共查询到20条相似文献,搜索用时 31 毫秒
1.
Lacombe P Mathews PM Schmidt SD Breidert T Heneka MT Landreth GE Feinstein DL Galea E 《Journal of neuroinflammation》2004,1(1):11
Background
The over-expression of transforming growth factor β-1(TGF-β1) has been reported to cause hydrocephalus, glia activation, and vascular amyloidβ (Aβ) deposition in mouse brains. Since these phenomena partially mimic the cerebral amyloid angiopathy (CAA) concomitant to Alzheimer's disease, the findings in TGF-β1 over-expressing mice prompted the hypothesis that CAA could be caused or enhanced by the abnormal production of TGF-β1. This idea was in accordance with the view that chronic inflammation contributes to Alzheimer's disease, and drew attention to the therapeutic potential of anti-inflammatory drugs for the treatment of Aβ-elicited CAA. We thus studied the effect of anti-inflammatory drug administration in TGF-β1-induced pathology. 相似文献2.
Francesca Crobu Luigi Palumbo Erica Franco Serena Bergerone Sonia Carturan Simonetta Guarrera Simone Frea Gianpaolo Trevi Alberto Piazza Giuseppe Matullo 《BMC medical genetics》2008,9(1):13
Background
Transforming growth factor beta 1 (TGF-β1) gene play an important role in the acute myocardial infarction (AMI), however no investigation has been conducted so far in young AMI patients. 相似文献3.
Hsi-Lung Hsieh Hui-Hsin Wang Wen-Bin Wu Po-Ju Chu Chuen-Mao Yang 《Journal of neuroinflammation》2010,7(1):88
Background
Transforming growth factor-β (TGF-β) and matrix metalloproteinases (MMPs) are the multifunctional factors during diverse physiological and pathological processes including development, wound healing, proliferation, and cancer metastasis. Both TGF-β and MMPs have been shown to play crucial roles in brain pathological changes. Thus, we investigated the molecular mechanisms underlying TGF-β1-induced MMP-9 expression in brain astrocytes. 相似文献4.
Ju Gao Wei-Xian Zhao Fu-Shan Xue Luo-Jing Zhou Shao-qun Xu Ning Ding 《Inflammation research》2010,59(7):501-500
Objective
To assess the effects of propofol treatments at different time points on acute lung injury and on the expression of transforming growth factor (TGF)-β1 and the downstream target of TGF-β1, Smad 2, in the lung tissues in the endotoxic rats. 相似文献5.
The influence of physical exercise on the generation of TGF-β1, PDGF-AA,and VEGF-A in adipose tissue
Czarkowska-Paczek B Zendzian-Piotrowska M Bartlomiejczyk I Przybylski J Gorski J 《European journal of applied physiology》2011,111(5):875-881
Adipose tissue is an important organ that produces and secretes hormones and cytokines, including TGF-β1, PDGF-AA, and VEGF-A.
The goal of the present study was to investigate the influence of a single session of acute exercise, as well as the prolonged
endurance training on the production of TGF-β1, PDGF-AA, and VEGF-A in the subcutaneous white adipose tissue in rats. Rats
were randomly divided into two groups: untrained (UT, n = 30) and trained rats (T, subjected to 6-week endurance training with increasing load, n = 29). Both groups were subjected to an acute exercise session with the same work load. The rats were killed before (UTpre,
Tpre), immediately after (UT0h, T0h), or 3 h (UT3h, T3h) after exercise and adipose tissue samples collected. Growth factor
mRNA was evaluated using RT-PCR; the protein levels were measured before and after training (UTpre and Tpre) using the immunoenzymatic
method. TGF-β1 and PDGF-AA mRNA levels were decreased in the UT3h rats compared to the UTpre rats (P = 0.0001 and P = 0.03, respectively), but the VEGF-A mRNA level remained unchanged in the UT0h and UT3h rats compared to UTpre rats. TGF-β1,
PDGF-AA and VEGF-A mRNA levels were decreased in the T3h rats compared to Tpre (P = 0.0002, P = 0.02, and P = 0.03, respectively). TGF-β1, PDGF-AA and VEGF-A mRNA levels significantly increased in the Tpre rats compared to UTpre
(all P = 0.0002). However, the protein levels remained constant. In conclusion, prolonged physical exercise increases growth factor
mRNA in adipose tissue but not protein levels. 相似文献
6.
Wei-Chao Huang Feng-Chang Yen Feng-Shiun Shie Chih-Ming Pan Young-Ji Shiao Cheng-Ning Yang Fong-Lee Huang Yen-Jen Sung Huey-Jen Tsay 《Journal of neuroinflammation》2010,7(1):28
Background
Overactivated microglia that cluster at neuritic plaques constantly release neurotoxins, which actively contribute to progressive neurodegeneration in Alzheimer's disease (AD). Therefore, attenuating microglial clustering can reduce focal neuroinflammation at neuritic plaques. Previously, we identified CCL5 and CCL2 as prominent chemokines that mediate the chemotaxis of microglia toward beta-amyloid (Aβ)aggregates. Although transforming growth factor-β1 (TGF-β1) has been shown to down-regulate the expression of chemokines in activated microglia, whether TGF-β1 can reduce the chemotaxis of microglia toward neuritic plaques in AD remains unclear. 相似文献7.
Jung-Yoon Choe Hyun-Joo Jung Ki-Yeun Park Yoon-Seup Kum Gwan Gyu Song Dae-Sung Hyun Sung-Hoon Park Seong-Kyu Kim 《Inflammation research》2010,59(3):177-188
Objectives
This study is designed to confirm the anti-fibrotic effect of thalidomide on bleomycin-induced lung fibrosis in a mouse model and to identify whether this anti-fibrotic effect is associated with inhibition of the transforming growth factor-β (TGF-β)-induced extracellular signal-regulated kinase1/2 (ERK1/2). 相似文献8.
Rathbone CR Yamanouchi K Chen XK Nevoret-Bell CJ Rhoads RP Allen RE 《Journal of muscle research and cell motility》2011,32(2):99-109
The regulation of adult skeletal muscle repair and regeneration is largely due to the contribution of resident adult myogenic
precursor cells called satellite cells. The events preceding their participation in muscle repair include activation (exit
from quiescence), proliferation, and differentiation. This study examined the effects of transforming growth factor-beta (TGF-β1)
on satellite cell activation, determined whether TGF-β1 could maintain quiescence in the presence of hepatocyte growth factor
(HGF), and whether the regulation of satellite cell activation with TGF-β1 improves the ability of satellite cells to withstand
oxidative stress. The addition of TGF-β1 during early satellite cell activation (0–48 h) or during the proliferative phase
(48–96 h) maintained and induced satellite cell quiescence, respectively, as determined by myogenic differentiation (MyoD)
protein expression. TGF-β1 also attenuated satellite cell activation when used with HGF. Finally, the role of quiescence in
protecting cells against oxidative stress was examined. TGF-β1 treatment and the low pH satellite cell preparation procedure,
a technique that forestalls spontaneous activation in vitro, both enhanced survival of cultured satellite cells following
hydrogen peroxide treatment. These findings indicate that TGF-β1 is capable of maintaining and inducing satellite cell quiescence
and suggest methods to maintain satellite cell quiescence may improve their transplantation efficiency. 相似文献
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Elbeldi-Ferchiou A Ben Ahmed M Smiti-Khanfir M Houman MH Abdeladhim M Belhadj Hmida N Cerf-Bensussan N Louzir H 《Journal of clinical immunology》2011,31(4):574-583
Background
The mechanisms underlying the loss of self-tolerance in systemic lupus erythematosus (SLE) are incompletely deciphered. TGF-β plays a key role in self-tolerance demonstrated by the onset of a fatal autoimmune syndrome associated with lupus autoantibodies in mice lacking a functional TGF-β receptor. The present work aims to define whether resistance to TGF-β might contribute to the pathogenesis of SLE. 相似文献12.
Stephen D. Thorpe Conor T. Buckley Tatiana Vinardell Fergal J. O’Brien Veronica A. Campbell Daniel J. Kelly 《Annals of biomedical engineering》2010,38(9):2896-2909
The objective of this study was to investigate the hypothesis that the application of dynamic compression following transforming
growth factor-β3 (TGF-β3) induced differentiation will further enhance chondrogenesis of mesenchymal stem cells (MSCs). Porcine
MSCs were encapsulated in agarose hydrogels and cultured in a chemically defined medium with TGF-β3 (10 ng/mL). Dynamic compression
(1 Hz, 10% strain, 1 h/day) was initiated at either day 0 or day 21 and continued until day 42 of culture; with TGF-β3 withdrawn
from some groups at day 21. Biochemical and mechanical properties of the MSC-seeded constructs were evaluated up to day 42.
The application of dynamic compression from day 0 inhibited chondrogenesis of MSCs. This inhibition of chondrogenesis in response
to dynamic compression was not observed if MSC-seeded constructs first underwent 21 days of chondrogenic differentiation in
the presence of TGF-β3. Spatial differences in sGAG accumulation in response to both TGF-β3 stimulation and dynamic compression
were observed within the constructs. sGAG release from the engineered construct into the surrounding culture media was also
dependent on TGF-β3 stimulation, but was not effected by dynamic compression. Continued supplementation with TGF-β3 appeared
to be a more potent chondrogenic stimulus than the application of 1 h of daily dynamic compression following cytokine initiated
differentiation. In the context of cartilage tissue engineering, the results of this study suggest that MSC seeded constructs
should be first allowed to undergo chondrogenesis in vitro prior to implantation in a load bearing environment. 相似文献
13.
Ng KW O'Conor CJ Kugler LE Cook JL Ateshian GA Hung CT 《Annals of biomedical engineering》2011,39(10):2491-2500
The purpose of the presented work is to examine the response of engineered cartilage to a transient, 2-week application of
anabolic growth factors compared to continuous exposure in in vitro culture. Immature bovine chondrocytes were suspended in agarose hydrogel and cultured for 28 days (Study 1) or 42 days (Study
2) in chondrogenic media with TGF-β1, TGF-β3, or IGF-I either added for only the first 14 days in culture or added to the
media for the entire study period. In both studies, there were no statistical differences in tissue mechanical or biochemical
properties between the growth factors on day 14. In Study 1, growth factor removal led to a significant and drastic increase
in Young’s modulus and glycosaminoglycans content compared to continuously exposed controls on day 28. In Study 2, both TGF-β1
and β3 led to significantly higher mechanical properties and collagen content vs. IGF-I on day 42. These results indicate
that the rapid rise in tissue properties (previously observed with TGF-β3 only) is not dependent on the type but rather the
temporal application of the anabolic growth factor. These findings shed light on possible techniques to rapidly develop engineered
cartilage tissue for the future treatment of osteoarthritis. 相似文献
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Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts 总被引:16,自引:0,他引:16
Skutek M van Griensven M Zeichen J Brauer N Bosch U 《European journal of applied physiology》2001,86(1):48-52
The objective of the study was to investigate whether the response profile of the growth factor of human tendon fibroblasts
could be beneficially influenced through the application of mechanical stretch. It was considered that this would elucidate
structural and functional problems, often seen after tendon and ligament healing. The secretion pattern of transforming growth
factor-beta (TGF-β), platelet-derived growth factor (PDGF) and basic fibroblast growth factor (bFGF) was determined in mechanically
stretched fibroblasts and compared to non-stretched controls. Human tendon fibroblasts were experimentally stretched for 15
and 60 min at a frequency of 1 Hz and an amplitude of 5%. The secretion of TGF-β, PDGF and bFGF was measured by enzyme-linked
immuno-sorbent assay. All the growth factors investigated were indeed secreted by human tendon fibroblasts both in stretched
cells and controls. Mechanical stretch increased the secretion pattern of the growth factors. The increased concentrations
of TGF-β, bFGF and PDGF after cyclical mechanical stretching may have a positive influence on tendon and ligament healing
through stimulation of cell proliferation, differentiation and matrix formation.
Electronic Publication 相似文献
16.
Shevach EM Davidson TS Huter EN Dipaolo RA Andersson J 《Journal of clinical immunology》2008,28(6):640-646
Introduction A number of studies have suggested that transforming growth factor beta (TGF-β) plays a critical role in immune suppression
mediated by Foxp3+ regulatory T cells. TGF-β in concert with interleukin 2 is a potent induction factor for the differentiation of Foxp3+ Treg from naive precursors. Polyclonal TGF-β-induced Treg (iTreg) are capable of preventing the autoimmune syndrome that
develops in scurfy mice that lack Foxp3+ Treg. Antigen-specific iTreg can be used to both prevent and treat autoimmune gastritis that is induced by transfer of naive
or primed autoantigen-specific T cells. TGF-β complexed with latency-associated peptide is expressed on the surface of activated
thymus-derived Treg. Coculture of activated Treg with naive responder T cells results in the de novo generation of fully functional
Foxp3+ T cells in a contact-dependent and TGF-β-dependent manner.
Conclusions and Speculations Generation of functional Foxp3+ T cells via this pathway may represent a mechanism by which Treg maintain tolerance and expand their repertoire. 相似文献
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Cansheng Zhu Zhaojun Xiong Xiaohong Chen Zhengqi Lu Guoyu Zhou Dunjing Wang Jian Bao Xueqiang Hu 《Inflammation research》2011,60(8):769-774
Objective
We aimed to investigate the regulation and contribution of vascular endothelial growth factor (VEGF) and sFlt-1(1–3) to human monocytic THP-1 migration. 相似文献19.
Introduction The immune response is controlled by several inhibitory mechanisms. These mechanisms include regulatory T cells, which exist
in multiple classes. Notable among these are Foxp3-expressing regulatory T cells (Treg), NKT cells, and Tr1 cells. Common
to these mechanisms are inhibitory cytokines such as interleukin-10 and transforming growth factor-beta (TGF-β). TGF-β and
Foxp3-expressing Treg cells are critical in maintaining self-tolerance and immune homeostasis.
Discussions The immune suppressive functions of TGF-β and Treg cells are widely acknowledged and extensively studied. Nonetheless, recent
studies revealed the positive roles for TGF-β and Treg cells in shaping the immune system and the inflammatory responses.
In this paper, we will discuss the role of these mechanisms in the control of immunity and autoimmunity and the mechanisms
that underlie how these molecules control these responses. 相似文献
20.
Kristian P Doyle Egle Cekanaviciute Lauren E Mamer Marion S Buckwalter 《Journal of neuroinflammation》2010,7(1):62