HPLC法测定三种防风中升麻素苷及5-O-甲基维斯阿米醇苷含量

目的 建立防风中升麻素苷及5-O-甲基维斯阿米醇苷的测定方法,比较三种不同产地防风中有效成分的含量.方法 色谱柱为Apollo C18柱(250 mm×4.6 mm,5μm);柱温35℃;流动相甲醇:水(45:55);检测波长254 nm.结果 升麻素苷线性范围为y=1738044.84x-3442.10(R2=1),5-O-甲基维斯阿米醇苷线性范围为y=1981912.03x-2128.38(R2=1).结论 该方法检测结果峰型良好,分离度高,可用于同时测定防风中升麻素苷,5-O-甲基维斯阿米醇苷的含量.     

高效液相色谱法测定防风色原酮部位中4种成分含量

目的建立防风色原酮部位中4种成分升麻素苷、升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷的高效液相色谱含量测定方法。方法采用Agilent Zorbax C18柱(4.6 mm×150 mm,5μm),甲醇-水梯度洗脱,流速1.0 mL/m in,检测波长254 nm。结果升麻素苷平均回收率为102.56%、102.12%和95.50%,RSD分别为2.24%、1.33%和0.48%(n=5);升麻素平均回收率为95.66%、9.60%和97.54%,RSD分别为0.62%、1.23%和0.89%(n=5);5-O-甲基维斯阿米醇苷平均回收率为98.96%、96.76%和96.91%,RSD分别为0.48%、2.46%和2.74%(n=5);亥茅酚苷平均回收率为99.02%、103.40%和103.96%,RSD分别为2.51%、0.93%和1.61%(n=5)。结论3批样品测定结果表明,该方法简便、准确,可用于防风色原酮部位中升麻素苷等4种成分的含量测定。     

五虎散质量标准的研究

目的建立五虎散的质量标准。方法采用TLC法对处方中当归、白芷、红花进行定性鉴别；采用HPLC法测定升麻苷和5-O-甲基维斯阿米醇苷的含量。结果升麻苷在0．3700～1．8500μg，5-O-甲基维斯阿米醇苷在0．1910～0．9550μg与峰面积具有良好的线性关系；平均回收率分别为100．74％和98．33％，RSD分别为0．67％和1．91％(n=5)。结论建立的方法简便、准确、可靠，可作为控制该药品的质量标准。     

防风通圣丸中5-O-甲基维斯阿米醇苷含量测定方法的研究

目的:建立防风通圣丸中5-O-甲基维斯阿米醇苷含量的测定方法。方法:采用高效液相色谱法,C18柱(5μm,4.6mm×250 mm),以乙腈-0.1%磷酸溶液为流动相(梯度洗脱),流速1.0 mL/min,检测波长231 nm。结果:5-O-甲基维斯阿米醇苷在0.031 17μg~1.039 0μg范围内有良好的线性关系,测得平均回收率为97.32%,RSD为1.36%。结论:该方法简便、可靠、重现性好,适用于防风通圣丸的质量控制。     

高效液相色谱法同时测定玉屏风汤剂中6种成分

目的 建立以高效液相色谱法同时测定玉屏风散中2类化合物(黄酮类和色原酮类)6种有效成分升麻苷、升麻素、5-O-甲基维斯阿米醇苷、槲皮素、亥茅酚苷、芒柄花素含量的分析方法.方法 采用AgilentExtend-C18色谱柱(4.6mm×250mm,5 μm),柱温25℃;流动相为甲醇-1%乙酸水溶液,梯度洗脱,流速0.8ml/min;检测波长254 nm.结果 升麻苷、升麻素、5-O-甲基维斯阿米醇苷、槲皮素、亥茅酚苷、芒柄花素分别在90～1810 ng、97～1940 ng、190～1906 ng、105～3144 ng、88～2625 ng、109～3279 ng范围内标准曲线呈良好的线性关系,相关系数均在0.9999以上;平均加样回收率分别为98.2%,99.1%,97.3%,97.8%,98.8%,99.2%.结论 方法简便快速,结果准确,重复性好,线性范围宽,灵敏度高,可用于玉屏风制剂中升麻苷、升麻素、5-O-甲基维斯阿米醇苷、槲皮素、亥茅酚苷和芒柄花素含量的同时测定,为玉屏风散质量监控及新剂型研究提供了更加合理、可靠的检测方法.     

荆防抗感泡腾片的质量研究

目的建立荆防抗感泡腾片的质量标准。方法采用TLC法对处方中的药味进行定性鉴别;采用HPLC法测定升麻苷和5-O-甲基维斯阿米醇苷的含量。结果在TLC图谱中可以检出荆芥、防风等药味的特征斑点;升麻苷在71．2～427．2ng范围内,峰面积与进样量呈良好的线性关系,r=0．9992;中间精密度RSD为1．41％,平均回收率为100．37％（RSD=0．62％,n=6）;5-O-甲基维斯阿米醇苷在62．4～374．4ng范围内,峰面积与进样量呈良好的线性关系,r=0．9999,中间精密度RSD为1．41％,平均回收率为98．16％（RSD=1．38％,n=6）。结论所采用的质量研究方法准确,重复性良好,可以对本制剂的质量进行有效控制,确保制剂质量。      

HPLC测定小柴胡颗粒中柴胡皂苷、黄芩苷及甘草酸的含量

目的:建立以高效液相色谱法测定小柴胡颗粒中柴胡皂苷、黄芩苷和甘草酸含量的方法。方法:色谱柱为Kromasil C_(18),流动相为水(0.025%甲酸)-甲醇(0.025%甲酸),检测波长为250 nm。结果:柴胡皂苷在0.201 6μg/mL¹23.7μg/mL范围内线性关系良好(r=0.999 0),平均加样回收率为94.1%,RSD为1.62%(n=6)。黄芩苷在0.749μg/mL123.7μg/mL范围内线性关系良好(r=0.999 0),平均加样回收率为94.1%,RSD为1.62%(n=6)。黄芩苷在0.749μg/mL⁴58μg/mL范围内线性关系良好(r=0.999 5),平均加样回收率为95.2%,RSD为1.52%(n=6)。甘草酸在2.270μg/mL458μg/mL范围内线性关系良好(r=0.999 5),平均加样回收率为95.2%,RSD为1.52%(n=6)。甘草酸在2.270μg/mL¹35.5μg/mL范围内线性关系良好(r=0.999 5),平均加样回收率为94.8%,RSD为1.36%(n=6)。结论:该方法简便、灵敏、准确,可用于小柴胡颗粒的质量控制。     

蒲黄药材中香蒲新苷和异鼠李素-3-O-新橙皮苷含量测定方法优化研究

目的建立蒲黄中香蒲新苷和异鼠李素-3-O-新橙皮苷的含量测定方法。方法采用单因素考察方法建立供试品溶液制备方法;采用高效液相色谱法(HPLC)法测定香蒲新苷和异鼠李素-3-O-新橙皮苷的含量,色谱条件为Waters SunFire~(TM)C_(18)(4.6 mm×250 mm,5μm)色谱柱,乙腈-0.05%磷酸溶液梯度洗脱,流速1.0 m L/min,检测波长254 nm,柱温20℃。结果蒲黄药材采用80倍量的甲醇回流提取1.5 h,制备供试品溶液;香蒲新苷在0.245 2~1.471 0μg范围内线性关系良好,r=0.999 9,平均加样回收率为98.47%,相对标准偏差(RSD)=2.05%(n=6);异鼠李素-3-O-新橙皮苷在0.191 2~1.147 4μg范围内线性关系良好,r=0.999 7,平均加样回收率为100.56%,RSD=2.56%(n=6)。结论所建立的含量测定方法简便准确,重现性好,为蒲黄药材质量控制和评价奠定基础。     

RP-HPLC法测定山茱萸环烯醚萜总苷中马钱苷和莫诺苷含量

目的建立反相高效液相色谱法测定山茱萸环烯醚萜总苷中马钱苷和莫诺苷含量的方法,为完善山茱萸环烯醚萜总苷的质量评价提供依据。方法色谱柱:Lichrosphere C18柱(4.6mm×150mm,5μm);流动相:乙腈-水梯度洗脱(0~12min,乙腈比例由10%增加到55%);流速1.0mL/min;柱温30℃;检测波长240nm。结果马钱苷和莫诺苷分别在0.088~0.528μg(r=0.999 8)和0.088~0.352μg(r=0.999 5)之间范围内线性关系良好;平均回收率(n=6)分别为98.7%(RSD=1.3%)、98.6%(RSD=0.8%)。结论所建立的方法简便、准确、分离效果好、重复性好,可以用于控制山茱萸环烯醚萜总苷的质量。     

HPLC法测定桂枝茯苓丸有效部位中苦杏仁苷、芍药苷的含量

目的建立测定桂枝茯苓丸有效部位中苦杏仁苷、芍药苷质量分数的方法。方法采用Diamonsil C18(250 mm×4.6 mm,5μm)色谱柱;流动相为甲醇-水(体积比35∶65);流速为1.0 m L·min-1;检测波长为215 nm;柱温为30℃。结果苦杏仁苷和芍药苷的线性范围分别为0.354~7.088μg·m L-1(r=0.999 5)和0.356~7.128μg·m L-1(r=0.999 7),平均回收率分别为98.48%(RSD=1.47%)和99.06%(RSD=1.49%)。结论本方法专属性强、操作简便,适用于桂枝茯苓丸中有效部位的质量控制。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.