速冻毛豆漂烫工艺

研究了毛豆的不同漂烫工艺,试找出最佳漂烫方式,通过对过氧化物酶失活率、蛋白质、抗坏血酸、叶绿素含量的变化及感官评定等来衡量微波处理、声热处理和热水漂烫对毛豆品质的影响.试验结果表明,声热处理1 min能有效地钝化过氧化物酶,减少蛋白质、抗坏血酸和叶绿素的损失,同时提高了毛豆的感官品质.     

过氧化物酶组化法显示皮瓣微血管的实验研究

为了探讨酶组化法显示皮瓣微血管的形态效果，本实验选Ｓ－Ｄ大白鼠鼠背皮瓣为材料，对内源性过氧化物酶组化法显示支瓣的微血管进行实验研究。结果以１０％缓冲甲醛（ｐＨ７．２￣７．４）在４℃固定６￣１２小时，冰冻切片４０μｍ联苯胺液显示微血管效果最好；鼠背皮瓣微血管网可分为五层，即浅筋薄层、肉膜层、真皮深层、乳头下层和乳头层。结论，该方法不需灌注，对血管无扩张、破裂作用，无人为改变，可用于皮瓣微血管形态学研     

蕲蛇酶溶栓和抗栓作用机理

纤溶酶原　　蕲蛇酶以全面改善纤溶系统各组份功能 ,诱导血管内皮细胞释放ｔ-ＰＡ ,ｔ-ＰＡ将纤维蛋白溶酶原变成纤溶酶 ,溶解血栓。此外 ,本品分解血纤维蛋白原 ,生成降解Ａ肽纤维蛋白单体 ,从而促进ｔ-ＰＡ激活纤维蛋白溶酶原转变成纤溶酶效应 ,促进溶栓。蕲蛇酶是目前对各种     

黄孢原毛平革菌产蛋白酶对过氧化物酶的影响

在培养黄孢原毛平革菌产生木质素过氧化物酶(LiP)和锰过氧化物酶(MnP)过程中产生的胞外蛋白酶与LiP和MnP的快速失活有关.该蛋白酶在pH 7的条件下酶活很高,其主要作用是促进LiP和MnP的分解,而不是抑制它们的产生.HgCl2是蛋白酶的抑制剂,其抑制机理类似于HgCl2对蛋白酶K(PK)的抑制.添加HgCl2的发酵液能提高过氧化物酶的酶活和稳定性,最佳添加量为1 μmol/L,最佳添加时间在接种后第5天.     

CO2超声造影在小肝癌诊断中的应用

ＣＯ２超声造影在小肝癌诊断中的应用上海医科大学中山医院超声诊断科（２０００３２）王文平近年来，超声技术的发展日新月异，如灰阶超声分辨力的提高，彩色多普勒技术的应用，使小肝癌的检出和诊断的准确性逐年提高。肝脏超声造影作为一门新的诊断技术在临床也已逐渐展...     

胃癌患者髓过氧化物酶基因多态性分析

目的 探讨髓过氧化物酶(MPO)基因多态性与胃癌发病的关系.方法 按照病例对照研究的方法,收集62例胃癌患者和61名健康对照者外周血标本,提取DNA进行MPO-463位点基因多态性检测.结果 MPO-463 GG、GA和AA 3种基因型的频率在胃癌组中分别为87.1%、11.3%和1.6%,在对照组中分别为72.1%、23.0%和4.9%;将携带MPO-463 GA和AA型者合并后与携带MPO-463 GG型相比较,患胃癌的风险显著增高(P=-0.039,DR=0.383,95%CI:0.151-0.972).相对于G等位基因,携带A等位基因的个体患胃癌的风险显著降低(P=0.026,OR:0.399,95%CI:0.174～0.916).结论 MPO-463 G/A多态性与胃癌的发生有关,其中A基因是一个保护基因.

Abstract：

Objective To investigate the association of myeloperoxidase (MPO) genetic polymorphism and gastric cancer. Methods A case-control study was performed including 62 patients with gastric cancer and 61 healthy controls. Peripheral blood was collected for genetic analysis of MPO-463. Results There were no significant differences in gender, age, and smoking between the two groups (P>0.05). However, the two groups differed in drinking, family history of gastric cancer, and Helicobacter pylori(HP) infection(P<0.05). The frequencies of MPO-463GG, GA and AA were 87.1%, 11.3% and 1.6%in the study group, and were 72.1% , 23.0% , and 4.9% in the control group, respectively. Carriers of MPO-463 GA or AA had a significantly higher risk of gastric cancer than those of MPO-463 GG (x2=4.253, P<0.05, OR=0.383, 95% CI:0.151-0.972). Carriers of G allele had a significantly lower risk of gastric cancer compared to carriers of A allele (x2=4.935,P<0.05, OR =0.399,95% CI:0.174-0.916). Conclusion MPO-463 G/A polymorphism is associated with gastric cancer with A being a protective gene.     

过氧化物酶组化法显示皮瓣微血管的实验研究

为了探讨酶组化法显示皮瓣微血管的形态效果，本实验选Ｓ－Ｄ大白鼠鼠背皮瓣为材料，对内源性过氧化物酶组化法显示皮瓣的微血管进行实验研究。结果以１０％缓冲甲醛（ｐＨ７．２～７．４）在４℃固定６～１２小时，冰冻切片４０μｍ，联苯胺液显示微血管效果最好；鼠背皮瓣微血管网可分为五层，即浅筋膜层、肉膜层、真皮深层、乳头下层和乳头层。结论，该方法不需灌注，对血管无扩张、破裂作用，无人为改变，可用于皮瓣微血管形态学研究和定量分析。     

过氧化物酶增殖物激活受体γ对大鼠肝星状细胞增殖及凋亡的影响

目的 探讨过氧化物酶增殖物激活受体γ（PPARγ）对大鼠肝星状细胞（HSC）增殖与凋亡的影响。方法 常规培养大鼠HSC，经系列浓度的PPARγ天然配体（15-d-PGJ2）或合成配体（GW7845）作用后，通过噻唑蓝（MTT）比色法及流式细胞仪观察细胞的增殖和凋亡状态，应用半定量逆转录-聚合酶链反应（RT—PCR）和Western blot探讨PPARy配体诱导凋亡的分子机制，透射电镜观察HSC形态学变化。结果15-d-PGJ2和GW7845可通过抑制细胞增殖同时诱导凋亡而抑制HSC生长，且呈剂量依赖效应（各实验组与对照组比较，P＜0．01）；PPARy活化可显著抑制HSC中bcl-2基因表达（同时在转录和转录后水平），且此抑制作用可被PPARγ特异性拮抗剂GW9662部分或完全逆转，说明此种抑制效应确由PPARγ所介导；电镜观察亦显示明显的细胞凋亡发生。结论PPARγ活化可显著抑制HSC增殖，并通过下调bcl-2基因表达而诱导凋亡，提示PPARγ可作为逆转肝纤维化治疗的新的有效靶点。     

3种猪苓营养菌丝酯酶和过氧化物酶的同工酶分析

采用聚丙烯酰胺凝胶垂直板电泳(PAGE)对3种猪苓营养菌丝中的酯酶(EST)与过氧化物酶(POD)的同工酶进行了分析.结果表明,3种猪苓营养菌丝的酯酶同工酶和过氧化物酶同工酶活性相近.酯酶同工酶共显一种谱型,5条谱带,Rf值集中在0.22～0.78间;过氧化物酶同工酶共显3种谱型,酶谱条带数在4～9条间,P.umbellatus LBZ,P.umbellatus LBJ,P.umbellatus LBT的过氧化物酶酶谱条带数依次为8条,4条,9条,Rf值集中在0.08～0.69间.聚类分析显示,P.umbellatus LBJ较其他2种猪苓亲缘关系较远,P.umbellatus LBZ和P.umbellatus LBT亲缘关系较近.     

不同治疗方法对乳腺增生病治疗效果的对照研究

目的 观察乳痛做配合推按运经仪离子导入外治对乳腺增生病乳房疼痛和肿块的临床疗效。方法 将129例乳腺增生病患者随机分为3组，以乳痛做通过推按运经仪离子导入外治龄例124乳，单用推按运经仪外治30例52乳，乳康片口服治疗30例56乳，分别对乳房疼痛和肿块进行临床疗效对照研究。结果 乳痛做离子导入外治法，对乳房疼痛痊愈率和总有效率分别为65．32％和96．77％，与其它两组对比有非常显著性差异(P＜0．01)。对乳房肿块痊愈率为17．74％，与其它两组对比有显著性差异(P＜0．01)；而总有效率为92．74％，与其它两组对比则有非常显著性差异(P＜0．01)。结论 乳痛做配合推按运经仪离子导入外治法，对乳腺增生病的乳房疼痛有较明显的改善作用，对乳房肿块也有一定的消散作用。     

Comparative Study using Four Modalities in Shinsplint Treatments*

A question of current interest and debate in physical therapy is the proper treatment of shinsplints. Various investigators have espoused the merits of different treatment programs for the clinical management of shinsplints. However, data comparing the effectiveness of the several methods is limited. This study compares the effects of ice massage, ultrasound, iontophoresis, and phonophoresis in young adults with a shinsplint syndrome. The results indicate that none of these treatment modalities was superior to another; however, all were clearly superior to a controlled treatment program. Regression analyses indicated that appropriate treatment modalities for shinsplints should be a function of the restoration of the individual's range of motion, the number of treatments that can be provided, and the availability of the treatment mode. J Orthop Sports Phys Ther 1986;8(2):77-80.     

Behavioral Treatments

Opinion statement  The central nervous system is the source of all behaviors and emotions; it also mediates the individual’s relationship with the environment. Consequently, when the central nervous system is disrupted by neurologic disease, there are frequently many concomitant emotional and behavioral disturbances, as well as conflicts with the environment and the people surrounding the patient. Patients are often unaware that these maladaptive interactions often determine the nature and quality of care that they receive from their caregivers. The aggressive or wandering brain-damaged patient often ends up in a secure facility, and the apathetic patient often becomes forgotten. Although psychopharmacologic agents can moderate some of the behavioral and emotional symptoms of brain damage, these medications have side effects such as sedation and falls, among others, and they often interfere with the metabolism of medications that patients are already taking. Behavior therapy is an excellent supplement to, if not alternative for, medications to control symptomatic behaviors associated with brain damage for the following reasons: 1) behavioral treatment is nonpharmacologic, and, therefore, there are no drug interactions or side effects in patients with neurologic illnesses; 2) behavioral treatments can be designed to treat specific symptoms, and, by mitigating them, improve the quality of life of the patient and the caregivers; 3) the success of behavioral treatments can usually be quantified as the target behaviors are pinpointed and measured before, during, and after the behavioral interventions; 4) behavioral treatments are usually cost effective, because they can be devised by psychologists, but administered by direct daily caregivers and family members; and 5) behavioral treatments administered by caregivers give the caregivers a sense of participation and control of the treatment. Reports of the effectiveness of behavioral treatments support their inclusion as an important complementary component in the care of individuals with neurologic disorders as well as the milieu of institutions that care for the brain damaged.     

不同时期重症急性胰腺炎治疗的对照研究

目的评价不同时期重症急性胰腺炎(severe acute pancreatitis,SAP)治疗的疗效。方法纳入1986年1月至2009年10月期间我院收治的234例SAP患者,按SAP收治时间的先后将其分为两个阶段:第一阶段为1986年1月至1998年8月(早期组,n=117),第二阶段为1998年9月至2009年10月(近期组,n=117),2组一般临床资料具有可比性。早期组主要施行手术和药物治疗,近期组施行多种方法联合治疗。比较2组患者的住院时间、治愈率及死亡率;住院治疗期间假性胰腺囊肿、胰腺及胰周脓肿、胰性脑病、心功能不全、急性肾功能衰竭(ARF)、急性呼吸窘迫综合征(ARDS)及休克发生率;并对2组早期治疗患者的结果以及腹腔积液、胆源性胰腺炎和胰腺及胰周并发症的治疗结果进行分层对比。结果与早期组比较,近期组的住院时间短(P<0.05)、治愈率高(P<0.001)及死亡率低(P<0.001)。近期组治疗期间假性胰腺囊肿、胰腺及胰周脓肿、胰性脑病、心功能不全、ARF、ARDS及休克发生率均低于早期组(P<0.05)。根据分层对比结果,近期组采用早期治疗以及腹腔积液、胆源性胰腺炎和胰腺及胰周并发症的治愈率...     

不同方法治疗后咬肌厚度减少的比较研究

目的通过比较三种方法治疗后的咬肌厚度变化，评价不同方法在减少咬肌厚度中的应用价值。方法自2005年8月至2006年12月，对13例下面部缩窄整形患者，于术前应用B超检查，术后12周、24周测量咬肌厚度。结果下颌角截骨、下颌角截骨＋咬肌部分切除及下颌角截骨＋咬肌注射A型肉毒素三种治疗方法，术后24周，咬肌厚度平均减少2．637％、30．286％和36．878％。结论下颌角截骨＋咬肌注射A型肉毒素比下颌角截骨＋咬肌部分切除或单纯下颌角截骨手术能更有效地减少咬肌厚度。     

EDTA对木质素过氧化物酶在染料脱色中的影响

在反应液中含 2mmol/LEDTA时 ,2 4h后染料甲基橙的脱色率比未加EDTA的下降约70 % .添加 2mmol/LEDTA明显地抑制了木质素过氧化物酶 (LiP)对多种染料的脱色 .酶动力学研究表明 :EDTA对LiP的抑制属非竞争性抑制 .添加比EDTA稍大量的Zn2 + 能解除EDTA对LiP的抑制 ,其他多种金属离子也能减轻EDTA的抑制作用 .