白细胞介素-4基因启动子多态性与广西壮族支气管哮喘患儿易感性和血清总免疫球蛋白E水平的相关性

目的研究IL-4基因启动子区域-589C/T和-33C/T位点多态性在广西壮族儿童中的分布及与壮族儿童支气管哮喘(哮喘)易感性及血清总IgE水平的相关性。方法采用聚合酶链反应-限制性片段长度多态性方法对健康儿童102例和哮喘患儿72例IL-4基因-589位点和-33位点进行分析,ELISA方法检测2组血清总IgE水平。采用SPSS 14.0软件进行统计学分析。结果1.IL-4-589位点在健康对照组中基因型分布频率为CC 5.9%、CT 23.5%、TT 70.6%,哮喘组为CC 2.8%、CT 19.4%、TT 77.8%;健康对照组等位基因频率为C 17.6%、T 82.4%,哮喘组为C 12.5%、T 87.5%。2.IL-4-33位点在健康对照组和哮喘组中基因型分布和等位基因频率与IL-4-589位点频率分布一致,连锁不平衡值△=0.978。3.哮喘组与健康对照组之间基因型频率和等位基因频率比较差异均无统计学意义(Pa>0.05)。4.哮喘组血清总IgE水平较健康对照组明显增高(P<0.01);各组不同基因型间血清总IgE水平比较差异均无统计学意义(Pa>0.05);3种相同基因型不同组间比较哮喘组血清总IgE水平均较健康对照组高(Pa<0.05)。结论在广西地区壮族儿童人群中,IL-4基因启动子-589位点和-33位点存在多态性,两位点存在连锁不平衡,其多态性与壮族儿童哮喘的易感性无关联,与血清总IgE水平无相关性。     

原发性肾病综合征与细胞毒性T淋巴细胞相关抗原-4基因启动子区-318位点基因多态性的相关性

目的探讨细胞毒性T淋巴细胞相关抗原-4(CTLA-4)与糖皮质激素(GC)耐药型原发性肾病综合征(PNS)中系膜增生性肾小球肾炎(MsPGN)的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测36例PNS-MsPGN患儿和30例正常对照儿童CTLA-4基因启动子区-318位点基因型。结果肾病组CTLA-4基因启动子区-318位点基因型频率分别为CC型38.9%、TC型61.1%和TT型0,等位基因频率为C等位基因69.4%、T等位基因30.6%。肾病组各基因型及等位基因频率与对照组相比均无显著差异(P均>0.03)。结论CTLA-4基因启动子区-318位点基因C/T双态性同GC耐药型PNS-MsPGN患儿无相关性,提示该基点基因多态性可能不参与GC耐药型PNS-MsPGN的发病机制及耐药机制。     

过敏性哮喘患儿T淋巴细胞CD40L表达和血清IgE相关性分析

过敏性哮喘是青少年常见疾病 ,ＩｇＥ引起的高气道反应是本病的主要特征。国外研究表明 :ＩｇＥ产生需要两个信号 ,第一是细胞因子白细胞介素 4(ＩＬ 4)和白细胞介素 13(ＩＬ 13 ) ,第二是Ｂ淋巴细胞表面ＣＤ4 0 和其在Ｔ淋巴细胞表面的配体ＣＤ4 0 Ｌ结合产生的信号传递[1] ,为此 ,我们检测了过敏性哮喘患儿急性发作时Ｔ淋巴细胞表面ＣＤ4 0 Ｌ与Ｂ淋巴细胞表面ＣＤ4 0 表达以及血清ＩＬ 4、ＩｇＥ水平变化 ,并作相关性分析 ,以了解ＣＤ4 0 Ｌ ＣＤ4 0 在哮喘发作机制中的作用。材料和方法1.检测对象 :患儿系上海中医药大学基础部哮喘专家…     

FcεR I-β基因启动子区多态性与哮喘儿童血清总IgE的关系

目的　探讨IgE高亲和力受体 β链 (FcεRI β)基因启动子区 10 9C/T多态性与儿童哮喘血清总IgE水平的关系。方法　采用聚合酶链式反应 限制性片断长度多态性 (PCR RFLP)法检测了 2 0 0 1年 12月至 2 0 0 3年 2月吉林大学第一临床医院 12 6例哮喘儿童和 87名正常儿童的FcεRI β 10 9C/T基因多态性 ;用酶联免疫法检测血清总IgE水平和空气过敏原。结果　FcεRI β 10 9C/T基因型分布及等位基因频率哮喘组与正常对照组比较 ,差异均无显著性意义 ;哮喘组、对照组过敏原检测阳性率在突变基因型与未突变基因型间比较 ,差异均无显著性意义 ;哮喘组FcεRI β 10 9C/T突变纯合型个体的Log10 IgE水平高于突变杂合型及未突变型个体的水平 (P <0 0 5 )。结论　FcεRI β基因启动子区 10 9C/T不是汉族儿童哮喘的易感基因 ,与过敏原亦无关 ,但突变纯合子TT型与血清总IgE水平升高相关联。     

白细胞介素-4受体基因多态性及IgE水平与哮喘预测指数相关性研究

目的研究新疆维吾尔自治区儿童白细胞介素-4受体(IL-4R)基因多态性及Ig E水平与哮喘预测指数(API)的相关性。方法选取167例API(+)、187例API(-)及203例健康婴幼儿(对照组),应用PCR聚合酶链反应和DNA测序法进行基因分型。同时ELISA法检测三组婴幼儿血清Ig E水平。结果 IL-4R基因Arg551Gln位点各基因型频率分布在API(+)组、API(-)组、对照组三组中差异无统计学意义(P0.05);API(+)组血清Ig E水平明显高于API(-)及对照组(P0.01);API(+)患儿中2岁者血清Ig E水平明显低于≥2岁者(P0.01)。结论 Arg551Gln位点多态性与API结果无相关性,而API阳性与Ig E水平升高存在关联性,年龄≥2岁是API阳性的儿童Ig E水平升高的风险因素。     

白介素-4基因启动子多态性与儿童哮喘关系的研究

目的　探讨白介素 4(IL 4)基因启动子 (C5 90 T)多态性与儿童哮喘发病之间的关系。方法　采用聚合酶链反应 限制性片段长度多态性分析 (PCR RFLP)方法检测 2 7例哮喘儿童、30例健康儿童IL 4基因C5 90 T基因型 ;采用ELISA法检测研究对象血IgE值。结果　哮喘组与健康对照组三种基因型 (CC、TT、CT)在分布上无显著性差异 (P >0 0 5 ) ;哮喘组T等位基因出现的频率为 0 6 48,对照组则为 0 5 83 ,两组比较无显著性差异(P >0 0 5 ) ;哮喘组与对照组三种基因型之间血IgE水平无显著性差异 (P >0 0 5 ) ;IgE升高与IgE正常组间基因多态性亦无显著性差异 (P >0 0 5 )。结论　IL 4基因 (C5 90 T)多态性可能与儿童哮喘发病及IgE水平调节无关。     

温州地区汉族支气管哮喘患儿白三烯受体927T/C位点基因多态性

目的 研究白三烯受体(CysLT1)中927T/C(rs320995)位点基因多态性在温州地区汉族儿童中的分布特征及其对哮喘临床特征的影响.方法 采用DNA测序法检测122例汉族哮喘患儿(哮喘组)和100例汉族健康体检儿童(健康对照组)CysLT1 927T/C位点的基因多态性;采用MS/Paedtric 肺功能仪检测肺功能.结果 1.二组均存在927T/C位点基因多态性,哮喘组CC、CT和TT基因型频率分别为33.6%、18.9%和47.5%,健康对照组分别为19.0%、17.0%、63.0%,二组基因型频率分布比较差异有统计学意义(χ2=6.769,P=0.034);哮喘组男童基因型与健康对照组比较差异有统计学意义(χ2=17.776,P=0.000),哮喘组女童基因型与健康对照组比较差异亦有统计学意义(χ2=9.676,P=0.008).2.哮喘组C、T等位基因分别为43.0%和57.0%,健康对照组分别为28.0%和72.0%,二组比较差异有统计学意义(χ2=10.747,P=0.001).哮喘组男童等位基因与健康对照组比较差异有统计学意义(χ2=16.396,P=0.000),哮喘组女童等位基因与健康对照组比较,差异无统计学意义(χ2=0.015,P=0.902).3.哮喘组中,927T/C位点3种基因型特应性体质、家族特应性及哮喘严重程度比较,差异均无统计学意义.结论 1.温州地区汉族儿童CysLT1基因927T/C位点基因多态性与温州地区汉族人群哮喘易感性相关,且在性别上无明显差异.2.该位点C等位基因的男童携带者患哮喘的危险性为T等位基因携带者的2.755倍,但哮喘患病率与C等位基因的女性携带者无明显相关.3.温州地区汉族儿童中CysLT1基因927T/C位点基因多态性与特应性体质、家族特应性及哮喘严重程度无明显相关.     

湖南汉族儿童T淋巴细胞受体保守域α链基因-560C/T多态性与过敏性紫癜发病之间的关系

目的 探讨T淋巴细胞受体保守域α链基因(TCRCα)-560 C/T多态性与过敏性紫癜(HSP)发病之间的关系.方法 收集2010年9月至2011年11月长沙市中心医院、中南大学湘雅三医院、中南大学湘雅二医院就诊的HSP患儿36例,以同期体检的60例健康儿童为健康对照组,应用聚合酶链反应-限制性内切酶片段长度多态分析方法结合琼脂糖凝胶电泳技术,对2组儿童行TCRCα-560 C/T基因多态性分析.结果 TCRCα-560位点的CC、CT、TT3种基因型频率在HSP组和健康对照组间比较差异无统计学意义(x2=5.39,P＞0.05),而该位点的等位基因频率在HSP组和健康对照组间差异有统计学意义(x2=4.69,P＜0.05).结论TCRCα-560C/T基因多态性变化与HSP的发生可能存在一定关联.     

支气管哮喘患儿外周血T淋巴细胞趋化功能与总IgE的变化

目的：探讨支气管哮喘患儿的Ｔ淋巴细胞趋化功能及总ＩｇＥ的变化。方法：应用微量玻片法和放射免疫法，对３２例支气管哮喘发作期患儿和３４例正常对照组进行了检测。结果：支气管哮喘患儿的Ｔ淋巴细胞趋化值（８２７４１±０４２１０）％；总ＩｇＥ（７５４８２２３±１９７８８２４）ｍｇ／Ｌ，明显高于正常对照组的（０４８０７±００４１０）％和（２０１４９２±４８２５９４）ｍｇ／Ｌ。结论：支气管哮喘患儿存在明显的免疫功能紊乱。其表现为Ｔ淋巴细胞分泌免疫球蛋白ＩｇＥ明显增高。Ｔ淋巴细胞趋化值可作为探讨细胞免疫功能的又一新方法。     

咳嗽变异性哮喘患儿血清总IgE检测及分析

目的探讨血清总IgE与咳嗽变异性哮喘的关系,为临床防治提供依据。方法采用酶联免疫法测血清总IgE含量。结果58例咳嗽变异性哮喘患儿中血清总IgE升高占93.1%,32例正常小儿血清总IgE升高占25%,2组比较差异显著(P<0.01)。结论IgE在咳嗽变异性哮喘的发病机制中可能起重要的作用。     

Relationship between cytotoxic T‐lymphocyte antigen 4 ‐318C/T (rs5742909) gene polymorphism and the risk of acute rejection in renal transplantation

Results on the relationship between CTLA4 ‐318C/T (rs5742909) gene polymorphism and risk of acute rejection in renal transplantation are still conflicting. This meta‐analysis was performed to update the association between CTLA4 ‐318C/T and risk of acute rejection in renal transplantation. The association investigations were identified from PubMed and Cochrane Library, and eligible studies were included and synthesized using meta‐analysis method. Twelve reports were included in this meta‐analysis for the association of CTLA4 ‐318C/T gene polymorphism with acute rejection risk in renal transplantation, consisting of 728 acute rejection patients and 1628 non‐acute rejection controls. The association between CTLA4 ‐318C/T gene polymorphism and acute rejection risk in renal transplantation for overall populations was not found in this meta‐analysis (T allele: OR=0.96, 95% CI: 0.60‐1.54, P=.88; TT genotype: OR=0.90, 95% CI: 0.47‐1.71, P=.74; CC genotype: OR=1.00, 95% CI: 0.62‐1.59, P=.98). Interestingly, T allele was associated with the risk of acute rejection in renal transplantation in African population. In conclusion, CTLA4 ‐318C/T gene polymorphism is not associated with the risk of acute rejection in renal transplantation in overall populations.     

协同刺激分子B7和细胞毒T细胞抗原-4与临床疾病

未致敏T细胞的活化至少需要两种信号,只有在两种信号同时存在时,T细胞才能有效活化、增殖,进而分泌细胞因子或发挥细胞毒性作用.第一信号由T细胞受体识别抗原产生,经CD3分子将信号传导至细胞内;第二信号是非抗原依赖性的“协同刺激信号”,它是由抗原呈递细胞或靶细胞表面的协同刺激分子与T细胞表面的协同刺激分子受体相互作用而提供的.这些协同刺激分子中最重要的是抗原呈递细胞表面的B7分子和T细胞表面的CD28、细胞毒T细胞抗原-4.     

CTLA-4 expression in T cells of patients with atopic dermatitis

Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4; CD152) is a surface molecule of activated T cells with sequence homologous to CD28, and may act as a negative regulator of T-cell activation. In murine animal models, cross-linkage of CTLA-4 molecules on the cell surface results in decreased T-cell proliferation, accompanied by increased interleukin (IL)-2 production and apoptosis. To clarify the activation of peripheral blood T cells, we studied the CTLA-4 expression in 32 patients with atopic dermatitis who visited our institution, and 19 normal children who visited for pre-operative laboratory examination were used as normal controls. Whole blood was obtained from all subjects and stained with anti-CD3, anti-CD4, anti-CD8 monoclonal antibodies (mAb). After erythrocyte lysis with lysing solution, the cells were stained with anti-CTLA-4 mAb, and stained cells were analysed by fluorescence-activated cell sorter (FACScan) flow cytometer. Intracellular expression of CTLA-4 was significantly upregulated in peripheral blood CD3+ T cells (36.8%), CD4+ T cells (21.7%) and CD8+ T cells (18.7%) of patients with atopic dermatitis, compared with normal control (18.3%, 9.7%, 9.8%; respectively). Furthermore, CTLA-4-positive CD3+ T cells in patients with severe atopic dermatitis were significantly higher compared with milder group (42.8% vs. 32.2%). However, no significant difference was obtained in CD4+ and CD8+ T cells. Mean percentage of T cells expressing CTLA-4 in patients with atopic dermatitis was higher than the control group. These observations suggest the possibility that the disease activity can be correlated with the CTLA-4 level.     

Association of cytotoxic T‐lymphocyte antigen 4 +49A/G gene polymorphism with acute rejection risk in renal transplantation

The conclusions on the association between cytotoxic T‐lymphocyte antigen 4 (CTLA4) +49A/G gene polymorphism and acute rejection risk in renal transplantation are still debated. This meta‐analysis was performed to update the association between CTLA4 +49A/G and acute rejection risk in renal transplantation. The association investigations were identified from PubMed and Cochrane Library, and eligible studies were included and synthesized using meta‐analysis method. Fourteen reports were included into this meta‐analysis for the association of CTLA4 A/G gene polymorphism and acute rejection risk in renal transplantation, consisting of 962 acute rejection patients and 2084 non‐acute rejection controls. The association between CTLA4 G allele/GG genotype and acute rejection risk in renal transplantation was found in this meta‐analysis (G allele: OR=1.21, 95% CI: 1.03‐1.44, P=.02; GG genotype: OR=1.37, 95% CI: 1.10‐1.69, P=.004). However, the AA genotype was not associated with acute rejection risk in renal transplantation. In conclusion, CTLA4 G allele/GG genotype is associated with the acute rejection risk in renal transplantation.     

Gender-limited association of cytotoxic T-lymphocyte antigen-4 (CTLA-4) polymorphism with cord blood IgE levels

Allergic mechanism has long been attributed to IgE-mediated reaction. The relationship between gene polymorphism and cord blood IgE (CB IgE) is unclear. We investigated whether elevation of CB IgE levels was associated with polymorphisms of cytotoxic T-lymphocyte antigen 4 (CTLA-4) at (−318) CT and (+49) AG positions in a gender-limited fashion. CB IgE levels were determined by Pharmacia CAP system and the CTLA-4 polymorphisms at (−318) and (+49) were determined by restriction fragment length polymorphism (RFLP). A total of 644 consecutive umbilical cord bloods were collected for this study. 32.9% of newborn infants had detectable IgE levels (≥0.35 kU/l). 25.6% of the male newborns had elevated CB IgE levels (≥0.5 kU/l) similar to those of the female newborns (22.7%). The CTLA-4 polymorphism at (+49) but not (−318) was significantly associated with elevated CB IgE levels (p = 0.004). The association of CTLA-4 (+49) A allele with elevated CB IgE levels was found only in females. Both male and female infants with different CTLA-4 (−318) genotypes had no difference in the rates of elevated CB IgE levels. A linkage disequilibrium between CTLA-4 (+49) G and (−318) C allele was found in this Chinese population. Subjects with the (+49, GG and −318, CC) genotype had a significantly lower rate of elevated CB IgE levels. Association of the CTLA-4 (+49) polymorphism with elevated CB IgE levels was found only in female infants. Newborn infants with the (+49, GG and −318, CC) genotype tended to have a low rate of elevated CB IgE.     

T 淋巴细胞毒相关抗原-４基因49位点多态性与儿童1型糖尿病相关性的Meta分析

目的：评价T 淋巴细胞毒相关抗原-4（CTLA-4）基因49位点多态性与儿童1型糖尿病（T1DM）的相关性。方法：检索PubMed、EBSCO、中国学术期刊全文数据库（CNKI）、中国生物医学文献数据库（CBM）、万方数据库,收集CTLA-4基因49位点多态性与儿童T1DM相关性的文献。用Meta分析的方法检测CTLA-4基因49位点的基因型和等位基因在儿童T1DM组与对照组中是否有差异。结果：共纳入10篇文献,共有T1DM 1084例,对照组1338例。根据各项研究的异质性检验结果,均利用固定效应模型对CTLA-4基因49位点的AG、GG、GG+AG基因型和G等位基因与T1DM相关性进行Meta分析,各项研究的合并OR值（95% CI）分别为1.13（0.97～1.33）、1.42（1.16～1.75）、1.20（1.03～1.40）、1.21（1.09～1.33）,提示T1DM组CTLA-4基因49位点G等位基因和GG、GG+AG基因型表达与对照组比较均有显著差异。结论：CTLA-4基因49位点G等位基因和GG、GG+AG基因型均与儿童T1DM的发生相关。     

白介素-4基因C-33T多态性与呼吸道合胞病毒毛细支气管炎的相关性

目的 研究白介素-4(IL-4)基因C-33T与呼吸道合胞病毒(RSV)毛细支气管炎的易感性、病情严重程度的关系及对血清总IgE和鼻咽分泌物(NPS)IL-4水平的影响.方法 采用聚合酶链-限制性片段长度多态法(PCR-RFLP)检测130例RSV毛细支气管炎患儿和108例对照组儿童IL-4/C-33T位点多态性,分别用化学发光法和酶联免疫分析法,检测RSV毛细支气管炎患儿血清总IgE和NPs中IL-4水平.结果 两组IL-4启动子区C-33T位点均可见TT、CT和CC 3种基因型,其中病例组,TT、CT和CC基因型频率分别为66.9%、26.9%和6.2%,对照组分别为69.4%、26.9%和3.7%,两组差异无统计学意义(X2=0.758,P>0.05);病例组T、C等位基因频率分别为80.3%、19.7%,对照组分别为82.9%、17.1%,两组差异亦无统计学意义(X2=0.073,P>0.05;OR=0.847,P>0.05).病例组三种基因型间NPS中IL-4及血清总IgE水平差异均无统计学意义(H=0.103,F=0.529,P均>0.05);三种基因型频率在轻度组和中重度组间的差异亦无统计学意义(X2=0.825,P>0.05).结论 温州地区儿童存在IL-4/C-33T位点的多态性,但未发现其与RSV毛细支气管炎存在关联.     

MTHFR基因多态性与儿童支气管哮喘易感性及糖皮质激素疗效的相关性

目的 探讨亚甲基四氢叶酸还原酶（methylenetetrahydrofolate reductase,MTHFR）基因多态性与儿童支气管哮喘易感性及糖皮质激素（glucocorticoid,GC）疗效的相关性。 方法 选取2018年6月至2020年12月住院治疗的儿童支气管哮喘患儿173例为观察组,均接受GC雾化吸入治疗,连续3个月。选取同期体检的健康儿童178例为对照组。采用PCR检测两组受试儿MTHFR基因C677T位点的基因型,分析两组基因型分布差异性;比较观察组不同基因型患儿治疗前后血清免疫球蛋白E、白细胞介素-8（interleukin-8,IL-8）、白三烯B4（leukotriene B4,LTB4）水平,肺功能指标差异及临床疗效差异。 结果 与对照组相比,观察组TT基因型及T等位基因频率均显著升高（P<0.001）;TT/CT基因型及T等位基因是支气管哮喘易感性的独立危险因素（OR分别为6.615、7.055,P<0.001）。GC治疗后3种基因型患儿免疫球蛋白E、IL-8和LTB4水平较治疗前显著降低,第1秒用力呼气容积（forced expiratory volume in 1 second,FEV1）、用力肺活量（forced vital capacity,FVC）、FEV1/FVC%较治疗前显著升高（P<0.001）;TT基因型患儿IL-8和LTB4水平显著低于CC基因型患儿,LTB4水平明显低于CT基因型患儿,TT基因型患儿FVC明显高于CT基因型患儿,FEV1/FVC%显著高于CC基因型患儿（P<0.05）;治疗后3种基因型患儿临床GC治疗疗效比较差异有统计学意义,其中TT基因型患儿GC疗效良好比例显著高于CC基因型患儿（P<0.05）,且TT基因型是GC疗效良好的独立影响因素（OR=2.111,P=0.018）。 结论 MTHFR基因多态性与儿童哮喘易感性及GC疗效相关,携带TT/CT基因型儿童支气管哮喘发病风险更高,TT基因型对GC治疗具有更高的敏感性。     

辅助性T淋巴细胞1/辅助性T淋巴细胞2平衡与慢性乙型肝炎的关系

目的探讨辅助性T淋巴细胞(Th)1/Th2细胞平衡与慢性乙型肝炎(CHB)的关系。方法CHB患儿42例分别于肝炎活动期及缓解期采空腹血5 mL,双抗体夹心酶联免疫吸附法(ELISA)测定其Th1、Th2细胞分泌细胞因子干扰素-γ(IFNγ-)、肿瘤坏死因子-α(TNFα-)和白细胞介素-10(IL-10)水平。荧光定量PCR法测定其HBV DNA。15例健康对照检测相同指标。结果肝炎活动期患儿IFNγ-、TNFα-较缓解期明显增高(Pa<0.01),缓解期则低于健康对照组(P<0.01,0.05)。在肝炎患儿活动期及缓解期IL-10无差异(P>0.05),但均高于健康对照组(P<0.01,0.05)。黄疸型与无黄疸型肝炎在活动期与缓解期患儿IFNγ-、TNFα-与IL-10均无差异(Pa>0.05)。HBV DNA与IFNγ-、TNFα-及IL-10均无相关性(P>0.05)。上述细胞因子与HBV DNA是否存在前C区1 896位变异无关(P>0.05)。结论CHB患儿存在Th1/Th2细胞失平衡。CHB患儿Th1细胞因子分泌增加可致肝脏炎性反应。在疾病过程中,Th2细胞因子占主导地位,削弱机体对病毒细胞免疫功能,造成病毒持续感染,疾病慢性化。检测Th1/Th2细胞因子变化作为监测CHB患儿疾病病情以及判断预后指标。     

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??Objective To study the change of plasma fibrinogen level and the molecular activity of fibrinogen in children with simple obesity. To explor the relationship between the simple obesity and fibrinogen B??-148C/T gene polymorphism in children and to provide basis for children's simple obesity prevention. Methods A total of 106 children with simple obesity and 106 normal weight children were selected in the Affiliated Hospital of North China Coal Medical College from June 2004 to September 2007.5 mL fast blood sample was taken from each patient. Plasma fibrinogen level and molecular reactivity were measured with Assist Plasm Fibrinogen Activity Assay System. Polymerize chain reaction and restriction enzyme digestion were used to detect the fibrinogen B??-148C/T gene polymorphism genes genotype. Results??The plasma fibrinogen level and the molecular activity of the children with simple obesity were significantly higher than that of the health control group ??P < 0.05??. The allele frequency in the children with simple obesity was higher than that of the health control group??P < 0.05??. The plasma fibrinogen level and the fibrin monomer polymerize velocity of theB??-148 TT+CT was higher than that of the CC type in the children with simple obesity. Conclusion??The simple obesity in children is associated with the high level and the strengthen molecular activity of fibrinogen. The fibrinogen B??-148C/T gene polymorphism may be an accumulative efficiency gene of children with simple obesity by influencing the level and the fibrinogen metamer polymerization velocity.