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目的 分析北京市海淀区食源性腹泻患者致泻大肠埃希氏菌(diarrheagenic Escherichia coli,DEC)的感染状况、型别分布和耐药情况,为致泻大肠埃希氏菌的防控和临床合理用药提供科学依据。方法 对2015~2019年海淀区食源性疾病监测哨点医院送检的1 810份腹泻患者粪便标本直接划线接种在麦康凯培养基上培养,挑取可疑菌落进行生化鉴定、毒力基因测定,对确认为致泻大肠埃希氏菌的菌株进行药敏检测。结果 1 810份粪便标本中检出致泻大肠埃希氏菌214株,检出率为11.8%,各年度的检出率差异有统计学意义(χ2=10.858,P=0.028)。检出的致泻大肠埃希氏菌共有4种型别,其中集聚性大肠埃希氏菌(EAEC)96株(44.9%),产肠毒素大肠埃希氏菌(ETEC)89株(41.6%),肠道致病性大肠埃希氏菌(EPEC)24株(11.2%),肠道侵袭性大肠埃希氏菌(EIEC)5株(2.3%)。在12种抗生素的药敏试验中,70株(32.7%)全部敏感,144株(67.3%)耐药;氨苄西林的耐药率最高(54.7%),其次是四环素(38.8%)和复方磺胺(36.9%),对3种及以上抗生素的多重耐药率为46.3%。结论 北京市海淀区2015~2019年致泻大肠埃希氏菌感染以EAEC和ETEC为主,耐药菌株的多重耐药谱广。应加强对抗生素的使用管理,延缓耐药株的产生和传播。 相似文献
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目的 明确本院感染性腹泻患者致泻性大肠埃希菌毒力和耐药特征。方法 采用VITEK MS微生物质谱检测系统初步鉴定,多重实时荧光PCR检测毒力基因,对本院感染性腹泻患者临床分离的致泻性大肠埃希菌(Diarrheagenic Escherichia coli, DEC)进行5种型别鉴定。微量肉汤稀释法和E-test法药敏试验检测DEC菌株的耐药表型特征。二代测序及生物信息学分析其耐药分子特征。采用Fisher确切概率法进行统计学分析。结果 本院DEC检出率为11.9%,其中EAEC占比37.5%,非典型EPEC占比34.38%,ETEC占比25.0%,EIEC占比3.12%,未检出EHEC菌株。32株DEC对氨苄西林、四环素、甲氨苄啶/磺胺异恶唑耐药率最高,分别为53.12%、43.75%和37.5%。ESBLs(+)株占比18.75%,其多重耐药菌株检出率为83.83%,显著高于ESBLs(-)菌株,差异有统计学意义(P=0.042)。32株DEC共有25个ST型,优势基因型为ST10共4株(12.5%),ST28、ST31和ST3153各2株(各占比6.25%),其他21个型别各1株菌... 相似文献
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引起腹泻的大肠埃希菌可分为6种,包括肠聚集大肠埃希菌(enteroadherent estherichia coli,EAEC)、肠出血性大肠埃希菌(EHEC)、肠致病性大肠埃希菌(enterophathogenic escherichia coli,EPEC)、肠产毒性大肠埃希菌(enterotoxin of escherichia coli,ETEC)、肠侵袭性大肠埃希菌(enteroinvasive escherichia coli,EIEC)和弥散性粘附大肠埃希菌。EAEC作为儿童腹泻源的重要性仍有争论, 相似文献
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《中国实验诊断学》2020,(10)
目的了解吉林省食源性疾病患者中致泻大肠埃希氏菌的毒力基因分布、流行特征、耐药情况、以及PFGE分子分型特征,为致泻大肠埃希氏菌的监测、爆发预警、耐药抗生素规避使用提供依据。方法对2016年从吉林省食源性疾病患者粪便中获得的26株致泻大肠埃希氏菌进行生化鉴定、多重PCR检测毒力基因、MIC微量肉汤稀释法药敏实验以及PFGE脉冲场凝胶电泳分子分型。结果26株致泻大肠埃希氏菌中13株(50%)为EAEC;7株(26.92%)为EPEC;4株(15.38%)为ETEC;2株(7.69%)为EHEC;对四环素、甲氧苄啶/磺胺甲噁唑、环丙沙星耐药极为严重,分别高达92.30%、88.46%和84.62%;XbaI酶切后进行PFGE脉冲场凝胶电泳分为23个型别。结论吉林省腹泻患者中致泻大肠埃希氏菌感染类型以EAEC为主,存在混合感染情况;耐药、多重耐药情况较为严重应加强规范抗生素的使用;在DNA水平上呈现出多态性。 相似文献
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引起腹泻的大肠埃希菌可分为6种,包括肠聚集大肠埃希菌(enteroadherent escherichia coli,EAEC)、肠出血性大肠埃希菌(EHEC)、肠致病性大肠埃希菌( enterophathogenic esche richia coli,EPEC)、肠产毒性大肠埃希菌(enterotoxin of esche richia coli,ETEC)、肠侵袭性大肠埃希菌(enteroinvasive esche richia coli,EIEC)和弥散性粘附大肠埃希菌。EAEC作为儿童腹泻源的重要性仍有争论,本文采用 PCR方法检测 EAEC的质粒 pCVD432,从而判定EAEC在瑞士腹泻儿童中的重要性。1.资料与方法:用标准的培养方法和 PCR方法测定 Zu… 相似文献
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目的了解儿童肠道感染中致泻性大肠埃希菌的感染情况。方法对95份腹泻患儿粪标本中分离出的大肠埃希菌,用荧光聚合酶链反应(PCR)的方法检测毒力基因,鉴定致泻性大肠埃希菌。结果检测出15株阳性菌株,阳性率为15.8%,其中肠聚集性大肠埃希菌(EAEC)6株、肠致病性大肠埃希菌(EPEC)4株、弥散聚集性大肠埃希菌(DAEC)4株和肠产毒性大肠埃希菌(ETEC)1株。结论致泻性大肠埃希菌是引起儿童腹泻的主要致病菌,临床实验室应加强对致泻性大肠埃希菌的检测。 相似文献
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目的了解长沙市食品从业人员致泻大肠埃希菌感染、分布及毒力基因携带情况。方法抽取长沙市9个单位258例食品从业人员为研究对象,采用实时荧光多重聚合酶链反应(PCR)检测致泻大肠埃希菌。结果致泻大肠埃希菌感染率为15.5%(40/258),其中肠聚集性大肠埃希菌(EAEC)占10.9%(28/258),肠致病性大肠埃希菌(EPEC)占3.1%(8/258),肠出血性大肠埃希菌(EHEC)占1.6%(4/258),未检出肠侵袭性大肠埃希菌(EIEC)和肠产毒性大肠埃希菌(ETEC)。28株EAEC携带uidA基因26株,aggR+pic基因13株,astA基因27株,携带率分别为92.9%、46.4%、96.4%;8株EPEC全部携带eae和uidA基因;4株EHEC中3株携带eae基因,且都携带stx1+stx2基因。结论建立持续监测致泻大肠埃希菌的机制和深入研究其分子流行病学理论,除了为监管部门制订和评价公共卫生措施提供基础数据,对评价食品安全状况,有效控制传染源、保护人民群众健康具有重要意义。 相似文献
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目的 建立一种能够快速检测并分型5种致泻性大肠埃希菌、志贺菌及沙门菌的多重聚合酶链反应(multiplex polymerase chain reaction,M-PCR) 方法。 方法 设计针对7种常见肠道致病菌的12对引物,通过多重PCR方法扩增后电泳观察相应条带,确定病原菌。临床标本直接划线接种于肠道选择性平皿,挑取可疑菌落直接提取核酸进行多重PCR检测。 结果 所有标准菌株均能扩增到相应目的片段,322份腹泻病标本中使用该方法共检出志贺菌24株(福氏志贺菌7株、宋内志贺菌17株)、 沙门菌5株、EPEC共12株(均为aEPEC)、ETEC共6株(elt阳性3株;est阳性3株)、EIEC共3株、VTEC共1株(stx1和stx2A均阳性)、EAEC共3株。 结论 该方法快速特异,能同时检测7种常见肠道致病菌,可用于腹泻病常见病原的快速检验。 相似文献
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Background
Diarrheagenic Escherichia coli (DEC) signifies as an important etiological agent of moderate‐to‐severe diarrhea. This study was primarily focused on molecular identification of DEC pathotypes; their association with serogroups and estimates of resistance profiles against different antibiotics regime.Methods
Five hundred seventy‐two stool specimens from diarrhea patients were investigated for DEC pathotypes. Molecular pathotypes were identified by amplification of virulence genes associated with distinct pathotypes followed by sequencing. Diarrhea is a self‐limiting disease, however, severity and persistence of infection suggest antibiotic use. Therefore, AST and MIC were determined against common antibiotic regimen. Correlations between molecular pathotypes and serogroups were analyzed by somatic “O” antigen serotyping.Results
The present findings reveal incidence of DEC as an etiological agent up to a level of 21% among all diarrheal age groups. DEC infection rate was higher in children. Enteropathogenic E. coliEPEC, a molecular pathotype of DEC, was found as a predominant pathotype with highest frequency of 13.7%. Two other molecular pathotypes enterotoxigenic E. coli (ETEC) and enteroaggregative E. coli (EAEC) accounted for 5.7% and 1.3%, respectively for all diarrhea incidences. Serological analysis deciphered somatic antigens O26, O2, and O3 as major serogroups identified among EPEC, ETEC, and EAEC pathotypes, respectively. All DEC pathotypes exhibited high levels of antibiotic resistance except for cotrimoxazole and norfloxacin.Conclusion
Comprehensive molecular characterization of DEC pathotypes, their incidence estimates, and antibiogram patterns will help in ascertaining better diagnostic and therapeutic measures in management of diarrheal diseases.15.
Diarrheagenic Escherichia coli (DEC) is a set of the most common pathogens causing diarrhea. DEC strains are classified into five pathotypes based on the possession of different virulence genes: enteropathogenic E. coli (EPEC), enterohemorrhagic E. coli (EHEC) or Shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), and enteroinvasive E. coli (EIEC). The development of an easy-to-use method to detect the specific virulence genes and distinguish the pathotypes is essential for the diagnosis and surveillance of DEC infections. In this study, a multiplex PCR assay (mPCR) specific to nine virulence genes and an internal control was designed for the identification of five DEC pathotypes. A temperature switch PCR (TSP) strategy was used in the PCR amplification. The PCR products were detected by capillary electrophoresis. The limit of detection (LOD) of the 10-plex reaction was 5 × 103 copies/reaction for stx2 and 5 × 102 copies/reaction for the other targets. The mPCR showed very high specificity, and inclusivity and exclusivity were both 100%. When the mPCR assay was used for the detection of 221 cryopreserved diarrhea specimens, DEC colonies were detected from 49 specimens, and the positive rate was 22.2%. The mPCR assay was sensitive and specific, and the amplified product could be analyzed easily. Thus, this method could be used effectively to identify the suspected colonies of DEC in the primary culture of the specimen. 相似文献