人类免疫缺陷病毒感染者T淋巴细胞亚群增殖、活化与疾病进展的相关性

目的 探讨慢性未治疗HIV/AIDS患者T淋巴细胞增殖活化与疾病进展的相关性.方法 以16例健康人及49例慢性未治疗的HIV/AIDS患者为研究对象,HIV/AIDS患者根据CD4+T淋巴细胞计数分为CD4+T淋巴细胞<200×106/L组、(200～350)X106/L组、>350X106/L组.分离患者外周血单个核细胞(PBMC),Ki-67标记细胞增殖,CD38标记细胞活化,流式细胞仪检测各项指标.数据采用单因素方差分析.结果CD4+T淋巴细胞<200×106/L组Ki-67+CD4+T 淋巴细胞比例为7.92%±4.37%,高于健康对照组的0.39%±0.24%、(200～350)×106/L组的2.61%±2.12%和>350X 106/L组的2.65%±2.13%,差异均有统计学意义(F=21.961,P<0.01);CD4+T淋巴细胞<200X106/L组Ki-67+CD8+T淋巴细胞比例为2.87%±1.13%,高于健康对照组的0.15%±0.90%、(200～350)×106/L组的1.40%±1.17%和>350×106/L组的1.22%±0.80%,差异均有统计学意义(F=19.203,P<0.01).且Ki-67+CD4+和Ki-67+CD8+T淋巴细胞比例与CD4+T淋巴细胞计数呈负相关(r=-0.654,r=-0.539;均P<0.01),而与病毒载量无关.CD4+T淋巴细胞<200×106/L组CD38+CD4+、CD38+CD8+T淋巴细胞比例分别为44.14%±20.65%和50.64%±21.08%,健康对照组为10.22%±3.98%和6.46%±3.99%,(200～350)×106/L组为16.03%±10.20%和19.33%±13.43%,>350×106/L组为13.69%±10.70%和16.98%±15.75%(F=14.333,F=15.412;均P<0.01),且CD4+和CD8+T淋巴细胞Ki-67的表达程度与CD38呈正相关(r=0.527,r=0.391;均P=0.002).结论 随着慢性HIV/AIDs疾病的进展,T淋巴细胞的增殖与活化均显著增高,T淋巴细胞活化可能是免疫持续激活的结果.     

有效的高效抗反转录病毒治疗后低CD4/CD8比值HIV/AIDS患者免疫学特征分析

目的对比研究有效的高效抗反转录病毒治疗(highly active antiretroviral therapy,HAART)后低CD4/CD8比值组与高CD4/CD8比值组患者相关免疫学特征。方法选择接受HAART≥96周、CD4~+T淋巴细胞≥500/μl、HIV载量低于检测值下限的HIV感染者/AIDS患者(HIV/AIDS患者)109例,从中选出CD4/CD8比值≥0.82的患者28例及CD4/CD8比值≤0.48的患者28例,对比分析2组T淋巴细胞上CD38、人类白细胞抗原DR(HLA-DR)、CD27、CD28、CD45RA及CD31的表达,进而分析2组外周血T淋巴细胞的免疫活化与免疫老化状况。结果①低CD4/CD8比值组的基线CD4~+T淋巴细胞计数和基线CD4/CD8比值较高CD4/CD8比值组低(P0.05);②低CD4/CD8比值组HLA-DR~+CD8~+T淋巴细胞频率、处于中期分化阶段(CD27~-CD28~+)的CD4~+T淋巴细胞频率及处于晚期分化阶段(CD27~-CD28~-)的CD4~+和CD8~+T淋巴细胞频率高于高CD4/CD8比值组(P均0.05),而处于早期分化阶段(CD27~+CD28~+)的CD4~+和CD8~+T淋巴细胞频率低于高CD4/CD8比值组(P均0.05);③低CD4/CD8比值组纯真CD4~+和与CD8~+T淋巴细胞频率以及CD31~+纯真CD8~+T淋巴细胞的频率低于高CD4/CD8比值组(P均0.05)。结论①低基线CD4~+T淋巴细胞计数和低基线CD4/CD8比值HIV/AIDS患者有效HAART后CD4/CD8比值仍然相对较低,应及早起始HAART,预防CD4/CD8低比值持续的发生;②有效HAART后低CD4/CD8比值的HIV/AIDS患者表现出更为严重的免疫活化和免疫老化现象,针对这些免疫紊乱应给予除HAART外新的干预措施。     

HIV感染者/AIDS患者外周血CD38 HLA-DR分子在CD4+ CD8+T淋巴细胞上的表达

目的　观察国内艾滋病病毒 (HIV)感染者 /艾滋病 (AIDS)患者外周血CD38、HLA DR分子在CD+4 、CD+8T淋巴细胞上表达的变化 ,并探讨这些变化的临床意义。方法　用流式细胞仪检测 5 1例正常对照、14例HIV感染者和 3 6例AIDS患者的外周血CD+4 、CD+8T淋巴细胞表面的CD38、HLA DR分子的表达 ,用分枝DNA(bDNA)法检测 11例HIV感染者和 18例AIDS患者的血浆病毒载量。结果　CD+4 HLA DR+细胞百分比显示 ,AIDS组显著高于正常组及HIV组 ;CD+8HLA DR+T细胞百分比显示HIV组与AIDS组间无差异 ,而它们均显著高于正常组。CD+8、CD38+细胞百分比则是AIDS组 >HIV组 >正常组 ,CD+8CD38+、CD+8HLA DR+、CD+4 HLA DR+细胞百分比与病毒载量显著正相关。结论　在HIV感染过程中 ,HLA -DR+、CD38+在CD+4 、CD+8T淋巴细胞上的表达均显著增加 ,反映T淋巴细胞异常激活 ;尤其是CD+8CD38+细胞百分比随着疾病进展逐渐升高 ,预示疾病进展程度。在评价HIV感染者和AIDS患者的免疫状况时 ,不仅要考虑免疫细胞数量和功能的变化 ,还应考虑免疫细胞的激活水平     

HIV／AIDS患者血浆病毒载量及外周血T淋巴细胞亚群的变化

目的：观察国内HIV／AIDS患者血浆病毒载量和外周血CD4^ 、CD8^ T淋巴细胞的变化，探讨这些变化的临床意义。方法：选择未经抗病毒治疗的HIV／AIDS患者124例，用bDNA法检测血浆病毒载量，并用流式细胞仪检测外周血CD4^ 、CD8^ T淋巴细胞。结果：AIDS患者的血浆病毒载量明显高于HIV感染者，血浆病毒载量与CD4^ 细胞计数呈显著负相关，但其最高峰位于CD4^ 细胞计数100／μl处，然后随着CD4^ 细胞计数的下降而减少。CD4^ T细胞计数为AIDS组＜HIV组＜正常对照组：HIV感染者的CD8^ T细胞计数显著高于正常组和AIDS组，而AIDS患者CD8^ T细胞数则随着CD4^ T细胞减少而下降。结论：血浆病毒载量随着疾病进展而显著升高，但在疾病晚期则有所降低。外周血CD4^ T细胞计数随着疾病的进展而进行性减少；CD8^ T细胞计数在感染早期显著升高，进入晚期则减少。在评价HIV感染者和AIDS患者病情时，应结合病毒载量、CD4^ 、CD8^ T细胞计数综合分析。     

HIV感染者/AIDS患者与肿瘤病人T淋巴细胞亚群数量的比较

目的　探讨艾滋病病毒 (HIV)感染者和艾滋病 (AIDS)患者与肿瘤病人CD+4和CD+8T淋巴细胞 (TH 和TS 细胞 )数量的变化并进行比较。方法　用流式细胞仪检测 78例HIV感染者 /AIDS患者和 37例肿瘤病人外周血中TH 和TS 细胞的数量以及计算TH/TS 的比值 ,组间比较采用双侧t检验。结果　HIV感染者 /AIDS患者TH细胞显著减少 ,且下降的幅度大 ,TH<5 0 0 / μl的占 83 3% ,其中 <2 0 0 / μl的占 5 6 4 % ,TH/TS 平均为 0 2 9;肿瘤病人TH 细胞计数也明显降低 ,TH<5 0 0 / μl的占 70 3% ,其中 <2 0 0 / μl的占 18 9% ,TH/TS 平均为 1 2 6。同一水平CD+4T细胞计数所对应的CD+8T细胞数量 ,HIV感染者 /AIDS患者高于肿瘤病人 ;两组病人TS 数量都随TH 数量的升高而增加。HIV感染者 /AIDS患者TH 细胞计数 <肿瘤病人 ,TS 细胞计数 >肿瘤病人 ,HIV感染者 /AIDS患者TH/TS 比值倒置 ,而肿瘤病人TH/TS 比值 >1。结论　HIV感染者 /AIDS患者和肿瘤病人存在不同程度的细胞免疫功能损害 ,T淋巴细胞亚群的检测可作为两者免疫诊断和免疫功能监测的指标之一 ,对于病人的治疗和预后有一定的意义     

未接受HAART的HIV/AIDS患者血清隐球菌抗原阳性率调查

目的调查未接受高效抗反转录病毒治疗(highly active antiretroviral therapy,HAART)的HIV/AIDS患者血清隐球菌抗原的阳性率。方法回顾性调查我院感染科2012年1—9月住院的77例未接受HAART的HIV/AIDS患者外周血CD4+T淋巴细胞计数及血清隐球菌抗原阳性率,比较隐球菌抗原阳性患者与阴性患者的CD4+T淋巴细胞计数。结果 77例(男53例,女24例)中,血清隐球菌抗原阳性48例,阳性率为62.34%,阴性29例,阴性率为37.66%。其中,CD4+T淋巴细胞计数≤50个/mm3的患者血清隐球菌抗原阳性率为72.73%。血清隐球菌抗原阳性患者外周血CD4+T淋巴细胞计数显著低于阴性患者(P=0.022)。结论未接受HAART的外周血CD4+T淋巴细胞计数较低的HIV/AIDS患者血清隐球菌抗原阳性率高,血清隐球菌抗原阳性患者CD4+T淋巴细胞水平低于阴性患者(P<0.05)。     

云南地区HIV/AIDS患者合并深部真菌感染的类型及免疫学特征分析

目的分析云南地区近5年HIV/AIDS患者合并深部真菌感染的类型与免疫学特征。方法采集住院HIV/AIDS患者送检血液、脑脊液、骨髓、痰及脓液等标本进行菌种的培养及鉴定,用流式细胞技术检测合并深部真菌感染的HIV患者外周血T淋巴细胞亚群。结果 645例合并深部真菌感染的HIV/AIDS中分离出菌株702株,共11种,包括念珠菌304株(占43.3%),马尔尼菲篮状菌231株(占32.91%),新型隐球菌151株(占21.51%)。不同感染类型患者的CD4~+T淋巴细胞水平比较差异有统计学意义(χ~2=77.414,P0.01)。合并马尔尼菲篮状菌和新型隐球菌感染者的CD8~+T淋巴细胞水平比较差异有统计学意义(χ~2=33.624,P0.01)、马尔尼菲篮状菌与念珠菌感染的患者CD8~+T淋巴细胞水平比较差异有统计学意义(χ~2=13.161,P0.01)。HIV/AIDS患者合并感染真菌种类与CD4~+T/CD8~+T水平具有相关关系(χ~2=45.013,P0.01)。结论 HIV/AIDS患者易合并深部真菌感染,且不同菌种感染者的T淋巴细胞亚群构成不同。应根据艾滋病患者CD4~+、CD8~+ T淋巴细胞计数及CD4~+T/CD8~+T比值变化,进行治疗以降低病死率。     

HIV感染后不同病程阶段淋巴结中CD4~+T淋巴细胞各亚群频率的改变

目的评价淋巴结(lymph nodes,LNs)中初始、中枢记忆性、效应记忆性和效应CD4+T淋巴细胞频率的改变,探讨伴随疾病进展,LNs中CD4+T淋巴细胞各亚群频率改变的可能原因。方法选取HIV感染者71例,根据外周血CD4+T淋巴细胞计数和临床症状,将其分为HIV感染无症状组和AIDS患者组,并选取28例非HIV感染者作为健康对照组,留取以上3组LNs组织行活体组织检查,分离其中淋巴细胞,利用流式细胞术检测各细胞群的频率。结果①AIDS患者组LNs中初始CD4+T淋巴细胞频率与无症状组及健康对照组相比显著升高,而无症状组LNs中初始CD4+T淋巴细胞频率与健康对照组相比下降,但差异无统计学意义;②AIDS患者组LNs中中枢记忆性CD4+T淋巴细胞频率与健康对照组相比显著升高。虽然无症状组LNs中中枢记忆性CD4+T淋巴细胞频率与AIDS患者组相比下降,与健康对照组相比升高,但差异均无统计学意义;③AIDS患者组、无症状组和健康对照组LNs中效应记忆性CD4+T淋巴细胞频率依次升高,3组相比差异均有统计学意义;④无症状组、AIDS患者组和健康对照组LNs中效应CD4+T淋巴细胞频率依次降低,但3组相比差异均无统计学意义。结论 HIV感染后,LNs中初始、中枢记忆性、效应记忆性和效应CD4+T淋巴细胞频率并非随着疾病进展进行性下降。     

HIV/AIDS患者外周血CD4+ T细胞计数和淋巴细胞计数相关性的多中心研究

目的 对淋巴细胞计数预测CD4+ T细胞计数的准确性进行评价,为临床应用提供支持.方法 在全国23个分中心共筛查2 013例未接受抗病毒治疗的HIV/AIDS患者,经流式细胞术检测CD4+ T细胞计数,分析血常规当中的淋巴细胞计数和CD4+ T细胞计数之间的相关性,绘制ROC曲线判断淋巴细胞计数预测CD4+T细胞计数的准确性,并计算其敏感度、特异度、阳性预测值和阴性预测值.结果 2 013例HIV/AIDS患者的淋巴细胞计数和CD4+ T细胞计数分别为(1 600±670)×106/L和(244±148)×106/L,两者呈现显著正相关性(r=0.482,P＜0.000 1),以淋巴细胞计数预测CD4T细胞计数＜100×106/L、＜200×106/L和＜350×106/L的AUCROC分别为0.790(95％ CI0.761 ～0.818,P＜0.000 1),0.733(95％ CI0.710 ～0.755,P ＜0.000 1)和0.732 (95％ CI0.706 ～0.758,P ＜0.000 1).结论 在HIV/AIDS患者的临床诊治中,用淋巴细胞计数预测CD4+ T细胞计数有其实用价值,可以考虑在不具备CD4+ T细胞计数直接检测时作为替代指标用于监测疾病进展,也可以在获得CD4+ T细胞计数结果之前用来初步快速判断患者病情.     

人类免疫缺陷病毒感染者不同疾病阶段中自然杀伤细胞和γδT细胞的改变

目的 了解NK细胞和.γδT细胞在HIV/AIDS患者不同疾病阶段中的改变特点,探讨其在AIDS发病机制中的作用.方法 以311例未接受过抗HIV治疗的HIV/AIDS患者为研究对象,经流式细胞仪检测患者外周血CD4+T淋巴细胞、NK细胞和γδT细胞比例及计数,根据CD4+T淋巴细胞<0.20×109L、(0.20～0.35)×109L及>0.35×109/L.将病例分为低、中、高CD4+T淋巴细胞组,进行组间比较.Mann-WhitneyU检验和Kruskal-Wallis检验进行两组和多组独立样本秩和检验,Spearman和Pearson检验进行相关性分析.结果 HIV/AIDS患者外周血NK细胞比例及计数的中位数分别为8.4%和103×106L,γδT细胞比例及计数中位数分别为3.4%和41×106L,均明显低于健康对照组(Z=-5.029,Z=-7.723,Z=-2.437,Z=-6.063;均P<0.01).低、中、高CD4+T淋巴细胞组CD4+T淋巴细胞计数中位数分别为0.062×109L、0.276×109L、0.482×109L,NK细胞计数分别为89×106L、97×106L、146×106L,低、中CD4+T淋巴细胞组间的NK细胞计数差异无统计学意义,但均显著低于高CD4+T淋巴细胞组(Z=-3.392,P=0.001;Z=-4.849,P<0.01).低、中、高CD4+T淋巴细胞组γδT细胞计数中位数分别为29×106L、43×106L、59×106L,两两之间比较均差异有统计学意义(P<0.05).结论 HIV感染后外周血NK细胞和γδT细胞数量降低程度存在差异.     

Effect of irinotecan on the phenotype of peripheral blood leukocyte populations in patients with metastatic colorectal cancer

BACKGROUND/AIMS: Previous studies have demonstrated a significant decrease in absolute numbers of CD3+CD4+, CD8+CD28+ and CD19+ lymphocytes, and an increase in the expression of activation markers on T-cells and the number of CD14+CD16+ monocytes in patients with metastatic cancer. Irinotecan (CPT-11) is now being widely used for treatment of metastatic colorectal cancer patients. METHODOLOGY: We have examined, by two-color flow cytometry, peripheral blood leukocyte populations before and during systemic treatment with CPT-11 in 14 patients with metastatic colorectal cancer. RESULTS: CD3+, CD3+CD4+, CD3+CD8+, CD8+CD28+ and CD19+ were significantly lower, and CD3+HLA-DR+ and CD14+CD16+ cells were higher in metastatic colorectal cancer patients compared to controls. After 2-4 months of CPT-11-based chemotherapy, significant increase in CD3+CD4+ cell numbers was observed in 8 patients who had initial CD3+CD4+ counts of less than 600 per microL (358 +/- 154 vs. 652 +/- 319 cells per microL, Wilcoxon test, P < 0.01), while in patients with higher initial CD3+CD4+ counts a trend for decrease was observed during therapy. A trend for an increase in CD8+CD28+ cell counts was observed in patients with low CD3+CD4+ numbers, but no other changes were observed during the treatment in other peripheral blood leukocyte populations examined. CONCLUSIONS: CD3+CD4+ lymphocytopenia, a decrease in CD8+CD28+ and CD19+ lymphocytes, increased expression of activation markers and CD14+CD16+ monocytosis are present in a significant proportion of metastatic colorectal cancer patients. CPT-11-based therapy seems to ameliorate CD3+CD4+ lymphocytopenia, possibly by neutralizing the immunosuppressive effects of uncontrolled tumor growth. These observations may be useful for the design of immunotherapy trials in metastatic colorectal cancer.     

Effect of partial splenic embolization on the immune function of cirrhosis patients with hypersplenism

Objective:To discover the effect of partial splenic embolization on the immune function of cirrhotic patients with hypersplenism.Methods:Patients involved in the study were enrolled and divided into three groups,including control group,experimental group,and complication group.Numbers of CD3~+,CD4~+ and CD8~+ T cells and CD4~+CD25~+CDl27~(low/-) Treg cells in the peripheral blood of patients before surgery,1 month,6 months,1 year,and 2 years after surgery were analyzed by fluorescence active cell sorting(FACS).Contents of immunoglobulins(IgA,IgG and IgM) were analyzed by auto immunoassay analyzer.Results:In the peripheral blood of patients from experimental group,numbers of CD3~+,CD4~+ and CD8~+ T cells initially declined,but afterwards increased to normal level;in the peripheral blood of patients from complication group,CD3~+ and CD8~+ T cells showed the same trend,but the number of CD4~+ T cells was below normal level at all detection times.Furthermore,CD3~+,CD4~+ and CD8~+ T cells in the peripheral blood of patients from complication group were initially less than those in experimental group,and afterwards were comparable between two groups.In patients from both experimental group and complication group,the number of CD4~+CD25~+CDl27~(low/-)Treg cells increased 1 month and 6 months after surgery,and gradually restored to normal level.CD4~+CD25~+CDl27~(low/-)Treg cell counts in patients from complication group were initially more than those in patients from experimental group 1 month and 6 months after surgery,but then they were comparable.Furthermore,contents of immunoglobulins(IgA,IgG and IgM) were comparable in three groups at all detection times.Conclusion:Partial splenic embolization influenced the immune function of cirrhotic patients with hypersplenism in the short term but the immune function could afterwards gradually restore to normal.Our results implicated that measures that prevent infection and improve immune function were necessary in early stage after undergoing PSE in order to reduce complications.     

CD28-T细胞亚群在类风湿关节炎患者外周血和关节液中的表达

目的 探讨CD28-T细胞亚群在类风湿关节炎(RA)患者外周血和关节液中的变化和意义。方法 随机选择RA患者45例,取新鲜抗凝外周血单个核细胞(PBMC),其中15例同时提取关节液单个核细胞( SFMC),以流式细胞技术检测CD28-T细胞数量及其表面可诱导共刺激分子(ICOS)的表达。2 组间比较用独立样本t检验。结果 ①与PBMC相比,RA患者SFMC中CD4+CD28+ ICOS+、CD4+CD28-ICOS+、CD8+ CD28+、CD8+ CD28+ ICOS+T细胞明显升高[(36±19)％与(15±8)％,t=-4.234,P＜0.01;(2.1±2.2)％与(0.6±1.4)％,t=-3.143,P＜0.01;(62±15)％与(47±18)％,t=-2.885,P＜0.01;(9±9)％与(3±3)％,t=-2.131,P＜0.05];CD8+CD28-T细胞明显降低[(38±15)％与(54±18)％,t=2.975,P＜0.01];CD8+ CD28- ICOS+、C1D4+CD28+和CD4+CD28-T细胞无明显变化（P＞0.05）。②同一RA患者SFMC与PBMC相比,CD4+CD28+ICOS+、CD8+ CD28+T细胞明显升高[(38±18)％与(16±10)％,t=-4.065,P＜0.01;(61±16)％与(41±21)％,t=-2.883,P＜0.01];CD8+ CD28-T细胞明显降低[(39±16)％与(59±21)％,t=2.949,P＜0.01]。③缓解期与活动期RA患者相比,PBMC中CD4+CD28-、CD8+ CD28-、CD28-ICOS+T细胞无明显变化（P＞0.05）。结论 RA患者关节液中CD28-T细胞亚群失衡和ICOS分子表达异常,可能是导致RA关节损伤的重要机制。     

严重急性呼吸综合征患者淋巴细胞亚群的动态变化及意义

目的　了解成人严重急性呼吸综合征 (SARS)患者淋巴细胞亚群的改变对疾病的发生、发展及预后的影响。方法　依据卫生部颁发的传染性非典型肺炎临床诊断标准及预后将 2 0 6例SARS患者分为 3组 :即非重症组 13 3例、重症存活组 50例、重症死亡组 2 3例 ,用流式细胞仪进行淋巴细胞亚群CD4+、CD8+、CD19+、CD16+淋巴细胞的动态检测。建立数据库并对检测结果进行统计学分析。结果　SARS患者CD4+、CD8+、CD19+淋巴细胞计数均值分别是非重症组 >重症存活组 >重症死亡组 ,组间比较均P <0 .0 5。CD16+均值非重症组与重症存活组比较P >0 .0 5,两组与死亡组比较均P <0 .0 1。通过对CD4+、CD8+、CD19+、CD16+淋巴细胞计数动态观察 ,发现非重症组与重症存活组随病程CD4+、CD8+先下降后上升 ,CD19+随病程逐渐上升 ,CD 16+在发病早期有短暂的升高 ,然后波动在正常范围内。重症死亡组CD4+、CD8+、CD19+、CD16+在发病初期即处于较低水平 ,发病 15d后CD4+、CD8+、CD19+仍持续低水平 ,而CD16+随病程呈持续性降低。结论　成人SARS患者有明显的细胞免疫损伤 ,其淋巴细胞亚群的改变与临床分型及预后相关 ,对预后判断有一定的指导意义。SARS患者CD4+、CD8+淋巴细胞计数在发病初期是降低的 ,非重症组、重症存活组发病 9～15d是最     

Pro-atherogenic alterations in T-lymphocyte subpopulations related to acute hyperglycaemia in type 2 diabetic patients.

BACKGROUND: T cells are among the earliest cells to infiltrate the arterial intima during the initial stages of atherosclerosis. Alterations in the peripheral blood lymphocyte distribution might be associated with intensive lymphocytes extravasation and stimulation of atherosclerotic plaque development. Epidemiological data reveal that short-term postprandial hyperglycemia is a significant risk factor for coronary heart disease. Using a parameter that indicates recently-past acute hyperglycemia, 1,5-anhydro-D-glucitol (1,5-AG), the aim of the present study was to elucidate which alterations in peripheral blood T-lymphocytes, if any, are associated with acute hyperglycemia in patients with type 2 diabetes mellitus (DM) and, thus, might be involved in the progression of atherosclerosis. METHODS AND RESULTS: Measurement of fasting glucose level, glycated hemoglobin A(1c), 1,5-AG, lipid profile and lymphocyte receptors expression (CD3+, CD4+, CD8+, CD8+28+, CD+28 -) was performed in 97 patients with type 2 DM, 23 patients with coronary heart disease, and 15 healthy controls. The mean CD3+, CD4+, CD8+28 - and CD8+28+ lymphocyte counts were significantly higher in the DM patients than in both control groups. Multiple regression analysis revealed that CD4+ and CD8+28- lymphocyte counts primarily were dependent on 1,5-anhydro-D-glucitol plasma levels. CONCLUSIONS: These results suggest that acute hyperglycemia results in the progression of atherosclerosis in type 2 DM, at least in part through changes in CD4+ and CD8+28- lymphocyte subsets.     

Flow cytometry characterization of leukemic phase of nasal NK/T-cell lymphoma in tumor biopsies and peripheral blood

We report the findings of the immunophenotypic profile of three cases of nasal T/NK cell lymphoma in leukemic phase. Flow cytometry analysis was carried out using cell suspensions of tumor nasal biopsies and peripheral blood. Tumor samples were composed by a mixture of a predominant subset of medium-size true NK cytCD3epsilon-, sCD3epsilon-, CD56+ cells mixed with a minor subset of medium-size T/NK sCD3epsilon+, CD56+ cells. Both subsets were also detected in peripheral blood. In addition, an infiltration of small-size sCD3epsilon+, CD56- normal T lymphocytes was also present.     

Coexpression of CD4 and CD8 on peripheral blood T cells and lamina propria T cells in inflammatory bowel disease by two colour immunofluorescence and flow cytometric analysis.

Using two colour immunofluorescence with fluorescein isothiocyanate and phycoerythrin labelled monoclonal antibodies and multiparameter flow cytometry, we investigated the coexpression of CD4 and CD8 antigens on peripheral blood lymphocytes and lamina propria lymphocytes of patients with ulcerative colitis and Crohn's disease and normal control subjects. Both the absolute number and the proportion of peripheral blood CD4+, CD8+ cells in inflammatory bowel disease were small but significantly increased compared with those in normal control subjects. Peripheral blood lymphocytes activated with phytohaemagglutinin showed appreciably increased coexpression of CD4+, CD8+. These CD4, CD8 positive cells were large and granular. Thus the increased number of peripheral blood CD4+, CD8+ cells in inflammatory bowel disease suggests that chronic immune activation occurs not only in the active state of the disease but also in remission. The proportion of CD4+, CD8+ cells in the lamina propria was greater than in peripheral blood in normal subjects, suggesting chronic immune stimulation of the local immune system. This was also seen in patients with Crohn's disease or inactive ulcerative colitis. The proportion of CD4+, CD8+ cells was, however, significantly less in the lamina propria of patients with active ulcerative colitis. Whether this implies a possible defect in mucosal immunoregulation in active ulcerative colitis cannot be determined from these results.