戊酸雌二醇对大鼠宫腔粘连形成的预防作用观察

目的 探讨雌二醇对大鼠宫腔粘连形成的预防作用及其机制.方法 30只雌性SD大鼠随机均分为5组,采用刮宫加感染双重损伤法制作大鼠宫腔粘连模型,实验处理因素为戊酸雌二醇灌胃.空白对照组(A)大鼠不予造模也不施加雌二醇处理;生理量雌激素组(B)造模但不给予雌二醇;低剂量雌激素组(C)、中剂量雌激素组(D)、高剂量雌激素组(E)于造模后分别给予剂量为0.206、0.514、1.028mg/(kg·d)的戊酸雌二醇灌胃.采用ELISA法测定各组大鼠戊酸雌二醇灌胃前后的血清雌二醇浓度,采用HE染色观察各组大鼠子宫内膜腺体数量,Masson染色计算子宫内膜纤维化面积,并通过免疫组化法检测子宫内膜组织中转化生长因子β(TGF-β)、基质金属蛋白酶9(MMP-9)的表达情况.结果 戊酸雌二醇灌胃后,C、D、E组血清雌二醇水平较A、B组明显增高(P＜0.05);随着灌胃戊酸雌二醇量增加,血清雌二醇水平也升高,差异有统计学意义(P＜0.05).与B组相比,C、D、E组子宫内膜腺体数目明显增多,纤维化面积比降低(P＜0.05),但D、E组间比较差异无统计学意义.与B组比较,C、D、E组子宫内膜TGF-β表达明显降低(P＜0.05),MMP-9表达明显升高(p＜0.05),但D、E组间差异无统计学意义.结论 雌二醇可减轻子宫内膜损伤后宫腔粘连的形成,在一定范围内随着雌二醇量的增加改善作用会更加明显.     

福建省平潭岛春季恙虫病疫源地现场标本的芯片检测及防制措施

目的：利用基因芯片技术对平潭岛春季恙虫病疫区的恙虫病疑似病人、宿主、媒介标本进行检测,证实疫源地的存在,采取有针对性的防制措施控制流行.方法：现场采集恙虫病疑似病人外周静脉血和捕鼠采集体表恙螨.根据Ot 56kD外膜蛋白基因序列,设计群特异性引物及探针,制备寡核苷酸芯片.PCR技术分别扩增样本Ot DNA片段,与芯片上的探针杂交,用荧光扫描仪检测并分析信号;加强卫生宣传,做好个人防护,搞好环境卫生,做好灭鼠、灭螨等防制措施.结果：对疑似病人血块3份、野鼠脾脏40份、恙螨5份DNA进行基因芯片检测,其中病人血3份,野鼠脾脏4份,恙螨2份检出阳性;经过2年防制,该地区2004年无恙虫病病例报告.结论：基因芯片检测样本具有特异、灵敏、快速的特点,从基因水平证实平潭岛春季恙虫病存在,所采取的防制措施切实有效.     

A method for chemical-shift-selective imaging

Selective suppression of a given resonance in an NMR image can be obtained with a pi/2 pulse whose excitation spectrum is zero at the appropriate chemical shift. The selective pi/2 pulse presented here is composed of two nonselective pi/2 pulses of opposite phase. The two pulses are separated by a short interval which determines the excitation spectrum.     

A screening method for knee trauma

RATIONALE AND OBJECTIVES: The authors performed this study to evaluate whether any one conventional radiographic view is sufficient as a screening method in the detection of acute knee trauma. MATERIALS AND METHODS: The authors prospectively assessed the efficacy of a single conventional radiograph of the knee in the detection of signs of knee fractures in 214 consecutive adults with acute knee trauma. The evaluated efficacy parameters included specificity, sensitivity, negative predictive value (NPV), and positive predictive value. The percentage reduction in the need for additional conventional radiographs was also calculated. RESULTS: Fifty-three of the 214 patients (24.8%) had a knee fracture. The sensitivity of the single lateral view in the detection of knee fractures was 100% (95% confidence interval [CI] = 94.3, 100). The lateral view of the traumatized knee was normal in 143 patients (66.8%). The probability of not having a fracture if the lateral view was normal (NPV) was also 100% (95% CI = 97.9, 100). The need for additional radiographs was reduced 67%. CONCLUSION: A single lateral view as a screening tool for knee fractures has a very high sensitivity and NPV. Because more than 65% of the patients had a normal lateral view in this study, there can be a considerable amount of savings in terms of radiology services for these patients.