系统性红斑狼疮患者血和尿DNaseⅠ与肌动蛋白的相关性研究

目的探讨系统性红斑狼疮(SLE)患者血和尿中DNaseⅠ活性与肌动蛋白浓度的相关性。方法应用DNA-甲基绿比色法和ELISA间接竞争法检测65例SLE患者和30例正常人对照血、尿中的DNaseⅠ活性和肌动蛋白浓度,分析其相关关系。结果SLE组患者血清、尿DNaseⅠ活性均低于对照组(P<0.01),病情活动期组两者亦均低于缓解期组(P<0.01);肾损害组血清DNaseⅠ活性与非肾损害组比较,差异无显著性,而尿DNaseⅠ活性却降低(P<0.01);SLE组血浆肌动蛋白浓度高于对照组(P<0.05),但尿肌动蛋白浓度两组间差异无显著性;活动期患者血浆肌动蛋白浓度高于缓解期(P<0.01),但尿肌动蛋白浓度两组间差异无显著性;肾损害组血浆肌动蛋白浓度与非肾损害组比较,差异无显著性;但前者尿肌动蛋白浓度却高于后者(P<0.05)。SLE患者血浆肌动蛋白浓度与DNaseⅠ活性之间呈负相关(r=-0.35,P<0.05),而尿肌动蛋白浓度与DNaseⅠ活性之间无相关性(r =-0.23,P>0.05)。结论SLE患者血中DNaseⅠ活性与肌动蛋白浓度之间呈负相关,提示血中肌动蛋白浓度的增高可能是DNaseⅠ活性降低的原因之一。     

系统性红斑狼疮患者外周血单一核细胞IL-4受体水平的研究

目的:探讨白介素(IL)-4受体在系统性红斑狼疮(SLE)发病中的作用及其临床意义。方法:应用逆转录-聚合酶链反应(RT-PCR)检测89例SLE患者和30名健康对照者外周血单一核细胞(PBMC)中IL-4受体mRNA的表达水平;用酶联免疫吸附试验(ELISA)检测血清中IL-4水平。结果:①活动期SLE患者、非活动期SLE患者及健康对照者PBMCIL-4受体表达阳性率为100%。②活动期SLE患者、非活动期SLE患者及健康对照者PBMCIL-4受体mRNA表达水平分别为0.604±0.147、0.400±0.130、0.370±0.070。活动期SLE患者和非活动期SLE患者比较、活动期SLE患者和正常对照者比较差异均有显著性(P=0.000),非活动期SLE患者和正常对照者比较差异无显著性(P=0.351)。③血清中IL-4水平在活动期SLE患者显著高于非活动期SLE患者和正常对照组(P=0.000),非活动期SLE患者显著高于正常对照组(P=0.000),活动期和非活动期SLE患者血清中IL-4水平与PBMCIL-4受体表达水平呈正相关(r=0.622～0.859,P<0.05)。结论:IL-4及其受体的异常表达可能在SLE疾病活动和进展过程中起重要作用。检测SLE患者PBMCIL-4受体的表达水平可作为了解疾病活动性的指标之一。     

系统性红斑狼疮患者外周血单一核细胞白介素6受体水平的研究

目的 探讨白介素6(IL-6)及其受体(IL-6R)在系统性红斑狼疮(SLE)发病中的作用。方法 应用逆转录酶-聚合酶链反应(RT-PCR)检测89例SLE患者和30例正常人外周血单一核细胞(PBMC)中IL-6RmRNA的表达水平,用ELISA法检测血清中IL-6水平。结果 ①活动期SLE患者和非活动期SLE患者及正常人IL-6R阳性表达率为100%。②PBMC中,IL-6RmRNA表达水平:活动期SLE患者为0.902±0.273,非活动期SLE患者为0.519±0.11,正常人为0.573±0.24。活动期组和非活动期组和对照组比较,差异均有统计学意义(P=0.00),非活动期组和对照组比较差异无统计学意义(P=0.289)。③血清中IL-6水平:活动期组为71.89±20.02pg/mL,非活动期组为17.96±6.93pg/mL,对照组为0.035±0.035pg/mL。活动组显著高于非活动组和对照组(P=0.00),非活动组显著高于对照组(P=0.00)。活动期SLE患者和非活动期SLE患者血清中的IL-6水平与PBMC上的IL-6R的表达水平呈正相关(r=0.887和r=0.615,P<0.05)。结论 IL-6及其受体的异常表达可能在SLE疾病活动和发展过程中起重要作用。检测SLE患者PBMC中IL-6受体的表达水平可作为评估疾病活动性的参考指标之一。     

系统性红斑狼疮患者外周血单一核细胞核小体释放水平及其临床意义

目的研究系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)释放核小体的水平变化及其与自身抗体、病情活动度的关系。方法应用ELISA方法检测SLE患者PBMC裂解上清液核小体及其血清自身抗体水平,用SLEDAI积分法评估SLE活动度。结果活动期SLE患者PBMC释放核小体的平均水平为(39.39±25.70)IU/mL,明显高于正常人对照组(11.73±7.87)IU/mL和静止期SLE患者(13.44±8.82)IU/mL,并且与其凋亡率呈显著正相关(r=0.83,P<0.001);SLE患者PBMC的核小体释放水平与SLEDAI积分数、ds鄄DNA抗体水平和补体C3降低水平均呈显著正相关(P值分别<0.01、0.01和0.05)。结论SLE患者PBMC凋亡产生的核小体水平变化与SLE临床病情活动程度密切相关,提示核小体可能是诱导SLE发病的重要抗原之一。     

系统性红斑狼疮患者血清γ干扰素诱导的T细胞α趋化因子检测

目的探讨血清γ干扰素诱导的T细胞α(I-TAC)趋化因子水平与系统性红斑狼疮(SLE)疾病活动的关系及意义。方法采用双抗体夹心ELISA法检测血清I-TAC水平。结果①SLE患者血清I-TAC水平(20.20±6.62ng/mL)明显高于正常人对照(3.13±0.70ng/mL),活动期患者(43.96±14.08ng/mL)高于非活动期患者(2.03±0.46ng/mL);患者血清I-TAC水平与疾病活动指数(r=0.54,t=4.87,P<0.001)、血沉(r=0.47,t=3.80,P<0.001)及ANA对数值(r=0.37,t=3.05,P=0.003)呈正相关,它与补体C3(r=-0.26,t=2.03,P<0.05)呈负相关。②肾损组患者(50.22±16.86ng/mL)血清I-TAC水平明显高于非肾损组患者(7.33±5.11ng/mL)。结论I-TAC可能参与SLE的发病机制,血清I-TAC水平可作为监测疾病活动程度和肾脏损害的一个指标。     

系统性硬皮病患者血浆肾上腺髓质素及内皮素水平的测定

目的:探讨肾上腺髓质素(ADM)和内皮素(ET)在系统性硬皮病(SSc)发病中的作用。方法:采用放免法测定28例SSc患者和20名健康人的血浆ADM和ET-1水平。结果:SSc患者血浆ADM水平为(222.26±48.64)pg/mL,高于正常对照组(89.07±13.00)pg/mL,两组间比较,差异有显著性(P<0.01);SSc伴肺动脉高压者其血浆中ADM水平(254.73±39.36)pg/mL明显高于不伴肺动脉高压者(206.88±45.64)pg/mL,二者之间差异有显著性(P<0.01)。SSc患者血浆ET-1水平为(196.45±65.20)pg/mL,高于正常对照组(54.17±11.70)pg/mL,两组间比较,差异有显著性(P<0.01);SSc伴肺动脉高压者其血浆中ET-1水平(273.19±48.97)pg/mL明显高于不伴肺动脉高压者(160.11±31.17)pg/mL,二者之间差异有显著性(P<0.01)。血浆中ADM和ET-1水平呈正相关(r=0.5148,P<0.01)。结论:ADM和ET与SSc关系密切,可能在SSc发病和防治中具有重要的生理及病理学意义。     

血浆骨桥蛋白水平与系统性红斑狼疮活动性的关系

目的 探讨血浆骨桥蛋白与系统性红斑狼疮(SLE)活动程度的关系。方法 采用ELISA方法检测28例健康人与38例SLE患者血浆骨桥蛋白水平,以分析其与SLE活动性变化关系。结果 SLE患者血浆骨桥蛋白水平(420±203)ng/mL明显高于正常对照组(73±14)ng/mL,P<0.001;SLE蛋白尿组(623±88)ng/mL明显高于非蛋白尿组(288±135)ng/mL,P<0.001;活动组(529±143)ng/mL明显高于非活动组(185±66)ng/mL,P<0.001;糖皮质激素治疗后血浆骨桥蛋白水平(142±22)ng/mL与治疗前水平(556±130)ng/mL相比,差异有统计学意义(P<0.001);关节炎组(489±153)ng/mL与非关节炎组(375±222)ng/mL之间差异无统计学意义,P>0.05。血浆骨桥蛋白水平与SLE DAI呈正相关,r=0.93,P<0.001;与C3呈负相关,r=-0.49,P<0.05;与ANA滴度、血细胞沉降率、C4水平无相关性(P>0.05)。结论 血浆骨桥蛋白水平变化与SLE活动程度、肾脏损害及疾病进展有关。     

CD70 mRNA及其蛋白在SLE患者T淋巴细胞中的表达

目的通过研究SLE患者T淋巴细胞CD70mRNA及其蛋白的表达,探讨CD70在SLE发病机理中的作用。方法用定量RT-PCR方法测定15例活动期、15例非活动期SLE患者和15例正常人对照组外周血T淋巴细胞CD70mRNA转录水平。用流式细胞仪检测各组的CD70+CD4+T淋巴细胞阳性率。结果活动期、非活动期SLE患者及正常对照组T淋巴细胞CD70mRNA转录水平分别为(0.82±0.12),(0.73±0.11)和(0.45±0.09),活动期、非活动期SLE患者明显高于正常对照组,差异有统计学意义(P<0.01),且活动期显著高于非活动期SLE患者,差异有统计学意义(P<0.01)。活动期、非活动期SLE患者及正常人外周血CD4+CD70+T淋巴细胞阳性率分别为(80.30±11.04)%,(66.80±3.98)%和(12.48±3.45)%,活动期、非活动期SLE患者明显高于正常对照组,差异有统计学意义(P<0.01),且活动期显著高于非活动期SLE患者,差异有统计学意义(P<0.01)。结论 CD70的过度表达在SLE的发生发展中起着重要的作用,并且CD70过度表达可以作为SLE疾病活动的一个指标。     

白癜风患者血浆中氧化与抗氧化相关指标的检测

目的通过对白癜风患者及健康对照者血浆中氧化与抗氧化相关指标——超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、维生素E(VitE)及一氧化氮(NO)表达水平的测定,探讨氧化应激在白癜风发病机制及疾病发展中的作用和意义。方法选择60例白癜风患者(患者组)和40名健康志愿者(健康对照组)为研究对象,化学法检测血浆中SOD,CAT,GSH-Px的活性及MDA,VE和NO的含量。结果患者组血浆中MDA含量及SOD活性明显高于健康对照组(P<0.01),而GSH-Px活性明显低于健康对照组(P<0.01);进展期白癜风患者血浆中MDA的含量及SOD的活性明显高于稳定期白癜风组(P<0.01),而GSH-Px活性明显低于稳定期白癜风患者(P<0.01);随MDA含量增加,GSH-Px活性逐渐降低,呈显著负相关关系(r=-0.337,P<0.01),而SOD活性逐渐升高,呈显著正相关关系(r=0.347,P<0.01);而在进展期和稳定期白癜风患者血浆中CAT,VE和NO的含量与健康对照组相比差异无统计学意义(P>0.05)。结论白癜风患者血浆中存在氧化-抗氧化失衡,白癜风的发病及病情活动与氧化应激可能相关。     

系统性硬皮病血浆内皮素水平和肺纤维化的相关性研究

为了解系统性硬皮病(SSc)患者血浆中内皮素水平和肺纤维化之间的关系,对40例SSc患者作了胸部薄层CT扫描,参照CollinsCD评分法评出肺纤维化积分,并测定了血浆内皮素浓度,与40名正常人作了对照。对40例SSc患者的肺纤维化积分和其血浆内皮素值作了相关性分析。结果显示SSc组血浆内皮素浓度(80.09±37.91pg/mL)显著高于正常对照组(55.44±12.21),P<0.001。血内皮素浓度和肺纤维化积分呈显著正相关,r=0.61,P<0.001。结论:SSc患者具有高内皮素血症,与肺纤维化关系密切。     

Leprosy elimination at sub-national level

New strategies for the countries that have already achieved the elimination goal, which includes the great majority of the endemic countries, are needed. There is current concern in these countries about the reduction in the political-technical commitment when the goal is achieved and the possibility of the re-emergence of the disease. A review of the literature on the leprosy post-elimination strategy is done. The proposal to estimate the true prevalence using hidden prevalence based on late diagnosis of the new cases is made. Suggestions are explored for strategies of the work after elimination at national level is attained such as the stratification at the first sub-national level, using estimated true prevalence. It is considered necessary to define strategies for the post-elimination phase with the aim of continuing to the long-term objective of the interruption of transmission and the consequent leprosy eradication.     

Study of serum zinc level in leprosy

Serum Zinc level was estimated in different types of leprosy by 'Dithiazone extraction' method in 75 leprosy patients comprising 15 each of Tuberculoid-Tuberculoid (TT); Borderline Tuberculoid (BT); Borderline Borderline (BB); Borderline Lepromatous (BL) and Lepromatous Lepromatous (LL). These findings were evaluated in comparison to 15 normal subjects serving as controls. Serum zinc level was observed to be significantly low in all types of leprosy except tuberculoid leprosy (TT). No significant difference was observed in serum zinc levels before and after 90 days of dapsone Therapy. The findings of our study are of considerable importance as zinc deficiency can be one of the factors involved in non-specific suppression of cell mediated Immunity (CMI) in lepromatous leprosy.     

Comparison of quality of facial scars after single low‐level laser therapy and combined low‐level with high‐level (PDL 595 nm) laser therapy

The main goal of our study was to compare the quality of resulting facials scar 12 weeks after single and combined laser therapy. Forty‐one children from age 1.5 to 5 years with facial scars after injury participated in the study. Thirty‐one underwent laser therapy, 14 were treated using single low‐level laser therapy (670 nm, fluence 3–5 J/cm^?2), and 17 underwent combined high‐level laser therapy with non‐ablative pulsed dye laser (PDL; 595 nm, spot size 7 mm, delay 0.45 ms or 1.5 ms, fluence 9–11 J/cm^?2, cryogen spray/delay 20/30 ms) and low‐level laser therapy. The control group consisted of 10 untreated children. Before treatment and at week 4, 8, and, 12 the scars were evaluated using the POSAS questionnaire. A statistically significant improvement in scars (between ratings before treatment and 4 weeks after therapy, before treatment and 8 weeks after therapy and before treatment and 12 weeks after therapy) was observed in all parameters in both treatment groups (p < 0.0001). For the HLLT+LLLT group the most significant enhancement in the quality of scars was found for all items and at all evaluations, except pigmentation and pliability. There was no improvement observed in quality of facial scars in the control group.     

Serum interleukin-6 level is reflected in elevated high-sensitivity C-reactive protein level in patients with systemic sclerosis

Systemic sclerosis (SSc) is a systemic connective tissue disease of unknown etiology which presents immunological, vascular and connective tissue abnormalities. Serum interleukin (IL)-6 has been reported to be elevated in patients with SSc. Clinical and laboratory findings affecting the elevated level of high-sensitivity C-reactive protein (hs-CRP) were studied in patients with SSc. Clinical and laboratory findings also included serum IL-6 level. Thirty-nine SSc patients (male : female = 7:32, age 19-84 years, mean 62.6 years) were studied. hs-CRP was measured with a nephelometric assay. Serum IL-6 level was measured by enzyme-linked immunosorbent assay. The distributions of hs-CRP showed that 18 cases (46.2%) were not elevated (<0.07 mg/dL), but 21 cases (53.8%) were (≥0.07 mg/dL). Alkaline phosphatase and IL-6 in SSc patients with elevated hs-CRP (291 ± 95 U/L, 3.23 ± 2.74 pg/mL) were significantly more elevated than those in not elevated patients (221 ± 75 U/L, 1.53 ± 1.12 pg/mL) (P < 0.02, P < 0.01). The correlation between hs-CRP level and IL-6 level in SSc patients was 0.687 (P < 0.001). In conclusion, elevated serum IL-6 levels are reflected in elevated hs-CRP levels in SSc patients.     

Dermatological applications of skin potential level measurements.

Decreasing of the negativity of skin potential level (SPL) on the palmar surface of healthy subjects associated with the process of falling asleep, with a return to increased negativity on awakening, was reported by Nishimura and Nagumo (Ergonomics 1985;28(6):905-913). To clarify the dermatological significance of SPL measurements, the SPL of normal skin at various paired sites in the right upper limb, right lower limb and trunk in healthy volunteers and also at the sites of lesions in patients with psoriasis, herpes zoster and nummular eczema were measured. Fitting curves for the decreasing showed exponential characteristics on the palm and the heel where many sweat glands are involved. SPL differences on other sites did not show the exponential decreasing, but kept almost constant values. The values are negative on normal skin, but positive on lesions sites. We also studied the effects of water content in the horny layer on SPL differences and the biorhythmical changes of SPL differences. We discussed the reasons why these results were obtained. The present study provides the promise of an objective, noninvasive and easy method of testing dermatological conditions.     

异位性皮炎患者血浆环磷酸腺苷水平的测定

根据β-肾上腺能阻断理论、异位性皮炎(AD)息者细胞内环磷酸腺普(cANP)可降低.也有学者认为cAMP降低是由于cAMP-磷酸二醋酶活性增高.体液中cAMP水平在一定程度上可反映细胞内cAMP水平.作者测定57例AD患者的血浆cAMP,并根据皮炎轻重和是否伴有呼吸系统Atopy再行分组,分别与正常人比较,均无统计学差异.     

梅毒血清固定患者白细胞介素水平检测

目的探讨对梅毒血清固定患者白细胞介素(IL)水平检测的临床意义。方法选择2009年1月—2013年1月深圳市第七人民医院收治的35例梅毒血清固定患者为观察组、40例治疗后快速血浆反应素试验(RPR)转阴的患者为对照1组,另选择40名健康体检者作为对照2组,对各组进行血清IL-2、IL-12、IL-4、IL-10水平检测及自然杀伤(NK)细胞水平测定。结果观察组血清IL-2、IL-12水平低于两个对照组(P=0.000),血清IL-4、IL-10水平高于两个对照组(P=0.000~0.040),对照1组血清IL-4、IL-10水平高于对照2组(P=0.043、0.031),对照2组NK细胞所占百分率高于观察组(P=0.000),差异均有统计学意义。结论梅毒血清固定患者存在细胞免疫功能抑制,可能是血清固定形成的原因之一。