表阿霉素骨水泥经皮介入治疗小鼠移植瘤

确定表阿霉素骨水泥经皮介入治疗小鼠移植瘤的抗肿瘤效应。方法：３４只小鼠，体重１７－１８ｇ．Ｓ１８０肉瘤细胞１＊１０＾７个皮下成瘤后随机分两组，１ 治疗组，每个瘤块内注入含表阿霉素约１ｍｇ骨水泥０．５ｍｌ。     

高效液相色谱法测定注射用盐酸表阿霉素的含量

高效液相色谱法测定注射用盐酸表阿霉素的含量收稿日期１９９７－０２－２０蔡美明周帼雄殷璐（江苏省药品检验所,南京２１０００８）关键词盐酸表阿霉素;注射用盐酸表阿霉素;ＨＰＬＣ;含量测定表阿霉素（ｅｐｉｒｕｂｉｃｉｎ）属第三代蒽环类抗肿瘤抗生素,是阿霉素...     

多项指标联合评价表阿霉素心脏毒性的临床研究

目的：探讨表阿霉素心脏毒性的临床量化指标。方法：对60例含表阿霉素化疗的恶性肿瘤病人采用化疗前、化疗中、化疗后监测心电图心肌酶和超声心动的方法分析表阿霉素在正常心脏和异常心脏的耐量情况。结果：心脏毒性组与正常对照组的心脏毒性和累积有显著性不同。结论：表阿霉素用量与心脏状况密切相关；心脏病人的表阿霉素用量不宜超过600mg／m^2，同时应适当加用心脏保护剂。     

人肺腺癌A549细胞系多重抗药性的形成

本文采用阿霉素逐步诱导人肺腺癌Ａ５４９细胞系，在体外持续培养６个月，获得了具有抗药性的Ａ５４９／Ｒ２细胞，该细胞能在０．２μｇ／ｍｌ ＡＤＭ下持续增殖。通过测定抗癌药物作用的５０％抑制浓度发现，Ａ５４９／Ｒ２细胞对ＡＤＭ的抗药性为Ａ５４９的７－１１８倍，同时对表阿霉素、长春新碱、足叶乙甙（ＶＰ－１６）也具有显著抗药性，对氮芥、丝裂霉素和卡铂低度抗药、对５－氟脲嘧啶、氨甲喋呤和平阳霉素无抗药性。Ａ５     

国产紫杉醇联合蒽环类药物治疗晚期乳腺癌疗效观察

目的 观察紫杉醇与阿霉素、表阿霉素联合治疗晚期乳腺癌的近期疗效及毒副反映。方法 国产紫杉醇135～175mg／m^2静脉滴注3小时,d1阿霉素40～50mg/m^2静注或静滴，另一组表阿霉素60-70mg/m^2每21-28天1周期连用3周期。结果86例均可平价疗效。总有效率69.8％。初治者有效率69.65％，复治者有效率52.5％。毒副反映主要是骨髓抑制，恶心呕吐，心电图的改变。其他毒副反映均轻微可耐受。结论紫杉醇联合阿霉素，表阿霉素治疗晚期乳腺癌，可以获得较好的疗效。毒副反映可以耐受，是一个较好的化疗方案。临床可以推广应用。     

癫痫患者脑脊液中5－羟吲哚乙酸浓度变化及其临床意义

用荧光分光光度法，测定１００例癫痫患者脑脊液（ＣＳＦ）中５－羟吲哚乙酸（５－ＨＩＡＡ）的含量。结果表明，癫痫组５－ＨＩＡＡ含量（３５．８９±２．９７ｎｇ／ｍｌ）明显低于对照组（５１．１７±３．８３ｎｇ／ｍｌ），Ｐ＜０．０１。症状控制后，５－ＨＩＡＡ含量显著回升（５２．５８±２．８９ｎｇ／ｍｌ）．癫痫发作越频繁，５－ＨＩＡＡ降低越显著（２７．４０±４．７２ｎｇ／ｍｌ）。癫痫发作３天内，５－ＨＭＡ含量降低最明显（２７．０３±３．５６ｎｇ／ｍｌ）。     

表阿霉素和阿霉素诱导人肝癌细胞凋亡

目的　观察和比较表阿霉素和阿霉素诱导肝癌细胞凋亡的作用 ,为临床治疗肝癌的药物选择提供依据 .方法　用 2 0 μmol· L- 1表阿霉素和阿霉素分别作用于人肝癌细胞株HCC- 92 0 4细胞 3h,换液继续培养 8h.用细胞形态学观察、DNA电泳和流式细胞仪判定细胞凋亡情况 .结果　表阿霉素和阿霉素分别作用于 HCC- 92 0 4细胞 11h后 ,细胞形态学改变均符合凋亡细胞特征 .凋亡细胞分别占细胞总数的13.8%和 13.1% .MTT试验示各实验组与空白对照组间比较相差显著 ,表阿霉素和阿霉素相同剂量处理组之间比较无显著差异 .细胞周期检测表阿霉素组 G1 期、G2 期和 S期分别为 5 6 .4% ,10 .4%和 38.2 % ,阿霉素组分别为 6 1.1% ,8.6 %和 30 .3% ,对照组为 31.1% ,6 1.9%和 6 .0 % .结论　表阿霉素和阿霉素均能有效地诱导人肝癌细胞株 HCC- 92 0 4细胞发生凋亡 ,无明显差异 .     

悬汞电极还原计时电位溶出法连续测定生物样品中的痕量镍和钴

在盐酸－乙二胺底液中，加入丁二酮肟、盐酸羟胺、亚砂酸钠，以悬汞电极为工作电极，Ｎｉ＾２＋、Ｃｏ＾２＋产生灵敏的电位溶出峰。峰电位分别为－０．９４Ｖ、－１．００Ｖ，线性范围Ｎｉ＾２＋、Ｃｏ＾２＋能依次为０．０８－１３００ｎｇ／ｍｌ、０．０５－１１００ｎｇ／ｍｌ，检测下限分别为０．０５ｎｇ／ｍｌ、０．０３ｎｇ／ｍｌ，样品回收率均在９９％－１０５％之间。     

人粘液表皮样癌MEC─1细胞凋亡过程中细胞周期与P53的研究

人粘液表皮样癌ＭＥＣ┐１细胞凋亡过程中细胞周期与Ｐ５３的研究金军１张盈华２唐文杰１张菊２（第四军医大学唐都医院：１口腔科，２中心实验室西安７１００３８）关键词细胞凋亡阿霉素细胞周期Ｐ５３蛋白中图号Ｒ７３－３我们用１０μｇ／ｍｌ阿霉素成功地诱导了人涎腺...     

反相高效液相色谱法检测组织中阿霉素含量的实验研究

目的：建立检测心肌、肝、肾、肠组织中阿霉素含量的反相高效液相色谱法及初步应用于大鼠阿霉素肝血管输注２ｈ后心肌、肝、肾肠组织中阿霉素含量的检测。方法：取组织１．０ｇ制成匀浆，经氯仿－甲醇（４：１）混合液提取，流动相：甲醇－０．０１Ｍ醋酸钠溶液－冰醋酸（７０：３０：１）混合液（ｐＨ：８．６），流速：１．０ｍｌ／ｍｉｎ，柔红霉素为内标。采用ｗｉｓｔａｒ大鼠肠系膜静脉输注阿霉素（１５ｍｇ／ｋｇ），２ｈ后处     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.