EffectsofCompoundSalviaInjectianonLipidPeroxidationAntioxidantEnzymesActivityinPatientswithChronicCorPulmonale

ＥｆｆｅｃｔｓｏｆＣｏｍｐｏｕｎｄＳａｌｖｉａＩｎｊｅｃｔｉａｎｏｎＬｉｐｉｄＰｅｒｏｘｉｄａｔｉｏｎＡｎｔｉｏｘｉｄａｎｔＥｎｚｙｍｅｓＡｃｔｉｖｉｔｙｉｎＰａｔｉｅｎｔｓｗｉｔｈＣｈｒｏｎｉｃＣｏｒＰｕｌｍｏｎａｌｅＥｆｆｅｃｔｓｏｆＣｏｍｐｏｕ...     

愈肝汤对慢性乙肝患者脂质过氧化的影响

目的　探讨愈肝汤对慢性乙肝患者的脂质过氧化作用。方法　 43例慢性乙肝患者服愈肝汤治疗 3mo ,观察其治疗前后超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH -Px)活力和过氧化脂质(LPO)水平的变化 ,并与 38例健康对照组比较。结果　慢性乙肝患者SOD、GSH -Px活力降低 (P <0 .0 5 ) ,LPO水平升高 (P <0 .0 5 ) ;愈肝汤可升高SOD和GSH -Px酶活性 (P <0 .0 1) ,降低LPO水平 (P <0 .0 5 )。结论　愈肝汤对慢性乙肝患者有明显的抗脂质过氧化作用 ,这是其治疗慢性乙型肝炎、减轻肝细胞损伤的重要机理之一。     

川芎嗪注射液治疗慢性阻塞性肺疾病急性发作的疗效观察

目的:探讨川芎嗪注射液配合常规疗法治疗慢性阻塞性肺疾病(COPD)急性发作的疗效及其作用机制。方法:106例COPD急性发作期患者随机分为常规治疗组和联用川芎嗪治疗组;联用川芎嗪治疗组在常规疗法的基础上联用川芎嗪注射液治疗。观察两组临床疗效并检测患者血浆脂质过氧化物(LPO)、全血谷脱甘肤进氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性变化。结果:联用川芎嗪治疗组显效率为70.37%,常规疗法组为50.00%,有显著性差异(P<0.05)。联用川芎嗪治疗组GSH-Px和CAT明显高于常规组,而LPO明显低于常规组。结论:在常规治疗的基础上联用川芎嗪注射液治疗COPD急性发作能明显提高临床疗效,这与川芎嗪注射液能明显降低患者增强的脂质过氧化反应,纠正失衡的抗氧化物酶有关。     

脂质过氧化与冠状动脉粥样硬化性心脏病

对31名心绞痛或陈旧性心肌梗塞的男性冠心病患者及42名健康男性进行血液检测,冠心病患者的血清过氧化脂质(LPO) 含量较健康者显著增高,而血铜,锌,硒含量及谷胱甘肽过氧化物酶(GSH—Px) 和超氧化物岐化酶(SOD) 活力均较健康者显著降低。多因素分析结果,冠心病患者血LPO与GSH-Px,SOD及硒均呈负相关;血硒含量与GSH-Px活力及血铜含量与SOD活力均呈正相关。脂质过氧化增强在冠心病的发生发展过程中可能起着重要的促进作用。     

苯、甲苯对作业女工血中LPO、SOD、GSH-Px含量影响的探讨

本文对接触苯、甲苯的作业女工血清中过氧化脂质(LPO)、红细胞中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)进行了测定分析。其结果为:(1)接触组女工血中LPO、SOD含量均明显高于对照组。说明苯、甲苯使作业女工体内脂质过氧化反应增强。(2)WBC计数与LPO、SOD和GSHPx含量均呈负相关,提示随着脂质过氧化反应增强WBC计数降低。因此,作者认为脂质过氧化反应增强是苯、甲苯导致WBC计数降低的重要因素,LPO和SOD作为判断苯、甲苯对人体危害的早期监测指标具有一定的意义。     

Heroin abuse and nitric oxide, oxidation, peroxidation, lipoperoxidation

To further reveal the risks of heroin abuse to human body, and to determine the injuries of oxidation, peroxidation and lipoperoxidation induced by nitric oxide and other free radicals to heroin abusers, we determined and compared plasma values of lipoperoxides (LPO), nitric oxide (NO), vitamin C (VC), vitamin E (VE), β-carotene (β-CAR) and erythrocyte values of LPO, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in 114 heroin abusers and 100 healthy volunteers. Using linear regression and correlation as well as stepwise regression and correlation, we also analyzed the effect of the abusing duration, and daily abusing quantity on the above-mentioned biochemical parameters in the heroin abusers. The results showed that, compared with the healthy volunteer groups, the average plasma values of LPO, and NO, and the average erythrocyte value of LPO in the heroin abuser group were significantly increased (P＜0.0001), and the average plasma values of VC, VE, and β-CAR and the average erythrocyte values of SOD, CAT, and GSH-Px were significantly decreased (P＜0.0001). Analysis of linear regression and correlation showed that with prolonged heroin abusing and with increased daily quantity in the heroin abusers, the plasma values of LPO, and NO, and the erythrocyte value of LPO were gradually increased (P＜0.001), whereas the plasma values of VC, VE, and β-CAR and the erythrocyte values of SOD, CAT, and GSH-Px were gradually decreased (P＜0.001). Analysis of stepwise regression and correlation indicated that the plasma values of NO, VC and VE were closely correlated with the abusing duration and daily abusing quantity. These results indicate that the balance between oxidation and antioxidation in the heroin abusers was seriously disturbed, and the injuries induced by nitric oxide and other free radicals, through oxidation, peroxidation and lipoperoxidation to the bodies of heroin abusers exacerbated. It is therefore necessary that in abstaining from heroin dependence, the heroin abusers should acquire sufficient quantities of antioxidants such as VC, VE and β-CAR.     

Effects of calcitonin, risedronate, and raloxifene on erythrocyte antioxidant enzyme activity, lipid peroxidation, and nitric oxide in postmenopausal osteoporosis

BACKGROUND: The aims of this study were to compare erythrocyte antioxidant enzyme activities, lipid peroxidation, and nitric oxide levels (NO) in women with postmenopausal osteoporosis (PMO) and non-porotic postmenopausal healthy controls and to assess the relationship between bone mineral density and these oxidant/antioxidant parameters. Additionally, in vivo effects of three different anti-osteoporotic drugs, calcitonin, risedronate and raloxifene, on the erythrocyte oxidant-antioxidant status in women with PMO were also assessed. METHODS: Postmenopausal women aged 40-65 years and without previous diagnosis or treatment for osteoporosis and independent in activities of daily living were included. Bone mineral density was measured at the lumbar spine and proximal femur using DXA. Erythrocyte enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and lipid peroxidation end-product malondialdehyde (MDA) and nitrite/nitrate levels, by product of NO, were assessed. Fifty-nine women with PMO were included (mean age 56.7 years), 44 completed course of therapy and were analyzed. Twenty-two non-porotic healthy women (mean age 55.8 years) were included as controls. RESULTS: Patients had significantly lower CAT and GSH-Px enzyme activity and higher levels of MDA and NO than non-porotic healthy controls. Proximal femur BMD measurements significantly correlated with NO levels. QUALEFFO scores improved in different levels with these short-term treatments. In all treatment groups, erythrocyte MDA levels significantly decreased; moreover, risedronate reduced NO levels and raloxifene enhanced CAT enzyme activity. CONCLUSIONS: Oxidative stress plays an important role in the pathogenesis of PMO. Studied drugs had ultimate effects on reducing lipid peroxidation. Raloxifene also had potent effects in the enhancement of antioxidant defense system.     

老年充血性心力衰竭与氧自由基、脑钠素的关系及川芎嗪干预效果研究

目的观察川芎嗪治疗老年充血性心力衰竭（CHF）的效果及对氧自由基（OFR）和脑钠素（BNP）的影响。方法选择45例正常对照组及90例老年CHF患者,并随机分为常规治疗组和川芎嗪组,两组共治疗4周,对照组及两组患者治疗前后进行血清超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、过氧化脂质（LPO）及BNP水平进行测定,并观察两组CHF病人治疗前后NYHA分级及心功能指标的变化。结果常规治疗组、川芎嗪组治疗前SOD,CAT水平明显低于正常对照组（P〈0．01）;MDA,LPO,BNP明显高于正常对照组（P〈0．01）;川芎嗪组治疗后SOD,CAT水平明显高于治疗前及常规治疗组（P〈0．01）;MDA,LPO,BNP明显低于治疗前及常规治疗组（P〈0．01）;两组CHF病人治疗后NYHA分级及心功能指标均得到显著改善（P〈0．01）,但川芎嗪组较常规治疗组改善更明显（P〈0．01）。结论川芎嗪有明显的抗氧化作用,并能降低BNP水平,从而改善CHF患者的心功能,血浆BNP,SOD,CAT,MDA,LPO浓度为心力衰竭严重程度、判断疗效的敏感指标,值得临床推广和应用。     

Clinical Study on Zhuyu Tongfu (逐瘀通腑) Serial Recipe Combined with Acupuncture and Massotherapy in Treating Hypertensive Cerebral Hemorrhage

Hypertensive cerebral hemorrhage is acommon ail ment that seriouslythreatens hu-man being's life and health,the mortalityand disability is very high.Integrated clini-cal and experi mental study proved that thebasic pathogenesis of this ail ment is intrace-rebral blood stasis,so the principle of acti-vating blood circulation and removing stasisto dispel blood stasis was effective to a cer-tain extent.Since3years before,we haveused Zhuyu Tongfu(逐瘀通腑,ZYTF)Seri-al Recipe combined with acupu…     

Oxidative Stress before and after Operation in Patients with Chronic Cholecystitis Containing Gallstone

ＩＮＴＲＯＤＵＣＴＩＯＮＣｈｒｏｎｉｃｃｈｏｌｅｃｙｓｔｉｔｉｓｃｏｎｔａｉｎｉｎｇｇａｌｌｓｔｏｎｅｉｓａｃｏｍｍｏｎｄｉｓｅａｓｅｏｆｄｉｇｅｓｔｉｖｅｓｙｓｔｅｍ.Ｓｏｍｅａｕｔｈｏｒｓｈａｖｅｒｅｐｏｒｔｅｄｔｈａｔｌｅｖｅｌｓｏｆｎｉｔｒｉｃｏｘｉｄｅ(ＮＯ)ａｎｄｌｉｐｏｐｅｒｏｘｉｄｅｓ(ＬＰＯ)ｉｎｂｌｏｏｄｏｆｐａｔｉｅｎｔｓｗｉｔｈａｃｕｔｅｃｈｏｌｅｃｙｓｔｉｔｉｓａｒｅｍａｒｋｅｄｌｙｉｎｃｒｅａｓｅｄ,ｗｈｉｌｅｔｈｏｓｅｏｆｖｉｔａｍｉｎＣ(ＶＣ)ａｎｄａｃｔｉｖｉｔｉｅｓｏ…     

Abnormal metabolism of nitric oxide, oxidative stress and lipoperoxidative stress in patients with acute viral myocarditis

Objective To investigate metabolic status of nitric oxide (NO) as well as oxidative and li poperoxidative stress, pathological chain reactions of a series of free radicals , and oxidative and lipoperoxidative damages in patients with acute viral myocar ditis (AVM). Methods Using a random paired control design, plasma levels of NO, lipoperoxides (LPO), vitamin C (VC), vitamin E (VE) and β- carotene (β- CAR), erythrocytic level of LPO as well as activities of erythrocytic superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH- Px) were investigated by spectrophotometr ic assays in 60 AVM patients and 60 healthy adult volunteers (HAV). Results Compared with the HAV group, plasma NO, and LPO in plasma and in erythrocyte of the AVM group significantly increased (P=0. 0001), while VC, VE, β- CAR, SO D, CAT and GSH- Px of the AVM group significantly decreased (P=0. 0001). Co mpared with the AVM group before treatment, plasma NO, and LPO in plasma and in erythrocyte of the AVM group post treatment significantly decreased (P&lt;0. 05 ), whereas plasma VC, VE and β- CAR as well as erythrocytic SOD, CAT and GSH- P x of the AVM group post treatment significantly increased (P&lt;0. 05). Conclusion The findings in this study suggested that in the AVM patients the metabolism of NO was disturbed, and the pathological chain reactions of a series of free radic als were severely aggravated, thus produced the oxidative damage and lipoperoxid ative damages. Therefore, we recommend that antioxidants at suitable dosage, su ch as VC, VE, β- CAR and others, should be given to AVM patients daily to allev iate potential oxidative and lipoperoxidative damages in their bodies.     

Effect of puerarin on fibrinolytic activity and lipid peroxide in patients with coronary heart disease

Effect of Puerarin on Fibrinolytic Activity and Lipid Peroxide in Patients with Coronary Heart Disease@陈江斌 @许家俐 @李建军 @黄从新 @唐其柱 @王晋明 @李庚山     

Oxidative stress before and after operation in patients with chronic cholecystitis containing gallstone

To study the relationship of oxidative, antioxidative constituents and antioxidases in blood with chronic cholecystitis containing gallstone, levels of lipoperoxides (LPO), nitric oxide (NO), vitamin C(VC), vitamin E (VE) and beta-carotene (beta-CAR) in plasma as well as level of LPO, activities of superoxide dismulase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in erythrocytes were investigated by spectrophotometric assay in 107 patients with this condition (PCg) and 100 healthy volunteers (HVs). Compared with HVs group, the average value of LPO and NO in plasma and that of LPO in erythrocytes of PCg group were significantly increased (P < 0.0001), while that of VC, VE and beta-CAR in plasma and the average activities of SOD, CAT and GSH-Px in erythrocytes were significantly decreased (P < 0.0001). Linear regression and correlation analysis for 107 preoperative PCg showed that the value of LPO and NO in plasma and that of LPO in erythrocytes of PCg gradually increased (P < 0.0001), representing a significant linear positive correlation. The value of VC, VE and beta-CAR in plasma and that of SOD, CAT and GSH-Px in erythrocytes of PCg gradually decreased (P < 0.0001), representing a significant linear negative correlation. Stepwise regression and correlation analysis for 107 preoperative PCg suggested that the closest correlation was observed between the course of disease and the value of NO and VC in plasma and that of SOD, GSH-Px and LPO in erythrocytes, r = 0.7306, F = 32.1408, P < 0.0001. Compared with the preoperative PCg group, the average value of LPO and NO in plasma and that of LPO in erythrocytes of the postoperative PCg group were significantly decreased (P < 0.0001). Furthermore, the average value of VC in plasma and that of SOD, CAT and GSH-Px in erythrocytes of the postoperative PCg group were significantly increased (P < 0.0001), whereas no significant difference was found between their average value of VE and beta-CAR in plasma. These findings suggested that oxidative stress was an aggravating pathological condition in PCg group. Therefore, we recommend that in treating PCg, antioxidants such as VC, VE, beta-CAR should be given in order to alleviate their potential oxidative damages.     

大黄抗衰老作用的实验研究

目的探讨大黄的抗衰老效果和作用机理．方法３月龄昆明种健康雄性小鼠４０只，随机分为低剂量组、中剂量组、高剂量组、对照组，观察不同浓度的大黄水煎液对小鼠血中过氧化氢酶（ＣＡＴ）、超氧化物歧化酶（ＳＯＤ）、谷胱苷肽过氧化物酶（ＧＳＨ－Ｐｘ）活性及过氧化脂质（ＬＰＯ）含量的影响．结果大黄可增强小鼠血中ＳＯＤ、ＧＳＨ－Ｐｘ活性（Ｐ＜０．０５～０．０１），使ＬＰＯ含量降低（Ｐ＜０．０５～０．０１），其中尤以中、高剂量给药组作；用明显（Ｐ＜０．０１）．结论大黄可能具有延缓衰老的作用．中图分类号Ｒ２８）．Ｊ     

Effect of Yiqi Huoxue herbs on vascular endothelial cells and platelets in patients with chronic cor pulmonale

Objective: To observe the clinical effect of Yiqi Huoxue, YQHX) herbs in treating the patients with chronic cor pulmonale and to explore its mechanism by determining the relationship of oxidation/antioxidation system and how such herbs change on the function of endothelial cells and platelets.Methods: Fifty-eight patients were divided into two groups: conventional therapy group (control group, 28 patients) and convention plus YQHX herbs group (treated group, 30 patients). The control group received conventional management. The treated group were treated with YQHX 150 ml, twice a day, plus the conventional treatment, and the clinical efficacy was recorded. The lipid peroxidation (LPO), Superoxide dismutase (SOD), α-granule membrane protein (GMP140), partial pressure of arterial oxygen (PaO₂), partial pressure of carbon dioxide in artery (PaCO₂) and circulating endothelial cells (CEO were measured respectively before and after treatment, and the relationship between various parameters were analyzed. The results were compared with those of 10 healthy subjects got at the same period.Results: (1) The effective rate and PaO₂ of the treated group was higher than that of the control group and there were no difference in PaCO₂ between the two groups. (2) The levels of LPO, GMP140, CEC in all the patients before therapy were significantly higher than those of the healthy group, and there were marked decrease in the levels of those after treatment (all P<0.01). On the contrary, the levels of SOD in all the patients before therapy were markedly lower than those in the healthy subjects and increased after treatment, P<0.01. (3) The increase of SOD in the treated group was significantly more obvious than that of the control group. In the treated group, the decrease of LPO, GMP140, CEC were markedly more obvious than those in the control group (all P<0. 01). (4) The number of CEC, as well as GMP140, was negatively correlated to PaO₂ (P<0.01) and SOD (P< 0.01), which was positively correlated to LPO (P<0.01). There was a positive correlation between CEC and GMP140 (P<0.05).Conclusion: YQHX herbs in treating chronic cor pulmonale proved to be effective by balancing the oxidation and antioxidation, protecting the pulmonary endothelial cells and activated platelets and helpful in treating respiratory failure.     

Clinical study on Zhuyu Tongfu Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage

Objective: To observe the clinical efficacy and mechanism of Zhuyu Tongfu (ZYTF) Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage (HCH).Methods: One hundred and eighteen patients with hypertensive cerebral hemorrhage, on the basis of conventional Western medicine treatment, were randomly divided into ZYTF combined with acupuncture and massotherapy group (treated group) and simple Western medicine group (control group); the clinical efficacy, neurofunction deficit scoring (NDS) alterations and hematoma absorption rate of both groups were observed, and also the plasma Superoxide dismutase (SOD) activity, plasma lipid peroxidase (LPO) content, erythrocyte glutathion peroxidase (GSH-Px) activity, hematocrit (Ht) and the whole blood viscosity (Va) change were also observed.Results: In the treated group, the clinical efficacy, NDS improvement and hematoma absorption rate were superior to that of the control group; comparison between the two groups after treatment showed that plasma SOD activity and GSH-Px activity got more elevated and plasma LPO content, Ht and Va more lowered in the the treated group than those in the control group.Conclusion: ZYTF combined with acupuncture and massotherapy has better effect, its therapeutic mechanism was possibly correlated to the elevation of plasma SOD activity, GSH-Px activity and lowering of plasma LPO content, Ht and Va. Supported by the Programs Foundation of Cerebrovascular Disease Treated by Integrative Traditional Chinese and Western Medicine of the Key Subject of TCM of Zhejiang Province (No. 2001-18)     

Relationship between Acute Organophosphorus Pesticide Poisoning and Damages Induced by Free Radicals

~~ Relationship between Acute Organophosphorus Pesticide Poisoning and Damages Induced by Free Radicals~~     

黄芪对高脂血症小鼠血脂及脂质过氧化的影响

[目的]探讨黄芪对高脂血症小鼠血脂及脂质过氧化的影响.[方法]取小鼠随机分组,除正常组外的其他组小鼠灌胃给予脂肪乳剂,建立高脂血症小鼠模型,分别灌胃给予不同剂量黄芪组小鼠18.0,9.0,4.5 g/kg黄芪水提取物,连续4周,测定各组小鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)含量,谷胱甘肽过氧化物酶(GSH-Px)和超氧化化物歧化酶(SOD)活性,脂质过氧化物(LPO)含量.[结果]不同剂量黄芪组小鼠血清TC,TG,LDL-C含量明显下降,SOD,GSH-Px活性明显增强,血清LPO含量明显下降.[结论]黄芪具有调节小鼠血脂代谢及增强机体抗脂质过氧化的作用.     

Relationship between Acute Organophosphours Pesticide Poisoning and Damages Induced by Free Radicals

Objective To study the relationship between abnormal reactions of free radicals in bodies of patients with acute organophosphorus pesticide poisoning (AOPP) and damages induced by free radicals. Methods 58 AOPP patients and 58 healthy adult volunteers (HAV) were enrolled in an independent samples control design, in which spectrophotometric methods were used to determine the concentrations of nitric oxide (NO) and lipoperoxides (LPO) in plasma, and LPO in erythrocytes, vitamin C (VC), vitamin E (VE) and b-carotene (b-CAR) in plasma as well as activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and acetylcholinesterase (AChE) in erythrocytes. Results Compared with the average values of every biochemical parameter in the HAV group, the average values of LPO in plasma and in erythrocytes, and NO in plasma in the AOPP group were significantly increased (P=0.000001), while the average values of VC, VE, a-CAR in plasma as well as SOD, CAT, GSH-Px and AChE in erythrocytes in the AOPP group were significantly decreased (P=0.000001). The findings of Pearson product-moment correlation analysis between the value of AChE in erythrocytes and the values of above biochemical parameters for 58 AOPP patients showed that there was a significant linear negative correlation between AChE in erythrocytes and LPO, NO in plasma, and LPO in erythrocytes (P=0.000001～0.001319), while there was a significant linear positive correlation between AChE in erythrocytes and VC, VE, a-CAR in plasma as well as SOD, CAT, GSH-Px in erythrocytes (P=0.000013～0.000824). The results of discriminant analysis of above chemical parameters for 58 AOPP patients and 58 HAV suggested that the correct rates of discriminant analysis were increased to 100 % when the values of AChE and LPO in plasma and in erythrocytes, or AChE and others, were jointly used for the discriminant analysis. Conclusion The findings of the present study suggest that a series of free radical reactions in AOPP patients' bodies are pathologically aggravated, and the discriminant analysis used the above biochemical parameters could markedly increase its correct rates for AOPP patients.     

口腔扁平苔藓在中西药物治疗前后血清SOD和LPO水平的变化

目的　探讨中西药物治疗对口腔扁平苔藓 (OLP)患者血清超氧化物歧化酶 (SOD)和脂质过氧化物 (LPO)水平变化的关系。方法　应用中西药物治疗OLP一疗程 ,观察其疗效 ,并测定治疗前后血清SOD和LPO水平的变化。结果　临床总有效率为 76.19% ,痊愈率为 3 8.10 %。治疗前血清SOD和LPO与正常对照组比较 ,差异有显著性 (P <0 .0 0 1) ;治疗后SOD水平显著升高 ,LOP水平下降明显 ,与正常对照组比较 ,差异无显著性 (P >0 .0 5 )。结论　中西药物治疗OLP临床疗效较好 ,可能与提高SOD水平 ,降低LPO水平有关。