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1.
Elvira Fiallo-Olivé Jesús Navas-Castillo Enrique Moriones Yamila Martínez-Zubiaur 《Archives of virology》2010,155(12):2053-2058
Rhynchosia minima is a host for several begomoviruses, both in the Old World and the New World. In Cuba, a whitefly-transmitted disease causing
yellow mosaic symptoms, suggested to be of viral origin, was described more than 30 years ago in R. minima, but no information about the nature of the viruses infecting this weed in this country is available to date. Here, we report
the detection of isolates of two novel begomovirus species infecting R. minima in Cuba, which we proposed be named Rhynchosia golden mosaic Havana virus (RhGMHaV) and Rhynchosia rugose golden mosaic virus
(RhRGMV). The highest nucleotide sequence identities of RhGMHaV and RhRGMV DNA-A were with isolates of Rhynchosia golden mosaic
virus (78.7%) and Sida golden mosaic virus (87.5%), respectively. Phylogenetic analysis demonstrated that these novel viruses
belong to two different lineages of New World begomoviruses. 相似文献
2.
Hernández-Zepeda C Idris AM Carnevali G Brown JK Moreno-Valenzuela OA 《Virus genes》2007,35(2):369-377
Sida acuta and Corchorus siliquosus plants showing yellow mosaic and yellow vein symptoms, respectively, were collected in the Yucatan Peninsula, Mexico. Total
DNA was isolated from both plant species and used for the amplification, cloning, and sequencing of the Begomovirus genome. Nucleotide comparison of the complete DNA-A component isolated from S. acuta and C. siliquosus confirmed the presence of two distinct begomoviruses species. Based on phenotypic symptoms observed in infected field plants,
the names Sida yellow mosaic Yucatan virus (SiYMYuV) and Corchorus yellow vein Yucatan virus (CoYVYuV) were proposed. The
SiYMYuV DNA-A shared the highest nucleotide identity (86%) with the Okra yellow mosaic Mexico virus (OkYMMV). The complete
DNA-B component shared the highest nucleotide identity (80%) with CoYVYuV. The CoYVYuV DNA-A shared the highest nucleotide
identity (84%) with SiYMYuV. The 166-nt common region (CR) sequence for the DNA-A and DNA-B components of SiYMYuV shared a
high nucleotide identity of 99%, and the 151 nt of CoYVYuV CR shared 95% of nucleotide identity. The organization and the
iterated sequence of the putative AC1 binding site (located within the common region) of both isolates, were similar to that
of the begomoviruses of the Western Hemisphere. Phylogenetic analyses placed the DNA-A and DNA-B of SiYMYuV and CoYVYuV in
the clade containing the Abutilon mosaic virus (AbMV). 相似文献
3.
A begomovirus causing mottling and leaf deformation in tomato from the State of Mérida was cloned and sequenced. The virus has a bipartite genome comprised of a DNA-A (2,572 nucleotides) and a DNA-B (2,543 nucleotides) with a genome organization typical of New World begomoviruses. Both components share a common region of 115 nucleotides with 98 % sequence identity. Phylogenetic analysis indicated that while no virus sequences were closely related, the A component was distantly related to those of two other tomato-infecting viruses, tomato leaf deformation virus and Merremia mosaic virus; and the DNA-B, to those of pepper huasteco yellow vein virus and Rhynchosia golden mosaic Yucatan virus. The DNA-A and DNA-B sequences were submitted to GenBank (accession no. AY508993 and AY508994, respectively) and later accepted by the International Committee on Taxonomy of Viruses as the genome of a member of a unique virus species with the name Tomato yellow margin leaf curl virus (TYMLCV). Tomato (Solanum lycopersicum L. ‘Fl. Lanai’) plants inoculated with cloned TYMLCV DNA-A and DNA-B became systemically infected and showed chlorotic margins and leaf curling. The distribution of TYMLCV in tomato-producing states in Venezuela was determined by nucleic acid spot hybridization analysis of 334 tomato leaf samples collected from ten states using a TYMLCV-specific probe and confirmed by PCR and sequencing of the PCR fragment. TYMLCV was detected in samples from the states of Aragua, Guárico, and Mérida, suggesting that TYMLCV is widely distributed in Venezuela. 相似文献
4.
A begomovirus associated with yellow mosaic disease in Rhynchosia minima, a common weed was cloned and sequenced. The virus has a bipartite genome, of which DNA-A is 2727 nucleotide length, and
DNA-B 2679 nucleotides, and has a typical Old World bipartite begomovirus genome organization. Sequence comparison to all
other begomovirus sequences available in the database shows the virus isolated from R. minima to be distinct. Maximum identity of 84% was seen with an isolate of Velvet bean severe mosaic virus-(India: Lucknow:2009)
VBSMV-(IN:Luc:09) (GeneBank Accession No. FN543425), while less than 73% identity was observed with any other legumovirus.
The molecular data show that the virus identified here is a new species in the genus Begomovirus for which the name Rhynchosia
yellow mosaic India virus is proposed. 相似文献
5.
A. M. Collins Malik Mujaddad-ur-Rehman J. K. Brown C. Reddy A. Wang V. Fondong M. E. Roye 《Virus genes》2009,39(3):387-395
Partial genome segments of a begomovirus were previously amplified from Wissadula amplissima exhibiting yellow-mosaic and leaf-curl symptoms in the parish of St. Thomas, Jamaica and this isolate assigned to a tentative
begomovirus species, Wissadula golden mosaic St. Thomas virus. To clone the complete genome of this isolate of Wissadula golden mosaic St. Thomas virus, abutting primers were designed to PCR amplify its full-length DNA-A and DNA-B components. Sequence analysis of the complete
begomovirus genome obtained, confirmed that it belongs to a distinct begomovirus species and this isolate was named Wissadula golden mosaic St. Thomas virus-[Jamaica:Albion:2005] (WGMSTV-[JM:Alb:05]). The genome of WGMSTV-[JM:Alb:05] is organized similar to that of other bipartite
Western Hemisphere begomoviruses. Phylogenetic analyses placed the genome components of WGMSTV-[JM:Alb:05] in the Abutilon mosaic virus clade and showed that the DNA-A component is most closely related to four begomovirus species from Cuba, Tobacco leaf curl Cuba virus, Tobacco leaf rugose virus, Tobacco mottle leaf curl virus, and Tomato yellow distortion leaf virus. The putative Rep-binding-site motif in the common region of WGMSTV-[JM:Alb:05] was observed to be identical to that of Chino del tomate virus-Tomato [Mexico:Sinaloa:1983], Sida yellow mosaic Yucatan virus-[Mexico:Yucatan:2005], and Tomato leaf curl Sinaloa virus-[Nicaragua:Santa Lucia], suggesting that WGMSTV-[JM:Alb:05] is capable of forming viable pseudo-recombinants with these begomoviruses,
but not with other members of the Abutilon mosaic virus clade. Biolistic inoculation of test plant species with partial dimers of the WGMSTV-[JM:Alb:05] DNA-A and DNA-B components
showed that the virus was infectious to Nicotiana benthamiana and W. amplissima and the cultivated species Phaseolus vulgaris (kidney bean) and Lycopersicon esculentum (tomato). Infected W. amplissima plants developed symptoms similar to symptoms observed under field conditions, confirming that this virus is a causal agent
of Wissadula yellow mosaic disease in W. amplissima. 相似文献
6.
The legume yellow mosaic viruses (LYMVs) that cause extensive losses to grain legumes across southern Asia are an evolutionarily unusual group of begomoviruses (genus Begomovirus; family Geminiviridae) with bipartite genomes. All previously identified LYMVs were isolated from leguminous crop species. Here we have identified a virus related to the LYMVs in a common weed, the legume Rhynchosia minima originating from Pakistan. Analysis of the sequence of the virus shows it to be a typical bipartite begomovirus. Sequence comparisons to all other begomovirus sequences available in the databases show the virus from R. minima to be distinct, with the highest level of sequence identity (69.5%) to an isolate of Mungbean yellow mosaic virus. This indicates that the virus identified here is a new species in the genus Begomovirus for which we propose the name Rhynchosia yellow mosaic virus (RhYMV). By Agrobacterium-mediated inoculation we show that, in common with the other LYMVs, the clones of RhYMV are not infectious to the experimental host Nicotiana benthamiana. In soybean, the results of inoculation depended upon the variety. In soybean var. Ig6 the symptoms were mild and plants recovered from infection. However, in var. FS-85, symptoms were severe and progressed to necrosis, indicative of a hypersensitive response. These results indicate that there is resistance to RhYMV in the soybean germplasm. The significance of these results is discussed. 相似文献
7.
Three begomovirus isolates, G46, G83 and G84 from Senecio scandens showing yellow mosaic symptoms were collected from Guangxi Province, P.R. China. The isolates were detected by PCR using universal primers for begomoviruses. Comparison of partial DNA-A sequences (approximately 500 bp) of the isolates revealed their 98.7-99.1% identity. The isolate G46, chosen for complete DNA-A sequencing, consisted of 2746 nt and had a typical genomic organization of begomoviruses. The G46 DNA-A had the highest sequence identity (72.4%) with that of Ageratum leaf curl virus among begomoviruses. The molecular data suggest that the isolate G46 is a new begomovirus (species), for which the name Senecio yellow mosaic virus (Senecio yellow mosaic virus) is proposed. 相似文献
8.
Monopartite begomoviruses comprise DNA-A as the main genome and associated satellite DNAs. Viral DNA extracted from guar (Cyamopsis tetragonoloba) showing leaf curl symptoms exhibited positive amplification of coat protein (CP) gene of DNA-A component, suggesting the
presence of begomovirus. Full length DNA-A was amplified by primer pair re-designed from CP gene nucleotide sequence. The
associated alphasatellite and betasatellite DNA molecules were amplified and sequenced, confirming the presence of monopartite
begomovirus. Sequence comparisons showed 89% identity with other begomoviruses. The Neighbor-Joining tree based on full length DNA-A nucleotide sequence showed that the guar infecting begomovirus clustered
separately from other known begomoviruses. The betasatellite shared a high (96%) nucleotide identity to Cotton leaf curl Multan
betasatellites. The alphasatellite showed 91% nucleotide identity to alphasatellite associated with begomovirus infecting
Okra. Recombination analyses showed three recombinant fragments in DNA-A, two in betasatellite, and four in alphasatellite.
The results suggest that the begomovirus identified in this study was a new recombinant virus. Its name was proposed as Cyamopsis tetragonoloba leaf curl virus (CyTLCuV). 相似文献
9.
Summary. Diseases caused by begomoviruses (family Geminiviridae, genus Begomovirus) constitute a serious constraint to tomato production in Nicaragua. In this study, the complete nucleotide (nt) sequences of the DNA-A and DNA-B components were determined for the first time for Tomato leaf curl Sinaloa virus (ToLCSinV). In addition, the complete nt sequence was determined for the DNA-A component of two isolates of Tomato severe leaf curl virus (ToSLCV). The genome organization of ToLCSinV and ToSLCV was identical to the bipartite genomes of other begomoviruses described from the Americas. A phylogenetic analysis of DNA-A including 45 begomovirus species showed that the indigenous begomoviruses of the New World can be divided into three major clades and an intermediate group: AbMV clade, SLCV clade, “Brazil clade”, and BGYMV group. Phylogenetic analyses of the DNA-A and DNA-B components and their open reading frames indicated that ToLCSinV and ToSLCV belong to different clades: ToLCSinV to the AbMV clade, and ToSLCV to the SLCV clade. The two Nicaraguan isolates of ToSLCV showed a close relationship with ToSLCV from Guatemala (ToSLCV-[GT96-1]) and Tomato chino La Paz virus (ToChLPV), but differed significantly in the AV1 and AC1 regions, respectively. Computer-based predictions indicated that recombination with another begomovirus had taken place within AV1 of ToSLCV dividing this species into two strains. A high probability was also found that ToChLPV is involved in the evolution of ToSLCV. 相似文献
10.
Virus isolate T217L was obtained from a diseased tomato (Solanum lycopersicum) plant showing leaf deformation and chlorotic mottle symptoms near Maracaibo in the state of Zulia, Venezuela. Full-length
DNA-A and DNA-B molecules of T217L were cloned and sequenced. The genome organization of T217L was identical to the bipartite
genomes of other begomoviruses described from the Americas. Characteristic disease symptoms were reproduced in S. lycopersicum and Capsicum annum plants inoculated using the cloned viral DNA-A and DNA-B components, confirming disease aetiology. A sequence analysis of
DNA-A showed that the T217L isolate has the highest sequence identity (84%) with sida yellow mosaic Yucatan virus (SiYMYuV),
sida golden mosaic Honduras virus (SiGMHV) and bean dwarf mosaic virus (BDMV) isolates. This is less than the 89% identity
in the DNA-A component that has been defined as the threshold value for the demarcation of species in the genus Begomovirus. The molecular data show that isolate T217L belongs to a novel tentative begomovirus species, for which the name tomato chlorotic
leaf distortion virus is proposed. TCLDV was also detected in symptomatic C. chinense plants growing near the T217L-infected plant. 相似文献
11.
Yellow mosaic disease was observed on Jatropha gossypifolia plants growing in Kathaupahadi, Madhya Pradesh, India, and whiteflies (Bemisia tabaci) were found in the vicinity. Association of a new begomovirus with yellow mosaic disease of J. gossypifolia has been detected by PCR using begomovirus DNA-A-specific primers. The complete DNA-A genome (~2.7 kb) of this virus isolate
was amplified by rolling-circle amplification (RCA) followed by digestion with Bam HI. The ~2.7-kb amplicons was cloned and sequenced, and the data obtained were submitted to GenBank under accession numbers
FJ177030. The genome of the virus isolate consisted of six open reading frames (ORFs): V2 (pre-coat protein) and V1 (coat
protein) in the virion sense and C3 (REn protein), C2 (TrAP protein), C1 (replication-associated protein) and C4 (C4 protein)
in the complementary sense. BLASTn analysis of the nucleotide sequence (2757 nt) of the viral genome (FJ177030) showed 84–85%
identity and a distinct phylogenetic relationship with DNA-A of tomato leaf curl virus-Bangalore II (U38239) and tomato leaf
curl Karnataka virus (AY754812). Based on its 85% sequence identity to all other begomoviruses known to date and ICTV species
demarcating criteria (< 88% identity), the name Jatropha yellow mosaic India virus (JYMIV) is proposed. JYMIV is considered
to be monopartite, as neither DNA-B nor DNA-β components associated with begomoviruses were detected. 相似文献
12.
Two distinct full-length begomovirus DNA-A components and a DNA-B component were PCR amplified, cloned and sequenced from
Jamaican Malvastrum americanum plants exhibiting yellow mosaic symptoms. Whereas one of the DNA-A components is from a potentially new species that we have
tentatively named Malvastrum yellow mosaic Helshire virus (MaYMHV), the other DNA-A and the DNA-B form a cognate pair and
represent a new virus species tentatively named Malvastrum yellow mosaic Jamaica virus (MaYMJV). The MaYMJV genome components
together infected M. americanum and produced yellow mosaic symptoms similar to those seen in naturally infected plants. Both the MaYMJV and MaYMHV DNA-A
components are typical of those of bipartite begomoviruses from the Western Hemisphere. The DNA-As of MaYMJV and MaYMHV are
most closely related to each other (sharing 84% sequence identity) and cluster phylogenetically with begomoviruses found infecting
malvaceous weeds in Cuba and Florida. The DNA-B component of MaYMJV is most similar to that of Sida golden mosaic virus-[USA:Florida]
(SiGMV-[US:Flo]) and Sida golden mosaic Costa Rica virus-[Costa Rica] (SiGMCRV-[CR]). As with many other geminivirus species,
the genomes of MaYMJV and MaYMHV bear traces of inter-species recombination. 相似文献
13.
Elvira Fiallo-Olivé Dorys T. Chirinos Francis Geraud-Pouey Enrique Moriones Jesús Navas-Castillo 《Archives of virology》2014,159(7):1857-1860
The complete genome of a bipartite begomovirus (genus Begomovirus, family Geminiviridae) infecting Jacquemontia sp. (Convolvulaceae) in Venezuela has been cloned and sequenced. Sequence comparison and phylogenetic analysis have shown that it represents an isolate of a novel species with closest relatives being two New World bipartite begomoviruses that infect Convolvulaceae, Jacquemontia mosaic Yucatan virus and Merremia mosaic virus. The DNA-As of these begomoviruses, however, share only 77.0-78.4 % nucleotide sequence identity with the DNA-A of the isolate described here, for which a recombinant origin is suggested. Based on the symptoms observed in the field, the name Jacquemontia yellow mosaic virus (JacYMV) is proposed for this novel bipartite begomovirus. 相似文献
14.
Fiallo-Olivé E Navas-Castillo J Moriones E Martínez-Zubiaur Y 《Archives of virology》2012,157(1):141-146
As a result of surveys conducted during the last few years to search for wild reservoirs of begomoviruses in Cuba, we detected
a novel bipartite begomovirus, sida yellow mottle virus (SiYMoV), infecting Sida rhombifolia plants. The complete genome sequence was obtained, showing that DNA-A was 2622 nucleotides (nt) in length and that it was
most closely related (87.6% nucleotide identity) to DNA-A of an isolate of sida golden mosaic virus (SiGMV) that infects snap
beans (Phaseolus vulgaris) in Florida. The DNA-B sequence was 2600 nt in length and shared the highest nucleotide identity (75.1%) with corchorus yellow
spot virus (CoYSV). Phylogenetic relationship analysis showed that both DNA components of SiYMoV were grouped in the Abutilon
clade, along with begomoviruses from Florida and the Caribbean islands. We also present here the complete nucleotide sequence
of a novel strain of sida yellow vein virus found infecting Malvastrum coromandelianum and an isolate of euphorbia mosaic virus that was found for the first time infecting Euphorbia heterophylla in Cuba. 相似文献
15.
Fernandes Fernanda R. Cruz A. R. R. Faria J. C. Zerbini F. M. Arag?o Francisco J. L. 《Archives of virology》2009,154(9):1567-1570
We report the complete nucleotide sequences of geminiviruses of the genus Begomovirus infecting soybean (Glycine max) in central Brazil. Samples obtained from soybean plants collected at Santo Antonio de Goiás, Goiás State, showing typical
symptoms of viral infection, were analyzed. Infection was confirmed by PCR-based amplification of a DNA-A fragment with universal
begomovirus primers. Total DNA from infected plants was then subjected to rolling-circle amplification (RCA), and 2.6-kb molecules
were cloned into plasmid vectors. Sequencing of the three DNA-A and two DNA-B clones thus obtained confirmed infection by
three distinct begomoviruses: bean golden mosaic virus, Sida micrantha mosaic virus and okra mottle virus, the last of which
was reported recently to be a novel virus infecting okra plants in Brazil. Begomovirus infection of soybean plants has been
reported sporadically in Brazil and has generally not been considered to be of economic relevance. 相似文献
16.
We report the complete nucleotide sequence of a begomovirus isolate infecting Euphorbia heterophylla and tomato (Solanum lycopersicum) in central Venezuela. Based on the current taxonomic criteria for the genus Begomovirus, the isolate was shown to represent a novel species, tentatively named Euphorbia mosaic Venezuela virus (EuMVV). Its DNA-A
is most closely related to those of Euphorbia-infecting begomoviruses from the Caribbean and Central America. The DNA B component forms a phylogenetic cluster with Euphorbia and Sida-infecting begomoviruses from the squash leaf curl virus (SLCV) cluster. EuMVV is transmissible to S. lycopersicum and Capsicum annuum by biolistics of infectious cloned DNA-A and DNA-B components and induces characteristic leaf downward curling and yellowing
in S. lycopersicum and and yellowing and leaf distortion in Capsicum annuum. 相似文献
17.
Zhou YC Noussourou M Kon T Rojas MR Jiang H Chen LF Gamby K Foster R Gilbertson RL 《Archives of virology》2008,153(4):693-706
Tomato yellow leaf curl (TYLC) and tomato leaf curl (ToLC) diseases are serious constraints to tomato production in Mali and
other countries in West Africa. In 2003 and 2004, samples of tomato showing virus-like symptoms were collected during a survey
of tomato virus diseases in Mali. Three predominant symptom phenotypes were observed: (1) TYLC/ToLC (stunted upright growth
and upcurled leaves with interveinal yellowing and vein purpling), (2) yellow leaf crumple and (3) broccoli or bonsai (severe
stunting and distorted growth). Squash blot (SB) hybridization with a general begomovirus probe and/or SB/PCR analyses revealed
begomovirus infection in plants with each of these symptom phenotypes and no evidence of phytoplasma infection. Sequence analysis
of PCR-amplified begomovirus fragments revealed two putative new begomovirus species associated with the TYLC/ToLC and yellow
leaf crumple symptom phenotypes, respectively. Full-length clones of these begomoviruses were obtained using PCR and overlapping
primers. When introduced into N. benthamiana and tomato plants, these clones induced upward leaf curling and crumpling (the TYLC/ToLC-associated begomovirus) or downward
leaf curl/yellow mottle (yellow leaf crumple-associated begomovirus) symptoms. Thus, these begomoviruses were named tomato
leaf curl Mali virus (ToLCMLV) and tomato yellow leaf crumple virus (ToYLCrV). The genome organization of both viruses was
similar to those of other monopartite begomoviruses. ToLCMLV and ToYLCrV were most closely related to each other and to tobacco
leaf curl Zimbabwe virus (TbLCZV-[ZW]) and tomato curly stunt virus from South Africa (ToCSV-ZA). Thus, these likely represent
tomato-infecting begomoviruses that evolved from indigenous begomoviruses on the African continent. Mixed infections of ToLCMLV
and ToYLCrV in N. benthamiana and tomato plants resulted in more severe symptoms than in plants infected with either virus alone, suggesting a synergistic
interaction. Agroinoculation experiments indicated that both viruses induced symptomatic infections in tomato and tobacco,
whereas neither virus induced disease symptoms in pepper, common bean, small sugar pumpkin, African eggplant, or Arabidopsis. Virus-specific PCR primers were developed for detection of ToLCMLV and ToYLCrV and will be used to further investigate the
distribution and host range of these viruses. 相似文献
18.
Cecilia Hernández-Zepeda Gerardo Argüello-Astorga Ali M. Idris Germán Carnevali Judith K. Brown Oscar A. Moreno-Valenzuela 《Virus genes》2009,39(3):371-374
The complete DNA-A component sequence of Desmodium leaf distortion virus (DeLDV, Begomovirus) isolated in Yucatan was determined to be 2569 nucleotides (nt) in length, and it was most closely related to Cotton leaf crumple virus-California (CLCrV-[Cal]), at 76%. The complete DNA-B component sequence was 2514 nt in length, and shared its highest nucleotide
identity (60%) with Potato yellow mosaic Trinidad virus (PYMTV). Phylogenetic analyses group the DeLDV DNA-A component in the SLCV clade, whereas, the DeLDV DNA-B was grouped with
the Abutilon mosaic virus clade, which also contains PYMV, suggesting that the DeLDV components have distinct evolutionary histories, possibly as the
result of recombination and reassortment. 相似文献
19.
A distinct bipartite begomovirus was found associated with tomato plants showing yellowing, curling, and crumpling of the
leaves, in a sub-temperate region in India. The complete DNA-A and DNA-B components were amplified through rolling circle
amplification (RCA) using Φ-29 DNA polymerase and characterized. The DNA-A of the isolate was comprised of 2,756 nucleotides,
encoding six open reading frames (ORFs) and DNA-B that of 2,725 nucleotides, encoding two ORFs. Genome organization of the
isolate was typical of an old world bipartite begomovirus. Comparisons showed that DNA-A and its intergenic region (IR) have
the highest sequence identity (86% and 84%, respectively) with the Tomato leaf curl New Delhi virus (ToLCNDV; DQ116885) and some other begomoviruses (>84%) reported from cucurbits and tomato. This data suggested that the
isolate is a distinct begomovirus species for which a name Tomato leaf curl Palampur virus (ToLCPMV) is proposed. DNA-B showed the maximum sequence identity (73%) with Tomato leaf curl New Delhi virus-India-[Pakistan:Dargai:T5/6:2001] (AY150305). The common region (CR) of DNA-A and DNA-B showed 94% sequence similarity with
each other. In the present study, phylogenetic relationship of this new species was also established with different begomoviruses
reported from tomato and other begomoviruses showing highest homologies with complete DNA-A and DNA-B sequences. ToLCPMV is
being reported from a sub-temperate region in India which was previously unaffected by begomoviruses and its whitefly vector.
An erratum to this article can be found at 相似文献
20.
D. Pramesh Bikash Mandal Chigurupati Phaneendra V. Muniyappa 《Archives of virology》2013,158(3):531-542
Croton yellow vein mosaic virus (CYVMV) is a widely occurring begomovirus in Croton bonplandianum, a common weed in the Indian subcontinent. In this study, CYVMV (genus Begomovirus, family Geminiviridae) was transmitted by whiteflies (Bemisia tabaci) to as many as 35 plant species belonging to 11 families, including many vegetables, tobacco varieties, ornamentals and weeds. CYVMV produced bright yellow vein symptoms in croton, whereas in all the other host species, the virus produced leaf curl symptoms. CYVMV produced leaf curl in 13 tobacco species and 22 cultivars of Nicotiana tabacum and resembled tobacco leaf curl virus (TobLCV) in host reactions. However, CYVMV was distinguished from TobLCV in four differential hosts, Ageratum conyzoides, C. bonplandianum, Euphorbia geniculata and Sonchus bracyotis. The complete genome sequences of four isolates originating from northern, eastern and southern India revealed that a single species of DNA-A and a betasatellite, croton yellow vein mosaic betasatellite (CroYVMB) were associated with the yellow vein mosaic disease of croton. The sequence identity among the isolates of CYVMV DNA-A and CroYVMB occurring in diverse plant species was 91.8-97.9 % and 83.3-100 %, respectively. The CYVMV DNA-A and CroYVMB generated through rolling-circle amplification of the cloned DNAs produced typical symptoms of yellow vein mosaic and leaf curling in croton and tomato, respectively. The progeny virus from both the croton and tomato plants was transmitted successfully by B. tabaci. The present study establishes the etiology of yellow vein mosaic disease of C. bonplandianum and provides molecular evidence that a weed-infecting monopartite begomovirus causes leaf curl in tomato. 相似文献