Simulation and analysis of the electromyographic interference pattern in normal muscle. Part II: Activity, upper centile amplitude, and number of small segments

We have defined three new features of the electromyographic (EMG) interference pattern (IP): activity, upper centile amplitude (UCA), and number of small segments (NSS). These parameters were measured in simulated IPs constructed by adding together motor unit action potentials (MUAPs) recorded with a concentric needle EMG electrode. The activity increases linearly with the number of MUAP discharges to approximately 80% of its theoretical maximum value. The UCA correlates strongly with the peak-to-peak amplitude of the largest MUAP in the IP and the mean segment amplitude and does not depend on the discharge rate of the largest MUAPs. We infer that the UCA defines the upper limit of the peak-to-peak amplitude of the MUAPs contained in the IP. The NSS increases with the number of MUAP discharges, but reaches a constant value at higher MUAP discharge rates, probably because small amplitude MUAPs are masked by the large amplitude MUAPs. The potential value of these parameters in automated IP analysis is discussed.     

Automatic analysis of the electromyographic interference pattern. Part I: Development of quantitative features

We have developed three new features of the electromyographic interference pattern (IP), based on the turns and amplitude of the signal, to quantitate some of the features of the IP that are usually assessed subjectively by an electromyographer. The activity measures the fullness of the IP. The upper centile amplitude (UCA) defines the upper limit of the maximum peak-to-peak amplitude of the motor unit action potentials (MUAPs) contained in the IP. The number of small segments (NSS) measures the complexity of the IP, which is a reflection of the polyphasicity of the component MUAPs. The activity and the logarithm of the UCA correlate strongly with the force of muscle contraction at which the IP is measured. The NSS initially increases with the force of contraction and becomes relatively constant at higher force levels. The normal values of these features and the interpretation of their relationships are described in companion papers.     

Automatic analysis of the electromyographic interference pattern using the turns: amplitude ratio

This study was performed to compare different techniques of analyzing the electromyographic interference pattern (IP). Recordings were made from the biceps muscle with a concentric needle electrode at different sites and at different constant levels of voluntary contraction. The number of turns per second (NT), the mean amplitude change between successive turns (MA) and NT:MA ratio were determined for epochs of 1 sec duration. Normal limits of individual epoch NT:MA ratios and the mean value of NT:MA ratio obtained from all epochs in each muscle were determined. The mean NT:MA ratio was less in normal males than in females. IP recordings were made in the biceps muscle of 69 patients with neuropathy and 54 patients with myopathy, though this muscle was not necessarily affected by the disease in all patients. The IP was abnormal by visual inspection in 82% of patients compared to 61% based on NT:MA ratio and 74% using a technique that automatically quantitates some features of the IP that are assessed subjectively by an electromyographer. All techniques demonstrated IP abnormalities in more than 80% of the muscles that were moderately to severely weak. Though measuring the NT:MA ratio without monitoring the force of contraction is not as sensitive as other IP analysis techniques, it may be useful in quantitating abnormalities when other techniques are not available.     

Automatic analysis of the electromyographic interference pattern. Part II: Findings in control subjects and in some neuromuscular diseases

The electromyographic (EMG) interference pattern (IP) was measured in the biceps muscle of 16 normal male and 17 normal female subjects. The activity, upper centile amplitude (UCA), and the number of small segments (NSS) (defined in a companion paper) were measured from 500-msec epochs of the IP. The normal values of these features were defined separately for men and women by plotting the UCA and NSS values against activity for each epoch and defining an area on these plots, called a “cloud,” that contained more than 90% of the datum points from each study. The mean deviation of the individual datum points from the overall mean values was also calculated for each study. A study in one muscle is considered to be normal if more than 90% of the datum points from that muscle are within the normal clouds and the deviation values are within their normal range. In patients with neuropathy, the characteristic pattern was increased UCA with normal or decreased NSS. In patients with myopathy, NSS was increased and the UCA was normal or decreased. In all studies, the interpretations of the IP from the plots agreed with qualitative assessments of the IP made independently by an electromyographer. The use of these features to understand and quantitate the changes in the motor units produced by disease is demonstrated by serial studies performed in a patient with motor neuron disease.     

Turns amplitude analysis of the orbicularis oculi and oris muscles.

OBJECTIVE: The aim of the study was to determine whether 'clouds' from turns amplitude analysis obtained from the orbicularis oculi and oris muscles without force monitoring can be used to differentiate pathological processes affecting the face. METHODS: The interference pattern from orbicularis oculi and orbicularis oris was studied using a concentric needle electrode. Data-points from 20 normal subjects were plotted on a logarithmic scale of mean amplitude between turns versus turns/second, from which linear regression analysis defined the 95% confidence intervals. This enabled us to draw the boundaries of the normal cloud on a linear plot. Data-points from the interference pattern in two pathological cohorts, of 6 patients receiving botulinum toxin injections (representing a neurogenic model), and 6 patients with a muscle dystrophy (representing a myopathic model) were plotted against the normal cloud. These findings were compared and correlated with the mean durations obtained on motor unit action potential analysis from these same two facial muscles. RESULTS: The majority of patients receiving botulinum toxin injections into their facial muscles showed a pattern of high amplitude with low turns/s, or low amplitude with a low-to-normal range of turns/s in both facial muscles. These findings were associated with high-duration motor unit action potentials in most cases. In the myopathic group of patients 66% showed a pattern of low amplitude with low-to-normal range of turns/s in O oculi and O oris. This correlated with short-duration motor unit action potentials in both facial muscles. CONCLUSIONS: We have demonstrated that turns amplitude analysis without force monitoring can be used to study the interference pattern from facial muscles and can be applied to differentiate primary neurogenic from myopathic pathological processes. SIGNIFICANCE: Turns amplitude analysis without force monitoring in the facial muscles can be used as an effective and practical method of interference pattern analysis to complement findings from conventional motor unit action potential analysis.     

Turns-amplitude analysis of the electromyographic recruitment pattern disregarding force measurement. I. Method and reference values in healthy subjects.

We used turns-amplitude analysis to characterize the EMG recruitment pattern disregarding force measurement. The electrical muscle pattern of the brachial biceps (BB), abductor pollicis brevis (APB), medial vastus (MV), and anterior tibial (AT) muscles was analyzed during progressive increase in force from rest to maximum using the mean amplitude as an indicator of the force of the muscle. The following parameters were obtained on-line: the maximal ratio of turns to mean amplitude (peak-ratio, PR), the mean amplitude, and the number of time intervals (TI) between turns at PR and at near maximum force (NMF). The highest PR values were obtained in BB, the lowest in MV. Analysis of the distribution of the TI between turns at different degrees of voluntary contraction showed fewer spikes with short duration and small amplitude at high force compared with low force.     

Computed tomography in affective disorders. Part I. Ventricular and sulcal measurements

Sixty patients with affective illness were examined by computed tomography (CT) and compared to 60 age- and sex-matched controls. Using three different methods of measurement, no differences in ventricular brain ratio (VBR) between patients and controls could be detected. However, linear ventricular values of the frontal horns plus the bicaudate distance (Huckmann number) and the third ventricle were enlarged in the total group of patients. This was also found in subgroups of older, male, psychotic, unipolar, Dexamethasone Suppression Test (DST) positive, and lithium-treated patients when compared to individually matched controls. Younger, nonpsychotic, female, bipolar, DST-normal and non-lithium-treated patients did not differ from controls. The Huckmann number correlated with the duration of illness, as measured by the first episode and the number of episodes. The comparison between the subgroups of patients yielded higher linear and VBR values in older subjects, in men, and in psychotic subjects, whereas no differences were found in comparisons between unipolar versus bipolar, DST-positive versus DST-negative, and lithium-treated versus non-lithium-treated patients.     

Cervical nerve root stimulation. Part I: technical aspects and normal data.

OBJECTIVE: Cervical nerve root stimulation (CRS) is a technique of assessing the proximal segments of motor axons destined to upper extremity muscles. Few studies report normal values. The objective was to determine CMAP onset-latencies and CMAP amplitude, area, and duration changes in healthy controls for the abductor pollicis brevis (APB), abductor digiti minimi (ADM), biceps, and riceps muscles. In addition, to determine the tolerability of CRS, as measured by the visual analog scale (VAS). METHODS: We studied 21 healthy volunteers prospectively with CRS using four target muscles (APB, ADM, biceps, and triceps) bilaterally. Collision studies were used in all APB recordings. VAS was obtained in all subjects. RESULTS: Mean CMAP onset-latencies were: APB 14 +/- 1.5 ms; ADM 14.2 +/- 1.5 ms; biceps 5.4 +/- 0.6 ms; triceps 5.4 +/- 1.0 ms. Onset-latency significantly correlated with height for all nerves. The mean change in CMAP amplitude and area (%) between most distal stimulation and CRS was: APB reduction of 15.1 +/- 11.6 and 4.9 +/- 3.6%; ADM reduction of 21.1 +/- 10.7 and 17.2 +/- 8.8; biceps reduction of 10 +/- 11.5 and reduction of 8.7 +/- 6.8; triceps increase of 3.3 +/- 5.2 and 11.0 +/- 9.9% respectively. Mean CMAP duration change between most distal stimulation and CRS was: APB, increase of 20.4 +/- 7.4%; ADM, increase of 14.4 +/- 8.5%; biceps, increase of 13.9 +/- 10.8%; triceps, increase of 7.7 +/- 6.7%. The mean VAS score was 3.8 +/- 1.2, and all subjects completed the study. CONCLUSIONS: The present study establishes normative data and indicates that CRS is a well-tolerated technique. SIGNIFICANCE: The normal values may be used as reference data for the needle CRS technique in the assessment of proximal conduction abnormalities.     

Peak-ratio interference pattern analysis in the detection of neuromuscular disorders

Peak-ratio interference pattern analysis (peak-ratio method) is said to have a high sensitivity and to be independent of sex and age. This study was carried out to prove or disprove these findings. The peak-ratio method and qualitative motor unit action potential (MUAP) analysis were applied to the right brachial biceps and anterior tibial muscles of 44 healthy subjects, aged 23–87 years, 25 neuropathy patients, aged 21–83 years, and 29 myopathy patients, aged 19–70 years. Peak-ratio parameters were independent of sex and age. They tended to be lower in the anterior tibial muscle than in the brachial biceps muscle. Neuropathy patients typically showed decreased peak-ratio, short time intervals and increased amplitude/turn. Myopathy patients typically showed increased peak-ratio, turns/s and short time intervals. The sensitivity of the peak-ratio method was 72% for neuropathy patients and 59% for myopathy patients. The sensitivity of the peak-ratio method was similar to that of the MUAP analysis in neuropathy patients and higher than that of the MUAP analysis in myopathy patients. The specificity of the peak-ratio method was 80%. The peak-ratio method proved to be a valuable, supplementary electromyographic tool for the detection of neuromuscular disorders.     

Turns-amplitude analysis of the electromyographic recruitment pattern following upper motor neuron lesions

OBJECTIVE: To assess the pattern of motor unit recruitment of weak muscles in upper motor neuron (UMN) lesions. MATERIALS AND METHODS: Ten patients underwent turns-amplitude analysis (TAA) on the paretic and healthy brachial biceps muscles, in the acute and subacute stages of hemiparesis. The control group comprised 10 age- and sex-matched subjects. RESULTS: Although absent in the acute stage, five patients developed a myogenic cloud pattern in their paretic extremities in the subacute stage; which was statistically significant when compared with controls (P = 0.033). Mean amplitude was reduced in both acute and subacute stages of the hemiparesis compared with controls (P = 0.000). The turns/mean amplitude ratio in the subacute stage was increased compared with both the paretic limbs examined in the acute stage (P = 0.000) and to controls (P = 0.000). CONCLUSION: Abnormalities in the recruitment of motor units in UMN lesions give rise to a myogenic cloud pattern in the TAA, which is prominent in the third month after the initial insult. This may result from the increase in motor unit activity, while the recruitment of bigger motor units is still defective.     

Pattern of electrical activity and force in normal and pathological muscle: S-index of turns and amplitude

Cenkovich et al. (1982) found that a rotated hyperbolic curve fitted the relationship between the logarithm of cumulative amplitude (log A) and the logarithm of turns (log T). They found the intercept of the hyperbola (S-index) to be independent of force and suggested to use the S-index without measurement of the force as a diagnostic criterion. We have applied this modification of the method to our material previously published. In controls we found a slight increase in S-index with increasing force. The S-index was often increased in patients with myopathy and decreased in patients with neurogenic disorders, mostly at low to moderate force. The S-index was not better in discriminating patients from controls than the ratio of turns to mean amplitude.     

Developing normal number of small segments-activity clouds of the electromyography interference pattern

Guillain-Barré syndrome (GBS) is an inflammatory polyradiculoneuropathy associated with numerous viral infections. Recently, there have been many case reports describing the association between coronavirus disease-2019 (COVID-19) and GBS, but much remains unknown about the strength of the association and the features of GBS in this setting. We reviewed 37 published cases of GBS associated with COVID-19 to summarize this information for clinicians and to determine whether a specific clinical or electrodiagnostic (EDx) pattern is emerging. The mean age (59 years), gender (65% male), and COVID-19 features appeared to reflect those of hospitalized COVID-19 patients early in the pandemic. The mean time from COVID-19 symptoms to GBS symptoms was 11 days. The clinical presentation and severity of these GBS cases was similar to those with non–COVID-19 GBS. The EDx pattern was considered demyelinating in approximately half of the cases. Cerebrospinal fluid, when assessed, demonstrated albuminocytologic dissociation in 76% of patients and was negative for severe acute respiratory distress syndrome–coronavirus-2 (SARS-CoV-2) in all cases. Serum antiganglioside antibodies were absent in 15 of 17 patients tested. Most patients were treated with a single course of intravenous immunoglobulin, and improvement was noted within 8 weeks in most cases. GBS-associated COVID-19 appears to be an uncommon condition with similar clinical and EDx patterns to GBS before the pandemic. Future studies should compare patients with COVID-19–associated GBS to those with contemporaneous non–COVID-19 GBS and determine whether the incidence of GBS is elevated in those with COVID-19.     

Turns-amplitude analysis of the electromyographic recruitment pattern disregarding force measurement. II. Findings in patients with neuromuscular disorders.

Turns-amplitude analysis of the electromyographic recruitment pattern was performed on-line in the brachial biceps muscle of 46 patients with neuromuscular disorders using the mean amplitude as an indicator of force. The parameters, peak-ratio (PR) and number of time intervals (TI) from 0 to 1.5 ms, were increased in patients with myopathy. In patients with neurogenic involvement, the characteristic pattern was a decreased PR and a decreased incidence of TI between 0 and 1.5 ms. The results indicate that the two parameters supplement each other as some of the patients were identified only by one or the other. In patients with myopathy, the method had a higher diagnostic yield than the individual motor unit action potential analysis. The method is objective, fast, and reliable.     

Turns analysis (peak ratio) in EMG using the mean amplitude as a substitute of force measurement

Several modifications of turns and mean amplitude analysis of the electrical activity of muscles have been suggested to avoid measurement of force. Previously we have shown that these modifications may overlook abnormalities if the analysis is performed at high force. A new modification is suggested: analysing the maximal value of the ratio of turns to mean amplitude (peak ratio), using the mean amplitude as an indication of force. It has been applied to a material in which the force was measured. The peak ratio was increased in 82% of 17 patients with myopathy and decreased in 36% of 14 patients with neurogenic disorders.     

The human tongue during sleep: electromyographic activity of the genioglossus muscle.

The bilateral genioglossi muscles of the tongue, by virtue of their protrusive action, play an important role in the mechanics of maintaining an open air passage in the oropharyngeal region. Electromyographic studies of the genioglossus muscle during sleep were conducted in six healthy human volunteers. The principal results are the following: In quiet waking and in quiet sleep (stages I–IV), the genioglossal EMG is characterized by continuous discharges with substantially augmented activity during inspiration. When the subject enters REM sleep, the tonic genioglossal activity almost ceases except for small bursts during the inspiratory phase of respiration. During REM sleep, genioglossal motor units may become inactive for periods of up to 90 sec. However, these silent periods are regularly interrupted by three to six vigorous inspiratory genioglossal bursts. Genioglossal EMG with substantially augmented discharges during inspiration are best recorded from indwelling electrodes placed close to the origin of the muscle. During snoring, the genioglossal activity related to certain phases of the respiratory cycle is vastly increased. The results are discussed in context with problems encountered in hypersomniac patients with the sleep apnea syndrome.