智能化的语言云预检分诊系统在急诊分诊中的应用

目的：探讨系统化预检分诊模式对急诊分诊质量的影响。方法：选取2017年1月至2018年6月期间我院急诊就诊的7282例患者作为研究对象,将其分为对照组(四级分诊模式,2017年1月至2017年9月,3641例)和观察组(系统化预检分诊模式,2017年10月至2018年6月,3641例),观察和比较两组分诊准确率,抢救成功率、死亡率、候诊意外发生率,以及患者满意度。结果：与对照组相比,观察组分诊准确率明显增高(P<0.05);与对照组相比,观察组抢救成功率显著提高,死亡率及候诊意外发生率均明显降低(P<0.05);与对照组相比,观察组患者满意度大幅改善(P<0.05)。结论：系统化预检分诊模式应用于急诊分诊过程后,分诊护士借助急诊预检快速分诊评估单,能够较为准确地评估患者病情并正确分级分诊,缩短预检分诊护士的分诊时间,提高满意度。     

The rare red cell phenotype Lan negative in Japanese

Two patients and a healthy blood donor were found to be Lan negative and to have anti-Lan in their sera. These are the first three examples of the Lan-negative phenotype to be described in Japanese. The families of two of the propositi were studied: the parents of both were first cousins and one of the propositi had a Lan-negative sibling. No Lan- negative examples were found in 15,000 Japanese donors screened with anti-Lan.     

Variation in induction of chromosomal beta-lactamase expression in strains of Enterobacter cloacae

Eight strains of Enterobacter cloacae with varying patterns of susceptibility to beta-lactam antibiotics were studied to compare the inducibility and expression of their beta-lactamase genes. These strains included two isolates from one patient, which differed in their susceptibility to cefamandole. A resistant strain constitutively produced large amounts of beta-lactamase; a sensitive strain produced an inducible beta-lactamase. Study of induction and growth characteristics of all strains revealed that the induction and the expression of inducible beta-lactamase genes may vary considerably among strains of E. cloacae.     

Variation in Blackbutt

The pattern of variation in a number of blackbutt (E. pilularis Sm.) stands is described.

Field observations suggested that this variation may have resulted from introgression with each of a number of inbreeding (renantherous) species, but this was not supported by progeny studies.

Some evidence is presented that inbreeding in blackbutt may be one of the factors helping maintain species stability.

Possible silvicultural significance of the variation pattern is examined.     

Variation in cytosolic protein expression between human colon tumors that differ with regard to differentiation class

Using a combination of two-dimensional gel electrophoresis, silver staining, and a 16-quadrant grid system, we established a set of composite patterns for the colon tumor cytosol proteins of well, moderately, and poorly differentiated tumors. These composite patterns were found to be characteristic of the three individual differentiation classes for colon tumors. The apparent relative molecular masses (Mr) of the resolved proteins ranged from 14,000 to 105,000 and their isoelectric points from pH 5.2 to 8.4. Although the vast majority of the proteins identified in the composite patterns were common, a comparison based upon these patterns revealed two qualitative and seven quantitative protein differences. The qualitative differences were identified by apparent Mr X 10(-3)/pI coordinates of 73/7.2 and 66/6.2. Quantitative differences were identified by Mr X 10(-3)/pI coordinates of 71/6.0, 59/6.7, 57/6.7, 56/5.4, 52/6.1, 30/5.8, and 18/6.2. These cytosolic differentiation marker proteins may facilitate the diagnosis, staging, and monitoring of human colon cancer.     

Variation of gene expression profile linked to p27 Ser phosphorylation status in MCF-7 cell line

Ser¹⁰ was the major phosphorylation site in p27^Kip1 (here after referred to as p27), accounting for 70% of the total phosphorylation of this protein, due to cytoplasmic shuttling. To further elucidate the mechanism for Ser¹⁰-mediated dysfunction of p27 during breast cancer progression. Affymetrix Human Genome U133A Array was used to identify differentially regulated genes between MCF-7 breast cancer cell lines stably-transfected with wild type and Ser¹⁰ mutant (substitution of Ser¹⁰ with Ala, S10A), alternatively. Then to confirm by RT-PCR then western blot partly. Microarray analysis showing that S10A, then abrogation of Ser¹⁰ phosphorylation, result in important changes in a large number of genes involved in the control of cell cycle, cell differentiation, metabolism, immune response and signal transduction. S10A induced cell cycle G₀ phase arrest by FACS method. And cell growth inhibition and abrogation of cytoplasmic translation. Our data indicate that abrogation of phosphorylation of Ser¹⁰ resulted in significant changes in gene expression profiles which mediate cell cycle redistribution, sub-cellular localization.     

Variation of adhesion molecule expression on human umbilical vein endothelial cells upon multiple cytokine application

Background: The crude drug “kaki-yô” is a traditional medicine used in Japan as a hypotensive drug. Methods: The effect of five triterpenoid compounds, isolated from leaves of Diospyros kaki on stimulus-induced superoxide generation and phosphorylation of tyrosine residues of protein in human neutrophils was investigated. The five compounds examined were -amyrin (A), uvaol (UV), ursolic acid (UA), 19 -hydroxy ursolic acid (HU) and 19 ,24-dihydroxy ursolic acid (DHU). Results: When the cells were preincubated with these compounds, the superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was significantly suppressed in a concentration-dependent manner. These compounds also suppressed the superoxide generation induced by arachidonic acid (AA) in high concentrations. In the case of the superoxide generation induced by phorbol 12-myristate 13-acetate (PMA), UA, HU and DHU suppressed the superoxide generation but A and UV gave no effect. When the cells were incubated with fMLP in UA, HU and DHU, fMLP-induced tyrosyl phosphorylation of 45 kDa proteins of the cells was dose-dependently suppressed in parallel to the suppression of fMLP-induced superoxide generation. Conclusions: Triterpenoid compounds suppress stimulus-induced superoxide generation and tyrosyl phosphorylation and may have pharmaceutical applications.     

高温诱导大肠癌细胞程序化死亡与p53基因转录表达改变的实验研究

目的：探讨Ｐ５３基因在高温诱导大肠癌细胞程序优化死亡过程中的转录表达变化,为高温治疗肿瘤提供分子生物学线索。方法选择几种常用高温治疗温度诱导大肠癌细胞呈现ＰＣＤ特征,应用免疫细胞化学和细胞原位杂交技术对含有不同类型Ｐ５３基因的ＨＴ－２９、Ｌｏｖｏ两大肠癌细胞Ｐ５３蛋白表达和Ｐ５３ｍＲＮＡ转录进行了对比分析。结果经４３℃处理３０ｍｉｎ后,两种细胞ｐ５３ｍＲＮＡ转录均有所增强。ＨＴ－２９细胞ｐ５３蛋白     

腹腔镜手术前脐孔准备方法的比较研究

目的 探讨腹腔镜手术脐孔术前准备的合适方法.方法 对施行腹腔镜手术的90例患者分别采取3种皮肤清洁准备方法,观察脐孔局部反应和分析微生物采样报告.结果 石油醚能有效清除脐孔污垢,对皮肤刺激性小;新洁尔灭酊具有较好的消毒效果但对皮肤有刺激性;百多邦对皮肤刺激性小但消毒效果低于新洁尔灭酊.结论 临床上应根据具体情况分析,采用合适的方法,从而达到腹腔镜手术脐孔术野的备皮要求.     

Variation in indirect cell-mediated lympholysis

The ability of an allograft recipient to respond to donor mononuclear cells in an indirect cell-mediated lympholysis (ICML) assay is an in vitro correlate of allograft rejection, but the value of this correlation depends upon the assay's reliability. We had observed inconsistency in the cytotoxic response of normal human mononuclear cells (MNC) to the same allogeneic stimulator MNC when cytotoxicity was measured repeatedly on different occasions by micro-ICML. We, therefore, investigated the extent and reasons for this inconsistency. Method variation, determined by duplicate ICML of 18 stimulator: responder MNC, was not statistically significant. Variation in cytotoxicity over time was greater but still not statistically significant. The contribution to method variation of 51Cr release from 3 different sets of target cells, cultured and labeled in duplicate, was minimal (6.33%). We then asked if in vitro generation of effector MNC under laboratory conditions was a major cause of ICML variation. We tested this using a stable transplant's in vivo sensitized effector cells against donor MNC in a direct CML (DCML) and obtained consistent results. Finally, to gain an understanding of some of the factors which might influence the generation of in vitro cytotoxicity, we measured the frequencies of cell surface antigens (DR, TAC, transferrin, Leu 2 and 3) concomitantly with ICML on day 6 of culture. Statistical analysis of the results led us to conclude that the micro-ICML is reproducible. The magnitude of lysis depends upon activated target cells (TAC- and transferrin-positive) and an increase in the proportion of helper/inducer to cytotoxic/suppressor T-lymphocytes during effector cell generation.     

Genetic Variation in Healthy Oldest-Old

Individuals who live to 85 and beyond without developing major age-related diseases may achieve this, in part, by lacking disease susceptibility factors, or by possessing resistance factors that enhance their ability to avoid disease and prolong lifespan. Healthy aging is a complex phenotype likely to be affected by both genetic and environmental factors. We sequenced 24 candidate healthy aging genes in DNA samples from 47 healthy individuals aged eighty-five years or older (the ‘oldest-old’), to characterize genetic variation that is present in this exceptional group. These healthy seniors were never diagnosed with cancer, cardiovascular disease, pulmonary disease, diabetes, or Alzheimer disease. We re-sequenced all exons, intron-exon boundaries and selected conserved non-coding sequences of candidate genes involved in aging-related processes, including dietary restriction (PPARG, PPARGC1A, SIRT1, SIRT3, UCP2, UCP3), metabolism (IGF1R, APOB, SCD), autophagy (BECN1, FRAP1), stem cell activation (NOTCH1, DLL1), tumor suppression (TP53, CDKN2A, ING1), DNA methylation (TRDMT1, DNMT3A, DNMT3B) Progeria syndromes (LMNA, ZMPSTE24, KL) and stress response (CRYAB, HSPB2). We detected 935 variants, including 848 single nucleotide polymorphisms (SNPs) and 87 insertion or deletions; 41% (385) were not recorded in dbSNP. This study is the first to present a comprehensive analysis of genetic variation in aging-related candidate genes in healthy oldest-old. These variants and especially our novel polymorphisms are valuable resources to test for genetic association in models of disease susceptibility or resistance. In addition, we propose an innovative tagSNP selection strategy that combines variants identified through gene re-sequencing- and HapMap-derived SNPs.     

Variation in intravaginal pressure measurements

The wide variation in intravaginal pressure measurements of the circumvaginal muscles (CVM) was studied in five subjects under well-controlled conditions. Previous research and clinical observations have indicated that fluctuations in the measurement of intravaginal pressure may be associated with time of day, day of testing, and existing stress factors. Subjects were assessed four times per day, on four consecutive days, for a total of 16 assessments. At each of the 16 conditions for a given subject, 10 CVM contractions lasting 12 seconds each were recorded and the variables, maximum pressure (MP10), peak maximum pressure (PMP), and abdominal pressure were analyzed. The within-subject variance was 15.5 (SD = 3.9); the between-subject variance was 132.4 (SD = 11.5). The effects of day, time, and stressor were analyzed by ANOVA specifically designed for variance estimates; no significant differences were found. The clinical observations that led to the study were not supported when systematically investigated. However, consistent data collection procedures appeared to reduce within-subject variance.     

Contingent Negative Variation in Migraine

The Contingent Negative Variation (CNV) is an event-related slow potential. It was recorded in healthy volunteers (n = 8) and in patients suffering from migraine without (n = 12) or with (n = 5) aura, during one (CNV1) and three second (CNV3) foreperiods in a forewarned reaction time task. CNV1 was recorded at the vertex while CNV3 was recorded at multiple electrode sites to assess topographical differences. Seven out of twelve migraine patients without aura had increased CNV1 amplitudes. CNV3 amplitudes were increased as well, but only at electrode positions C3 and C4 and not at Fz. CNV3, which allows for analysis of both an early and a late CNV component, could improve the discrimination of migraine without aura beyond that of CNV1. In migraine with aura all CNV parameters were at control levels, confirming previous results. The data obtained are discussed in terms of arousal, activation and stress and the "biobehavioral model of migraine" (Welch, 1986).     

Variation of MDR proteins expression and activity levels according to clinical status and evolution of CML patients

The involvement of the multidrug resistance (MDR) mediated by ABC transporter proteins P-glycoprotein (Pgp) and multidrug resistance-associated protein-1 (MRP1) overexpressions in patients with chronic myeloid leukemia (CML) are not completely understood. Pgp and MRP1 expressions and activity were analyzed in samples from 158 patients with chronic myeloid leukemia (CML). Using flow cytometry, Pgp expression was more frequently observed in early chronic (P = 0.00) and in advanced (P = 0.02) CML phases when it was compared to MRP1 expression. Variation of MDR expression and activity were observed during the CML evolution in patients previously treated with interferon and imatinib. In the K562-Lucena cell line, Pgp positive, imatinib caused an enhancing in Pgp expression at protein and mRNA levels, whereas in the Pgp negative cell line, this drug was capable of decreasing MDR1/Pgp mRNA levels. Our result emphasizes the importance of understanding the different aspects of MDR status in patients with CML when they are under investigation in determining imatinib resistance.     

Circadian Variation in Circulating Platelet Aggregates

The mitochondrial F₁F_o adenosine triphosphate (ATP) synthase is one of the most thoroughly studied enzyme complexes known. Yet, a number of new observations suggesting that the enzyme is also located on the cell surface necessitate further investigation. While the mitochondrial synthase utilizes the proton gradient generated by oxidative phosphorylation to power ATP synthesis, the cell surface synthase has instead been implicated in numerous activities, including the mediation of intracellular pH, cellular response to antiangiogenic agents, and cholesterol homeostasis. Intriguingly, a common thread uniting these various models of cell surface ATP synthase functions is the apparently caveolar distribution of the enzyme. Recent studies concerning the cell surface ATP synthase manifest applications in the regulation of serum cholesterol levels, cellular proliferation and antitumor strategies. This review addresses the expression, interactions, functions, and consequences of inhibition of cell surface ATP synthase, an enzyme now displaying a shift in paradigm, as well as of location.