125I标记抗CD20 HI 47 F(ab')2性质研究

目的 :对12 5I标记的抗CD2 0HI 4 7F(ab’) 2 性质进行研究。方法 :采用NBS法进行碘标 ,并测定标记物的标记率、免疫活性分数、放化纯度、亲和常数和体外稳定性。结果 :测定标记率为 87 4 % ,免疫活性分数为 0 4 0 8,放化纯度 >95 % ,亲和常数为 3 2× 10 9M-1,体外 4℃存放 5天 ,放化纯度降低至 0 2 1。结论 :标记物具有较高的放化纯度、免疫活性和稳定性 ,为体内治疗打下良好的基础。     

嵌合抗CD20抗体片段F(ab'')2的表达及活性

目的 :为了简化生产步骤 ,提高抗体蛋白的生物活性 ,探索在工程菌体内直接进行抗CD2 0F(ab’) 2 的高效率分泌性表达。方法 :采用单因素考察法优化培养条件 ,使蛋白G柱和S2 0 0 HR分子筛柱分离纯化目的蛋白 ,用MTT法检测抗CD2 0F(ab’) 2 抑制Daudi细胞体外生长的活性。结果 :发现用论文所选定的最适培养条件 ,抗CD2 0F(ab’) 2 的产量有了明显提高 ,从1 9～ 2 .2mg L达到了 3 7～ 4 3mg L ,F(ab’) 2 在表达产物中所占的比例也从 9 7%～ 13 2 %提高到了 38 1%～ 4 6 8% ;使用S2 0 0 HR分子筛柱对蛋白G柱亲和纯化后的产物进一步分离纯化 ,可以使F(ab’) 2 的纯度达到 85 %以上 ;MTT法检测结果证明F(ab’) 2 抑制Daudi细胞生长的IC50 值为 14 6 μg ml,而Fab’为 39 5 μg ml。 结论 :实现了抗CD2 0F(ab’) 2 在工程菌体内的高效率分泌性表达 ,而且所表达的抗CD2 0F(ab’) 2 比抗CD2 0Fab’具有更强的抑制Daudi细胞体外生长的能力     

碘标记肝癌单克隆抗体对裸鼠肝癌移植瘤导向治疗的意义和观察

本文报告用~(131)I标记肝癌单克隆抗体HAb18、及其F(ab’)2片段对裸鼠移植瘤导向治疗的观察,具有明显的杀伤效应,其效果优于国外同类抗体。 同位素标记与显像 用~(131)I标记HAb8、18IgG及其F(ab’)2片段,全部采用氯胺T碘化法,标记率:51％比活性:9.58×10~4Bq/μg。抗体与肝癌细胞在体外的亲和率:IgG—70％,F(ab’)2—55％。尾静脉注入碘标记物后,荷瘤裸鼠肿瘤部位明确显像。其定位指数;IgG—3.09,F(ab’)2—6.99。 实验分组与治疗12只荷瘤肝癌裸鼠(SMMU—LTNM)分4组,实验前服用0.25％     

疏水层析法和凝胶过滤法分离HI47抗CD20 F(ab'''')2之比较

目的　比较疏水层析和凝胶过滤两种方法分离纯化HI4 7抗CD2 0F(ab’) 2 的优缺点。方法　采用亲和层析柱protein G对发酵菌体裂解液进行粗纯化,分别采用疏水层析法和凝胶过滤法进行抗CD2 0F(ab’) 2 的分离纯化:疏水层析采用梯度洗脱,80 %B液洗脱2 0min ,10 0 %B液洗脱30min ;凝胶过滤以5 0mmol LPB缓冲液(pH 7.0 ) ,0 .8mL min洗脱4h。结果　粗纯化产物主要含F(ab’) 2 、Fab’,含量分别为19.6 %、5 9.7%。采用疏水层析法1h即可完成分离,F(ab’) 2 回收率为6 7% ,纯度为89.3% ;凝胶过滤4h完成分离,F(ab’) 2 回收率为6 5 % ,纯度为90 .5 %。FACS结果表明,两者结合Raji细胞的阳性率分别为99.93%和99.97%。结论　疏水层析分离纯化抗CD2 0F(ab’) 2 简单、快速、回收率高、纯度高,适用于大规模的工业化生产。     

疏水层析法和凝胶过滤法分离H147抗CD20F（ab’）2之比较

目的比较疏水层析和凝胶过滤两种方法分离纯化H147抗CD20F(ab’)2的优缺点。方法采用亲和层析柱protein-G对发酵菌体裂解液进行粗纯化，分别采用疏水层析法和凝胶过滤法进行抗CD20F(ab’)2的分离纯化：疏水层析采用梯度洗脱，80％B液洗脱20min，100％B液洗脱30min；凝胶过滤以50mmol／L PB缓冲液(pH7．0)，0．8ml/min洗脱4h。结果粗纯化产物主要含F(ab’)2、Fab’，含量分别为19．6％、59．7％。采用疏水层析法1h即可完成分离，F(ab’)2回收率为67％，纯度为89．3％；凝胶过滤4h完成分离，F(ab’)2回收率为65％，纯度为90．5％。FACS结果表明，两者结合Raji细胞的阳性率分别为99．93％和99．97％。结论疏水层析分离纯化抗CD20 F(ab’)2简单、快速、回收率高、纯度高，适用于大规模的工业化生产。     

抗血小板单克隆抗体片段的抗栓效应研究

目的 :研究抗血小板膜糖蛋白IIIa(GPIIIa)单克隆抗体 (mAb)SZ 2 1F(ab′) 2 片段的抗血栓效应。方法 :用木瓜蛋白酶在偏酸条件下 ( pH5 .5 )消化 2 0h ,经DEAE 5 2NaCl梯度洗脱消化产物 ,以得到F(ab′) 2 片段。收集产物经SDS PAGE鉴定 ,并通过ELISA和血小板聚集抑制试验 ,观察所获F(ab′) 2 片段免疫原活性和抗栓活性。结果 :该法制备F(ab′) 2 片段的得率为 6 0 .32 % ;酶免法测定其效价为 0 .8× 10 -9mol/L ;体外血小板聚集抑制半数有效剂量 (ED5 0 )为 3.395mg/L。F(ab′) 2 片段与血小板的结合性及对血小板聚集功能的抑制效果均高于完整的IgG。 结论 :利用木瓜蛋白酶制备的抗血小板mAbSZ 2 1的F(ab′) 2 片段 ,产率高且其抗栓活性与免疫原活性均优于完整的mAbIgG ,为mAbSZ 2 1用于临床抗血栓治疗展现了良好的前景     

用抗人胃癌单克隆抗体MG_7F(ab′)_2对胃癌病人进行放射免疫显像

本文采用DE 52纯化单抗,胃蛋白酶消化,Sephacryl S300分离,制备MG_4 F(ab’)_2,用~(13)I标记,对8例胃癌病人进行了RII 初步研究。 胃癌病人均经胃镜及病理确诊。接受检查前1～2天先用复方碘溶液封闭甲状腺,注射~(131)I-MG_7 F(ab’)_2前先静脉滴注地塞米松2～4mg,再缓慢滴注~(131)I-MG_7 F(ab’)_2 66.6-     

破伤风抗毒素制备方法的改进

目的:改进破伤风抗毒素的制备方法,提高破伤风抗毒素中F(ab’)2片段含量。方法:常规方法免疫马匹,通过延长胃酶消化时间、调整胃蛋白酶比例及pH值处理免疫血浆,经硫酸铵沉淀、明矾吸附、超滤及除菌后,测定F(ab’)2片段含量。结果:通过调整上述生产条件,破伤风抗毒素中F(ab’),含量与总蛋白比由60％增至90%。结论:改进后的方法提高了破伤风抗毒素F(ab’)2含量。     

单克隆抗体及其F(ab′)_2片段在小鼠体内的药物动力学及肿瘤显像研究

放射性同位素标记的抗肿瘤单克隆抗体(McAb)可用于体内肿瘤的定位显像及导向治疗。我们用~(125)I标记抗人小细胞肺癌单克隆抗体2F7和相应片段F(ab’)_2,比较它们在荷瘤裸鼠模型中的定位显像及经静脉(iv)或腹腔(ip)注射后在正常小鼠体内     

抗人结肠癌单克隆抗体CL_3全抗体与F(ab'''')_2片段在荷瘤裸鼠体内动态分布比较

为提高放射免疫显像(RAID)和放射免疫引导下外科手术(RIGS)的效果,用抗人结肠癌单克隆抗体(McAb)CL3,通过Pepsin消化法制备F(ab＇)_2,~(125)I标记,标记率73.1％,放化纯度99.5％,比活度4.7μCi/μg。结合分析法测定的免疫活性为56.8％,亲和常数为3.4×10~9L/mol。~(125)I-CL3全抗体标记率73％,放化纯度99.2％,比活度4.12μCi/μg,免疫活性     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

局部注射辛伐他汀对骨质疏松大鼠股骨髁骨小梁的改建效应

背景：局部注射具有成骨作用的辛伐他汀,可显著增加骨质疏松大鼠股骨颈及股骨髁部的骨密度及力学强度,分析局部注射辛伐他汀对股骨髁骨小梁的影响。 目的：进一步研究骨质疏松大鼠股骨内局部注射辛伐他汀对股骨髁骨小梁的影响。为将辛伐他汀应用于临床骨质疏松局部治疗提供实验基础。 方法：18只雌性SD大鼠双侧卵巢切除后3个月,制备大鼠骨质疏松模型。实验大鼠随机数字表法均分为3组,分别在实验大鼠的右侧股骨髓腔内单次注射辛伐他汀溶液5 mg、10 mg,对照组单纯注射空白载体。分别在注射后1个月处死大鼠并取材。Micro-CT扫描并定量分析骨组织形态变化。 结果与结论：给药后1个月,Micro-CT扫描结果显示,辛伐他汀治疗组的骨微结构参数如骨皮质厚度、骨小梁密度及连接率明显优于对照组。说明疏松骨骼单次注射小剂量辛伐他汀可显著促进股骨髁部骨小梁改建,改善骨骼微结构,可为强化局部、防治骨质疏松骨折的新选择进一步提供实验基础。     

石斛属民族药用植物的分类及生药学研究

中药石斛早在《神农本草经》中就被列为上品,其应用历史悠久,具有养阴生津、补肾益气、润喉护嗓、活血明目、抗癌防老等功效。本文对国内外石斛属民族药用植物的分类及生药学研究进行了综述。     

膝关节骨性关节炎的关节镜治疗体会

目的我科自2001～2005年4月对59例67膝的骨性关节炎（0A），进行关节镜检查及镜下清理术。方法镜检：膝关节骨性关节炎伴不同程度滑膜炎，滑膜皱壁粘连纤维片，关节软骨不同程度破坏。镜下清除增生滑膜，松解粘连，去除剥脱软骨、修复关节面。结果随访2月～4年，平均1.5年，术后综合评估2月～1年，优良率94％；1～2年，优良率79.2％；2年以上优良率54.8％。结论关节镜对膝关节骨性关节炎诊断能提供了比较全面的资料，并对骨性关节炎早期有良好疗效，具有创伤小、恢复快、并发症少和重复治疗等优点。     

Pathways of the realization of immunomodulating effects of neurotransmitters

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 401–404, April, 1993     

Anatomical study of distribution of valves of the cutaneous veins of adult''s limbs

Since the cutaneous veins of the four limbs have been used as autogenous grafts in the treatment of cardiovascular disorders, knowledge of distribution of the valves is increasingly required depending upon the use. In the gross anatomical study of distribution of valves of the trunci venae, there has been argument about locational relationships between the venous roots and the valves in the vicinity of the roots and the inter-valvular distance. However, there have been only few reports discussing detailed information about valves of the cutaneous veins of the four limbs. The authors observed patterns of distribution of the cutaneous venous valves of the four limbs of cadavers prepared for practice in anatomy. The following parts were excised from each cadaver: the cephalic, basilic, and the great saphenous veins, which originate from the acral venous network and flow into the proximal deep veins, and the venous roots communicating with these veins. An incision was made on each excised vein in the direction of the long axis under observation with a stereoscopic microscope, and the inter-valvular distance and the distance between the valve and the orifice of venous root in the vicinity of the valve were measured. The inter-valvular distance varied with type of the truncus venae, and it varied according to area even in the same truncus venae.     

Characterization of sterols of three digenetic trematodes of buffalo

Sterols of three digenetic trematodes were isolated and characterized by infrared and 1H nuclear magnetic resonance spectroscopies, and gas-liquid chromatography and gas chromatography-mass spectrometry. Sterols identified were cholesterol, cholestanol, 24-methylcholesterol, 24-methylcholestanol, 24-ethyl-22-dehydrocholesterol, 24-ethyl-22-dehydrocholestanol, 24-ethylcholesterol and 24-ethylcholestanol.     

Mechanisms of generation of slow components of bioelectric activity in diagnostics of functional state of the gastrointestinal tract

New electrophysiologic devices were used for the diagnostics of the state of excitable structures in the gastrointestinal tract and correction of their motor functions. Bioelectrical and biomechanical activities form the basis of functioning of internal organs. The mechanisms of generation of slow bioelectrical activity that are important for clinical and physiological studies are described. One of these mechanisms is a capacitance parametric transducer converting the energy of contractions into specific electric signals reflecting muscle functions. Another mechanism results from slow oscillations of resting potentials of interrelated excitable cells in large neuromuscular structures of internal organs. The elaborated procedure is efficient for preventing early postoperative paresis of the gastrointestinal tract. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 10, pp. 448–452, October, 1999     

胃小弯侧血管弓的MDCT解剖

目的：研究正常胃小弯侧血管弓及其属支血管的多排螺旋CT表现。方法：回顾性分析51例正常成人的上腹部CT资料,观察胃小弯侧血管弓及其属支血管的显示情况、血管弓形态及其与胃壁的关系。结果：51例均显示胃小弯侧血管弓,血管弓贲门部属支、胃体部属支和幽门部属支分别显示46例、15例和10例;血管弓与胃壁紧邻41例。结论：MDCT检查可良好显示正常胃小弯侧血管弓及其属支血管。     

Assessment of the efficacy of treatment of hemangiomas

Assessment of the aftereffects of cryoexposure and ultrahigh-frequency cryoexposure on hemangioma tissue of various types, cavernous and squamous, showed a higher cryogenic effect in hemangiomatous tissue preexposed to ultrahigh-frequency waves. A quantitative criterion is proposed for assessing the efficacy of the studied methods of exposure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 6, pp. 669–672, June, 1995