乌拉地尔预防颅脑手术麻醉拔管期高血压

目的：观察乌拉地尔用于预防颅脑手术麻醉拔管期高血压应激反应的效应。方法：４０例择期颅脑手术患者,随机分为治疗组与对照组,各２０例。在麻醉清醒期分别静注乌拉地尔０．４ｍｇ／ｋｇ或生理盐水０．０８ｍｇ／ｋｇ。记录注药前,注射后１ｍｉｎ,吸痰时、拔管后１,５ｍｉｎ以及回监护室（ＮＩＣＵ）后的血压和心率的变化。结果 治疗组注药后各时期的血压维持均较平稳,与注药前比较变化无显著差异（Ｐ〉０．０５）;对照组的     

中药固金磨积片抗小鼠肝肿瘤作用的实验病理研究

为进一步观察固金磨积抑制肿瘤细胞增殖的效用及其作用机理,选用小鼠肝癌２２瘤株的瘤细胞悬液接种于ＮＩＨ小鼠右前腋皮下,随机分为５组,用药的３组分别以１．８８ｇ／ｋｇ,１０．２０ｇ／ｋｇ和０．５９２ｇ／ｋｇ的剂进行抑制实验。对肿瘤标本应用大体测量,光镜和电镜为病理形态学方法进行观察。结果显示：固金磨积片对肿瘤殖均有明显的抑制作,并且有剂量依赖性,与对照组比较有显著性差异（Ｐ〈０．０５和Ｐ〈０．００１）     

碱性成纤维细胞生长因子对庆大霉素肾毒性治疗作用的形 …

目的：探讨碱性成纤维细胞生长因子（ｂＦＧＦ）对庆大霉素（ＧＭ）肾毒性的治疗作用。方法：雄性Ｗｉｓｔａｒ大鼠２０只,随机分为实验组和对照组各１０只,两组均按每只１６０ｍｇ／ｋｇ．ｄ＾－１肌注ＧＭ９ｄ;实验组于实验第１０天按２０００Ｕ／ｋｇ．ｄ＾－１肌注ｂＦＧＦ９ｄ,而对照组则注射等量生理盐水,让其自然恢复。所有动物均经心脏灌注固定后取肾皮质作光镜和电镜观察。结果：实验组动物肾小管上皮细胞的恢复和再生     

鸦胆子小鼠膀胱灌注在抗膀胱癌中的作用

目的研究鸦胆子油乳(简称鸦油乳)膀胱灌注抑制膀胱癌的作用. [ HTH〗方法分别以100 g*L-1鸦油乳及0.4 g*L-1丝裂霉素对膀胱癌模型ICR小鼠行膀胱灌注,每周分别1次,共10次后处死,通过光镜及电镜观察组织结构的变化过程. 结果灌注后两组细胞异型性不明显,移行上皮表面癌变征象明显减轻,移行上皮层次减少,鸦油乳细胞质内可见空泡. 结论鸦油乳及丝裂霉素抗癌作用效果相似.     

苯海索和利血平对大鼠脑单胺递质含量的影响

Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ大鼠２８只，随机分为４组：（１）对照组：ｎ＝１０，大鼠腹腔注射（ｉｐ）生理盐水（ＮＳ）２ｍｌ．ｋｇ＾－１；（２）利血平组：ｎ＝６，大鼠ｉｐ苯海索３．０ｍｇ．ｋｇ＾－１，每隔４ｈ给药１次，共５次；（４）苯海索加利血平组：ｎ＝６，大鼠ｉｐ苯海索３．０ｍｇ．ｋｇ＾－１，每隔４ｈ给药１次，共５次，最后一次给药时间同时ｉｐ利血平２．０ｍｇ．ｋｇ＾－１。各组动物均在最后一次给药后１     

萘普生β—环糊精包合物的镇痛抗炎作用

目的：研究萘普生β－环糊精（Ｎａｐ·βＣＤ）包合物的抗炎镇痛效果。方法：取大鼠或小鼠随机分成对照组和实验组,采用足跖肿胀法、扭体反应法和电刺激痛阈值法测定。结果：Ｎａｐ与Ｎａｐ·β－ＣＤ包合物测定值ｔ检验,给药后３０ｍｉｎ小鼠扭体值：２５．０ｍｇ／ｋｇ组Ｐ＜００５,１２．５ｍｇ／ｋｇ组Ｐ＜００５;大鼠痛阈值（给药１５ｈ）：２５．０ｍｇ／ｋｇ组Ｐ＜００５,１２．５ｍｇ／ｋｇ组Ｐ＞００５;大鼠足跖肿胀率（给药１ｈ）：Ｐ＜００１．结论：Ｎａｐ·β－ＣＤ包合物的抗炎镇痛作用优于Ｎａｐ单体。     

碱性成纤维细胞生长因子对庆大霉素肾毒性治疗作用的 …

目的：初步观察碱性成纤维细胞生长因子（ｂＦＧＦ）对庆大霉素（Ｇｅｎｔａｍｉｃｉｎ,ＧＭ）肾毒性的治疗作用。方法：雄性Ｗｉｓｔａｒ大鼠３２只,随机分为对照组、实验组（ＧＭ）中的ＧＭ１组、ＧＭ２组、ＧＭ３组各８只。ＧＭ组按１６０ｍｇ／ｋｇ．ｄ＾－１肌注ＧＭ８ｄ,对照组注身等量生理盐水。于实验第９天宰杀对照组和ＧＭ１组的各８只动物,分别取血做血清尿素氮（ＢＵＮ）和血清肌酐（Ｓｃｒ）检查,以取肾皮质做光镜     

安定对学龄前儿童静脉滴注氯胺酮药代动力学的影响

目的：探讨安定对学龄前儿童滴注氯胺酮的药代动力学影响。方法：１６例３．５～６岁择期手术学龄前儿童，随机分为两组，Ⅰ组为氯胺酮组，Ⅱ组为氯胺酮加安定组。用反向高效液相色谱法测定氯胺酮血药浓度，并计算药代动力学参数。结果：两组房室模型均拟合为二房室开放模型、药代动力学参数：Ⅰ组Ｔ１／２α＝（０．１５±０．０４）ｈ，Ｔ１／２β＝（２．８４±０．４４）ｈ，ＣＬ＝（０．３４±０．０６）Ｌ／ｋｇ·ｈ，Ｖｄ＝（０．６６±０．２９）Ｌ／ｋｇ。Ⅱ组Ｔ１／２α＝（０．１４±０．０６）ｈＴ１／２β＝（３．９２±０．５２）ｈ，ＣＬ＝（０．２６±０．０５）Ｌ／ｋｇ·ｈ，Ｖｄ＝（０．６５±０．０７）Ｌ／ｋｇ。结论：安定使氯胺酮清除半衰期延长，清除率降低     

秋水仙碱拮抗肿瘤坏死因子α的肝毒性作用

目的：探讨秋水仙碱（Ｃｏｌ）于在体条件下对重组人肿瘤坏死因子α（ＴＮＦ－α）所致动物肝毒性的影响，方法：将１８只雄性ＢＡＬＢ／ｃ小鼠随机分为Ａ￣Ｃ３组，腹腔注射Ｄ－氨基半乳糖（ＧａｌＮ）７００ｍｇ／ｋｇ致敏，１ｈ后再次腹腔注射ＴＮＦ－α５０μｇ／ｋｇ诱发急性肝坏死。Ａ组小鼠于ＴＮＦ－α给药前１９和４ｈ分别腹腔汶射生理盐水作为对照，Ｂ和Ｃ组则按相同时间灌服或腹腔注射Ｃｏｌ０．５ｍｇ／ｋｇ。给药８ｈ后     

抗高血压药物对自发性高血压大鼠降压及逆转左室肥厚作用

目的使用４种抗高血压药物治疗自发发性高血压大鼠（ＳＨＲ）探讨血压和左室肥厚之间的关系。方法ＳＨＲ４２只雌性，周龄１６周，随机分为６组，（１）高血压基础对照组，在１６周时处死观察有关指标，（２）高血压对照组，（３）卡托普利组（１００ｍｇ／ｋｇ．ｄ＾－１）（４）培哚普利组（５ｍｇ／ｋｇ．ｄ＾－１）（５）双氢克尿塞组（１００ｍｇ／ｋｇ．ｄ＾－１）（６）普萘洛尔组（４０ｍｇ／ｋｇ．ｄ＾－１）给药１６周，观     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.