SLE患者CD45RA^＋和CD45RO^＋T细胞亚群的变化

利用流式细胞术及免疫双荧光染色法，检测３５例系统性红斑狼疮（ＳＬＥ）患者外周血ＣＤ４５ＲＡ和ＣＤ４５ＲＯＴ细胞亚群，结果表明：ＳＬＥ患者ＣＤ４细胞降低，ＣＤ８细胞增高，ＣＤ４／ＣＤ８降低，ＣＤ４ＣＤ４５ＲＡ细胞在病情活动期降低，在稳定期回升，ＣＤ４ＣＤ４５ＲＯ细胞在活动期有增高倾向，在稳定期下降，ＣＤ８ＣＤ４５ＲＡ及ＣＤ８ＣＤ４５ＲＯ细胞均在活动期增高，又均在稳定期降至正常水平，提示ＣＤ４５ＲＡ和     

抗CD4抗体不同地调节CD4,45RO^＋和CD4,45RA^＋T细胞的辅助性…

观察了抗ＣＤ４抗体（ａｎｔｉ－ＣＤ４）在体外对ＣＤ４Ｔ淋巴细胞亚群ＣＤ４，４５ＲＡ＾＋和ＣＤ４，４５ＲＤ＾＋Ｔ细胞的辅助性功能及Ｔ细胞受体调节的细胞内Ｃａ＾２＋＿信号的影响，ａｎｔｉ－ＣＤ４主要是通过影响Ｔ－Ｂ淋巴细胞相互作用早期而影响ＣＤ４，４５ＲＤＴ细胞的辅助性功能，ａｎｔｉ－ＣＤ４对ＴＣＲ／ＣＤ３调节的Ｔ细胞增殖反应的抑制是由于抑制了细胞内Ｃａ＾２＋信号的传导，并且ＣＤ４５的分子结构密切相关     

IL—4在诱导CD4,45RA^＋和CD4,45RO^＋T细胞向IL—4产生细胞分化？…

应用ＥＬＩＳＰＯＴ（Ｅｎｚｙｍｅ－ｌｉｋｅｄｉｍｍｕｎｏｓｐｏｔａｓｓａｙ）单细胞检测技术，研究了淋巴因子在诱导ＣＤ４，４５ＲＯ和ＣＤ４，４５ＲＡＴ细胞向ＩＬ－４产生细胞分化过程中的影响，在存在ＰＭＡ和ｉｏｎｍｙｃｉｎ的情况下，ＩＬ－２和ＩＬ－４均可以诱导ＣＤ４，４５ＲＯＴ细胞分化为ＩＬ－４产生细胞，但是只有ＩＬ－４可以诱导ＣＤ４，４５ＲＡＴ细胞向ＩＬ－４产生细胞分化，ＩＦＮγ既不能诱导ＣＤ４，４     

分泌细胞因子的CD8^＋T细胞亚群研究进展

Ｔ细胞具有功能不同的亚群，其中ＣＤ４＾＋辅助Ｔ细胞（ＴＨ）的亚群分类及功能早已成定论。近几年的研究发现传统的ＣＤ８＾＋细胞毒Ｔ细胞（ＣＴＬ）也可分泌与ＣＤ４＾＋Ｔ细胞相似的细胞因子，也可分为两类亚群，分别定义为Ｔｃ１和Ｔｃ２。本文就近年来这方面的研究进展作一综述。     

CD4~＋T细胞亚群T_H1、T_H2与实验性自身免疫病

ＣＤ４￣＋Ｔ细胞在调节免疫系统的功能上起着重要作用，一旦这些调节过程发生之错，就可能引起器官司特异性自身免疫病。ＣＤ４＋Ｔ细胞的一个特殊亚群，即Ｔ＿Ｈ１细胞与器官司特异性自身免疾病的发生有关，而Ｔ＿Ｈ２细胞则能防止此类疾病的发生。     

普通变异型免疫缺陷病病例报道

<正> 普通变异型免疫缺陷病(CVID)Com-mon Variable Immunodeficiency Disease又名迟发型低丙种球蛋白血症:是儿童时期常见的原发性免疫缺陷病之一,现报道我们近年来发现的4例,以期引起重视。 病例介绍 例1 女,11岁(431811)83年2月确诊,主诉为不规则发热10年,反复呼吸道感染4年。既往史:生后3个月起反复高效、出血点及肝脾肿大,拟诊为“恶网”勒雪氏病。经强的松等治疗,热退、肝脾缩小。3岁时二腮腺肿大持续,曾拟诊为“结节病”,以后仍不时发生。4岁时发热、皮疹、红斑肿胀,拟诊“红斑狼疮”?皮肌炎。半月后消失。6岁后反复低热、咳嗽,疑为肺结核,抗痨治疗1年未愈,确诊为肺     

CD4^＋T细胞亚群TH1,TH2与实验性自身免疫病

ＣＤ４＾＋Ｔ细胞在调节免疫系统的功能上起着重要作用，一旦这些调节过程发生差错，就可能引起器官特异性自身免疫病。ＣＤ４＾＋Ｔ细胞的一个特殊亚群，即ＴＨ１细胞与器官特异性自身免疫病的发生有关，而ＴＨ２细胞则能防止此类疾病的发生。     

T细胞受体信号转导中的CD45亚型的研究进展

T细胞表面的T细胞抗原受体(TCR)拥有结合抗原的能力,当抗原以抗原肽主要组织相容性复合物(MHC)的形式出现时,两者结合形成TCR-抗原肽-MHC复合物从而启动T细胞信号,进而在T细胞内发生一系列信号级联反应.白细胞共同抗原CD45分子既是作为细胞膜上信号转导的关键分子,也是T细胞中最丰富的蛋白质酪氨酸磷酸酶,在TC...     

BCGF对普通变异型免疫缺陷病激活B细胞增殖的影响

普通变异型免疫缺陷病(Common Variable im-munodificiency CVID)是儿童时期常见的原发性免疫缺陷病,其特点为低免疫球蛋白(Ig)血症和反复感染,发病机理复杂,部分病例B 细胞完全缺如,大     

滤泡辅助性T细胞的特征及其在自身免疫病和免疫缺陷病中的意义

淋巴滤泡是T细胞辅助B细胞体液免疫的重要场所,趋化至该部位的滤泡辅助性T细胞具独特的表型、基因型和功能,是一特殊的CD4+T细胞群体。该类细胞的异常可能与某些自身免疫病和免疫缺陷病相关。     

CD45RA／CD45RO临床研究进展

目的：探讨CD45RA／CD45RO的研究现状从而有效的指导临床工作。方法：查阅近年来国内外CD45RA／CIM5RO的相关文献，对其最新研究进行总结。结果：作为跨膜酪氨酸磷酸蛋白酶，CIM5RA／CD45RO在哺乳动物淋巴细胞信号传导、增殖和活化中有重要作用，是区分初始和记忆T细胞的重要标志。结论：CD45RA／CD45RO的检测在人类自身免疫疾病和病毒感染性疾病的诊断方面有重要意义。     

CD45RA and CD45RO isoforms in infected malnourished and infected well-nourished children.

The aim of this study was to determine if the distribution in vivo of CD4(+)CD45RA(+)/CD45RO(-) (naive), CD4(+)CD45RA(+)/CD45RO(+) (Ddull) and CD4(+)CD45RO(+) (memory) lymphocytes differs in malnourished infected and well-nourished infected children. The expression of CD45RA (naive) and CD45RO (memory) antigens on CD4(+) lymphocytes was analysed by flow cytometry in a prospectively followed cohort of 15 malnourished infected, 12 well-nourished infected and 10 well-nourished uninfected children. Malnourished infected children showed higher fractions of Ddull cells (11.4 +/- 0.7%) and lower fractions of memory cells (20.3 +/- 1.7%) than the well-nourished infected group (8.8 +/- 0.8 and 28.1 +/- 1.8%, respectively). Well-nourished infected children showed increased percentages of memory cells, an expected response to infection. Impairment of the transition switch to the CD45 isoforms in malnourished children may explain these findings, and may be one of the mechanisms involved in immunodeficiency in these children.     

重症肌无力CD45RA、CD45RO胸腺细胞亚群及相关细胞因子表达分析

目的:探讨胸腺细胞异常分化与重症肌无力发生的关系.方法:采用基因芯片对多种白细胞介素、干扰素及其受体mRNA表达进行分析, 采用流式细胞术测定胸腺细胞CD45RA、 CD45RO的表达率, 采用免疫组化对胸腺组织切片CD45RA、 CD45RO表达及分布进行检测.结果:MG患者IL-1R、 IL-4R、 IFNγR1、 IFNγR2、 IL-6、 IL-8表达水平显著低于对照组; IL-10RB表达水平显著高于对照组; IL-1、 IL-2、 IL-4、 IL-10、 IL-7、 IFN-α、 IFN-β、 IFN-γ表达水平在MG和对照组均很低, 无显著差异; MG患者CD56 胸腺细胞百分率(0.56±0.33)显著低于对照组(1.78±0.69), MG患者CD45RO 、 CD1a 细胞显著高于对照组.免疫组化和RT-PCR也有相同结果.结论:MG患者胸腺细胞发育过程中CD45RO 细胞向CD45RA T细胞转变存在异常.     

慢性乙型肝炎患者外周血CD45RA+、CD45RO+T淋巴细胞亚群的特点及临床意义

目的：探讨慢性乙型肝炎患者外周血CD4^＋CD45RA^＋、CD4^＋CD45RO^＋、CD8^＋CD45RA^＋和CD8^＋CD45RO^＋T淋巴细胞亚群的特点及其与肝病病情的关系。方法：采集46例轻中度慢性乙型肝炎患者、58例重度慢性乙型肝炎患者和30例健康人的外周抗凝血，应用流式细胞技术三色荧光分析法对其外周血中CD4^＋CD45RA^＋、CD4^＋CD45RO^＋、CD8^＋CD45RA^＋和CD8^＋CD45RO＋T淋巴细胞亚群进行检测。结果：轻中、重度慢性乙型肝炎患者与正常人相比，其外周血中CD4^＋、CD8^＋T细胞均无明显改变；CD8^＋CD45RA^＋T细胞均明显降低，CD8^＋CD45RO^＋T细胞均明显增高，而CD4^＋CD45RA^＋、CD4^＋CD45RO^＋T细胞均无明显改变；重度慢性乙型肝炎患者与轻中度慢性乙型肝炎患者相比，CD8^＋CD45RA^＋T细胞明显降低（P〈0.05），CD8^＋CD45RO^＋T细胞明显升高（P〈0.05）。结论：乙型肝炎慢性化过程中，CD8^＋CD45RO^＋T细胞起重要作用且与慢性乙型肝炎患者病情的进展呈正相关；检测CD4^＋CD45RA^＋、CD4^＋CD45RO^＋、CD8^＋CD45RA^＋和CD8^＋CD45RO^＋T淋巴细胞亚群比检测CD4^＋和CD8^＋T细胞亚群更能正确、充分、全面地了解慢性乙型肝炎的发病机制和预后，从而有效地指导临床治疗。     

Hyper-reactivity of mouse CD45RA − T cells

Mouse CD4 T cells have been partitioned into CD45RA and CD45RA^? subpopulations by means ot the monoclonal antibody 14.8. The CD45RA^? subpopulation proliferated more actively and generated more interleukin-4 (IL-4) in response to stimulation with anti-CD3 antibody and phytohemaglutinin, and more IL-2 in response to anti-CD3. This subpopulation is therefore hyper-reactive to these polyclonal stimulators, but does not show the bias towards T helper type 2 activity that has been found in studies with other related CD45 isoforms. No evidence of suppression was obtained by comparing proliferation of CD45RA^? cells in the presence and absence of CD45RA cells. Thus mouse CD4 T cells behave in these respects similarly to those of man, as is evident in a brief review of the quiescence-activation-quiescence cycle in the two species.     

慢性乙型病毒性肝炎患者外周血T淋巴细胞CD27和CD45RA表达的研究

目的分析慢性乙型病毒性肝炎患者外周血T淋巴细胞CD27和CD45RA的表达。方法采集分离健康人和慢性乙型病毒性肝炎患者外周血单个核细胞(PBMC),利用多种荧光标记抗体标记细胞表面分子,再用流式细胞仪检测CD8+T淋巴细胞表面CD27和CD45RA表达情况。结果31例慢性乙型病毒性肝炎患者CD8+CD45RA+CD27+T细胞占CD8+T细胞(29.03±13.18)%,低于28例健康对照组的(60.85±14.36)%,P<0.01。而CD8+CD45RA-CD27+T细胞占CD8+T细胞(30.31±24.11)%,显著高于健康对照组的(10.32±5.24)%,P<0.05。慢性乙型病毒性肝炎患者CD4+CD45RA+CD27+T细胞21.12±9.64%低于健康对照组的(60.89±17.93)%,P<0.01,而CD4+CD45RA-CD27+T细胞(54.28±18.75)%显著高于健康对照组的(27.16±9.24)%,P<0.01。结论健康人外周血CD8+和CD4+T淋巴细胞均以CD45RA+CD27+初始细胞表型为主,而慢性乙型病毒性肝炎患者外周血初始细胞减少,CD45RA-CD27+表型的T淋巴细胞明显增加。     

Comparison of lymphokine secretion and mRNA expression in the CD45RA+ and CD45RO+ subsets of human peripheral blood CD4+ and CD8+ lymphocytes

Flow cytometric analysis of human peripheral blood T lymphocytes demonstrated that the majority of the CD4⁺ cells were CD29⁺ or CD45RO⁺ “mature” cells while the CD8⁺ cells were primarily CD45RA⁺ “naive” cells. After an initial separation into CD4⁺ and CD8⁺ cells and a secondary separation into CD45 subsets, lymphokine secretion was assessed after phorbol 12-myristate 13-acetate and ionomycin or fixed anti-CD3 stimulation. Within the respective CD45 subsets, CD4⁺ cells produced more interleukin (IL)-2, IL-4, and IL-6; but the CD8⁺ cells secreted more interferon-γ and granulocyte/macrophage-colony-stimulating factor. Tumor necrosis factor-α secretion was similar in the matched CD45 subsets. Northern analysis revealed a parallel pattern of lymphokine mRNA expression in the four lymphocyte subsets. These results suggest that human CD8⁺ peripheral blood lymphocytes have a significant capacity to secrete lymphokines, and that the low lymphokine production observed in unseparated CD8⁺ cells reflects the higher percentage of less functional CD45RA⁺ cells.     

Expression in vivo of CD45RA,CD45RB and CD44 on T cell receptor-transgenic CD8+ T cells following immunization

We used mice transgenic for a major histocompatibility complex class I-restricted T cell receptor to study the changes of phenotype in vivo which follow priming by antigen of CD8 T cells. We show that following priming with peptide, CD44 on CD8 T cells is up-regulated. The change of phenotype was relatively stable, as primed CD8 cells isolated from thymectomized mice 6 weeks after priming still expressed increased levels of CD44. CD8 T cells in these mice are still responsive to peptide and could represent long-lived primed cells. No downregulation in vivo of the CD45RA or CD45RB isoforms was found, indicating that there is a differential regulation of the expression of CD44 and CD45RB by activated CD8 transgenic T cells. These results contradict earlier studies in vitro which showed that CD8 T cells which have been primed earlier belong to the CD45RA^? or CD45RB^? subset.     

Co-expression of the CD45RA and CD45RO antigens on T lymphocytes in chronic arthritis

The site of T lymphocyte activation in chronic arthritis is unknown. Peripheral blood (PB) lymphocytes from chronic arthritis patients are in a ‘naïve’ or non-activated state, as defined by expression of the CD45RA antigen and lack of HLA class II expression. In contrast, most synovial fluid (SF) T lymphocytes express a ‘memory’ or activated phenotype, as defined by the CD45RO antigen and high HLA class II expression. Following stimulation, naive cells lose CD45RA and gain CD45RO expression to become memory cells with a transitional stage of dual CD45RA, CD45RO antigen expression. To localize where this change in phenotype occurs we used dual colour immunofluorescence labelling to compare the percentage of dual CD45RA, CD45ROpositive T lymphocytes in PB and SF from chronic arthritic patients and from normal PB, assuming this population would be increased at the primary site of T lymphocyte activation. Expression of the intermediate and late activation marker. HLA-DR, was also analysed using dual colour immunofluorescence labelling. The percentage of dual positive T lymphocytes was similar between arthritic PB, SF. and normal PB, as was the density of both CD45RA and CD45RO antigens. Thus, CD45 isoform expression did not indicate where T lymphocytes were activated. However, we identified a previously unreported population of CD45RA⁺ CD45RO⁺ HLA-DR^- T lymphocytes in arthritic and normal PB. In SF, this population was absent, but a substantial number of dual CD45RA, CD45RO-positive HLA-DR⁺ T lymphocytes were identified. This population would not be predicted by the current model of T lymphocyte activation. Division of T lymphocytes into functional groups on the basis of CD45 isoform expression is likely to be more complicated than previously thought. Based on our findings we propose an alternative model of T lymphocyte differentiation.