荧光定量PCR方法检测婴儿尿液中人类巨细胞病毒基因的含量

目的探讨FQ-PCR方法检测婴儿尿液中HCMV-DNA含量对于临床症状性HCMV感染的诊断价值,同时,比较FQ-PCR方法与定性PCR方法的敏感性.方法应用双标探针水解的FQ-PCR方法检测89例临床怀疑有巨细胞病毒感染患儿(病例组)尿液中的HCMV-DNA拷贝数,并以82例同年龄组健康婴儿做对照,比较两组阳性标本中HCMV-DNA含量.同时,在两组中随机选取94例(其中疑诊为HCMV感染的56例,健康儿38例),用FQ-PCR方法与普通定性PCR方法共同检测进行比较.结果病例组阳性52例,称为症状性感染组,对照组阳性30例,称为无症状性感染组.症状性感染组52例,尿液HCMV-DNA拷贝数对数值(log10X)在3.81-9.93,平均为5.30;无症状性感染组30例,尿液中HCMV-DNA拷贝数对数值在2.70-4.60,平均为3.46,两组均值差异有显著性(T=8.09,P＜0.01),拷贝数大于105拷贝/mL高度预示症状性HCMV感染.FQ-PCR方法阳性率为60.64%(57/94),定性PCR方法阳性率32.98%(31/94),两方法一致率70.21%(χ2=25.30>6.63,P＜0.01),FQ-PCR方法优于定性PCR方法(χ2=23.32>6.63,P＜0.01).结论婴儿尿液中HCMV-DNA拷贝数与临床症状性HCMV感染密切相关,FQ-PCR方法检测婴儿尿液中HCMV-DNA量可以作为临床诊断HCMV症状性感染的一种快速、有效的实验室方法.     

婴儿血液、尿液及母乳中病毒DNA检测在诊断人巨细胞病毒感染中的应用

目的 定量检测疑似人巨细胞病毒(HCMV)感染婴儿血液、尿液及对应母亲乳汁中的HCMV-DNA,评估三者在不同年龄组内辅助诊断HCMV感染的意义。方法 选取170例疑似HCMV感染婴儿,根据年龄分为两组:新生儿组(< 28 d, n=43)和28 d~5个月组(n=127),分别收集血液、尿液及母乳,应用荧光定量聚合酶链式反应法(FQ-PCR)检测HCMV-DNA。结果 新生儿组血液、尿液及母乳HCMV-DNA阳性检出率分别为65.1%、18.6%和93.0%,28 d~5个月组三者检出率分别为64.6%、92.9%和72.4%,28 d~5个月组尿液检出率显著高于新生儿组(P<0.01),而母乳检出率却显著低于新生儿组(P<0.01)。82例血液和尿液HCMV-DNA为阳性的患儿,其尿液HCMV-DNA拷贝数明显高于血液。结论 不同年龄组尿液及母乳中HCMV-DNA检出率不同,根据年龄选择合适的送检标本对提高检出率具有重要意义。     

儿童难治性癫(癎)外周血P糖蛋白的表达及药物干预

目的 测定儿童难治性癫(癎)及初诊癫(癎)患儿外周血白细胞多药耐药基因产物P糖蛋白(P-gp),建立儿童难治性癫(癎)耐药性客观指标及预测儿童难治性癫(癎)客观指标;同时观察氟桂利嗪的疗效.方法 应用流式细胞仪检测41例难治性癫(癎)儿童及45例初诊癫(癎)儿童外周血多药耐药基因产物P-gp的表达,44例健康儿为对照组.难治性癫(癎)组患儿加用氟桂利嗪(弗瑞林)2.5～5 mg,qn口服治疗,对其疗效进行临床验证.结果 难治性癫(癎)组P-gp表达阳性23例(56.1%),初诊癫(癎)组P-gP表达阳性10例(22.2%),对照组P-gp表达阳性3例(6.8%),三组比较差异有统计学意义(X2=26.77,P(0.01).难治性癫(癎)组与对照组比较X2=24.27,P<0.01;难治性癫(癎)组与初诊癫痫组比较X2=10.41,P<0.01;初诊癫痫组与对照组比较X2=4.23,P<0.05.难治性癫痫组P-gp表达阳性23例中17例(73.9%)无效,6例(26.1%)有效,P-gP表达阴性18例中3例(16.7%)无效,15例(83.3%)有效,两者比较差异有统计学意义(X2=10.10,P<0.01).初诊癫痫组P-gp表达阳性10例中7例(70.0%)转为难治性癫(癎),P-gp表达阴性35例中3例(18.6%)转为难治性癫(癎).两者比较差异有统计学意义(X2=16.98,P<0.01).难治性癫(癎)组抗癫(癎)药物治疗无效者20例加用氟桂利嗪治疗3个月,P-gP表达阳性17例中有效11例(64.7%),11例有效者复查P-gP有6例(54.5%)转为阴性;无效6例(35.3%),复查P-gP表达仍阳性.P-gP表达阴性3例中1例有效,2例无效,复查P-gp表达阴性.结论 外周血多药耐药基因产物P-gP在儿童难治性癫(癎)中表达增强,可作为儿童难治性癫(癎)耐药性的客观指标;P-gP表达阳性的初诊癫(癎)患儿多转为难治性癫(癎),P-gP可作为预测儿童难治性癫(癎)的客观指标;氟桂利嗪有一定抗癫(癎)及逆转P-gP的表达作用.     

儿童癫痫影像学检查的意义

目的为阐述影像学检查对病因诊断和癫癎分类意义,并就癫癎患儿影像学检查提出合理建议.方法将318例初次被诊断癫癎并接受神经影像学检查(包括CT和MRI)患儿,根据影像学检查结果,分为正常组和异常组.结果影像学异常表现多样;影像学检查结果阳性组平均发病年龄小,肌阵挛性发作多,且多伴发育迟缓、脑性瘫痪,有显著性差异(P＜0.05);所有失神癫癎患儿影像学检查均为阴性.结论影像学检查在癫癎病因方面有重要临床意义,但对儿童失神癫癎患儿可不必行影像学检查.     

动态脑电图、脑干听觉诱发电位在脑性瘫痪预后康复评价中的意义

目的探讨动态脑电图(AEEG)和脑干听觉诱发电位(BAEP)在脑性瘫痪(脑瘫)儿预后康复评价中的作用。方法对128例脑瘫患儿分别在清醒、活动及睡眠状态下检测AEEG及入睡后用10%水合氯醛检测BAEP,并对AEEG、BAEP检测结果进行分析。结果AEEG正常48例(37.5%),异常80例(62.5%)。BAEP正常25例(19.5%).异常103例(80.5%)。并智力低下及癫癎患儿的AEEG异常率高于平均异常率。手足徐动型、混合型脑瘫患儿的BAEP异常率高于平均异常率。绪论脑瘫类型不同,AEEG、BAEP异常率不同。AEEG与BAEP两者从不同的解剖径路发现脑瘫的并发症及判断预后,有助于脑瘫的全面康复。     

难治性癫癎的相关因素分析

目的探讨儿童难治性癫癎(IE)的临床相关因素。方法采用回顾性分析的方法对38例IE患儿(IE组)与40例药物治疗有效的癫癎患儿(对照组)的临床资料(包括起病年龄、发作频率、发作形式、脑电图表现、头颅影像学改变、智能情况、家族史、服药依从性,对首次用药治疗的反应情况等)进行对比分析。结果 IE组1岁前的发病率、发作频率、出现多种发作形式和伴智能障碍率、头颅影像学及脑电图异常率均较对照组增高,差异均有统计学意义(Pa<0.05);IE组首次应用抗癫癎药物治疗反应不良、联合多种药物控制发作及药物治疗依从性差的概率较对照组亦增高,差异均有统计学意义(Pa<0.05)。结论儿童IE与发病年龄小、发作频繁、症状性癫癎或癫癎综合征、多种发作形式共存、伴智能障碍、头颅影像学及脑电图异常改变率高、首次应用抗癫癎药物治疗的反应差、联合多种药物治疗、药物治疗的依从性差等诸多因素有关。     

夜间发作癫癎小儿的临床与动态脑电图特征

目的 探讨夜间发作的各种类型小儿癫癎的临床与动态脑电图特征.方法 收集2005年6月-2006年12月本院小儿神经科门诊夜间发作及非夜间发作的癫癎患儿各38例.记录其性别、年龄、起病年龄、发作频率、癫癎家族史、头颅MRI检查及24h动态脑电图(AEEG)电监测结果.对夜间发作及非夜间发作癫癎的临床特点及脑电图特征进行比较和分析.结果 夜间发作癫癎的临床发作形式包括局限性发作13例(28.1%),局限性继发全面性发作1例(2.6%),全面性发作28例(73.7%).夜间发作癫癎仅于夜间睡眠期癎样放电23例(60.5%),清醒期和睡眠期均有癎样放电且无明显差异9例(23.7%),均有癎样放电但睡眠期明显多于清醒期6例(15.8%).夜间发作与非夜间发作癫癎的临床发作形式、癎样放电部位及异常波主要类型比较无统计学差异(Pа>0.05).二者存在癎样放电的时期有显著性差异(Pа<0.05),且夜间发作癫癎在睡眠期存在癎样放电明显多于非夜间发作癫癎.结论 夜间发作的癫癎形式多样,缺乏特异性临床表现,难以与睡眠障碍相鉴别,睡眠期AEEG对于夜间发作癫癎的诊断和鉴别诊断具有重要意义.     

特发性血小板减少性紫癜并人类巨细胞病毒感染患儿的细胞免疫状态及治疗转归

目的 探讨人类巨细胞病毒(HCMV)感染对儿童特发性血小板减少性紫癜(ITP)的发病、疗效及预后的影响,为合理有效治疗ITP提供参考.方法 收集230例ITP患儿(ITP组)及200例同期急性上呼吸道感染患儿(对照组)比较二组HCMV感染率及细胞免疫状态.按有无合并HCMV感染将ITP患儿分为HCMV感染组88例和非感染组142例,HCMV感染组按治疗方法分为常规治疗组和常规治疗加更昔洛韦抗病毒治疗组.非感染组采用常规治疗.比较各组患儿细胞免疫状态(外周血淋巴细胞亚群比例CD3 CD4 、CD3 CD8 、CD3-CD19 、CD16 CD56 )、近期(2周)治疗效果、远期(6个月)疾病转归之间的关系.采用SPSS 14.0软件进行t检验和x2检验.结果 ITP 230例患儿中,并HCMV感染88例(38.26%),免疫异常165例(71.74%);对照组并HCMV感染22例(11.0%),免疫异常36例(18.0%),二组比较有显著统计学差异(Pa<0.01),二组患儿CD3 CD4 、CD3 CD8 、CD3-CD19 、CD16 CD56 百分比比较均有统计学差异(P<0.05,0.01).初诊时HCMV感染组细胞免疫异常74例(84.09%),非感染组91例(64.08%);6个月后,HCMV感染组检测到细胞免疫异常24例(27.27%),非感染组5例(3.52%),二组比较有显著统计学差异(P<0.01).HCMV感染组与非感染组近期治疗效果无统计学差异(P0.05),HCMV感染组35例(39.78%)病程超过6个月.非感染组24例(16.90%)病程超过6个月,二组比较有显著性差异(P<0.05).抗HCMV治疗组病程超过6个月15例(27.78%),常规治疗组病程超过6个月20例(58.82%),二组比较有显著性差异(P<0.05).结论 HCMV感染诱发的细胞免疫异常是儿童ITP发病及病程迁延的重要因素.抗HCMV治疗对ITP治疗有明确效果,且不良反应少,患儿耐受性好.     

儿童癫癎的诊断和治疗

目的研究癫癎诊断和治疗的现状。方法采用回顾性分析方法,对5年间450例门诊和住院的癫癎患儿资料进行发作类型与年龄的关系、脑电图及药物的疗效分析。结果450例中全面性发作63.1%(284例),其中大发作33.3%(150例);局限性发作16.7%(75例);其他类型发作20.2%(91例)。本组68.2%为6岁以下儿童,其中<3岁占73%。1岁以内发作类型最常见为婴儿痉挛(WS),占该年龄组的41.1%(46/112例)。失神发作见于学龄期儿童,占本组病例的2.4%,Lennox-Gastaut综合征在9岁前各年龄组均可发生,热性惊厥转变为大发作见于学龄期儿童。癫癎发作的诱因主要为发热,有家族史占各种诱因的4.7%。癫癎的病程最长13年。脑电图(EEG)异常率达74.9%,其中普通EEG示癎样放电为50.5%,加做video-EEG或24h动态EEG后阳性率达62.8%。65.7%无影像学异常改变。120例(26.7%)诊断后未治疗或服药后未复诊。330例中单药治疗组总疗效76.5%,联合用药组为55.6%,两者之间疗效存在显著差异(P<0.05),而单药治疗组与WS组疗效比较无显著性差异(P>0.05)。结论癫癎治疗以单药治疗为主,但WS联合用药效果较佳。癫癎治疗滥用药现象较严重,有待规范化治疗。     

不同发作类型癫(癎)患儿血清抗心磷脂抗体变化及临床意义

目的 探讨不同发作类型癫(癎)患儿血清抗心磷脂抗体水平的变化及其临床意义.方法 采用ELISA法检测84例癫(癎)患儿治疗前后及38例正常儿童血清抗心磷脂抗体水平.结果 健康对照组、部分发作组、全身发作组及分类不明发作组血清抗心磷脂抗体阳性率分别为6.3%(2/32)、28.6%(8/28)、33.3%(12/36)、30.0%(6/20),各组比较差异有显著性(X2=9.98,P<0.05);部分发作组、全身发作组及分类不明发作组血清抗心磷脂抗体阳性率均明显高于健康对照组(P<0.01),癫(癎)不同发作类型组间比较差异无显著性(P>0.05).部分发作组、全身发作组及分类不明发作组癫(癎)患儿在治疗后血清抗心磷脂抗体阳性率明显降低,差异有显著性(X2=5.39,P<0.05;X2=14.40,P<0.01;X2=11.67,P<0.01).结论 癫(癎)患儿血清抗心磷脂抗体水平增加,提示癫(癎)发作中可能存在自身免疫异常;治疗后血清抗心磷脂抗体水平降低,为癫(癎)的有效防治提供重要依据.     

母乳喂养对人巨细胞病毒感染婴儿免疫功能的影响

目的 探究母乳喂养对人巨细胞病毒(human cytomegalovirus,HCMV)感染患儿免疫功能的影响。方法 回顾性分析2021年1月—2022年5月郑州大学附属儿童医院收治的135例喂养方式为母乳喂养的HCMV感染患儿的病例资料,根据母乳HCMV-DNA检测结果分为母乳阳性组(78例)和母乳阴性组(57例),再根据母乳HCMV-DNA载量中位数,将母乳阳性组患儿分为母乳高病毒载量亚组(39例)和母乳低病毒载量亚组(39例),分别比较母乳阳性组和母乳阴性组、母乳高病毒载量亚组和母乳低病毒载量亚组患儿外周血淋巴细胞亚群CD3⁺T、CD3⁺CD4⁺T、CD3⁺CD8⁺T、CD19⁺B百分率,CD4⁺T/CD8⁺T比值,IgG、IgM、IgA及尿液HCMV-DNA载量。结果 母乳阳性组和母乳阴性组、母乳高病毒载量亚组和母乳低病毒载量亚组患儿在CD3⁺T、CD3⁺CD4...     

Quantitative detection of HCMV-DNA in saliva from infants and breast milk on real-time polymerase chain reaction

Background:  The role of breast milk in viral transmission has not been fully studied. To determine the effect of breast milk on the establishment of primary human cytomegalovirus (HCMV) infection in term infants, HCMV-DNA was measured in breast milk and infant saliva.
Methods:  The study population consisted of 48 healthy term infants and their mothers. The copy number of HCMV-DNA in the infants' saliva and mothers' milk was measured on quantitative real-time polymerase chain reaction (PCR).
Results:  HCMV-DNA was detected in both saliva and breast milk from 21 infant–mother pairs, in milk only from four pairs, in saliva only from 12 pairs, and in neither from 11 pairs. HCMV-DNA was first detected in the saliva of 10 infants at age 4 months, seven infants at 7 months, 13 infants at 10 months, and three infants at 12 months. The viral loads peaked 4–10 months after birth, and thereafter decreased or became negative. The peak copy number and rate of HCMV-DNA detection in saliva were significantly related to peak copy number and rate of detection in the corresponding breast milk.
Conclusion:  Thus, HCMV passed through breast milk 1–7 months after delivery affects the persistence and level of HCMV-DNA in infant saliva and is the most important route of primary infection.     

婴儿期特发性血小板减少性紫癜与人巨细胞病毒感染的关系

目的探讨婴儿期特发性血小板减少性紫癜(ITP)与人巨细胞病毒(HCMV)感染及免疫功能的关系。方法对54例ITP患儿(病例组)及30例正常婴儿(对照组)采用酶联免疫吸附法(ELISA)行HCMV抗体检测,PCR法行HCMV DNA检测,并对病例组采用直接免疫荧光染色法行血T细胞亚群检测,比较两组差异。结果两组HCMV抗体、DNA阳性数比较,差异有显著性意义(P均<0.01)。病例组HCMV-IgM阳性与阴性者T细胞亚群CD4 、CD8 及CD4 /CD8 比较,差异有显著性意义(P均<0.01)。结论婴儿期HCMV感染可能为ITP发病的重要因素之一。免疫功能紊乱与ITP发生、发展密切相关。     

脑性瘫痪患儿150例血清TORCH抗体检测的意义

目的分析150例脑性瘫痪(脑瘫)患儿的TORCH抗体检测结果,为防治提供依据。方法对150例1个月~3岁脑瘫患儿采用间接酶联免疫吸附方法进行TORCH-IgG检测,1~6个月患儿同时检测TORCH-IgM。分析比较阳性与阴性患儿间的高危因素、脑瘫分型、并发症、头颅CT、脑干听觉诱发电位、眼底检查、脑电图等情况。结果共检测出TORCH-IgG阳性患儿58例(阳性率38.58%);阳性中以巨细胞病毒(CMV)为最常见,其次为弓形体(TOX);母子TORCH阳性符合率达85.71%。高危因素中阳性患儿以窒息、早产、黄疸、低体质量为多见,阴性患儿以窒息、早产、颅内出血为主。脑瘫分型在阳性患儿以痉挛型四肢瘫和偏瘫多见,而阴性患儿以痉挛性双瘫和偏瘫为多见。阳性患儿并发症较阴性患儿多,尤以智力低下为最明显。阳性患儿的头颅CT、脑干听觉诱发电位、眼底检查、脑电图异常率明显较阴性患儿高。结论TORCH感染是婴幼儿脑瘫的重要病因之一,对孕妇、新生儿及婴幼儿常规进行TORCH抗体检测是防治脑瘫发生的重要措施之一。     

Long-term follow-up of indirect hyperbilirubinemia in full-term Turkish infants

Objective: This retrospective follow-up study was performed to evaluate the suitability of the recently reported exchange transfusion limits (serum indirect bilirubin level of 428-496 μmol/1, 25-29 mg/dl) for Turkey. Material and methods: The study groups totalled 102 children, 8-13 years of age, who had been born at term with birthweights greater than 3000 g and had been treated for indirect hyperbilirubinemia during their newborn period; the control group consisted of 27 children of the same age-group without indirect hyperbilirubinemia. Children were grouped according to their maximum serum indirect bilirubin levels and direct Coombs'test results. Physical and neurological examinations, visual and brainstem auditory evoked potentials and the Wechsler Intelligence Scale for Children—Revised for Turkish Children were performed. Results: There was no difference between the groups with regard to mean visual and brainstem auditory evoked potential latencies. Children whose direct Coombs'tests were positive had significantly lower IQ scores and more prominent neurological abnormalities ( p < 0.05). IQ scores and prominent neurological abnormalities did not differ among the other groups. Nine children had prominent neurological abnormalities associated with abnormal brainstem auditory evoked potentials. An important risk factor was the duration that the infant's serum indirect bilirubin level remained greater than 342 μmol/1 (20 mg/dl). Conclusion: The current limit of 342 μmol/1 should continue to be used for infants whose direct Coombs'tests are positive in our country. Until better criteria for exchange transfusion other than the indirect bilirubin level are established, the current limits should also still be followed for infants whose direct Coombs'tests are negative in Turkey, where regular neonatal follow-up examinations are not satisfactory.     

肾病综合征患儿巨细胞病毒感染的初步研究

目的：探讨肾病综合征(NS)与人巨细胞病毒活动性感染的关系。方法：用聚合酶链反应(PCR)技术和间接ELISA法检测36例NS患儿外周血白细胞中的HCMV-DNA及血浆中的HCMV-IgM。结果：36例NS活动期患儿HCMV-IgM阳性11例,阳性率30.5%,HCMV-DNA阳性14例,阳性率38.9%,与对照组阳性率(12.0%,4.0%)比较差异有显著性(P<0.01);HCMV-IgM和HCMV-DNA均为阳性的有8例,阳性率为22.2%,与对照组(4%)比较差异有统计学意义(P<0.01)。结论：部分NS活动期患儿存在HCMV感染。     

婴儿癫痫与TORCH 感染的关系

目的 探讨婴儿癫痫与TORCH感染之间的关系.方法 采用ELISA法对40例婴儿癫痫患儿(癫痫组)和30例同龄健康婴儿(健康对照组)进行血清弓形虫(TOX)、风疹病毒(RV)、巨细胞病毒(CMV)和单纯疱疹病毒(HSV-Ⅰ、HSV-Ⅱ)抗体IgM、IgG水平检测.结果 癫痫组TORCH-IgM抗体阳性7例,其中CMV-IgM阳性7例,TOX-IgM、RV-IgM、HSV-Ⅰ-IgM和HSV-Ⅱ-IgM阳性例数均为0,健康对照组TORCH-IgM抗体阳性例数为0,癫痫组CMV-IgM检出阳性率高于健康对照组(P<0.05),其他TORCH系列抗体的检出阳性率与健康对照组比较差异无统计学意义(Pa>0.05).≤6个月癫痫患儿CMV-IgG抗体阳性24例(82.76%),7~12个月的癫痫患儿CMV-IgG抗体阳性5例(45.45%),≤6个月癫痫患儿CMV-IgG抗体阳性率高于7~12个月患儿(P<0.05).≤6个月癫痫患儿CMV-IgM抗体阳性6例(20.69%),7~12个月的癫痫患儿CMV-IgM抗体阳性1例(9.09%),≤6个月和7~12个月癫痫患儿CMV-IgM抗体检出阳性率比较差异无统计学意义(P>0.05).结论 婴儿癫痫与CMV感染有一定关系.     

特发性血小板减少性紫癜巨核系祖细胞巨细胞病毒感染的临床研究

目的特发性血小板减少性紫癜（ITP）是儿童常见的出血性疾病,其病因尚不十分清楚。很多研究表明,该病的发生与病毒感染密切相关。探讨人类巨细胞病毒（HCMV）感染巨核系祖细胞致ITP血小板减少的发病机制及其有效的治疗方法。方法HCMV相关ITP骨髓巨核细胞集落形成单位（CFU-MK）体外培养技术收集集落细胞,采用逆转录-聚合酶链反应（RT-PCR）检测HCMV晚期抗原基因mRNA,并给予更昔洛韦治疗。结果46例血清HCMV-DNAPCR阳性或血清HCMV-IgM阳性的ITP骨髓巨核细胞集落形成单位（CFU-MK）集落细胞HCMV晚期抗原基因mRNA阳性19例,更昔洛韦治疗有效16例;mRNA阴性27例,更昔洛韦治疗有效4例。阳性组疗效高于阴性组,P〈0．01,差异有统计学意义。结论HCMV可感染CFU—MK而成为1TP血小板减少的原因之一。巨核系祖细胞HCMV晚期抗原基因mRNA检测阳性者更昔洛韦治疗有效,能使血小板上升或恢复正常。     

Effect of gentamicin on the auditory brainstem evoked response in term infants: a preliminary report

Seven essentially healthy term infants who received gentamicin starting on the 1st day of life for prolonged rupture of membranes and maternal fever were compared with nine healthy term infants to determine whether this drug induces alterations in the auditory pathway. The auditory pathway was studied on the 3rd day of life by analyzing brainstem auditory evoked potentials elicited by a click stimulus presented at the infant's ears. Latencies of components III and V, interval I-III, and interval I-V were significantly prolonged in the gentamicin group, indicating impairment of the central component of the auditory pathway. Peak and trough serum gentamicin levels all fell within the recommended therapeutic range. The study indicates that short course gentamicin therapy in healthy newborn infants can lead to abnormality of auditory function.     

Clinical findings and adverse outcome in neonates with symptomatic congenital cytomegalovirus (SCCMV) infection

Introduction Congenital cytomegalovirus (CCMV) infection is a common neonatal infection affecting 1% of all live births, 10% of which are symptomatic. Many of these infants have long-term sequelae. The objective is to document the clinical presentation of SCCMV infection in neonates, the frequency of sequelae and severity of adverse neurologic outcomes and risk factors.Methods A review and analysis of all symptomatic infants diagnosed with SCCMV infection are given. SCCMV was defined as a diagnosis of CCMV infection in the first three weeks of life in the presence of any clinical manifestations. Outcome data from 2 years of age and later are analyzed.Results There were 104 patients identified as having SCCMV infection and of these 42 cases had definite infection. The common findings at presentation were hepatosplenomegaly 19/42 (45%), thrombocytopenia 21/42 (50%), elevated transaminases 21/42(50%), abnormal cranial US scan 24/41(56%), abnormal head CT scan 29/41(71%) and abnormal brain MRI 17/19(89%). The risk factors for an adverse outcome including death or deafness or blindness or moderate to severe neurological deficits included an abnormal cranial US scan (OR 8.5), abnormal head CT scan (OR 21) and abnormal brainstem auditory evoked responses (BAER) (OR 8.7).Conclusions There was only three (7%) patients without any deficits and severely affected infants have been identified with a diverse clinical presentation, reinforcing the importance of CMV as a major public health problem.