皮神经营养血管皮瓣结合负压封闭引流在创面修复中的应用

目的 探讨皮神经营养血管皮瓣结合负压封闭引流在复杂创面修复中的临床效果.方法 入选患者26例,随机分为试验组和对照组:试验组13例,创面先经负压封闭引流处理5～7 d,碘伏换药2～5 d,再行皮神经营养血管皮瓣修复;对照组13例,创面常规换药处理后行皮神经营养血管皮瓣修复.观察两组患者创面准备时间、皮瓣成活及创面愈合后外形与功能恢复情况.结果 24例患者皮瓣一期存活,试验组和对照组各有1例皮瓣远端部分坏死,经换药后愈合.治疗过程中无大出血、血管神经损伤、感染及皮瓣撕脱等并发症.所有患者均获随访,随访时间12～18个月,皮瓣质地优良,外观无明显臃肿,功能满意.试验组创面准备时间为(9.8±3.2)d,对照组创面准备时间为(14.2±4.5)d,试验组创面准备时间比对照组要短,差异有统计学意义(t=3.522,P＜0.05).结论 皮神经营养血管皮瓣结合负压封闭引流能够缩短治疗时间,成功率高,临床效果满意,是修复各种复杂创面的理想方法之一.     

负压封闭引流治疗难治性创面的护理

目的:总结负压封闭引流治疗难治性创面的护理体会。方法:对46例难治性创面采用负压封闭引流治疗的患者进行观察及术前术后各项护理。结果:难治性创面采用负压封闭引流治疗,创面的渗出物和坏死组织被及时清除,肉芽组织新鲜,为植皮或皮瓣转移修复创造了良好条件。结论:对临床上难治性创面采用负压封闭引流技术治疗,效果良好。护理要点:加强心理疏导,提高患者对创面愈合的信心,做好负压封闭引流的护理,保持稳定的负压状态,观察创面生长状况,加强营养支持。     

VSD修复开放性骨折并软组织缺损创面的效果观察及护理

目的:探讨开放性骨折并软组织缺损创面采用负压封闭引流技术(VSD)的修复效果及护理措施。方法:将87例开放性骨折并软组织缺损患者随机分为两组,负压引流组(n=43)清创后采用VSD修复创面,常规换药组(n=44)清创后采用传统换药方法修复创面,Ⅱ期行中厚皮片植皮或皮瓣移植术封闭创面。对比两组治疗效果。结果:负压引流组感染控制时间、清创至Ⅱ期封闭创面时间短于常规换药组,清创后3d、1周的疼痛VAS评分低于常规换药组,术后植皮/皮瓣成活情况优于常规换药组,差异均具统计学意义(P<0.05)。结论:负压封闭引流是治疗开放性骨折并软组织缺损创面的良好方法,精心护理和有效的VSD管理对创面及早愈合具有重要意义。     

应用负压封闭引流配合腓肠肌皮瓣移植治疗胫骨外露创面的临床效果分析

目的 研究应用负压封闭引流配合腓肠肌皮瓣移植治疗胫骨外露创面的临床效果.方法 本次研究选取的研究对象为2014年5月~2015年5月期间在本院进行治疗的胫骨外露患者,将80例患者简单随机分为两组,各40例.传统换药皮瓣移植术治疗(对照组),负压封闭引流配合腓肠肌皮瓣移植治疗(观察组).对比两组胫骨外露患者的疼痛持续时间、病症愈合时间、感染性皮瓣坏死发生率和愈合率.结果 观察组胫骨外露患者的疼痛持续时间、病症愈合时间、感染性皮瓣坏死发生率及愈合率均优于对照组(P<0.05).结论 治疗胫骨外露创面使用腓肠肌皮瓣移植与负压封闭引流联合的方法有利于减轻患者的痛苦,促进患者的康复,适合临床应用.     

封闭负压引流治疗躯干部Ⅳ期压力性溃疡的疗效及护理体会

目的 探讨封闭负压吸引技术治疗躯干部Ⅳ期压力性溃疡的疗效及护理要点.方法 治疗组2007年6月至2010年6月在我院接受封闭式负压吸引治疗的20例躯干部Ⅳ期压力性溃疡患者.清创后将医用泡沫材料覆盖创面,接负压吸引治疗机,14-20d后行皮瓣手术.对照组为2007年以前应用传统换药方法进行处理的20例同类患者,比较两组患者在手术前治疗时间、换药次数、住院时间、手术后创面愈合情况等方面的差别,总结封闭负压吸引技术的护理观察要点.结果 与传统换药方法相比,治疗组手术前治疗时间、换药次数、住院时间明显减少,皮瓣成活率明显提高,各项结果比较均有显著性差异(P<0.01).结论 封闭式负压吸引技术能控制Ⅳ期压力性溃疡创面的感染情况,充分引流以及刺激创面肉芽组织良好生长,是治疗躯干部Ⅳ期压力性溃疡的简单、安全有效的方法,值得临床推广.维持恒定的负压吸引,引流管的管理和密切的护理观察是护理的关键环节.     

负压封闭引流在手部电烧伤治疗中的临床疗效分析

目的:分析探究负压封闭引流在手部电烧伤治疗的临床疗效.方法:此次研究采取随机盲选法,将2018年3月-2019年5月我院收治的76例手部电烧伤患者划分为2组,各组平均38例;其中,对照组实行常规治疗,观察组实行负压封闭引流后手术治疗,对比2组患者的住院时间、细菌培养结果、皮片创面修复及皮瓣创面修复.结果:观察组住院时间明显短于对照组,观察组细菌培养阴性率、皮片创面修复率、皮瓣创面修复率均明显高于对照组,2组之间的数据存在明显差异性(P<0.05).结论:手部电烧伤患者实行负压封闭引流后手术治疗的效果显著,能明显改善肉芽生长情况,缩短住院时间,大大提高皮片及皮瓣的存活率,值得在临床治疗领域中使用及推广.     

持续负压引流术联合藻酸盐敷料在骨科伤口的应用

目的:探讨持续负压封闭引流技术(VSD)联合新型敷料在骨科各种急慢性伤口治疗中的临床效果及护理进展。方法:对22例存在难愈性伤口的患者采用持续负压引流术和藻酸盐敷料换药治疗。结果:10例患者创面红润逐渐愈合,7例经游离皮瓣修复后治愈,3例经II期缝合治愈,1例经游离植皮加间断缝合后愈合,1例因全身体质较差未愈后行转院治疗。结论:持续负压封闭引流术和藻酸盐敷料的应用中细致的观察和护理,防止负压的中断,保持有效的引流是治疗成功的关键。     

负压封闭引流与软组织缺损Ⅰ期修复的对比观察

目的:观察负压封闭引流技术(VSD)与软组织缺损I期修复的临床应用效果。方法:对40例各种原因引起的软组织缺损的创面分别采用VSD技术(试验组20例)和常规植皮、皮瓣修复等手术治疗(对照组20例),统计两组患者创面愈合时间、换药次数、手术治疗效果。结果:VSD组患者创面愈合时间及换药次数较对照组短(P<0.01),而手术治疗效果较对照组高。结论:VSD比软组织缺损I期修复愈合快且治愈率高。     

负压封闭引流结合腓肠肌肌瓣在难治性膝部创面中的应用

目的 探讨负压封闭引流结合腓肠肌肌瓣在修复难治性膝部创面中的应用效果.方法 选择我院2010年1月—2016年12月收治的12例膝部难治性创面患者,均采用腓肠肌肌瓣结合负压封闭引流方法治疗.结果 12例患者手术肌瓣全部成活,2例植皮局部坏死,经过换药后愈合.术后肌瓣外形及功能较为满意,随访3个月~12个月,效果良好.结论 应用封闭负压引流结合腓肠肌肌瓣治疗膝部难治性创面效果良好,值得临床推广应用.     

真空负压封闭引流结合外固定支架治疗小腿骨折并骨筋膜室综合征21例疗效观察

目的:探讨真空负压封闭引流结合外固定支架在小腿骨折并骨筋膜室综合征患者中的效果分析。方法:选择2018年10月-2019年11月接受真空负压封闭引流结合外固定支架治疗的患者21例作为研究对象,随机数字表分为对照组(n=11)和观察组(n=10)。对照组采取常规治疗,观察组在对照组基础上结合真空负压封闭引流结合外固定支架干预治疗,治疗后对患者效果进行评估,比较2组患者住院时间、换药时间、2期创面修复时间,术后并发症发生率。结果:观察组治疗后住院时间、换药时间、2期创面修复时间均低于对照组(P<0.05);经过治疗后,观察组患者关节肿痛、创面水肿、骨髓炎发生情况均低于对照组患者(P<0.05)。结论:将真空负压封闭引流结合外固定支架用于治疗小腿骨折并骨筋膜室综合征患者,有效减少患者住院时间、换药时间、2期创面修复时间,减少患者治疗过程中的患者关节肿痛、创面水肿、骨髄炎发生情况,值得推广应用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.