布鲁氏菌非典型株的毒力与其过氧化氢酶活性的关系

布鲁氏菌非典型株的毒力与其过氧化氢酶活性的关系李元凯，邱海燕（中国预防医学科学院流行病学微生物学研究所北京１０２２０６）在布鲁氏菌属各个种成员过氧化氢酶活性的研究中，我们发现布鲁氏菌属六个种中，除犬种布鲁氏菌外，其他五个种过氧化氢酶活性差别不大，Ｒ型...     

布鲁氏菌非典型菌株及R型菌株生物学特性和产生原因的探讨

本文报告了布鲁氏菌非典型菌株部分生物学特性的研究，从而发现了犬种布氏菌过氧化氢酶活性高于其它种布氏菌２～８倍的特性，同时完了过氧化氢酶活性与布氏菌毒力无线性关系，并对羊种布氏菌Ｓ型、Ｒ型或Ｉ型及非典型株ＯＭＰ、ＳＤＳ－ＰＡＧＥ图谱进行了比较，找出非典型菌株不能用常规鉴定方法定种的可能原因，同时对出现非典型株原因进行了探讨。     

陕西省首次分离出犬种布鲁氏菌

近年来,一些省(区)先后报道分离出犬种布鲁氏菌。为了查明陕西省是否有犬种布鲁氏菌病的存在,于1992年4月对安康地区汉阴县清明寨乡5个村的家养犬,进行了血清学和病原学调查,结果如下。1 材料和方法1.1 抗原 犬种布鲁氏菌抗原、S型布鲁氏菌抗原、R虎红平板抗原及A、M、R诊断血清等由国中预防医学科学院流行病学微生物学研究所布病室提供。     

新疆部分地区犬种布鲁氏菌病流行病学调查报告

1986～1987年在新疆温泉、乌苏两县分离出犬种布鲁氏菌2株,在9县17个团场进行了犬种布鲁氏菌病血清学调查,用犬种抗原试管凝集反应检查狗1807只,阳性率为9.68％。     

广西犬种布鲁氏菌病流行病学调查研究

1985年在广西自治区的38个市县进行了犬种布鲁氏菌病的调查,用犬种抗原试管凝集试验对2278只家犬做了血清学检测,查出33个市县为犬种布病疫区,感染率13.7％。从19个市县分离出犬种布鲁氏菌64株,以城市郊区和平原地区的病原检出率较高,山地和丘陵地区次之。     

犬种布病与公共安全风险

布鲁氏菌包括12个已知的种型,其中牛、羊和猪种布鲁氏菌是已知的人兽共患病病原菌,引起人类较严重的感染发病。犬种布鲁氏菌主要导致犬流产和繁殖失败。虽然该菌感染人群的病例较为少见,但仍是一种潜在的威胁人类健康和动物福利的风险因素。因此,本文对犬种布鲁氏菌的病原生物学特性、致病性、流行病学、临床特征、治疗及对公众健康的影响等进行综述,为我国犬种布病的监测和防控提供参考依据。     

四川省犬种布鲁氏菌病流行病学调查

四川省犬种布鲁氏菌病流行病学调查汪立茂，付清培，薛梅，王晓，蒋和柱，李富忠（四川省卫生防疫站，成都市６１００３１）关键词犬种布鲁氏菌病；流行病学；四川省四川省位于我国西南部，以农业为主，农牧区养狗数量较多，开展犬种布鲁氏菌病的调查对促进农牧区生产的发...     

布鲁氏菌病实验室诊断研究进展

布鲁氏菌病（Brucellosis）是由布鲁氏菌（Brucella）引起的一种重要人兽共患病。根据致病性和宿主特异性,将布鲁氏菌分为6个种19个生物型,即羊种布鲁氏菌（B．meltensis）、牛种布鲁氏菌（B．abortus）、猪种布鲁氏菌（B．suis）、绵羊附睾种布鲁氏菌（B．ovis）、犬种布鲁氏菌（B．canis）和沙林鼠种布鲁氏菌（B．neotomae）。另外,     

布鲁氏菌的脂肪酸分型研究

目的探索脂肪酸分型方法对布鲁氏菌进行分型的可能性。方法选择90株布鲁氏菌经化学方法提取菌体脂肪酸后,用气相色谱分析,获得布鲁氏菌脂肪酸成分的数据资料。并利用SPSS11.5统计软件对获得的数据资料进行聚类分析。结果布鲁氏菌脂肪酸分型方法将90株布鲁氏菌分为5组:第1组为部分牛种菌;部分羊种菌;部分猪种菌;部分绵羊附睾种;部分变异牛种;部分变异羊种;沙林鼠种标准株。第2组为猪种1、2、3、5型标准株和疫苗株S2;羊种疫苗株M28和Rev.1;绵羊附睾种标准株。第3组为部分羊种;部分变异羊种菌;部分牛种3型和6型;犬种;部分绵羊附睾种。第4组为犬种菌标准株。第5组为部分羊1型;部分变异羊种;部分牛1型;部分猪1型和3型。结论依据布鲁氏菌菌体脂肪酸含量差异可以对布鲁氏菌进行分型;依据19∶0CYCLOω8c,18∶1ω7c,16∶0三种脂肪酸含量差异可以区分猪种布鲁氏菌和犬种布鲁氏菌;脂肪酸分型结果进一步提示犬种布鲁氏菌不只1个生物型;脂肪酸分型结果进一步证实牛3型和牛6型布鲁氏菌高度同源。     

桂林地区犬种布鲁氏菌病的调查

自1985年首次从本地区农村家狗中分离出犬种布鲁氏菌(B·canis)后,我们继续开展犬种布鲁氏菌病的调查研究,现将结果报告如下。 材料与方法 一、调查对象 本地区各县农村居民喂养的家狗、与狗密切接触者。 二、抗原及诊断制剂 B.canis抗原,A、M、R诊断血清及布鲁氏菌噬菌体均由中国预防医学科学     

Treatment of Brucella canis and Brucella abortus in vitro and in vivo by stable plurilamellar vesicle-encapsulated aminoglycosides

Stable plurilamellar vesicles (SPLVs) entrapping aminoglycosides were used to treat infections due to Brucella species (Brucella canis and Brucella abortus). SPLV-entrapped antibiotics effectively eliminated internalized B. canis in cultures of resident murine peritoneal macrophages and internalized B. abortus in cultures of resident guinea pig peritoneal macrophages. In vivo studies demonstrated that SPLV-entrapped aminoglycosides administered to B. canis-infected mice and B. abortus-infected guinea pigs effectively eliminated bacteria from infected organs. The dosage schedule used involved two intraperitoneal administrations of SPLV-entrapped aminoglycosides at three-day intervals. The results demonstrate the superiority of SPLV-entrapped aminoglycosides to free aminoglycosides in effecting elimination of facultative intracellular bacteria in vitro and in vivo. The use of SPLVs as a drug carrier has broad application to treatment of infections due to other organisms.     

40株地方犬种布氏菌的鉴定

我们从5个地区犬体分离40株犬种布氏菌(B.canis),在常规鉴定试验中发现,40株犬种菌有13株不被碱性复红抑制。这些菌种多数与粗糙型血清产生较高的凝集效价;所有菌种能被犬种噬菌体R/C株裂解,但对其它噬菌体不敏感,其中309号菌株为溶源菌,从中分离到噬菌体,命名Bj-309株,它能裂解所有的犬种布氏菌,但不裂解其它种布氏菌,用DNA中G C mol％法,检查了9株菌,其值为56.6～58.4,与标准犬种菌基本一致。电镜观察形态,符合于布氏菌的特征。扫描电镜见有不规则凹陷,超微切片除见有明显的脆膜和肽聚糖层外,还常见乳头状突起。     

A serological diagnostic survey for Brucella canis infection in Turkish patients with Brucellosis-like symptoms

The incidence of Brucella canis infection in humans is unknown in Turkey. In this study, we investigated the prevalence of B. canis infection in human sera obtained from six regions in Turkey and comparatively evaluated the results obtained by agglutination-based techniques using standardized antigens made from B. canis. The patients (n = 1,746) presented with clinical symptoms that were similar to those of brucellosis. All patients who tested negative in the Rose Bengal test for the smooth Brucella strains (abortus, melitensis, and suis) were screened for evidence of B. canis infection using the rapid slide agglutination test (RSAT), the microagglutination test (MAT), and the 2-mercaptoethanol RSAT test (2ME-RSAT). Of the samples tested, 157 (8.9%), 68 (3.8%), and 66 (3.7%) were positive for B. canis, as determined by RSAT, MAT, and 2ME-RSAT, respectively. The diagnostic sensitivity, specificity, positive predictive value, and negative predictive value of RSAT were 100%, 94.6%, 42%, and 100%, respectively, and of MAT were 100%, 99.9%, 97%, and 100%, respectively. We recommend the routine use of MAT and 2ME-RSAT to check the sera of all patients with symptoms of brucellosis who are negative for brucellosis using a smooth Brucella antigen.     

Br.canis感染豚鼠的实验研究

用Br.canis感染豚鼠与配种进行不同时间组合的试验表明、配种与感染 Br.canis不同时间不影响Br.canis感染后的血清学反应、皮肤过敏和细菌分布,Br.canis主要是通过性交发生感染。但在一般情况下也可以通过非性交途径发生感染。 Br.canis菌感染后机体产生凝集抗体很难与光滑型布鲁氏菌抗原发生凝集 Br.canis菌的CFT抗原作皮肤过敏试验阳性率为92.3%。可见 Br.canis菌能致敏机体。     

犬种布鲁氏菌外膜蛋白的特征及宿主牦牛对其外膜蛋白的影响

SDS—PAGE揭示出犬种布鲁氏菌外膜蛋白具有布鲁氏菌属的共同性,同时也具有其种的特征性。其独特表现为:具有一组非热修饰蛋白成分;且其微孔外膜蛋白解聚后除解聚成正常单体外,还有一组特殊的热修饰组分,此二成分受不同宿主影响而有所差异。     

高效液相色谱用于布鲁氏菌标准株的鉴别试验

用高效液相色谱法对羊种、牛种、猪种、犬种、鼠种和绵羊副睾种布鲁氏菌6个标准菌种菌株进行鉴别试验,以目视法观察菌株核酸样品的峰形数量、高度以及出峰保留时间,分析各菌株图谱的A区,结果表明:6个布鲁氏菌种都出现了各自独特的洗脱图峰,峰形清晰可辨,并且B区和C区峰形可能是布鲁氏菌属的“特征峰区”。     

Simultaneous detection of the genus Brucella by combinatorial PCR

We have developed a combinatorial polymerase chain reaction (PCR) procedure to identify four major species of the genus Brucella simultaneously. Four pairs of primers targeting the genes encoding a cell surface protein (BCSP31) and outer membrane proteins (omp2b, omp2a and omp31) were prepared. PCR using these primers gave rise to specific patterns of amplification for each Brucella spp. examined in this study. B. abortus could be identified when fragments of BCSP31 and omp2b/2a were amplified by B. abortus-specific primers. B. melitensis could be identified by the amplification of fragments of BCSP31, omp2b/2a and omp31 using pair of primers B4/B5, JRF/JPR-ab and omp31. Identification of B. canis could be achieved when the amplicons of omp2b/2a were detected by B. canis-specific primers, as could the identification of BCSP31 and omp31. If specific amplifications occurred using all pairs of primers, the strain was identified as B. suis. Combinatorial PCR reported here thus appeared to be an ideal method of identifying Brucella spp., the causative pathogen of human brucellosis.     

Investigation of the spread of Brucella canis via the U.S. interstate dog trade.

OBJECTIVES: The aim of this study was to illustrate and help address a growing need for regulatory or molecular tools to track and control the spread of canine brucellosis. Our study objectives were to first characterize Brucella canis outbreaks in Wisconsin kennels in the context of the dog trade in the USA, and then to identify a molecular technique that may be useful for strain differentiation of B. canis isolates. METHODS: Wisconsin Veterinary Diagnostic Laboratory (WVDL) B. canis serology data from 1995 to 2005 were reviewed, three canine brucellosis outbreaks in Wisconsin dog kennels were investigated, and eight B. canis isolates recovered from Wisconsin outbreaks and kennels in Missouri and Arkansas and four isolates received from outside sources were subjected to ribotyping, pulsed-field gel electrophoresis (PFGE), outer membrane protein analysis (OMPA), and cellular fatty acid profiling (CFAP). RESULTS: WVDL has received increasing numbers of B. canis positive samples from Wisconsin kennels, and Wisconsin outbreaks are associated with the interstate dog trade. All of the B. canis isolates we examined were genetically homogenous and as such could not be differentiated by ribotyping, PFGE and OMPA. However, dendrogram analysis of CFAP divided the isolates into two groups, indicating that CFAP methyl ester analysis has discriminatory power. CONCLUSIONS: CFAP methyl ester analysis has promise as a tool for epidemiological tracing of B. canis outbreaks and will be useful in comparison studies as isolation of B. canis continues to expand globally.