共查询到20条相似文献,搜索用时 31 毫秒
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An initiation element in the yeast CUP1 promoter is recognized by RNA polymerase II in the absence of TATA box-binding protein if the DNA is negatively supercoiled
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Leblanc BP Benham CJ Clark DJ 《Proceedings of the National Academy of Sciences of the United States of America》2000,97(20):10745-10750
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The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5'' splice sites. 总被引:24,自引:3,他引:21
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P Zuo J L Manley 《Proceedings of the National Academy of Sciences of the United States of America》1994,91(8):3363-3367
ASF/SF2 is a human protein previously shown to function in in vitro pre-mRNA splicing as an essential factor necessary for all splices and also as an alternative splicing factor, capable of switching selection of 5' splice sites. To begin to study the protein's mechanism of action, we have investigated the RNA binding properties of purified recombinant ASF/SF2. Using UV crosslinking and gel shift assays, we demonstrate that the RNA binding region of ASF/SF2 can interact with RNA in a sequence-specific manner, recognizing the 5' splice site in each of two different pre-mRNAs. Point mutations in the 5' splice site consensus can reduce binding by as much as a factor of 100, with the largest effects observed in competition assays. These findings support a model in which ASF/SF2 aids in the recognition of pre-mRNA 5' splice sites. 相似文献
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Jung S. Byun Madeline M. Wong Wenwu Cui Gila Idelman Quentin Li Adriana De Siervi Sven Bilke Cynthia M. Haggerty Audrey Player Yong Hong Wang Michael J. Thirman Joseph J. Kaberlein Constantinos Petrovas Richard A. Koup Dan Longo Keiko Ozato Kevin Gardner 《Proceedings of the National Academy of Sciences of the United States of America》2009,106(46):19286-19291
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Mariano Alló Eneritz Agirre Sergey Bessonov Paola Bertucci Luciana Gómez Acu?a Valeria Buggiano Nicolás Bellora Babita Singh Ezequiel Petrillo Matías Blaustein Belén Mi?ana Gwendal Dujardin Berta Pozzi Federico Pelisch Elías Bechara Dmitry E. Agafonov Anabella Srebrow Reinhard Lührmann Juan Valcárcel Eduardo Eyras Alberto R. Kornblihtt 《Proceedings of the National Academy of Sciences of the United States of America》2014,111(44):15622-15629
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Bifunctional antisense oligonucleotides provide a trans-acting splicing enhancer that stimulates SMN2 gene expression in patient fibroblasts
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Skordis LA Dunckley MG Yue B Eperon IC Muntoni F 《Proceedings of the National Academy of Sciences of the United States of America》2003,100(7):4114-4119
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Shern L. Chew Hong-Xiang Liu Akila Mayeda Adrian R. Krainer 《Proceedings of the National Academy of Sciences of the United States of America》1999,96(19):10655-10660
Exonic splicing enhancers (ESEs) activate pre-mRNA splicing by promoting the use of the flanking splice sites. They are recognized by members of the serine/arginine-rich (SR) family of proteins, such as splicing factor 2/alternative splicing factor (SF2/ASF), which recruit basal splicing factors to form the initial complexes during spliceosome assembly. The in vitro splicing kinetics of an ESE-dependent IgM pre-mRNA suggested that an SF2/ASF-specific ESE has additional functions later in the splicing reaction, after the completion of the first catalytic step. A bimolecular exon ligation assay, which physically uncouples the first and second catalytic steps of splicing in a trans-splicing reaction, was adapted to test the function of the ESE after the first step. A 3' exon containing the SF2/ASF-specific ESE underwent bimolecular exon ligation, whereas 3' exons without the ESE or with control sequences did not. The ESE-dependent trans-splicing reaction occurred after inactivation of U1 or U2 small nuclear ribonucleoprotein particles, compatible with a functional assay for events after the first step of splicing. The ESE-dependent step appears to take place before the ATP-independent part of the second catalytic step. Bimolecular exon ligation also occurred in an S100 cytosolic extract, requiring both the SF2/ASF-dependent ESE and complementation with SF2/ASF. These data suggest that some ESEs can act late in the splicing reaction, together with appropriate SR proteins, to enhance the second catalytic step of splicing. 相似文献