HIF-1α、VEGF在银屑病皮损中的表达及意义

目的 探讨低氧诱导因子-1(hypoxia-reducible factor-1,HIF-1)血管内皮生长因子(VEGF)在银屑病发病机制中的作用.方法 采用免疫组化法检测寻常型银屑病患者皮损处HIF-1、VEGF表达情况,并与正常皮肤进行比较分析.结果 寻常型银屑病患者皮损处HIF-1、VEGF的表达明显高于正常对照组(P<0.05),HIF-1与VEGF在银屑病患者皮损中的表达差异有显著正相关性(P<0.05).结论 HIF-1与VEGF在银屑病发病机制中的作可能起重要作用,并且具有协同促进性.     

瘦素、脂联素与TNF-α在子痫前期胎盘中的表达及相关性研究

目的检测子痫前期胎盘中瘦素、脂联素及TNF-α的表达并探讨TNF-α与另两个因子的相关性。方法采用免疫组化方法检测55例子痫前期患者及21例正常对照组胎盘瘦素、脂联素及TNF-α的表达水平。结果重度子痫前期组胎盘瘦素表达水平显著高于正常组(P<0.01),轻度组与正常组无统计学差异(P>0.05);重度组胎盘脂联素表达水平显著低于正常组(P<0.01),轻度组与正常组无统计学差异(P>0.05);子痫前期胎盘TNF-α表达水平显著高于正常组(P<0.05及P<0.01);重度组瘦素与TNF-α成正相关(r=0.536),而正常组与病例组中脂联素与TNF-α均无相关性(r=0.416r、=0.398及r=0.254)。结论脂联素、瘦素及TNF-α参与子痫前期的发病,且TNF-α可调节瘦素的水平,但对脂联素的调节不明显。     

血管内皮生长因子及其可溶性血管内皮生长因子受体-1水平与胎儿出生体重

目的本研究通过对比血管内皮生长因子(VEGF)、可溶性血管内皮生长因子受体-1(sFlt-1)水平差异与新生儿出生体重的关系,以探讨其在胎儿出生体重发生中的作用。方法采用免疫组织化学法检测40例分娩正常出生体重儿组(AGA组)、30例高出生体重儿组(LGA组)及30例低出生体重儿组(SGA组)胎盘组织中VEGF、sFlt-1的表达水平。结果①LGA组胎盘组织中VEGF的表达高于AGA组,sFlt-1的表达水平低于AGA组,差异有统计学意义(χ2=21.17,P<0.01)。SGA组胎盘组织中VEGF的表达低于AGA组,sFlt-1的表达水平高于AGA组,差异有统计学意义(χ2=8.44,P=0.04)。②胎盘组织中VEGF的表达水平与胎儿出生体重呈正相关(r=0.427,P<0.01),胎盘组织中sFlt-1的表达水平与胎儿出生体重呈负相关(r=-0.569,P<0.01)。结论孕妇胎盘组织中VEGF及sFlt-1表达水平的变化可能与胎儿出生体重有关。     

增生型糖尿病视网膜病变患者血清及玻璃体中IL-19和VEGF表达水平的研究

目的探讨增生型糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)患者血清及玻璃体中白细胞介素-19(interleukin-19,IL-19)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达及临床意义。方法选取37例PDR患者和40例孔源性视网膜脱离(rhegmatogenous retinal detachment,RRD)患者分别作为实验组和对照组,采用酶联免疫吸附试验(ELISA)检测2组患者血清和玻璃体液中IL-19、VEGF的含量,PDR组患者检测空腹血糖(fasting blood glucose,FBG)、糖化血红蛋白(glycosylatedhemoglobin,Hb Alc)水平。结果 PDR组患者血清和玻璃体液中的IL-19和VEGF水平均显著高于对照组,差异均有统计学意义(t=8.747、16.437;t=10.417、14.978,均P<0.01),且两者之间呈正相关关系(r=0.394,P<0.05;r=0.574,P<0.01)。PDR组患者玻璃体液中IL-19和VEGF水平均高于血清水平,差异均有统计学意义(均P<0.05)。PDR组玻璃体中VEGF表达水平与FBG、Hb Alc均呈正相关性(r=0.361、0.672,均P<0.05);PDR组患者IL-19表达水平与糖尿病病程、FBG、Hb Alc均无明显相关性(均P>0.05)。结论 IL-19和VEGF在PDR的发病机制中扮演着重要角色;IL-19与VEGF正相关,可能参与了PDR新生血管病变的发生发展,有望为PDR的治疗带来更多的靶点。     

瘦素、脂联素与TNF-α在子痫前期患者血清中的表达及相关性研究

目的检测子痫前期患者血清瘦素、脂联素及TNF-α的表达并探讨TNF-α与瘦素、脂联素的相关性。方法采用酶联免疫吸附法(ELISA)检测44例子痫前期患者及24例正常孕妇血清中瘦素、脂联素及TNF-α的表达水平。结果(1)轻、重度子痫前期患者血清瘦素水平分别为4308.60±378.83pg/ml、4476.39±115.18pg/ml,明显高于对照组4041.97±455.39pg/ml,差异有统计学意义(P<0.05,P<0.01);(2)轻度、重度子痫前期患者血清TNF-α水平分别为73.33±57.91pg/ml及538.50±551.25pg/ml,明显高于对照组62.89±82.85pg/ml,而血清脂联素水平分别为8.06±5.06pg/ml、5.28±1.47pg/ml,明显低于对照组12.73±2.90pg/ml,重度组与对照组之间的差异有统计学意义(P<0.05);(3)重度组瘦素与TNF-α成正相关(r=0.478),脂联素与TNF-α成负相关(r=-0.536),轻度组及对照组中TNF-α与瘦素、脂联素均无相关性。结论瘦素、脂联素及TNF-α参与子痫前期的发病,且TNF-α可以调节瘦素及脂联素的水平。     

脂肪细胞因子与胰岛素抵抗的相关性分析

目的探讨肥胖及糖尿病患者血清脂肪细胞因子即:脂联素、肿瘤坏死因子(TNF)及瘦素水平与发生胰岛素抵抗的关系。方法糖耐量正常者和2型糖尿病患者各60例,其中肥胖受试者60例,体重正常者60例。所有受试者均测其脂联素、TNF-α、瘦素、血糖和血脂的水平以及空腹血清免疫反应性胰岛素(FIRI),并测定血压,腰围臀围比值(WHR)。结果糖尿病组的血清脂联素水平明显低于糖耐量正常组,其值分别为(6·09±3·11)mg/L和(14·12±4·17)mg/L,两者有统计学差异(P<0·001)。血清脂联素水平与BMI、FIRI、WHR和胰岛素抵抗指数(HOMA-IR)均负相关(P<0·01)。血清瘦素水平与BMI、FIRI及HOMA-IR明显正相关(P<0·01)。TNF-α与BMI、WHR、FIRI、HOMA-IR呈显著正相关(P<0·01)。血清脂联素与HDL-C呈正相关,而瘦素和TNF-α与HDL-C呈明显的负相关(P<0·01)。统计学上均具有显著意义。血清脂联素水平与TNF-α及瘦素水平呈明显负相关(分别为r=-0·40,P<0·001;r=-0·40,P<0·001)。结论结果显示,脂肪细胞因子即:脂联素、TNF-α及瘦素水平直接或间接参与正常人和糖尿病患者肥胖及胰岛素抵抗的发生或发展。     

血管生成素-Ⅱ在胃癌中的表达及其意义

目的：研究血管生成素-Ⅱ（Ang-Ⅱ）在胃癌和癌周正常组织中蹬表达及影响其表达的病理因素。方法：采用RT-PCR和免疫组化法检测72例胃癌组织和癌周正常组织中Ang-Ⅱ和血管内皮生长因子（VEGF）的表达。结果：Ang-Ⅱ在胃癌组织中的表达与癌周正常组织相比有显著差异（P<0.01）。在肿瘤组织中，Ang-Ⅱ和VEGF的表达相关（r=0.996，P<0.05）。Ang-Ⅱ的表达与肿瘤的分期（r=0.792，P<0.01）、血管侵犯（r=0.829，P<0.01）相关，而与肿瘤组织的分化类型（r=0.289，P=0.250）、淋巴结转移程度（r=0.290，P=0.259）、浆膜层侵犯（r=0.374，P=0.126）无关。结论：Ang-Ⅱ在胃癌中的表达与VEGF的表达、肿瘤的分期、血管侵犯相关。     

血浆血管内皮生长因子及血管紧张素Ⅱ与糖尿病肾病的关系

目的:检测糖尿病患者血浆血管紧张素Ⅱ(ATⅡ)和血管内皮生长因子(VEGF)浓度变化,并探讨二者之间关系及其在糖尿病肾病发生、发展过程中的作用。方法:98例Ⅱ型糖尿病患者分为两组,43例为糖尿病肾病组(DN),55例为糖尿病非肾病组(NDN)。分别测定各组患者血浆中血管紧张素Ⅱ、血管内皮生长因子、糖化血红蛋白(GHbAlc)及尿微白蛋白(UmALB)的含量。结果:DN组和NDN组患者ATⅡ、VEGF、GHbAlc及UmALB水平均明显高于正常对照组(P＜0.01);DN组ATⅡ、VEGF及UmALB明显高于NDN组(P＜0.01),但是GHbAlc水平在两组之间无显著差异(P＞0.05);各组糖尿病患者血浆ATⅡ和VEGF水平呈显著正相关(r=0.465,P＜0.05),血浆VEGF水平与尿微白蛋白呈高度正相关(r=0.540,P＜0.01)。结论:糖尿病患者血浆ATⅡ和VEGF明显升高,二者之间存在相互作用关系,可能共同参与糖尿病肾病的发生和发展过程。     

肥胖患者氧化应激和脂肪细胞因子变化

目的:研究男性单纯肥胖患者氧化应激(OS)和脂肪细胞因子变化及相互关系.方法:测定42例男性肥胖患者和32例健康非肥胖男性个体的血8-异前列腺素F2α(8-iso-PCF2α)、血浆超氧化物歧化酶(SOD)、血清丙二醛(MDA)、脂联素、瘦素、抵抗素、人肿瘤坏死因子可溶性受体1(TNF-R1)、白介素-1β(IL-1β)、白介素-6(IL-6).结果:肥胖患者8-iso-PGF2α、MDA、瘦素、TNF-RI、IL-1β和IL-6均明显高于正常对照组(P<0.05,P<0.01),脂联素和SOD均明显低于正常对照组(P<0.05,P<0.01),2组抵抗素水平无统计学意义(P>0.05).肥胖组相关分析:8-iso-PGF2α与BMI(r=0.54,P<0.05)呈正相关,与脂联素(r=-0.56,P<0.05)呈负相关,瘦素与体脂(r=-0.53,P<0.05)呈负相关,脂联素与LDL(r=-0.54,P<0.05)和IL-6(r=-0.41,P<0.05)呈负相关.多元逐步回归分析:脂联素和IL-6是影响肥胖患者OS变化的主要因素.结论:男性单纯肥胖患者存在OS和脂肪细胞因子变化,脂肪细胞因子与OS存在明显的相关性.     

急性脑梗死患者血清血管内皮生长因子的变化

目的： 探讨急性脑梗死（ACI）患者血清血管内皮生长因子（VEGF）的变化及其在ACI病理生理过程中的作用。方法: 采用双抗体夹心ABC-ELISA法,于发病72 h内、第7 d及第14 d测定病例组（91例ACI患者）的血清VEGF水平,并与对照组（50例健康体检者）比较,同时比较不同卒中亚型的VEGF表达。结果：病例组在发病72 h内、第7 d及第14 d的血清VEGF水平明显高于对照组（P<0.01）;大面积梗死组各时点血清VEGF水平高于小面积组（P<0.01）;心源性栓塞组血清VEGF水平在第7 d、第14 d高于小动脉闭塞组（P<0.05）,但在入院72 h内无显著差异,大动脉粥样硬化组与其它两个亚组无显著差异。VEGF与CRP（r=0.372, P<0.05）、WBC（r=0.647, P<0.01）、NIHSS评分（r=0.448, P<0.05）之间呈正相关,与2周时的神经功能改善程度没有相关性（r=0.193, P>0.05）。结论：ACI患者血清VEGF水平明显增高,可能参与了脑梗死早期的炎症反应、再灌注损伤以及修复病理变化过程,在一定程度上反映了脑梗死缺血缺氧严重程度及预后。     

Decreased expression levels of L-selectin on subsets of leucocytes and increased serum L-selectin in severe psoriasis

L-selectin is a leucocyte adhesion molecule involved in leucocyte interactions with vascular endothelial cells. Following leucocyte activation L-selectin is endoproteolytically released from the cell surface. To assess whether psoriasis vulgaris results in systemic leucocyte activation, we examined expression levels of L-selectin on subsets of peripheral blood leucocytes from patients with psoriasis (n = 25) and normal control subjects. Serum levels of soluble L-selectin were quantified by ELISA in patients with psoriasis (n = 75), pustulosis palmaris et plantaris, and contact dermatitis, as well as normal control subjects. Psoriasis severity was evaluated by psoriasis area and severity index (PASI). L-selectin expression levels on CD4+ T cells, B cells, monocytes, and neutrophils from patients with severe-type psoriasis (PASI > or = 15) was significantly decreased compared with leucocytes from normal control subjects. Furthermore, L-selectin expression on CD4+ T cells showed good inverse correlation with PASI scores. Monocyte L-selectin expression was restored when the skin lesions of psoriasis were remitted. The frequencies of L-selectin+ CD4+ T cells or L-selectin+ CD8+ T cells from patients with psoriasis were almost normal. Serum L-selectin levels in patients with severe-type psoriasis were significantly higher than those in normal control subjects. These results suggest that subsets of leucocytes may be activated in psoriasis, and that L-selectin expression levels on some leucocyte subsets, especially CD4+ T cells, tend to correlate with disease severity of psoriasis.     

老年冠心病患者血清VEGF、NT-proBNP、cTnT与冠状动脉病变程度的相关性

目的 探讨老年冠心病(coronary atherosclerotic heart disease,CHD)患者血清血管内皮生长因子(vascular endothelial growth factor,VEGF)、氨基端脑肽前体(N-terminal pro-brain natriuretic peptide,NT-proBNP)、肌钙蛋白T(cardial troponin T,cTnT)与冠状动脉病变程度的相关性.方法 选取2015年8月~2016年8月南京医科大学附属淮安第一医院收治的113例老年CHD患者为观察组,同期选取64例体检健康老年人为本研究对照组,采用酶联免疫吸附试验(Enzymes linked immunosorbent assay,ELISA)检测所有受试者血清VEGF、NT-proBNP、cTnT水平,分析三者与老年CHD患者冠状动脉病变程度的相关性.结果 观察组血清VEGF、NT-proBNP、cTnT水平均明显高于对照组,差异有统计学意义(P<0.05);冠状动脉3支血管病变组血清VEGF、NT-proBNP、cTnT水平均明显高于2支及1支血管病变组(P<0.05),2支血管病变组血清VEGF、NT-proBNP、cTnT水平均明显高于1支血管病变组(P<0.05);Gensini积分≥30分组血清VEGF、NT-proBNP、cTnT水平均明显高于Gensini积分<30分组(P<0.05);相关性分析结果可见,血清VEGF、NT-proBNP、cTnT水平与Gensini积分呈显著正相关(r=0.772,0.593,0.634,P<0.05).结论 老年CHD患者血清VEGF、NT-proBNP、cTnT与冠状动脉病变程度相关,且随着冠状动脉病变程度增加呈现明显升高趋势.     

Serum Leptin and Adiponectin Levels in Korean Patients with Psoriasis

Psoriasis is a disorder caused by genetic and immunological factors. Leptin, a peptide hormone secreted predominantly from adipose tissue, regulates energy intake and expenditure, as well as the T-helper response. There have been conflicting reports regarding serum levels of leptin and adiponectin in patients with psoriasis. In the present study, we measured serum levels of leptin and adiponectin in Korean patients with psoriasis. Twenty-four patients with psoriasis and fifteen control subjects were included in the study. Serum leptin and adiponectin levels were determined by an immunometric sandwich enzyme-linked immunosorbent assay (ELISA). The mean serum leptin concentration in patients with psoriasis was higher than in controls, and the difference was statistically significant. In contrast, serum adiponectin levels in patients with psoriasis were significantly decreased compared with healthy controls. Leptin levels in vitamin D-deficient patients were statistically significantly higher than in vitamin D-sufficient patients. Serum adiponectin concentrations showed a negative correlation with body mass index (BMI) and psoriasis area and severity index (PASI) in patients with psoriasis. In conclusion, the present study demonstrated that leptin and adiponectin may play a role in the immunopathogenesis of psoriasis and may be useful biomarkers indicating severity of psoriasis in Korean patients.

Graphical Abstract

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新型pH敏感性材料及其给药系统在现代药剂学中的应用进展

 目的 探讨血管内皮生长因子（VEGF）在结直肠癌组织中的表达及其作为肿瘤新生血管标志的临床价值。 方法 采用免疫人VEGF单克隆抗体及鼠抗人因子Ⅷ相关抗原（F-8 RAg）单克隆抗体，通过免疫组化技术对48例结直肠癌手术切除的癌组织及癌旁正常组织（对照组织）中VEGF和F-8 RAg的表达进行检测。 结果 结直肠癌组织VEGF表达阳性率为62.5%（30/48），对照组为16.7%（8/48），χ²＝64.352，P<0.01。结直肠癌组织F-8 RAg标记的MVD为100.9 ± 16.0，对照组为46.8 ± 11.9，t =18.351，P<0.01。VEGF的表达与大肠癌的淋巴结转移（P<0.01）、远处转移（P<0.05）及临床分期有关（P=0.01）；而F-8 RAg标记的MVD与大肠癌的淋巴结转移、远处转移及临床分期无明显相关（P>0.05）。VEGF在结肠癌组织的表达与F-8 RAg的表达无相关性（P>0.05）。 结论 VEGF在结直肠癌组织新生血管有良好表达，可以作为结直肠癌新生血管有价值的标志     

Body composition and serum levels of adiponectin, vascular endothelial growth factor, and interleukin-6 in patients with rheumatoid arthritis

Aim. To investigate differences in body composition and body mass index (BMI) in patients with rheumatoid arthritis (RA) and their correlations with serum production of adiponectin, interleukin-6 (IL-6), and vascular endothelial growth factor (VEGF). Methods. The study included 83 patients (age 53±5 years) with RA treated with methotrexate. We determined their BMI, fat mass, and fat-free mass using bioimpedance analysis, and serum concentrations of adiponectin, VEGF, and IL-6 using immunoassay analysis. Results. Normal BMI was found in 39 (47%), overweight and obesity in 26 (31%), and underweight in 18 (22%) patients. Concentration of adiponectin was lower in overweight/obese patients than in patients with normal BMI (2.1 [0.8-3.9] μg/mL vs 8.9 (7.2-11.3) μg/mL). In underweight patients, it was moderately increased (12.7 [9.3-14.8] μg/mL) and the correlation between the concentrations of adiponectin and IL-6 was positive (r=0.4; P=0.01). Concentrations of VEGF and IL-6 were increased in all groups with RA. The overweight/obese group showed a negative correlation between the concentrations of adiponectin and VEGF (r=- 0.34; P=0.04), a positive correlation between VEGF concentration and fat mass (r=0.39; P=0.02), and a negative correlation between adiponectin concentration and fat mass (r=- 0.23; P=0.02). Conclusion. Inflammatory and angiogenesis activation was found in RA patients with all types of body composition, but only in those with obesity and overweight there was a direct antagonism between adiponectin and VEGF. Further research is needed to identify possible regimens of metabolic correction in different variations of body composition.     

IL-20在寻常型银屑病患者皮损和PBMC中表达的研究

为了观察IL 2 0在寻常型银屑病患者皮损和外周血单个核细胞 (PBMC )中的表达情况 ,用免疫组化、免疫荧光和RT PCR方法检测IL 2 0在寻常型银屑病患者皮损处和正常人相应部位皮肤及PBMC中的表达。结果发现 ,用上述方法在正常人和银屑病患者皮肤中均不能检测到IL 2 0的表达。RT PCR检测发现 ,IL 2 0在寻常型银屑病患者PBMC中的阳性率为 2 5 % ,在正常人PBMC中的阳性率为 87 5 %。由此得出结论 ,虽然皮肤是IL 2 0作用的靶器官 ,但其本身并不表达IL 2 0。PBMC表达IL 2 0 ,但寻常型银屑病患者PBMC中IL 2 0的表达显著低于正常人 (P <0 0 1)。