四氯化碳诱发大鼠肝纤维化自发逆转中库普弗细胞与星状细胞的分布

目的:探讨四氯化碳(CCl_4)诱发大鼠肝纤维化自发逆转过程中肝库普弗细胞与星状细胞的分布和意义.方法:500 mL/L CCl_4腹腔注射8 wk诱发大鼠肝纤维化模型,实验第8,12周末检测血清生化指标,观察肝组织的病理变化,采用免疫组化SP法观察单核巨噬细胞抗原(ED1),α-平滑肌动蛋白(α-SMA)阳性表达的肝库普弗细胞(kupffer cell,KC)和肝星状细胞(hepatic stellate cells,HSC)的分布结果:第8周末模型组大鼠与对照组比较,血清ALT和AST活性(568.18±630.46 nkat/L vs 472.26±167.37 nkat/L.P<0.05:5845.84±1353.27 nkat/L vs 1698.51±663.30 nkat/L.P<0.01),肝/体质比(3.90±0.85 vs 2.56±0.24,P<0.001)及胶原纤维面密度(5.87±1.13 vs 0.52±0.30,P<0.001)明显增高;大量ED1和α-SMA阳性的KC和HSC主要分布在汇管区增生的纤维组织及纤维间隔内.第12周末模型组与第8周比较大鼠血清ALT活性(1020.70±306.73 nkat/L vs 376.74±304.06 nkat/L.P<0.05)仍较高外,胶原纤维面密度减少,汇管区增生的纤维组织及纤维间隔内ED1阳性的KC减少,α-SMA阳性的HSC消失.结论:腹腔注射CCl_48 wk后大鼠肝功能明显损伤,形成肝硬化,KCs激活和HSCs活化相关,停止注射CCl_4 4 wk后大鼠肝纤维化发生自发逆转.     

银杏叶提取物对大鼠肝纤维化Activin A表达的影响

目的研究银杏叶提取物（EGB）对肝纤维化大鼠Activin A表达的影响及抗纤维化作用。方法2004-10-2005-4武汉大学人民医院消化内科将30只雄性大鼠随机分为3组：正常组、模型组及EGB干预组。模型组及EGB干预组给予四氯化碳（CCl4）腹腔注射造模，干预组同时每天给予EGB灌胃。8周后处死动物，取肝脏液氮冻存及甲醛固定，行HE染色，免疫组化及RT-PCR检测Activin A的表达。结果光镜下组织学检查纤维化分级EGB干预组低于模型组（P〈0．05），免疫组化及RT-PCR检测Activin A的表达EGB干预组低于模型组（P〈0．01）。结论EGB对CCl4诱导的大鼠肝纤维化有良好的防治作用，作用机制可能与肝组织Activin A表达减少有关。     

中药护肝灵对实验大鼠乙醇分解酶的影响

目的：探讨中药护肝灵对乙醇代谢相关酶的影响．方法：以乙醇灌胃法复制大鼠模型,分别以易善复及高、低剂量护肝灵处理,双波长法检测血中乙醇浓度,按试剂盒说明书检查肝匀浆中CAT含量,活性分光光度法检测肝匀浆ADH含量,荧光实时定量RT-PCR观察ADH和CYP450ⅡE1的mRNA表达．结果：与正常对照组相比,模型组血清乙醇含量显著升高(545．7±l08．85mg/L vs -51．4±2．83mg/L,P＜0．05),ADH和CAT含量显著降低(667±272nkat/L vs 1884±650nkat/L,P＜0．05：112±29nkat vs 255±43nkat,P＜0．05)．与模型组相比,中药护肝灵可以降低口服乙醇大鼠血中乙醇含量(低剂量：248．5±96．63mg/L；高剂量：-0．1±33．94mg/L；均P＜0．01),提高肝组织ADH(低剂量：1184±422nkat/L：高剂量：1550±458nkat/L；均P＜0．05)和CAT(低剂量：202±31nkat/L；高剂量：167±34nkat/L；均P＜0．05)含量,造模后肝组织CYP450ⅡE1 mRNA显著下降(10±2 vs 15±2,P＜0．01),护肝灵可使肝组织CYP450ⅡE1 mRNA表达进一步下降(高剂量：8±2,P＜0．05)．造模及药物处理后,ADH mRNA均无显著变化(P＞0．05)．结论：中药护肝灵可激活乙醇代谢相关酶,进而促进乙醇在体内的代谢．     

银杏叶提取物对大鼠肝纤维化Activin A表达的影响

目的研究银杏叶提取物(EGB)对肝纤维化大鼠Activin A表达的影响及抗纤维化作用。方法2004-10~2005-4武汉大学人民医院消化内科将30只雄性大鼠随机分为3组:正常组、模型组及EGB干预组。模型组及EGB干预组给予四氯化碳(CCl4)腹腔注射造模,干预组同时每天给予EGB灌胃。8周后处死动物,取肝脏液氮冻存及甲醛固定,行HE染色,免疫组化及RT-PCR检测Activin A的表达。结果光镜下组织学检查纤维化分级EGB干预组低于模型组(P<0·05),免疫组化及RT-PCR检测Activin A的表达EGB干预组低于模型组(P<0·01)。结论EGB对CCl4诱导的大鼠肝纤维化有良好的防治作用,作用机制可能与肝组织Activin A表达减少有关。     

银杏叶提取物与秋水仙碱对大鼠肝纤维化预防作用的比较

目的比较银杏叶提取物(GBE)和秋水仙碱对实验性大鼠肝纤维化的预防作用。方法SD雄性大鼠40只随机分为4组:正常组(n=10)、模型组(n=10)、秋水仙碱预防组(n=10)及GBE预防组(n=10)。模型组、秋水仙碱预防组及GBE预防组给予500 mL/L CCl4腹腔注射,1 mL/kg,每周2次,共8周,秋水仙碱预防组每天同时给予秋水仙碱灌胃,0.2 mg/kg,GBE预防组每天同时给予GBE灌胃,0.3 g/kg。实验结束后,心脏取血分离血清行肝功能生化指标检测,处死动物取肝脏甲醛固定,常规行HE染色,免疫组化检测α-SMA和TGF-β1。结果光镜下组织学检查纤维化分级GBE预防组低于秋水仙碱预防组(P0.05),肝功能生化指标检测GBE预防组优于秋水仙碱预防组[ALT:(168.4±34.6)U/Lvs(210.6±40.8)U/L;AST:(318.8±62.5)U/Lvs(511.2±53.2)U/L;ALB:(31.0±2.1)g/Lvs(28.1±2.0)g/L;P均0.05],免疫组化检测α-SMA及TGF-β1蛋白表达GBE预防组低于秋水仙碱预防组(α-SMA:29.3±1.5vs5.1±2.2;TGF-β1:14.5±0.9vs28.6±0.9)。结论GBE预防大鼠肝纤维化的作用优于秋水仙碱,GBE作为一种新的抗肝纤维化药物有很好的研究和应用前景。     

腹腔注射左旋精氨酸诱导急性坏死性胰腺炎大鼠模型

目的:通过大剂量左旋精氨酸(1-arginine) ip诱导大鼠急性胰腺炎(acute necrotizing pancreatitis,ANP)模式,探讨此模型制备的方法和机制．方法:将60只SD大鼠随机分为正常对照组(C组,n=24),ANP模型组(A组,n=36)．C组接受生理盐水对照;A组用60 g/L左旋精氨酸分3次ip建立ANP模型．分别观察两组大体病理变化、光镜下病理评分、血清淀粉酶水平．结果:1-arginine ip后24 h可观察到集中于小叶边缘区的大片胰腺组织坏死、腺小叶结构消失,A组的病理评分(4 h:4．45±1．33 vs 0．50±0．55．P<0．01;12 h:5．33±1．66 vs 0．67±0．82．P<0．01;24 h:7．89±1．67 vs 0．67±0．82,P<0．01;36 h:8．33±1．12 vs 0．67±0．82, P<0．01)、血清淀粉酶(4 h:8296．16±1028．21 nkat／L vs 6315．10±816．83 nkat／L,P<0．01;12 h: 11 255．92±2565．18 nkat／L vs 5867．84±632．29 nkat／L,P<0．01;24 h:54 424．22±27 125．09 nkat／L vs 6078．05±1070．88 nkat／L．P<0．01; 36 h:28 494．53±12 278．62 nkat／L vs 6286．26±1074．38 nkat／L,P<0．01)各时点都比C组明显升高．结论:采用分3次ip 60 g／L左旋精氨酸方法可成功诱导ANP模型．     

清肝活血方对乙醇性肝纤维化大鼠肝星形细胞和肝细胞凋亡的影响

目的:观察酒精性肝纤维化大鼠Hsc和肝细胞的凋亡及中药清肝活血方对细胞凋亡的影响.方法:以乙醇为主制备酒精性肝纤维化大鼠模型,将造模大鼠分为清肝活血方低(4.75g/kg)、中(14.25g/kg)和高剂量组(28.5g/kg),每日进行ig药物干预2wk,并设空白对照组、模型对照组及易善复对照组.比色法检测血清ALT,AST,γ-GT;HE和Masson染色观察肝组织炎症和纤维化程度.TUNEL检测肝细胞凋亡,TUNEL-α-SMA双标记检测HSC凋亡.结果:清肝活血方用药组及易善复组能降低大鼠血清ALT(1213±245,1432±253nkat/Lvs2140±428nkat/L,P均<0.05),AST(1671±400,2123±413vs4454±850nkat/L,P均<0.05),γ-GT水平(4539±1847,5509±2430vs8271±3304nkat/L,P均<0.05),减轻纤维化程度(5.5±2.50,6.30±3.16vs9.00±2.27,P<0.05);诱导活化的HSC凋亡(5.25%±2.48%,3.63%±2.04%vs2.30%±1.24%,P<0.05),减少肝细胞凋亡(0.43%±0.11%,0.60%±0.16%vs1.77%±0.49%,P<0.05).结论:清肝活血方能有效减轻大鼠肝纤维化程度,并减少乙醇引起的肝细胞凋亡,诱导活化的HSC凋亡.     

ASC在大鼠胰腺炎肺损伤发病中的作用及中药清胰汤对其表达的影响

目的:探讨ASC(apoptosis-associated specklike protein containing a Caspase recruitment domain)在胰腺炎肺损伤发病机制中的作用及中药清胰汤对胰腺炎肺损伤大鼠ASC及其相关的细胞因子表达的影响．方法:SD大鼠40只,随机分4组:假手术组、胰腺炎肺损伤组、清胰汤治疗组、善宁治疗组．采用15 g／L去氧胆酸钠逆行注入胰胆管诱发胰腺炎肺损伤模型．通过自动生化分析仪检测大鼠血清淀粉酶;采用ELISA法测定血清 IL-1β水平;RT-PCR检测肺组织ASC mRNA的表达;免疫组织化学法检测肺组织和胰腺组织ASC蛋白的表达．通过检测肺组织髓过氧化物酶(MPO)活性、肺湿／干质量比值和肺组织病理切片判定肺损伤程度．结果:胰腺炎肺损伤组血清淀粉酶和IL-1β水平较假手术组显著升高(77 632±5934 nkat／L vs 16 303±1450 nkat／L,P<0．01和386．26± 50．54 ng／L vs99．11±18．43 ng／L,P<0．01)．清胰汤治疗组血清淀粉酶(17 420±1867 nkat／L)和 IL-1β(105．23±20．21 ng／L)水平较肺损伤组血清淀粉酶(77 632±5934 nkat／L,P<0．01)和IL- 1β水平(386．26±50．54 ng／L,P<0．01)明显下降．善宁治疗组血清淀粉酶(20 437±123 nkat／L) 和IL-1β水平(109．63±19．98 ng／L)较肺损伤组血清淀粉酶(77 632±5934 nkat/L,P<0．01)和 IL-1β水平(386．26±50．54 ng／L,P<0．01)明显下降．假手术组肺组织内ASC mRNA表达较弱．胰腺炎肺损伤组肺组织及胰腺组织内ASC蛋白表达较假手术组显著上调,其灰度值有显著性差异(24．86±5．23 vs 54．12±7．91,P<0．01和 25．46±4．21 vs 52．32±8．10,P<0．01),清胰汤治疗组两者表达显著下调(48．97±7．45vs24．86 ±5．23,P<0．01和49．48±8．13vs25．46±4．21, P<0．01)．善宁治疗组两者的表达亦显著下调 (48．69±5．87 vs 24．86±5．23．P<0．01和49．11± 6．41vs25．46±4．21,P<0．01)．清胰汤治疗组和善宁治疗组肺组织MPO活性减低(5．33±0．50 nkat／L vs 18．67±1．17 nkat／L,P<0．01和5．16± 0．83 nkat／L vs 18．67±1．17 nkat／L,P<0．01),肺湿／干质量比值下降(7．02±0．34 vs 9．98±0．47, P<0．01和6．78±0．33vs9．98±0．47,P<0．01),肺组织损害程度明显减轻．结论:ASC在大鼠胰腺炎肺损伤发病机制中起到一定的作用,中药清胰汤通过下调ASC及其相关细胞因子IL-1β的表达起到治疗胰腺炎肺损伤的作用．     

大鼠急性胰腺炎时胰腺腺泡细胞凋亡及Bax,Caspase-8的表达

目的:研究大鼠急性胰腺炎腺泡细胞凋亡情况,凋亡调控基因Bax与凋亡蛋白酶Caspase-8的表达及其同疾病严重程度的关系.方法:胆胰管逆行注入不同浓度去氧胆酸钠方法制备大鼠急性胰腺炎模型,设假手术(SO)组、急性水肿性胰腺炎(AEP)组和急性坏死性胰腺炎(ANP)组.采用TUNEL技术检测胰腺腺泡细胞的凋亡,RT-PCR与免疫组化方法分别分析胰腺组织Bax,Caspase-8 mRNA及蛋白的表达,对胰腺组织进行病理学评分并测定血清淀粉酶及IL-6,TNF-α含量.结果:与SO组比较,AEP与ANP组胰腺组织显示不同程度的病理损害,血清淀粉酶(63 413±6118,1 532 134±17 654 nkat/L vs 15 736±483 nkat/L,P＜0.01)及TNF-α(1.86±0.13,2.97±0.14μg/L vs 0.95±0.08 μg/L,P＜0.01),IL-6 (47.10±7.05,170.10±7.59 ng/L vs 29.20±4.47 ng/L,P＜0.01)浓度显著升高,且ANP组显著高于AEP组(P＜0.05);AEP与ANP组腺泡细胞凋亡指数显著升高(22.09±3.71,6.50±1.58 vs 0.13±0.05,P＜0.01),但ANP组显著低于AEP组(P＜0.05).同时发现,AEP与ANP组胰腺组织Bax (mRNA:1.530±0.501,1.046±0.337;蛋白:453.7±30.5,339.4±26.7)和Caspase-8(mRNA:0.595±0.17,0.505±0.173;蛋白:3606±337,3134±231) mRNA及蛋白表达水平均显著高于SO组(Bax mRNA:0.613±0.244,Bax蛋白:245.2±30.0;Caspase-8mRNA:0.357±0.130,Caspase-8蛋白:2396±266)(P＜0.01),ANP组Bax mRNA及蛋白表达水平显著低于AEP组(1.046±0.337,339.4±26.7 vs 1.530±0.501,453.7±30.5,P＜0.05),ANP组Caspase-8 mRNA及蛋白表达水平低于AEP组,但差异不具有显著性意义.结论:急性胰腺炎时胰腺腺泡细胞发生凋亡伴随凋亡调控基因Bax与凋亡蛋白酶Caspase-8表达增加,并同病情严重程度呈负相关关系.     

丹芍化纤胶囊对肝纤维化大鼠PDGFR-β和p-ERK1/2的影响

目的通过观察丹芍化纤胶囊对肝纤维化大鼠PDGFR-β和p-ERK1/2的影响,探索其抗肝纤维化的可能机制.方法♂SD大鼠55只,体质量180-220 g,随机分为正常组、模型组、预防组.除正常组外,其余均采用四氯化碳、高脂饮食及乙醇复合因素复制肝纤维化模型,造模同时预防组每日一次予以0.8 g/kg丹芍化纤自来水悬液灌胃,模型组、正常组予以等体积自来水灌胃,持续8 wk.造模结束后肝组织HE染色病理检查,免疫组化检测肝组织PDGFR-β、蛋白印迹检测肝组织p-ERK1/2在各组表达,生化法检测各组血清透明质酸(HA)、层黏蛋白(LN)、Ⅲ型前胶原(PⅢP)、白蛋白(ALB)、总蛋白(TP),计算白球比(A/G).结果造模8 wk后经病理学证实模型大鼠形成典型的肝纤维化,模型成功.与正常组相比,模型组肝组织PDGFR-β、p-ERK1/2及血清HA、LN、PⅢP均有显著升高,ALB、A/G显著降低(PDGFR-β1 84.6±8.5 vs 89.6±5.8,P＜0.05;p-ERK1/2360.0±14.5 vs 15.4±2.1,P＜0.05;HA517.5±91.5 μg/L vs 254.4±33.1 μg/L,P＜0.05;LN58.4±11.3 μg/L vs 37.3±9.8 μg/L,P＜0.05;PⅢP36.9±5.6 μg/L vs 4.7±1.5 μg/L,P＜0.05;ALB27.4±4.9 g/L vs 42.1±1.6 g/L,P＜0.05;A/G0.89±0.08 vs 1.38±0.09,P＜0.05);与模型组相比,预防组肝组织PDGFR-β、p-ERK1/2及血清HA、LN、PⅢP均有显著降低,ALB、A/G显著升高(PDGFR-β91.1±6.3vs 184.6±8.5,P＜0.05;p-ERK1/2253.8±18.2vs 360.0±14.5,P＜0.05;HA322.9±41.4 μg/Lvs 517.5±91.5 μg/L,P＜0.05;LN46.0±9.4μg/L vs 58.4±11.3 μg/L,P＜0.05;PⅢP14.5±2.4 μg/L vs 36.9±5.6 μg/L,P＜0.05;ALB37.2±2.8 g/L vs 27.4±4.9 g/L,P＜0.05;A/G1.18±0.13 vs 0.89±0.08,P＜0.05).结论PDGFR-β及p-ERK1/2在肝纤维化形成中起重要作用,丹芍化纤胶囊具有良好的预防肝纤维化形成的作用,其降低PDGFR-β及p-ERK1/2在肝组织的表达可能是其作用机制之一.     

青少年高血压的研究进展

随着人们生活和行为方式的改变,高血压发病明显呈年轻化趋势。在青少年时期识别高血压病高危人群有助于早期进行有效干预和治疗,降低未来高血压的发生率及其严重性。现试从青少年高血压的诊断、发病因素、特点、治疗策略等方面的研究进展作一综述。     

Morbidity in cardiovascular diseases in immigrants in Sweden

INTRODUCTION: Although immigration to Sweden has increased in the last few decades, the incidence rates of cardiovascular disease and coronary heart disease in immigrants are unknown. The aim of the present study is to estimate whether place of birth affects the incidence rates of cardiovascular disease and coronary heart disease. MATERIAL AND METHODS: The study was designed as a follow-up study on morbidity in cardiovascular disease and coronary heart disease between 1 January 1997 and 31 December 1998, including three and a half million persons with age range 35-64 years, of whom 550 000 were born abroad, from the database MigMed consisting of the whole Swedish population. Incidence rates and relative risks were estimated by indirect standardization and a proportional hazard model. RESULTS: The age-adjusted risk of coronary heart disease was higher in most foreign-born groups than in Swedes. For example, in nine of 12 male groups, the relative risks varied between 1.1 and 2.2, and in seven of 12 female groups, the relative risks varied between 1.4 and 2.5. When also adjusting for level of education and employment status, the risks were still high, but on a lower level. CONCLUSIONS: Foreign-born people possess an over-risk of cardiovascular or coronary heart disease(CVD/CHD) compared with Swedish-born persons, also when level of education and employment status are taken into account.     

C·肉毒杀鼠素(冻干剂)应用研究

目的为研究C·肉毒杀鼠索对杀灭达乌尔黄鼠(简称黄鼠)的大面积应用情况和对家畜、家禽的毒害作用,进行了C·肉毒杀鼠素的应用研究.方法大面积投毒采用ES-2药饵撒播机[1],间隔约80m进行条投.羊、鸡采用直接灌胃.结果大面积应用的灭鼠率为83.72%.对羊、鸡最高剂量分别为500万MLD、150万MLD,均未出现中毒现象.结论 C·肉毒杀鼠素是较为理想的草原大面积杀灭黄鼠的理想、首选药物.     

高龄老年高血压的临床研究进展

随着社会老龄化进程的加快,80岁以上高龄老人的绝对数量以及占总人口的比例均在增加,如何提高高龄老年高血压的防治水平备受关注。现试从高龄老年高血压的临床特点、治疗策略等方面的临床研究进展作一综述。     

中国老年性耳聋的研究与干预进展

老年性耳聋已成为影响我国老年人生活质量的最主要的慢性病之一。助听器是目前帮助听力损失的老年人克服交流障碍的主要手段。在我国,数字助听器已逐渐取代模拟助听器并且体现出了更好的效果。但是老年听力损失患者中使用助听器的比例仍然很小。人工耳蜗植入也已被应用在老年患者中。我国针对老年人的听力康复服务还有较长的路要走。     

Sex Differences in Ethanol-induced Dopamine Release in Nucleus Accumbens and in Ethanol Consumption in Rats

In vivo microdialysis was used to examine changes in nucleus accumbens and striatal dopamine, dihydrophenylacetic acid (DO-PAC), and homovanillic acid (HVA) following acute administration of ethanol (0.0, 0.25, 0.5, 1.0, or 2.0 g/kg) in male and female Long-Evans rats. Following dialysis, rats were trained to bar-press for oral ethanol reinforcement. In nucleus accumbens, females showed significant increases in extracellular dopamine following 0.25 or 0.5 g/ kg ethanol, but did not show significant increases over baseline at the higher doses. Males showed slight increases in dopamine at the lower doses and decreased dopamine at 2.0 g/kg. In striatum, both sexes showed increased dopamine at the lower doses and decreased dopamine at 2.0 g/kg. There were slight increases in nucleus accumbens DOPAC and HVA at some doses in both sexes, but no changes in striatal metabolite levels. In addition to showing increased responsiveness to ethanol-induced mesolimbic dopamine stimulation, females consumed more ethanol than males during behavioral testing. The pattern of both greater ethanol-induced nucleus accumbens dopamine release and greater ethanol consumption in females supports the hypothesis that ethanol reward is mediated, at least in part, by the mesolimbic dopamine system.     

Epstein-Barr virus in malignancies in renal transplant recipients in Japan

A role for Epstein-Barr virus (EBV) in the development of malignancies including lymphomas, and carcinoma of the stomach, nasopharynx, thymus and salivary gland is suggested. It is indicated that EBV evokes polyclonal-B-cell-proliferative diseases in immunocompromised hosts, such as transplant patients, which results in monoclonal malignant lymphomas. The suppression of immune functions in these patients is thought to lead to incomplete elimination of the cells expressing EBV latent infection genes. To examine the etiological role of EBV in the development of malignancies following renal transplant in Japan, 42 malignancies in 1744 cases of renal transplant were studied for the presence and type of EBV. The polymerase chain reaction revealed that 5 malignancies were positive for EBV, all type A: 2 of 2 cases of non-Hodgkin's lymphoma (NHL), 2 of 8 cases of gastric adenocarcinoma of the common type, and 1 of 2 cases of gastric plasmacytoma. In situ hybridization revealed positive signals in the nucleus of tumor cells in 2 cases of NHL and 1 of plasmacytoma. Positive signals were found in the small lymphoid cells but not in the tumor cells in 2 cases of gastric carcinoma. On the basis of these findings, a role for EBV in the development of malignancies in renal transplant patients is unlikely except for lymphoid neoplasias.Abbreviations PCB polymerase chain reaction - EBV Epstein-Barr virus - NHL non-Hodgkin's lymphoma     

糖尿病大鼠心室肌细胞钾通道的改变

目的研究糖尿病大鼠心室肌细胞钾通道的改变及增加葡萄糖代谢对其的作用。方法取体重150～200g的雄性SpragueDawley大鼠,腹腔注射链脲菌素(STZ)建立糖尿病模型,采用酶解法获得单个心室肌细胞,应用膜片钳全细胞记录技术记录钾电流。结果糖尿病大鼠心室肌细胞瞬间外向性钾流(Ito)密度较对照组显著降低[ 60mV时,分别为(15.90±1.19)pA/pF(n=25)和(28.55±0.97)pA/pF(n=12),P<0.001];分别用100nmol/L胰岛素及1.5mmol/L二氯乙酸在体外预处理心室肌细胞4～5h和3～4h使Ito密度恢复至对照组水平[ 60mV时,分别为(29.40±0.38)pA/pF(n=20)和(27.35±0.97)pA/pF(n=12)]。结论糖尿病大鼠心室肌细胞钾通道功能发生改变,增加葡萄糖代谢可逆转这一改变,提示葡萄糖代谢与Ito功能间存在一定关系。