高效液相色谱-电喷雾质谱联用法检测大豆异黄酮和皂苷

应用高效液相色谱 电喷雾质谱联用法 ,直接对大豆胚芽的 70 %乙醇室温提取液进行检测 .根据各色谱峰的质谱特征 ,在室温提取液中鉴定出 9种异黄酮 ,7种A组皂苷 ,4种B组皂苷和2种E组皂苷 .结果证明对于复杂天然产物的定性定量分析 ,高效液相色谱 电喷雾质谱 (HPLC/ESI MS)联用分析法是一种方便快捷有效的方法 .     

大豆皂甙A的分离与ESI/MS分析

大豆胚芽中皂甙的含量是子叶的6～14倍,soyasaponin A仅存在于胚芽中.以硅胶柱色谱和制备高效液相色谱法从大豆胚芽的乙醇提取物中分离出二种A组皂甙,进行定性反应.UV和ESI/MS分析确证其结构为soyasaponin A     

大豆异黄酮软胶囊治疗黄褐斑与抗氧化能力的实验研究

目的:观察大豆异黄酮软胶囊治疗黄褐斑与其抗氧化能力间的关系。方法:采用老年雌性大鼠为实验动物模型,每天灌服大豆异黄酮软胶囊内容物,连续90天,检测服药后大鼠血及肝组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性及脂质过氧化物(MDA)含量。结论:大豆异黄酮软胶囊提高大鼠血及肝组织中SOD、GSH-PX活性(P<0.05,P<0.01),降低血及肝组织中MDA含量(P<0.05,P<0.01)。结论:大豆异黄酮软胶囊治疗黄褐斑与其抗氧化能力间有密切关系。     

高效液相色谱检测技术在肾脏疾病中的应用价值及进展

随着色谱技术的不断发展和成熟 ,高效液相色谱检测技术在疾病的诊断、治疗及疗效观察等方面发挥着越来越重要的作用。本文阐述了高效液相色谱法在肾脏疾病治疗中的应用价值及该技术的最新进展。     

高效液相色谱检测技术在肾脏疾病中的应用价值及进展

随着色谱技术的不断发展和成熟，高效液相色谱检测技术在疾病的诊断、治疗及疗效观察等方面发挥着越来越重要的作用。本文阐述了高效液相色谱法在肾脏疾病治疗中的应用价值及该技术的最新进展。     

大豆异黄酮对妇女更年期综合征及骨密度的作用研究

目的拟通过人群膳食干预试验,从临床角度研究植物雌激素大豆异黄酮对妇女更年期综合征症状及主要并发症骨质疏松的预防治疗作用。方法依据纳入和排除标准,在哈尔滨地区筛选出更年期妇女90人,随机双盲法分为大豆异黄酮剂量组和安慰剂对照组,每人每天服用大豆异黄酮90mg或者安慰剂,试验期限6个月。通过调查问卷形式记录Kupperman评分,评价更年期综合征症状改善情况;生化学方法检测试食对象血清中骨代谢指标血清钙、血清磷和血清碱性磷酸酶;超声测定桡骨远端和胫骨中段的骨密度。结果大豆异黄酮组Kupperman评分中12项指标的评分均显著下降,反应骨转换的指标血清碱性磷酸酶显著下降,胫骨骨密度显著增加,均有统计学意义。讨论大豆异黄酮可有效改善妇女更年期综合征症状,增加骨密度,预防和治疗由于更年期综合征引起的骨质疏松。     

高效液相色谱法测定注射用头孢他啶聚合物

目的 建立高效液相色谱法测定注射用头孢他啶聚合物的含量。 方法 采用高效液相色谱法,色谱柱为TSK-GEL G2000SWXL凝胶色谱柱,流动相为磷酸盐缓冲液（pH7.0）0.005 mol.L-1磷酸氢二钠溶液-0.005 mol.L-1磷酸二氢钠溶液（61：39）,流速：0.8 mL.min-1,检测波长231 nm,柱温30 ℃,进样量20 μL 。结果 头孢他啶对照品检测线性范围为0.51～25.64 μg.mL-1（r=0.999）,注射用头孢他啶聚合物检测线性范围为0.20～3.92 mg.mL-1（r=0.99）,聚合物定量限为0.71 μg。结论 该方法快速,分离度好,可用于注射用头孢他啶聚合物的检测。     

大豆异黄酮对大鼠骨密度及骨代谢生化指标的影响

目的 研究植物雌激素-大豆异黄酮对大鼠骨密度及骨代谢生化指标的影响。方法 将断乳Wister大鼠按体重随机分为4组，分别给予基础饲料和不同剂量的大豆异黄酮。每周称体重，调整给食量。12周后处死大鼠，取脏器称重，计算脏体比值；剥离股骨，测骨矿物质含量(BMC)、骨密度(BMD)和骨钙、骨磷的含量；对血清中骨形成生化指标碱性磷酸酶、骨钙素和骨吸收生化指标抗酒石酸酸性磷酸酶进行检测，同时测定雌激素-雌二醇(E2)的含量。结果 具有弱雌激素样作用的大豆异黄酮对实验大鼠的子宫、卵巢无刺激作用。与对照组相比，给予大豆异黄酮能提高BMC、BMD及骨钙含量，并随剂量的增加而增大。大豆异黄酮可影响骨代谢，高剂量的大豆异黄酮(41．6mg／kg)同时抑制骨形成和骨吸收，使骨转化率降低，但对骨吸收的作用大于骨形成。给予大豆异黄酮组血清雌激素水平大于对照组。结论 大豆异黄酮通过调整骨代谢生化指标的活性，提高大鼠的骨钙含量和骨密度，可预防骨质疏松的发生。     

护士大豆异黄酮摄入状况调查研究

目的了解护士大豆异黄酮摄入主要食物来源及频率,以指导其合理饮食,降低乳腺癌发病率。方法运用自行设计的豆类制品摄入频率调查表对104名护士进行问卷调查。结果护士大豆异黄酮摄入主要来源是软豆腐、现磨豆浆、现榨豆浆等,摄入频率幼年期均分1.25分,青春期1.43分,成年期1.41分;饮酒护士成年期摄入大豆异黄酮频率得分显著低于不饮酒护士(P<0.01)。结论护士大豆异黄酮摄入来源较广,但频率较低且摄入食物的大豆异黄酮含量相对较低。应加强关注护士大豆异黄酮的摄入,提高其对自身健康的关注度。     

HPLC分离测定红心薯中类胡萝卜素

应用高效液相色谱法分离和测定红心薯中类胡萝卜素.样品制备采用已烷-丙酮-乙腈-甲苯混合溶剂与40%氢氧化钾甲醇溶液浸提、皂化的方法,使用Nova-PakC18不锈钢柱,乙腈-三氯甲烷-叔丁醇作三元流动相,流量线性梯度洗脱等色谱条件,在440nm波长下检测,红心薯中主要类胡萝卜素得到了较好的分离.以外标定量法对红心薯中的4种主要类胡萝卜素(β-胡萝卜素,叶黄质,堇黄质,新黄质)进行了定量,结果较为满意.     

大豆胚芽异黄酮的提取分离与结构鉴定

利用柱层析法从大豆胚芽中分离到 8个异黄酮化合物 ,分别是黄豆素糖苷、大豆苷元糖苷、染料木素糖苷、丙二酰黄豆素糖苷、黄豆素苷元、大豆苷元、染料木素、染料木素葡萄糖苷 - 6″ -阿拉伯糖 ,其中化合物染料木素葡萄糖苷 - 6″ -阿拉伯糖在大豆中为新发现     

Isoflavones and their effects on the onset of puberty in male Wistar rats

This study was performed to determine how two of the most important isoflavones, genistein and daidzein, affect the gonadal axis in male prepuberal rats. One hundred and seventy‐five prepuberal male Wistar rats were allocated into seven groups: one control group and six experimental groups that were orally administered a high or low dose of genistein, daidzein or a mixture of both. Testosterone determination was assayed by EIA. The testes and body weights were measured, and the histology of the epididymis with the sperm content and epididymal sperm count were evaluated. In the control group, we observed an increase in the serum testosterone levels (>2.5 ng ml^?1) at the third week (52 days), which corresponded to the onset of puberty in these rats. The same increase in serum testosterone levels was observed at the fourth week in rats that received low doses of isoflavones; therefore, we concluded that the onset of puberty was delayed. At high doses, there was no significant increase in testosterone levels, which could be related to the fact that these male rats did not reach puberty. These findings were supported by the results obtained from the analysis of the epididymal content as well as the testes/body weight ratio.     

红车轴草异黄酮对去卵巢大鼠骨质疏松防治作用的实验研究

目的探讨红车轴草异黄酮对去卵巢大鼠引起的大鼠骨质疏松症的防治作用。方法采用切除大鼠双侧卵巢方法建立大鼠骨质疏松模型，以尼尔雌醇作阳性对照，采用放射免疫法检测实验大鼠血清雌二醇（E2）、促黄体生成素（LH）、骨钙素（BGP）、白细胞介素6（IL-6），用生物化学法检测大鼠血浆抗酒石酸盐酸磷酸酶（TRAP）。同时用定量超声检测大鼠股骨近端超声振幅衰减（BUA）及大鼠股骨骨干重和骨灰重。结果红车轴草总异黄酮可显著提高E2水平，降低LH及BGP、TRAP和IL-6活性，显著提高实验大鼠股骨干重、灰重。同时大鼠股骨近端BUA明显升高。结论红车轴草异黄酮具有提高雌激素作用，可有效抑制骨吸收，降低骨转化率，加强成骨细胞活性，增加骨密度。对去卵巢引起的大鼠骨质疏松症具有明显的防治作用。     

Isoflavones prevent bone loss following ovariectomy in young adult rats

Soy protein, a rich source of phytoestrogens, exhibit estrogen-type bioactivity. The purpose of this study was to determine if ingestion of isoflavones before ovariectomy can prevent bone loss following ovariectomy. Twenty-four nulliparous Wistar rats were randomly divided into four groups. In the normal diet groups, a sham operation was performed on Group A, while ovariectomy was performed on Group B. For Groups C and D, all rats were fed with an isoflavone-rich (25 mg/day) diet for one month, then bilateral ovariectomy were performed. In the rats in Group C, a normal diet was begun following the ovariectomy. The rats in Groups D continued to receive the isoflavone-rich diet for two additional months postoperatively. All rats were sacrificed 60 days after surgery. The weight of bone ash of the long bones and whole lumbar spine were determined. A histological study of cancellous bone was done and biochemical indices of skeletal metabolism were performed and analyzed. The markers of bone metabolism exhibited no significant changes. When compared with the sham-operated rats fed a normal diet, the bone mass of ovariectomized rats decreased significantly; pre-ovariectomy ingestion of an isoflavone-rich diet did not prevent bone loss. The bone mass of rats treated with an isoflavone-rich diet for three months was higher than controls two months after ovariectomy.     

Isoflavones and skeletal health: are these molecules ready for clinical application?

A review of the recent literature on the effects of isoflavones was undertaken to determine whether molecules such as genistein and daidzein, aglycone derivatives of soybeans, might have benefit in the prevention and treatment of osteoporosis. The current standard for science-based medicine is the documentation of efficacy of an agent under controlled, randomized, prospective conditions. A few short clinical investigations have been undertaken using isoflavones (along with soy protein), but they had limitations in study design, and the numbers of women studied were small. Other evidence from animal models, in vitro experiments, and epidemiological reports suggest that the isoflavones have skeletal benefits in women with little or no ovarian estrogen production. A clear need exists for prospective human trials, using the required conditions of randomized clinical trials and designs, to satisfy objectively the needs for science-based medicine and for appropriate clinical applications.     

大豆分级萃取

利用工业下脚料作为研究对象,通过GC/MS(气质联用)分析方法,对分级萃取物的组成、动力学进行研究,检测到下脚料中含有大量的棕榈酸、维生素E等化合物,并根据动力学确定了萃取效率.     

Soy Isoflavones May Protect Against Orchidectomy-Induced Bone Loss in Aged Male Rats

Evidence from several studies suggests that soy protein and/or its isoflavones may have beneficial effects on bone in postmenopausal women and animal models who have osteoporosis. The present study examined the dose-dependent effects of soy isoflavones in the context of soy protein or casein on the male skeleton. Thirteen-month-old male Fisher 344 rats were orchidectomized (ORX; 5 groups) or sham-operated (Sham; 1 group) and immediately placed on dietary treatments for 180 days. Diets were semi-purified and the protein source was either casein (Sham and ORX; controls), casein with two added doses of isoflavones (Iso1; 600 mg/kg diet and Iso2; 1200 mg/kg diet), soy protein with normal isoflavones content (Soy; 600 mg/kg diet), or soy protein with added isoflavones (Soy+; 1200 mg/kg diet). A 7% loss of whole body bone mineral density (BMD) was observed due to orchidectomy; however, the ORX induced BMD loss was significantly reduced to 4.3 and 4.7 % with the Soy and Soy+, respectively. Both doses of isoflavones in conjunction with casein also reduced the loss of whole body BMD, albeit not significantly different from ORX control animals. Trabecular bone histomorphometric analysis of the proximal tibia further supported the bone-sparing role of soy isoflavones as indicated by higher percent bone volume and trabecular number, and lower trabecular separation. We conclude that isoflavones exert modest beneficial effects on the male skeleton whether provided with casein or a soy protein.