Experiments on Gene Transferring to Primary Hematopoietic Cells by Liposome

GenetransferintohematopoieticcelIshasmanyexperimentalaswellasclinicalapplications['].Atpresenttime,thestrategyofgenetransferusedmostlyistheretroviralvectorsystem['].However,theuseofretroviralvectorssuffersfromseveraldrawbacks:(l)thegenerationandmanufacturingofretroviralvectorsarecomplexandnotinexpensive;(2)generalsafetyconcernsareassociatedwiththeuseofanyviral--basevectorssystem,includingrepli-cationdefectivemurineretroviraIvectors;(3)retro-viralvectorscurrent1yusedareabletotransduceon1ydividi…     

Gene Transfer into Hematopoietic Cells of Mouse and Its in vivo Expression after Transplantation

Genetransferintohematopoieticcellshasfoundmanyapplicationinexperimentalresearchesaswellasclinicalpractice['1.Atpresent,thestrategyofgenetransferusedmostcommonlyistheretrovirusvectorsystem[2].However,theuseofretrovirusvectorssuffersfromseveraldrawbacks:(1)thegenerationandmanufacturingofretrovirusvectorsarecomplexandnotinexpensive;(2)generalsafetyconcernsareassociatedwiththeuseofanyviral--basevectorsystem,includingreplicationdefectivemurineretrovirusvectorsj(3)retrovirusvectorscurrentlyusedareab…     

Construction and Expression of Human PTEN Tumor Suppressor Gene Recombinant Adenovirus Vector

Phosphatase and tensin homologue deleted onchromosome10(PTEN)is a newtumor suppres-sor gene and possesses mutation in multiple kindsof tumors which include breast cancer[1].Teng[2]found the incidence for loss of heterozygosity(LOH)in breast cancer speci mens was48%(32/67),and the mutation and inactivation of PTENgene plays a central role in cell migration,growthandinvasion of breast cancer[3].This study ai ms atestablishing a kind of proliferative defective recom-binant adenovirus vector A…     

Abnormal Change of p53 Gene in Gastric and PrecancerousLesions and APC Gene Deletion in Gastric Carcinoma and Near Tissues

Hypothesisoftumorsuppressorgenehadbeenproposedtwentyyearsag0['],butthefirstsuppressorgeneRb(retinoblas-toma)wasseparatedandcloneduntill986[z].Suppressorgene,includingp53andAPCgenes,n0whasbecomenew"hot"subjectintheareaoftumorresearch.p53genemutation,deleti0nandAPCgenedele-tionoccurredinsomehumantumorsandtheremaybesomerelationbetweenAPCgeneandp53genedeletion[3-7]-Inthispa-per,PCR-RFLP,PCR-SSCPmeth0dswereusedtoanalyzep53,APCgenealterationsingastriccancerandprecancerouslesi0nsinordertoin…     

Expression of the Tumor Metastatic Suppressor Gene in Mouse Melanoma Model: Inverse Association to Metastatic Potential

Cancermetastasisisacomplexprocessinwhichtumorcellsofaprimaryneoplasminvadenormaltissues,enterandescapethevascularture,andgeneratesecondarytumorsormetastasessequentialsteps,eachofwhichislikelytoentailtheactivationand(or)repressionofspecificgenesorgeneproducts,dependingontileoriginandtypeoftumor[1'2].Atleast10differentoncogenshavebeenshowntoaugmentthetnetastasesofavarietyoftumorsinexperimentalam--.inals.The"m23isonesuchgenereportedtobedown--regulatedinmetastaticcellsofdifferentrodenttumorsL3].Th…     

Effect of Antisense MBD1 Gene Eukaryotic Expression Plasmid on Expression of MBD1 Gene in Human Biliary Tract Carcinoma Cells

DNAmethylationhasavarietyofimportantfunctionsintheregulationofgeneexpression[1].ManystudiesdemonstratedthatthepathogenesisofbiliarytractcarcinomawascloselyassociatedwiththeaberrantDNAmethylationstatusoftumorrelatedgenes.TheaberrantstatusofDNAmethylationincludedthehypomethylationofonco genesandthehypermethylationoftumorsuppres sorgenes(TSG).IncreasingtheexpressionofTSGandimprovingthefunctionofTSGbyelimi natingitshypermethylationstatusmaybeanewwaytotreatbiliarytractcarcinoma.Toexplorether…     

Relationship between Invasiveness of Pituitary Somatotrophinomas and Structural Abnormalities of Protein Kinase C Gene in Human

Morerecently,attenti0nhasbeenf0-cused0nthepotentialrolesofmembranephosphatidylinositol(PI)hydrolysisandproteinkinaseC(PKC)ins0mat0trophfunction[2-4'9].PKCactivityandexpressionwerefoundtobesignificantlyhigherinhu-manpituitarytumorscomparedwithnormaltis.ue.[3.9]-AlteredPKCstructurewasdemonstratedt0beassociatedwithinvasive-nessoftumors[4],suggestingthatthealphaisoform0fPKC(aPKC)mayharboras0-maticmutati0nincodon294,therebyre-sultinginsubstitutionofasparticacid(GAC)byglycine(GGC)inacorresp…     

Magnetic Iron Oxide Nanoparticles Mediated Gene Therapy for Breast Cancer-An In Vitro Study

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), also designated as Apo-2 ligand, is a typical member of the structurally related tumor necrosis factor (TNF) ligand family. It has been shown that TRAIL only induces the apoptosis of cancer cells rather than normal cells. This characteristic offers a new target for cancer treatment[1]. In fact, TRAIL gene has been successfully used for the treatment of cancer[2]. Viruses have been the common vectors used in TRAIL gene t…     

Cloning of Human Uroplakin Ⅱ Gene from Chinese Transitional Cell Carcinoma of Bladder and Construction of Its Eukaryotic Expression Vector

In 1998, Wu et al successfully cloned humanUroplakin Ⅱ (UPⅡ) gene from the samples of oc cidental patients with bladder transitional cell car cinoma ( TCC) [1]. Since then, a lot of researchfindings indicated that UPⅡ gene was 100 % posi tively expressed in tissues of TCC, and it is be lieved that the specific expressed gene of bladderTCC has obvious significance in the diagnosis andtargeted therapy of TCC[2]. Up to now, UPⅡgene was only completely …     

Induction of Osteocalcin Gene Expression In Vitroby Progesterone

Sinceestrogendeficiencyisidentifiedasthemainreasonforthepostmenopausals-teoporosis,therepracementtherapybyes-trogenhasbeenwidelyacceptedasamajorapproachforthepreventionandtreatmentofthisdisorder.However,itwasnoticedrecentlythattheproductionofprgesteronebypostmenopausa1wmenwasalsre-duced[l].Clinica1studieshaveshowedthattreatmentwithprgestinsalonecanpreventbonelosseffectivelyaftermenopauseL'],mostlikelybyincreasingbnefrmation,assuggestedbyelevatedlevelsfserumal-kalinephsphataseandserumGLAprtein…     

Role of the Pentanucleotide (tttta)_n Polymorphisms of CYP11αGene in the Pathogenesis of Hyperandrogenism in Chinese Women with PolycysticOvary Syndrome

Polycystic ovary syndrome (PCOS) is a heteroge neous endocrine disease in women, which is charac terized by hyperandrogenism, chronic anovulation,infertility, high serum level of leuteinizing hor mone and high ratio of LH/FSH[1, 2]. Althoughthe precise models of inheritance are still unclear,strong familial aggregation suggests a major genet ic component to the etiology of PCOS[3, 4]. Recentyears, increasing studies have demonstrated thatmicrosatellite ( tttta)n poly…     

Effect of Wild-type p53 Gene Transfection on the Growth and Radiotherapeutic Sensitivity of Human Glioma Cells

Ingliomas,p53mutationsareamongthemostfrequentlyobservedgeneticfindings[1].Asatumorsuppressorgene,p53mutationcanleadtouncon trolledcellproliferation[2].Itwasreportedthattransfectionofwild typep53genemayenhancetheradiosensitivityoftumorcellscontainingwild typep53[3].Inthisstudy,wild typep53genewasde liveredintohumangliomacelllineU251byusingliposome mediatedtransfectiontechniquetoob servetheeffectofp53genetreatmentandcom binedtreatmentofp53andirradiationtherapyongliomacell.1MATERIALSANDMET…     

Relationship between Single Nucleotide Polymorphism in TNF-α Gene Promoter Region and Inhibitory Effects of Triptolide on TNF-α Production in Peripheral Blood Mononuclear Cells of Healthy Humans

Tumor necrosis factorα(TNF-α)is ani mpor-tant cytokine that has extensive biological activity.It plays key rolesinthe pathogenesis of rheumatoidarthritis(RA).The TNF-αlocus has yielded a va-riety of polymorphic sites such as-308,-238, 498andso on[1].Amongthese polymorphic sites,ithas been demonstrated that TNF-α-308single nucle-otide polymorphism(SNP)is closely related withthe susceptibility,response to drugs and outcomeof RA[2-5].Triptolide that is a main active compo-nent of Tript…     

Development of neural precursor cells from mouse embryonic stem cells

Embryonicstemcells(EScells)arederivedfromtotipotentcellsofearlyembryoandpossessunlimited,undifferentiatedproliferationinvitro[1].EScellshavetheinherentcapacityofdifferentiatingintoallcelltypesofthenervoussystemasdemonstratedbytheex-perimentthatchimericmicewereformedfromem-bryosinwhichEScellsaretransplantedintotheinnercellmass[2].ThesuccessfulestablishmentofhumanEScelllineindicatedprofoundimplicationthatEScellsmayprovideavirtuallyunlimiteddonorsourcefortransplantation[3]. Threepaperspublish…     

Transfection of Antisense Oligodeoxynucleotide Inhibits Heparanase Gene Expression and Invasive Ability of Human Pancreatic Cancer Cell in vitro

Cancer cell invasion and metastasis are criticalsteps in pancreatic cancer progression that worsenthe prognosis.They are facilitated via disassemblyof the main structural elements of basement mem-branes(BM)and extracellular matrix(ECM)byenzymes produced by the cancer cells[1].Hepara-nase(HPSE)is an endoglycosidase responsible forthe degradation of the heparan sulfate proteoglycan(HSPG),a major component of ECM and BM.HPSE mediates tumor invasion pri marily throughthe direct cleavage of…     

Eukaryotic Expression of Human Arresten Gene and Its Effect on the Proliferation of Vascular Smooth Muscle Cells

Arresten,human noncollagenous domain 1 oftheα1 chain of type Ⅳcollagen,is derived fromthe carboxy terminal of type Ⅳcollagen[1].It wasidentified as a powerful inhibitor of angiogenesisrecently .In earlier studies , human arresten wassuccessfully amplified from placenta tissue , andmolecular cloning and DNA sequencing verifiedthat the coding sequence obtained was correct[2].Prokaryotic expression vector of arresten gene hasbeen constructed[3]. Excessive proliferation andmigration to endome…     

Treatment of Hepatitis Caused by Cytomegalovirus with Allitridin Injection──An Experimental Study

Hepatitiscausedbyhumancy-tQmegalovirus(HCMV)isquitecommona-monginfantsinChina.ltusuallylastsforseveralmonthsandevencausesthedeathinseverecongenitallyinfectedpatients['].Al-thoughganciclovirhasbeenusedasana-gentofchoicetotreatadults'CMVhepati-tisthatdevelopedafterorgantransplanta-tion[2],side-effectssuchasmyelotoxicitylimititsuseininfants[3].ItwasreportedthatgarlicextractcouldinhibitthegrowthofCMVADl69(strainforroutinelaborato-rywork)invitro[4J,whichmeantthattheremaybeanalternativesubstan…     

Association of Angiotensin Converting Enzyme Gene I/D Polymorphism With Type 2 Diabetes Mellitus

INTRODUCTION Type 2 diabetes mellitus (T2DM) is a common multifactorial disease with genetic predisposition that is strongly influenced by environmental and behavioral factors, such as obesity and sedentary lifestyle. Previous studies have indicated that obesity, central obesity, physical inactivity, and family history of the disease are major risk factors for T2DM[1]. Furthermore, the high angiotensin II level and low bradykinin level in the renin-angiotensin system (RAS) related to …     

Studies on the Relationship between Urokinase Plasminogen Activator(uPA)and Human Sperm Motility

Plasminogenactivators(PAs)arehighlyselectivetrypsin--likeserineproteaseabletoconverttheinactiveplasminogen,azymogenpresentinmostextracellularfluids,intoplasmin,alsoatrypsin--likeproteaseofbroadspecificity['].SeminalplasmalevelsofPAsinmenshowup60to100foldhigherthaninbloodplasma['].ThetwoknownPAs,urokinase--type(uPA)andtissue-type(iPA)aresecretedbyalargenumberofcellsandarethoughttoexhibitmultiplefunctionsinextracellularproteolysisaccompanyingtissueremodelingandcellmigration[']'Duetotheseprop…