the wounds), content of the inflammatory reaction markers (tumor necrosis factor and IL-1) in patients with a severe combined trauma in the dynamics of traumatic disease. The results obtained and literature data allow a conclusion to be made that the basis of metabolic changes in severe combined traumas are formed by a developing adaptation reaction of organism immediately after trauma integrated with the systemic aseptic inflammatory response caused by vast necrosis of tissues, hypo- and reperfusion processes. The intensity of these reactions depends on severity of the trauma, determines its clinical course and outcome. The therapeutic correction of higher activity of the lipid peroxidation system (caused by a systemic inflammatory response) ionol, extracorporeal laser irradiation of blood, laser therapy of the wounds increase the antioxidative volume of blood and decrease the production of lipid peroxidation. Early intrabronchial instillation of homogenic SOD increases the total antioxidative activity, the level of SOD and catalase, decreases the content of malonic dialdehyde in the sediment of broncho-alveolar lavage and in blood.     

Sorption methods and laser irradiation in the treatment of suppurative and septic diseases

The application therapy of purulent wounds with sorbents during the first phase of the wound process reduces osmotic hyperhydration of tissues around the wound, promotes normalization of pH and phagocytosis of the wound exudate, makes the healing of the wounds 32.4% quicker. The combined use of the application therapy and external laser irradiation makes the time of healing almost 2 times shorter. In sepsis hemosorption results in the effective elimination of underoxidized products of free radical oxidation, in normalization of activity of the Kallikrein-Kinin system, improves results of the treatment.     

Low-intensity infrared laser increases plasma proteins and induces oxidative stress in vitro

Low-intensity laser therapy is based on the excitation of endogenous chromophores in biotissues and free-radical generation could be involved in its biological effects. In this work, the effects of the low-intensity infrared laser on plasma protein content and oxidative stress in blood from Wistar rats were studied. Blood samples from Wistar rats were exposed to low-intensity infrared laser in continuous wave and pulsed-emission modes at different fluencies. Plasma protein content and two oxidative stress markers (thiobarbituric acid-reactive species formation and myeloperoxidase activity) were carried out to assess the effects of laser irradiation on blood samples. Low-intensity infrared laser exposure increases plasma protein content, induces lipid peroxidation, and increases myeloperoxidase activity in a dose- and frequency-dependent way in blood samples. The low-intensity infrared laser increases plasma protein content and oxidative stress in blood samples, suggesting that laser therapy protocols should take into account fluencies, frequencies, and wavelengths of the laser before beginning treatment.     

Management of the perineal wound in the postoperative treatment of patients with cancer of the rectum

The material showing causes of the appearance of purulent complications of the perineum would after extirpation of the rectum for cancer is presented. The pathogenes of the purulent infection were found to be polyresistant staphylococcus and gram-negative intestinal flora in most cases. The authors recommend using antibiotic therapy directly at the time of performing the operation, good drainage of the postoperative wound, irradiation of the postoperative wound by a laser radiation of a harmless intensity.     

Efficacy of ultraviolet blood irradiation therapy in the control of staphylococcemias

There has been presented a report and analysis of sixteen cases of staphylococcemia given ultraviolet blood irradiation therapy as a method of controlling this type of acute pyogenic infection.The first seven staphylococcemic individuals treated by ultraviolet blood irradiation therapy failed to respond and died. Six of these seven received intensive sulfa drug therapy, whereas the seventh, whose Staphylococcemia arose from a bladder carcinoma and was complicated by atelectasis and empyema at the time of institution of ultraviolet blood irradiation therapy, received only blood irradiation and also died.The results of ultraviolet brood irradiation therapy in a second group of nine consecutive staphylococcemic individuals given blood irradiation was reported; all nine of these individuals recovered uneventfully; eight of the nine received no suIfa drugs whatsoever, and the ninth, forty-eight hours of sulfathiazole therapy one week before bIood culture became positive.In each of the sixteen cases reported one or more pure cuItures of pathogenic strains of either Staphylococcus aureus or StaphyIococcus albus were obtained from blood cuItures taken in tryptose phosphate broth.In fifteen of the sixteen cases there was present a profound toxemia at the time of institution of uItravioIet bIood irradiation therapy.Uterine diratation and curettage was performed in three individuaIs of Group Two, the recovery group, whiIe the blood culture in each of the three individuals was still positive. No untoward effects were observed to follow this radical procedure performed despite the presence of staphylococci in the bIood stream.     

Hairless mouse epidermal antioxidants and lipid peroxidation assessed by He-Ne laser

BACKGROUND AND OBJECTIVE: To evaluate the possibility of oxygen radical damage in the skin after He-Ne laser irradiation according to dose intensity and time. STUDY DESIGN/MATERIALS AND METHODS: The He-Ne laser (lambda = 632.8, 10 mW) was used on the skin of mice with 1, 3, and 5 joule (J/cm2) dose rates for 1, 5, and 7 days in each case, and the results were compared with normal and anesthetic nonirradiated skins. The efficacy was determined by the formation of thiobarbituric acid-reactive substances (TBARS) in a 10-minute period and expressed as a concentration of malondialdehyde (MDA) from the lipid peroxidation of epidermal tissue, and total superoxide dismutases (SODs) and catalase activities, correlated with histologic biopsies. RESULTS: Data from epidermal SODs, catalase activity and the degree of lipid peroxidation at low-power radiation showed that repeated exposure had led to the induction of free radical damage and of epidermal changes as confirmed by microscopic study. The application of the He-Ne laser at 1, 3, and 5 J intensity for 5 days caused a gradual increase in the SODs and catalase activities, while the levels of TBARS were slightly decreased in the mouse epidermis. However, these patterns were reversed after 3 and 5 J irradiations for 5 and 7 days laser treatment. Furthermore, microscopic examinations revealed that the laser-irradiated skin changed the release of stratum granule from epidermis to hair follicle, and produced blood vessel thrombosis of the dermal capillary plexus. CONCLUSION: The presence of lipid peroxidation in the hairless mouse skin after exposure to He-Ne laser energy intensity of over 3 J for over 5 days was demonstrated. This lipid peroxidation could have been generated from oxygen free radicals. The histologic and oxidative enzymatic correlations on lipid peroxidation in the skin have provided a better understanding of He-Ne laser therapy-tissue interactions. It is possible to take advantage of these findings to evaluate pathologic skin conditions and effective laser dosage more efficiently.     

Lipid peroxidation before and after amputation of the lower extremity in patients with atherosclerotic gangrene

Activity of lipid peroxidation processes in blood was studied in 60 patients with atherosclerotic gangrene of the lower extremity subjected to amputation of the extremity in a prospective controlled investigation. Activation of the lipid peroxidation processes with a lower number and less activity of the antioxidant system enzymes was demonstrated in patients with severe critical ischemia of lower extremities. The operation trauma was followed by further stress of the system of free radical oxidation of lipids. The increased concentration of lipid peroxidation products with a decreased or low level of activity of the antioxidant system enzymes allows prognosis of the development of purulent complications at the early postoperative period.     

The influence of laser and electrophoresis of myramistin on dehydrogenases activity of the blood neutrophils in complex treatment of purulent complications in abdominal surgery

It was established in clinical and experimental investigations that while the purulent postoperative complication occurrence the activity of succinate dehydrogenase, alpha-glycerophosphatedehydrogenase, glucoso-6-phosphatedehydrogenase is reducing and the activity of the blood neutrophils lactatedehydrogenase is rising. Application of a laser and electrophoresis with Miramistin had promoted the normalization of these enzymes activity, substantiating its usage in complex therapy of purulent complications of the operation wound healing.     

Complex correction of intoxication syndrome in sepsis

The experience with treatment of 120 patients with endotoxicosis caused by sepsis of different etiology is summarized. In the complex of treatment, the endolymphatic therapy, application, gastrointestinal sorption, hemosorption, plasmapheresis, plasmosorption, ultraviolet irradiation of the blood were used.     

The thoracoscopic use of the CO2 laser in the combined treatment of pleural empyema]

A new method for treating acute pneumothorax with a bronchopleural fistula was experimentally developed and clinically tested. It is based on thoracoscopic use of CO2 laser radiation. The laser beam energy is employed for evaporation of necrotic tissues in the area of pulmonary destruction, "welding" of the bronchopulmonary fistulae, and general irradiation of the empyema cavity with a defocused beam. Nineteen patients with various disease duration were treated. In 16 patients the bronchial fistulae and the empyema cavity were eliminated and the lung re-expanded. The purulent inflammation in the pleural cavity was acute in these cases.     

Characterization of pulsed-dye laser-mediated vasodilatation in a rabbit femoral artery model of vasoconstriction

Vasoconstriction is a clinical problem associated with invasive vascular procedures, microvascular reconstruction and subarachnoid hemorrhage. We sought to characterize the ability of pulsed-dye laser irradiation to reverse and prevent vasoconstriction in an anesthetized rabbit model of surgically and pharmacologically induced vasoconstriction. Five groups of experiments were performed to study the effect of pulsed-dye laser irradiation delivered through a 320 μm core ball-tip fiber into the femoral artery. The studies demonstrated that pulsed-dye irradiation can reproducibly cause vascular dilatation. The zone of vasodilatation propagated equally proximal and distal to the site of irradiation within the vessel. When saline was infused into the vessel to replace flowing blood during delivery of laser irradiation, no significant vasodilatation occurred. After laser irradiation reversed surgical and pharmacologic vasoconstriction, the vessel was resistant to further pharmacologic Vasoconstriction. This resistance to pharmacologic vasoconstriction did not occur if the vessel was pharmacologically predilated before delivery of laser irradiation. Pathologic analysis of the vessels revealed endothelial damage and mild to moderate medial necrosis, most significant at the site of energy delivery. These studies provide characterization of pulsed-dye laser-mediated vasodilatation in an in vivo model. Delivery of pulsed- dye laser energy has potential clinical application and warrants further investigation. © 1993 Wiley-Liss, Inc.