共查询到14条相似文献,搜索用时 234 毫秒
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目的:观察白介素6(IL-6)和神经生长因子(NGF)在成年雌性大鼠、卵巢切除大鼠、卵巢切除后雌二醇替代大鼠心内神经节的表达及变化.方法:免疫组织化学方法.结果:各组大鼠心内神经节均存在IL-6阳性神经元和NGF阳性神经元,但卵巢切除组大鼠心内神经节IL-6阳性神经元明显增多,表达明显增强,而卵巢切除组大鼠心内神经节NGF阳性神经元的数量却明显减少,表达明显降低.结论:心房后壁心内神经节存在IL-6和NGF,并且受雌激素影响,提示雌二醇可能影响心内神经节IL-6和NGF的表达. 相似文献
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目的 观察白介素-6(IL-6)在成年雌性大鼠、卵巢切除大鼠、卵巢切除后雌二醇替代大鼠心内神经节的表达及变化.方法 免疫组织化学方法.结果 各组大鼠心内神经节均存在IL-6阳性神经元,但卵巢切除组大鼠心内神经节IL-6阳性神经元明显增多,表达明显增强.结论 心房后壁心内神经节存在IL-6,并且受雌激素影响,提示雌二醇可能影响心内神经节IL-6的表达. 相似文献
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目的:观察神经生长因子(NGF)在卵巢切除大鼠心房后壁心内神经节的表达及雌激素的调节作用.方法:实验动物分正常组、卵巢切除组、卵巢切除后雌二醇替代组,各组动物审温下饲养3周后,进行 NGF 免疫组织化学显色.结果:各组大鼠心内神经节均存在 NGF 阳性神经元,该类神经细胞胞体多数呈圆形或椭圆形,大、中、小型细胞均有发现.正常组和卵巢切除后雌二醇替代组 NGF 免疫阳性神经无约占全部神经节细胞总数的比例分别为 61%和64%,但卵巢切除组大鼠心内神经节 NGF 阳性神经元的数量明显减少,表达明显降低,NGF 免疫阳性细胞约占全部神经节细胞总数的比例为28%.结论:心内神经节细胞内含 NGF;雌激素可能影响心内神经节细胞的 NGF 表达. 相似文献
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用免疫组织化学和原位杂交的方法观察成年(3月龄)大鼠和中老年(15月龄)大鼠心内神经节雄激素受体(androgenreceptor,AR)蛋白及其mRNA的表达和变化。结果显示:成年及中老年大鼠心内神经节均存在AR阳性神经元和AR mRNA阳性神经元,但中老年大鼠心内神经节AR阳性神经元和AR mRNA阳性神经元数目明显减少,表达明显降低。这些结果为AR在心脏的存在提供了形态学依据,并且证实心内神经节部分神经元能够合成AR,提示心内神经节可能受雄激素影响。 相似文献
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应用细胞原位杂交技术,观察经重组小鼠白细胞介素-19(IL-1β)处理后的体外培养的新生1d大鼠中脑黑质神经元c-jun基因的表达.结果显示,培养的黑质细胞多为酪氨酸羟化酶阳性神经元,IL-1β可诱导体外培养的黑质神经元c-junmRNA表达,高水平的表达出现在IL-1β处理后2~4h。说明IL-1β有兴奋黑质神经元的作用,并提示黑质神经元上可能存在IL-1β受体. 相似文献
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Sáinz J Osuna A Wangensteen R de Dios Luna J Rodríguez-Gómez I Duarte J Moreno JM Vargas F 《Experimental physiology》2004,89(2):155-162
In this study we have evaluated the influence of sex, gonadectomy and sex hormones on the development of L-NAME-induced hypertension in the rat, focusing our investigation on blood pressure (BP), plasma renin activity (PRA), cardiac hypertrophy and proteinuria. Three experiments were performed to investigate: (i) the influence of sex on the development of L-NAME-induced hypertension; (ii) the effects of gonadectomy on the dimorphism of L-NAME-induced hypertension; and (iii) the effects of testosterone in ovariectomized female and of 17beta-oestradiol in orchidectomized male rats. Male L-NAME-treated rats had higher BP values than females. Orchidectomy of L-NAME-treated rats reduced BP to the levels of females and ovariectomy did not affect hypertension in females. Oestrogenized and orchidectomized males had a BP level similar to intact female L-NAME-treated rats. However, androgenization and ovariectomy did not change BP in female L-NAME-treated rats. PRA was greater in intact male L-NAME hypertensive rats than in female rats, and gonadectomy protected against the increase in PRA such that PRA was similar among all the groups. Intact female hypertensive rats showed significantly greater ventricular hypertrophy compared with male hypertensive rats. Male L-NAME hypertensive rats had increased proteinuria that was not present in female rats. Moreover, testosterone increased proteinuria in males and females regardless of the BP level. Male L-NAME-treated rats developed higher BP, PRA and proteinuria than female rats, but were more resistant to the development of cardiac hypertrophy. The higher PRA of male L-NAME-treated rats might be involved in the sex-dependent dimorphism of this model of hypertension. 相似文献
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用免疫细胞化学双重标记技术研究雌激素受体(estrogen receptor,ER)和神经生长因子(NGF)的表达,证实它们在心内神经节共存的可能性。结果显示,在心内神经节可以观察到3种细胞:(1)ER单标细胞,胞核呈棕褐色;(2)NGF单标细胞,胞浆呈红色;(3)ER/NGF双标细胞,胞核棕褐色,胞浆为红色。双标细胞占全部阳性标记细胞的30%~40%。这些结果提示ER和NGF可共存于同一大鼠心内神经节细胞,提示雌激素和NGF在维持心内神经节的结构和功能方面存在复杂的交互作用。 相似文献