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1.
Dragonas C Wagner JT Heppner HJ Bertsch T Muhlberg W Wicklein S Pahl A Diewald C Bachmakov I Sieber CC Fromm MF 《European journal of clinical pharmacology》2008,64(4):367-372
Objective Digitoxin is a known substrate of the efflux pump P-glycoprotein (gene name: ABCB1). P-glycoprotein expression was shown to be modulated by single nucleotide polymorphisms in the ABCB1 gene, but it remains unclear whether these polymorphisms influence digitoxin blood levels. Our objective was to examine the
association of ABCB1 C3435T genotype and elevated serum digitoxin concentrations (SDC) in a cohort of 77 geriatric patients consecutively admitted
to a geriatric department over a 12-month period.
Methods The impact of ABCB1 3435 CC, CT, and TT genotypes on SDC and SDC normalized for daily digitoxin dosage and body weight was assessed by multivariate
regression analysis.
Results Among participants, 18 (23%) had the CC, 36 (47%) the CT, and 23 (30%) the TT genotype. Adjusting for relevant covariates,
no significant association of ABCB1 C3435T genotype and SDC or normalized SDC was detected. Mean SDC was 22.4 ng/ml (95% CI 18.9–25.9) for the TT, 21.8 ng/ml
(95% CI 18.1–25.5) for the CT, and 25.7 ng/ml (95% CI 20.6–30.8) for the CC genotype. The means for normalized SDC were 5.2
kg·l−1 (95% CI 4.3–6.1) for the TT, 6.1 kg·l−1 (95% CI 4.7–7.5) for the CT, and 6.2 kg·l−1 (95% CI 4.6–7.7) for the CC genotype.
Conclusion In this sample of frail geriatric patients, the impact of ABCB1 C3435T genotype on serum digitoxin concentration was not of major relevance. Regular monitoring of digitoxin blood levels
and surveillance of appropriate drug use remain the best ways to prevent digitoxin intoxications in the elderly. 相似文献
2.
Freitas AR Novais C Correia R Monteiro M Coque TM Peixe L 《International journal of antimicrobial agents》2011,38(2):174-176
In this study, the in vitro activity of tigecycline against 1140 enterococci collected from humans, food products, animals and the environment in Portugal (1996-2008) was analysed. Ten isolates (seven Enterococcus faecalis and three Enterococcus spp.) non-susceptible to tigecycline (minimum inhibitory concentrations of 0.5-1.0 mg/L), which were also resistant to tetracycline and minocycline, were mostly observed in samples collected before the introduction of tigecycline in the therapeutic arsenal. The E. faecalis isolates were recovered from hospitalised patients (n = 2; ST319/CC2 and ST34), healthy humans (n = 2; ST21/CC21), chicken meat (n = 1; ST260) as well as from two swine samples. The remaining isolates were also recovered from chicken meat (n = 1; Enterococcus gallinarum) and swine (n = 2; Enterococcus hirae and Enterococcus spp.). Recovery of enterococcal isolates with reduced susceptibility to tigecycline amongst different reservoirs, including animals for food consumption, suggests that selection of tigecycline-resistant isolates by antibiotics other than tigecycline might occur in non-clinical settings. 相似文献
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Talwar GP Dar SA Rai MK Reddy KV Mitra D Kulkarni SV Doncel GF Buck CB Schiller JT Muralidhar S Bala M Agrawal SS Bansal K Verma JK 《International journal of antimicrobial agents》2008,32(2):180-185
A polyherbal cream (Basant) has been formulated using diferuloylmethane (curcumin), purified extracts of Emblica officinalis (Amla), purified saponins from Sapindus mukorossi, Aloe vera and rose water along with pharmacopoeially approved excipients and preservatives. Basant inhibits the growth of WHO strains and clinical isolates of Neisseria gonorrhoeae, including those resistant to penicillin, tetracycline, nalidixic acid and ciprofloxacin. It has pronounced inhibitory action against Candida glabrata, Candida albicans and Candida tropicalis isolated from women with vulvovaginal candidiasis, including three isolates resistant to azole drugs and amphotericin B. Basant displayed a high virucidal action against human immunodeficiency virus HIV-1NL4.3 in CEM-GFP reporter T and P4 (Hela-CD4-LTR-betaGal) cell lines with a 50% effective concentration (EC50) of 1:20000 dilution and nearly complete (98-99%) inhibition at 1:1000 dilution. It also prevented the entry of HIV-1(IIIB) virus into P4-CCR5 cells (EC50 approximately 1:2492). Two ingredients, Aloe and Amla, inhibited the transduction of human papillomavirus type 16 (HPV-16) pseudovirus in HeLa cells at concentrations far below those that are cytotoxic and those used in the formulation. Basant was found to be totally safe according to pre-clinical toxicology carried out on rabbit vagina after application for 7 consecutive days or twice daily for 3 weeks. Basant has the potential of regressing vulvovaginal candidiasis and preventing N. gonorrhoeae, HIV and HPV infections. 相似文献
5.
目的 探讨注射用头孢哌酮舒巴坦钠联合注射用替加环素治疗多重耐药肺炎克雷伯菌肺炎的应用效果。方法 选取2020年1月—2022年12月铜陵市人民医院收治的76例多重耐药肺炎克雷伯菌肺炎患者作为研究对象,按随机数字表法将所有患者分为对照组和治疗组,各38例。对照组静脉滴注注射用替加环素,50 mg/次,1次/12 h。治疗组在对照组治疗基础上静脉滴注注射用头孢哌酮钠舒巴坦钠,2 g/次,生理盐水100 mL稀释,1次/6 h。两组均连续治疗7 d。比较两组的临床疗效、细菌清除情况和血清炎症指标。结果 治疗后,治疗组的临床治愈率为78.95%,明显高于对照组的临床治愈率55.26%(P<0.05)。治疗后,治疗组的细菌清除率为81.58%,明显高于对照组的细菌清除率55.26%(P<0.05)。治疗后,两组血清指标C-反应蛋白(CRP)、白细胞介素-6(IL-6)和降钙素原(PCT)水平均降低(P<0.05),且治疗组血清炎症指标低于对照组(P<0.05)。结论 注射用头孢哌酮舒巴坦钠联合注射用替加环素治疗多重耐药肺炎克雷伯菌肺炎的效果显著,有效提高细菌清除率,减轻机体炎症反应。 相似文献
6.
Yu Zhang Jia-li Li Qian Fu Xue-ding Wang Long-shan Liu Chang-xi Wang Wen Xie Zhuo-jia Chen Wen-ying Shu Min Huang 《Acta pharmacologica Sinica》2013,34(4):555-560
Aim:
Cyclosporine requires close therapeutic drug monitoring because of its narrow therapeutic index and marked inter-individual pharmacokinetic variation. In this study, we investigated the associations of CYP3A4, CYP3A5, ABCB1, NFKB1, and NR1I2 polymorphisms with cyclosporine concentrations in Chinese renal transplant recipients in the early period after renal transplantation.Methods:
A total of 101 renal transplant recipients receiving cyclosporine were genotyped for CYP3A4*1G, CYP3A5*3, ABCB1 C1236T, G2677T/A, C3435T, NFKB1 −94 ins/del ATTG, and NR1I2 polymorphisms. Cyclosporine whole blood levels were measured by a fluorescence polarization immunoassay. Trough concentrations of cyclosporine were determined for days 7-18 following transplantation.Results:
The dose-adjusted trough concentration (C0) of cyclosporine in ABCB1 2677 TT carriers was significantly higher than that in GG carriers together with GT carriers [90.4±24.5 vs 67.8±26.8 (ng/mL)/(mg/kg), P=0.001]. ABCB1 3435 TT carriers had a significantly higher dose-adjusted C0 of cyclosporine than CC carriers together with CT carriers [92.0±24.0 vs 68.4±26.5 (ng/mL)/(mg/kg), P=0.002]. Carriers of the ABCB1 1236TT-2677TT-3435TT haplotype had a considerably higher CsA C0/D than carriers of other genotypes [97.2±21.8 vs 68.7±26.9 (ng/mL)/(mg/kg), P=0.001]. Among non-carriers of the ABCB1 2677 TT and 3435 TT genotypes, patients with the NFKB1 −94 ATTG ins/ins genotype had a significantly higher dose-adjusted C0 than those with the −94 ATTG del/del genotype [75.9±32.9 vs 55.1±15.1 (ng/mL)/(mg/kg), P=0.026].Conclusion:
These results illustrate that the ABCB1 and NFKB1 genotypes are closely correlated with cyclosporine trough concentrations, suggesting that these SNPs are useful for determining the appropriate dose of cyclosporine. 相似文献7.
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Otilia Antal Mariann Karisztl-Gcsi Anna Farkas Attila Kovcs Andrs cs Norbert Tr Gyula Kiss Martin L. Saker Jnos Gyri Gspr Bnfalvi gnes Vehovszky 《Toxicon》2011,57(6):831-840
Cylindrospermopsis raciborskii is becoming a major concern among cyanobacteria, due to its potential ability to produce toxic metabolites. We assessed the cytotoxic potential of four C. raciborskii strains (ACT 9502, ACT 9503, ACT 9504 and ACT 9505) isolated from Lake Balaton (Hungary), by lactate dehydrogenase (LDH) leakage measurements and by detecting morphological alterations in CHO-K1 (Chinese Hamster Ovary) cells. The Australian AQS (cylindrospermopsin producer) strain of C. raciborskii and purified cylindrospermopsin (CYN) were used as positive references in both the biochemical and morphological studies. Chemical analysis for known cyanotoxins was performed on aqueous extracts of ACT and AQS strains by the HPLC-MS technique.Comparing threshold values of LDH leakage data, different toxic potentials of cyanobacterial extracts are suggested in short term (3 h) and long (24 h) exposure regimes. In the acute (3 h) experiments the aqueous extract of the ACT 9505 strain proved to be most toxic (EC50 = 7.4 mg mL−1), while after 24 h the ACT 9504 extract was the most effective (EC50 = 0.65 mg mL−1). The extract of the AQS strain and the purified CYN exerted most of their toxic effects after 3 h exposure (EC50 = 0.74 mg mL−1, and 0.9 μg mL−1 respectively).The morphological changes of CHO-K1 cells induced by the crude extracts of the ACT strains included fragmentation of the actin filaments then relocation of the depolymerized actin to the perinuclear region, resulting cell rounding and loss of adhesion. Exposure of CHO-K1 cells to the crude extract of the AQS strain, moreover, resulted cell shrinking and formation of filopodia, i.e. distinctly different cytological alterations from that induced by the ACT extracts and the purified CYN.Chemical analysis of the cyanobacterial crude extracts confirmed the presence of cylindrospermopsin in the extract of the AQS strain (8.5 mg CYN g−1 dry weight), and none of the presently known cyanotoxins have been analytically confirmed in the extracts of the ACT strains isolated from the Lake Balaton.Although a significant toxicity of all four ACT C. raciborskii strains is confirmed by both biochemical and morphological studies, our results also pointed out the necessity of further studies to identify the toxic, but still unknown metabolic components produced by these cyanobacterial members of the phytoplankton communities. 相似文献
9.
目的对不同来源的湖南产莲子中黄曲霉毒素G_1、G_2、B_2、B_1进行高效液相色谱-光化学衍生法测定。方法采用高效液相色谱–光化学衍生法,采用岛津GL Inertsil ODS-3色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇–乙腈–水(35∶13∶52),柱温35℃;光化学衍生器(254 nm)激发波长λ_(ex)=360 nm,发射波长λ_(ex)=450 nm。结果黄曲霉毒素G_1、G_2、B_2、B_1分别在6.7~33.3、11.4~56.8、10.3~51.5、4.1~20.3 pg线性关系良好;平均回收率分别为98.07%、97.72%、96.11%、99.52%,RSD值分别为1.69%、1.40%、2.72%、1.34%(n=6)。9批莲子样品中,有6批未检出黄曲霉毒素,来自于农贸市场农户自存的2批次检出黄曲霉毒素B_1,实验室塑料袋包装储存1年的1批检出黄曲霉毒素B_1、B_2,但质量分数均低于法定标准限量。结论不同来源湖南产莲子中黄曲霉毒素质量分数均低于《中国药典》2020年版规定限量。该法测定结果准确、重复性良好,可为完善莲子安全性控制和质量标准提升提供实验依据。 相似文献
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目的建立高效液相色谱–光化学衍生–荧光检测法测定沉香药材中黄曲霉毒素B1、B2、G1、G2。方法采用高效液相色谱法,通过免疫亲和柱提取和净化,荧光检测器检测。Agilent Zorbax Ecilpse Plus C18色谱柱(250 mm×4.6 mm,5μm);流动相:甲醇–水(45∶55);体积流量:0.8 m L/min;柱温:30℃;进样盘温度:4℃;荧光激发波长为360 nm,发射波长为450 nm。结果黄曲霉毒素B1、B2、G1、G2分别在9.3~74.4、3.0~24.0、9.3~74.4、3.5~28.0 pg线性关系良好,r均大于0.998 0;检测限分别为1.86、0.60、1.86、0.70 pg,定量限分别为7.44、2.40、7.44、2.80 pg。平均回收率分别为78%、92%、82%、99%,RSD值分别为4.4%、3.0%、4.3%、2.8%。结论所建立的方法结果准确、重复性、稳定性均良好,可用于沉香药材中黄曲霉毒素的质量控制。 相似文献
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目的 研究丹参、红花药对配伍前后对大鼠肝药酶亚型CYP1A2、CYP2E1和CYP3A4活性的影响。方法 分别选用咖啡因、氯唑沙宗和咪达唑仑作为CYP1A2、CYP2E1和CYP3A4的探针药物。将大鼠随机分为4组,即空白对照组、丹参(1.2 g生药/kg)组、红花(0.4 g生药/kg)组、丹参(1.2 g生药/kg)+红花(0.4 g生药/kg)组,按上述剂量ig给药7 d。于末次给药后30 min,尾iv探针药物咖啡因、氯唑沙宗和咪达唑仑溶液,在不同的时间点取血进行检测;以甲硝唑为内标,采用HPLC法检测探针药物咖啡因、氯唑沙宗和咪达唑仑的量,评价各药物组对大鼠CYP3A4、CYP2E1和CYP1A2活性的影响。结果 与空白对照组比较,丹参组咖啡因、氯唑沙宗和咪达唑仑的清除率(CL)有所增强,曲线下面积(AUC)减少,其半衰期(t1/2)有减少趋势,但差异均不显著;红花组咖啡因和氯唑沙宗的CL有所降低,但差异不显著,咪达唑仑的CL显著降低(P<0.01),氯唑沙宗的AUC增加,但差异不显著,咖啡因和咪达唑仑的AUC明显增加(P<0.05、0.01);丹参+红花组咖啡因和氯唑沙宗的CL明显降低(P<0.05),曲线下面积(AUC)明显增加(P<0.05),其t1/2有延长趋势,但差异不显著。结论 丹参、红花配伍后对CYP450亚型CYP1A2和CYP2E1有抑制作用,这可能是丹参、红花配伍协同增效的作用机制之一。 相似文献
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目的 研究艾叶制炭前后对人主动脉内皮细胞组织因子途径抑制物(TFPI)、内皮细胞血管性假血友病因子(vWF)、NO含量的影响,以及对大鼠血小板氨基末端激酶-1(JNK1)表达水平的影响,阐明艾叶炭止血机制。方法 将体外培养的人主动脉内皮细胞(HEAC)随机分组,分别给予不同极性(石油醚、二氯甲烷、醋酸乙酯、正丁醇、水、水提物、鞣质、总黄酮)部位的艾叶生、炭品,终质量浓度为200、100、50 μg/mL,培养48 h后,取细胞培养液试剂盒法测定TFPI、vWF、NO含量;采用免疫蛋白印迹方法检测艾叶制炭前后各部位对大鼠血小板JNK1磷酸化水平的影响。结果 与对照组及相应生品组比较,艾叶炭品二氯甲烷和鞣质部位TFPI、NO含量显著降低,vWF含量显著升高(P<0.01);大鼠血小板JNK1磷酸化水平显著升高(P<0.01)。与对照组比较,艾叶鞣质部位TFPI、NO含量显著降低,vWF含量显著升高(P<0.01);大鼠血小板JNK1磷酸化水平显著升高(P<0.01)。结论 二氯甲烷和鞣质部位是艾叶炭的主要止血作用部位,其止血机制为降低TFPI、NO含量、增加vWF的含量;明显提高血小板JNK1的磷酸化水平。 相似文献
14.
UGT1A1基因是参与人体代谢循环的重要基因,随着药物基因组学的发展,发现其基因多态性与某些药物代谢水平相关,进而影响疾病的发生、发展及治疗等诸多方面。随着研究进展,UGT1A1的底物在不断扩展,包括胆红素、雌激素、伊立替康及其他一些药物已有研究。研究UGT1A1基因多态性对药物代谢情况的影响,在临床疾病的诊治、预后判断及药物不良反应等方面有重要的指导意义。 相似文献
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Oxidative damage to DNA induced by benzene is an important mechanism of its genotoxicity, which leads to chronic benzene poisoning (CBP). Therefore, genetic variation in DNA repair genes may contribute to susceptibility to CBP in the exposed population. We hypothesized that single nucleotide polymorphisms (SNPs) in hMTH1, hOGG1 and hMYH genes are associated with risk of CBP. We genotyped SNPs at codon 83 of hMTH1, codon 326 of hOGG1, and codon 324 of hMYH in 152 CBP patients and 152 healthy workers occupationally exposed to benzene without poisoning manifestations. The genotypes were determined by polymerase chain reaction-restrained fragment length polymorphism (PCR-RFLP) technique. There were 2.51-fold [adjusted odds ratio (ORadj), 2.51; 95% CI, 1.14-5.49; P = 0.02] and 2.49-fold (ORadj, 2.49; 95% CI: 1.52-4.07; P < 0.01) increased risk of CBP for individuals carrying genotypes of hMTH1 83Val/Met + Met/Met and hOGG1 326Cys/Cys, respectively. Compared with individuals carrying genotypes of hOGG1 326Cys/Cys and hMYH 324His/His at the same time, there was a 0.33-fold (ORadj, 0.33; 95% CI: 0.15-0.72; P < 0.05) decreased risk of CBP for those with genotypes of hOGG1 326Ser/Cys + Ser/Ser and hMYH 324His/Gln + Gln/Gln. In the smoking group, there was a 0.15-fold (ORadj, 0.15; 95% CI, 0.03-0.68; P = 0.01) decreased risk of CBP for subjects carrying genotypes of hMYH 324His/Gln + Gln/Gln compared with those of genotype of hMYH 324His/His. Therefore, our results suggested that polymorphisms at codons 83 of hMTH1 and codon 326 of hOGG1 might contribute to CBP in a Chinese occupational population. 相似文献
16.
目的探讨替加环素治疗复杂性腹腔感染的效果及安全性。方法选择2018年7月至2020年8月某三甲医院收治的76例复杂性腹腔感染患者为研究对象,采用随机数字表法分为观察组(36例)及对照组(40例),观察组采用替加环素治疗,对照组采用亚胺培南西司他丁钠治疗。测定治疗前后白细胞计数、中性粒细胞计数、降钙素原(PCT)、C反应蛋白(CRP)水平,测定T淋巴细胞亚群比例,评价疗效及安全性。结果 76例患者共分离出98例细菌,以鲍曼不动杆菌、肺炎克雷伯菌、大肠埃希菌等为主。观察组及对照组总体有效率分别为72.22%、72.50%,组间比较差异无统计学意义(P>0.05)。两组治疗后CRP、PCT、白细胞计数、中性粒细胞计数降低(P<0.05),CD3+ T淋巴细胞亚群比例、CD4+ T淋巴细胞亚群比例及CD4+/CD8+值显著升高(P<0.05),两组组间比较差异无统计学意义(P>0.05)。观察组和对照组总体不良反应发生率分别为13.89%、15.00%,组间比较差异无统计学意义(P&... 相似文献
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Ruiz-Martínez L López-Jiménez L Fusté E Vinuesa T Martínez JP Viñas M 《International journal of antimicrobial agents》2011,38(5):398-402
The aims of this study were to ascertain the presence and spread of class 1 integrons amongst environmental and clinical isolates of Pseudomonas aeruginosa and to characterise their variable regions. A total of 76 isolates (56 clinical and 20 environmental) were studied. The presence of plasmids was explored, and polymerase chain reaction (PCR) was used for integron detection. All amplicons were sequenced. PCR detected class 1 integrons in 26 of the 56 clinical isolates; environmental isolates were integron-free. No plasmids were found, thus all the integrons found are possibly on the chromosome. Most isolates presented one amplicon, except PA110514 and PA116136, which showed two PCR products each. Variable regions revealed that 18 strains carried only one gene involved in aminoglycoside resistance, whereas in 3 strains gene cassettes were not found. The most prevalent cassettes amongst isolates were those encoding aminoglycoside adenyltransferase B (aadB). Several of the strains had acquired the same or a highly similar cassette array as those detected in geographically distant P. aeruginosa. This finding suggests that contact with bacterial reservoirs contributes to the evolution of this pathogen towards multiresistance. Empty structures found may represent a reservoir increasing the capacity to adapt to the environment. However, these integrons are not retained when the selective pressure disappears. It is hypothesised that integrons containing gene cassettes are crucial vehicles for the rapid horizontal transfer of resistance. If this is so, reduced use of antibiotics may lead to a significant decrease in the carriage of integrons amongst P. aeruginosa strains. 相似文献
18.
目的 探讨VKORC1-3673G>A、CYP2C9*3、CYP4F2 rs2108622和CYP2C19*2位点基因多态性对中国汉族房颤患者华法林维持剂量的影响。方法 收集107例服用华法林达维持剂量的汉族房颤患者的血样和临床相关资料,应用PCR-RFLP法检测VKORC1-3673G>A、CYP2C9*3、CYP4F2 rs2108622和CYP2C19*2基因型,采用独立样本t检验分析基因型与华法林维持剂量的相关性。多元线性回归建立给药模型,探讨基因多态性对华法林维持剂量的影响。结果 VKORC1-3673G>A、CYP2C9*3、CYP4F2 rs2108622基因多态性和患者年龄、体质量能解释45.2%的华法林维持剂量差异。CYP2C19*2基因多态性对本研究人群华法林维持剂量无影响。结论 VKORC1-3673G>A、CYP2C9*3、CYP4F2 rs2108622基因多态性显著影响中国汉族房颤患者的华法林维持剂量。 相似文献
19.
目的 研究高脂血症患者SLCO1B1基因多态性与阿托伐他汀安全性及有效性的相关性。方法 收集金华市人民医院2017年4月—2018年4月在门诊确诊为高脂血症患者的基本资料,测定纳入患者的SLCO1B1 c.388A>G和c.521T>C的基因多态性,定期随访受试者,并定期测定其甘油三酯、胆固醇、低密度脂蛋白胆固醇及肌酸激酶等相关实验室检查指标。结果 纳入患者SLCO1B1 c.388A>G和c.521T>C等位基因频率分别为72.8%和15.9%。随访期结束后不同基因型患者的血清血脂指标变化率无明显差异。SLCO1B1 c.521T>C基因多态性与阿托伐他汀的安全性有相关性(P=0.005)。结论 SLCO1B1c.388A>G基因多态性对阿托伐他汀降脂疗效及安全性无影响。SLCO1B1 c.521T>C基因多态性与阿托伐他汀的降脂疗效无相关性,但对其安全性有一定影响。 相似文献
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New 2,6-piperidinediones 2a–g and 4a–d were prepared by initial condensation of aromatic aldehydes or cycloalkanones with cyanoacetamide to give α-cyanocinnamides
la–g or cycloalkylidenes 3a,b which underwent Michae1 addition with ethyl cyanoacetate or diethylmalonate. Compounds 4a–d were alkylated by various alkyl halides to produce the N-alkylated 2,6-piperidinedione derivatives 5a–m.
Some new selected compounds 2a–c,f, 4a–d & 5e,h,j were pharmacologically evaluated for potential anticonvulsant, sedative and analgesic activities. These compounds exhibited
significant anticonvulsant and analgesic effects after a single I.P. administration 100 mg/kg b.wt. . On the other hand all
the investigated compounds induced hypnotic activity and prolonged the phenobarbital sodium- induced sleep as compared with
the control group and the most potent compound was found to be 2f. 相似文献