零交导数与多波长系数分光光度法测定复方阿司匹林中3组分的含量

用零交导数及多波长系数分光光度法测定了复方阿司匹林片中阿司匹林、扑热息痛、咖啡因的含量。２种方法简单、快速且均不需分离样品。多波长系数法３组分回收率分别为９９．８１％，１００．３８％，９９．８７％。ＲＳＤ分别为１．０％，０．３％，０．９％。零交导数法３组分回收率分别为９９．１８％，１００．８１％，９８．８７％，ＲＳＤ分别为１．１％，０．８％，１．４％。     

白马足药液与正红花油对照治疗跟痛症109例

用白马足药液治疗跟痛症５７例（治愈率７３．７％．显效率１４％，有效率１０．５％，无效率１．８％，总有效为９８．２％），与正红花油治疗５２例（治愈率２１．２％，显效率３４．６％，有效率３６．５％，无效率７．７，总有效率为９２．３％）对照，经统计学的处理，两组总有效率无显著差异，而治愈率有显著差异。     

薄层扫描法测定麻黄中麻黄碱和2，3，5，6—四甲基吡嗪的含量

用薄层扫描法测得草麻黄和木贼麻黄中麻黄碱的含量分别为0．480％和0．560％，变异系数为1．80％和0．932％，测得二者中2，3，5，6－四甲基吡嗪的含量分别为0．0624％和0．0720％，变异系数为1．73％和1．00％，麻黄碱和2，3，5，6－四甲基吡嗪的回收率分别为98．1％和97．3％。     

零交导数与多波长系数分光光度法测定复方阿司匹林中3组分的含量

 用零交导数及多波长系数分光光度法测定了复方阿司匹林片中阿司匹林、扑热息痛、咖啡因的含量。２种方法简单、快速且均不需分离样品。多波长系数法３组分回收率分别为９９．８１％，１００．３８％，９９．８７％。ＲＳＤ分别为１．０％，０．３％，０．９％。零交导数法３组分回收率分别为９９．１８％，１００．８１％，９８．８７％，ＲＳＤ分别为１．１％，０．８％，１．４％。     

剖宫产452例因素分析

目的探讨剖宫产率增高的因素。方法对2003年1月-2006年12月452例剖宫产病例进行回顾性分析。结果剖宫产率平均为44．41％，主要原因依次为相对头盆不称（枕横位、枕后位）（11．28％），臀位（8．62％），羊水过少（9．95％），胎儿窘迫（7．52％），脐带绕颈（7．07％），前置胎盘（0．66％），过期妊娠（4．64％），高龄初产（2．65％），骨盆狭窄（7．96％），瘢痕子宫（9．95％），妊高征（3．98％），巨大儿（4．64％），双胎（1．99％），社会因素（19．03％）。结论提高产科医生的技术水平，加强孕产期监护，医患配合的条件下，合理掌握剖宫产指征，在一定程度上可降低剖宫产率。     

中西医结合治疗急性发作期肺心病65例

采用活血化瘀法，以桃红四物汤为基本方，配合西药抗感染，中西医结合治疗肺心病急性发作期患者６５例，并设对照组西药综合治疗５２例。结果：治疗组显效６１．５％，好转３２．３％，无效６．２％，总有效率为９３．８％；对照组显效４２．３％，好转３８．５％，无效１９．２％，总有效率为８０．８％。两组总有效率比较有显著差异（Ｐ＜０．０５）。     

羧基化氨基多糖对5种胃溃疡模型的影响

目的：观察羧基化氨基多糖药物抗胃溃疡的作用。方法：选用酒精型，阿斯匹林型，盐酸型，醋酸型实验性胃溃疡和大鼠束缚，水浴应激性胃溃疡模型进行实验。结果：对5种胃溃疡均有不同的抑制作用。大，中，小剂量组溃疡抑制率依次分别为：42．09％，35．55％，33．54％，53．65％，49．48％，46．76％，51．48％，44．09％，33．64％，49．44％，46．23％，45．10％和50．12％，44．63％，41．92％。结论：羟基化氨基多糖具有预防和治疗胃溃疡的作用。其效应与德诺，法莫替丁和甲氰米胍相近。     

胃炎消治疗胃癌癌前期病变疗效分析

运用胃炎消冲剂治疗７０例胃癌癌前期病变的患者，治疗组症状痊愈率３９．５３％，显效率为６７．４４％，总有效率为９０．７０％，对照组分别为１１．１１％，４０．７４％，７０．３７％，两组比较差别显著性意义，治疗组不典型增生和不完全性结肠型肠上皮化生痊愈率为２５．５８％，显效率为４４．１９％，总有效率为７２．０９％，对照组分别为３．７０％，１１．１１％，５１．８５％，两组比较差别有显著性意义，治疗组伴随的     

单相抑郁发作与双相抑郁发作中医证候学比较

目的：比较抑郁症不同表现类型一单相抑郁发作和双相抑郁发作中医证候特征，为中医辨证治疗不同类型抑郁症提供依据。方法：观察符合研究标准患者共459例。按照中医辨证的原理，结合郁证的特点，编制抑郁症中医证候流行病学调查表搜集四诊资料。结果：在459例情感障碍目前为抑郁发作的患者中，344例为单相抑郁占74．9％；115例为双相抑郁，占25．1％。单相抑郁组，男性109例占31．7％，女性235例占68．3％；双相抑郁组，男性55例占47．8％，女性60例占52．2％。单相抑郁组总病期为75．6±28．7月，双相抑郁组为139．9±33．7月。单相抑郁组有诱因者为242例，占70．3％；双相抑郁组为44例，占38．3％。单相抑郁组气虚197例占57．3％、火热185例占53．8％、阴虚138例占39．2％，血瘀193例占56．1％；双相抑郁组分别为84例占73．0％、26例占22．6％、16例占13．9％，血瘀52例占45．2％。单相抑郁组涉及中医病住肝186例占54．1％，脾196例占57．0％，双相抑郁组涉及肝99倒占86．1％，脾95例占82．6％。单相抑郁组肝郁气滞型12例占3.5％，肝郁脾虚型15倒占4．4％，痰气郁结型17倒占4．9％，气郁化火型79例占23．0％，脾虚湿盛型92例占26．7％，心脾两虚型8例占2．3％，脾肾两虚型87倒占25．3％，肝肾阴虚型34例占9．9％；双相抑郁组肝郁气滞型4例占3．5％，肝郁脾虚型38例占33．0％，痰气郁结型11倒占9．6％，气郁化火型9例占7．8％，脾虚湿盛型19例占16．5％，心脾两虚型11例占9．6％，脾肾两虚13倒占11．3％，肝肾阴虚型10例占8．7％。上述方面比较两组有显著性差异。结论：中医流行病学资料、证候学、病位和辨证方面两者有差异。     

褶合曲线分析法同时测定撒痛风注射液中3组分含量

 应用计算机辅助福合曲线分析法，结合计算机信，包处理技术，不经分离，同时测定了撒痛风注射液中水杨酸钠、安替比林、咖啡因３个组分的含量，结果满意，平均回收率和相对标准偏差分别为１００．４％，０．２３％；１００．３％，０．３４％，９９．８８％，０．４５％。     

温胆汤加味治疗梅尼埃病的临床观察

目的:观察理气健脾,清热化痰,和胃降逆类中药治疗梅尼埃病临床疗效。方法:治疗组36例,采用温胆汤加味治疗,设对照组36例,单用西药治疗。结果:治疗组:痊愈率50%,显效率28%,有效率19%,无效率2.8%,总有效率为97.2%。对照组:痊愈率25%,显效率27.7%,有效率33.3%,无效率13.9%,总有效率为86.1%。结论:温胆汤加味治疗梅尼埃病有显著疗效。     

����CTLA4-Fc�ںϵ��׵������о�

??OBJECTIVE To develop the quality control methods of a recombinant CTLA4-Fc fusion protein. METHODS Mehtods for quality research of CTLA4-Fc fusion protein were established, such as reporter gene assay for bioassay based on CTLA4/IL-2-Luciferace/Jurkat cells, binding activity with B7 by SPR, purity by SEC-HPLC and CE-SDS, charge heterogeneity analysis by IEF, identity by peptide mapping and MECK, determination of sialic acid by RP-HPLC and content analysis of N-glycan by HILIC-HPLC. RESULTS The bioactivity EC₅₀ of CTLA4-Fc fusion protein was (0.40??0.08) ??g??mL^-1, and the RSD was 20.00%. The relative binding activity with B7 by BIAcore test was (91.72??0.12)%,and the RSD was 0.14%. The purity determined by SEC-HPLC was(99.09??0.01)%,and the RSD was 0.01%. The purity determined by reduced CE-SDS was(100??0.00)%. The purity determined by non-reduced CE-SDS was(98.50??0.17)%, and the RSD was 0.18%. The pI of its major zone ranged from 4.6 to 5.9, which was consistent with the reference substance in peptide mapping. CTLA4-Fc fusion protein and its variant could be identified by MECK. The content of sialic acid determined by RP-HPLC was (10.16??0.41)mol/mol protein and the RSD was 4.07%. The contents of major N-glycoforms were as follows:G0F was 7.5%,G1F was 15.0%,G2F was 17.1%,G1FS1 was 4.2%,G2FS1 was 19.5% and G2FS2 was 9.6%. CONCLUSION The evaluation methods for critical quality attribute of CTLA4-Fc fusion protein were been initially established and provide reference for the quality control of this product.     

复合酶法提取人参总皂苷

目的在人参根及茎叶总皂苷提取工艺中引入酶因素,提高人参总皂苷提取率。方法以人参总皂苷提取率为指标,运用单因素实验及正交试验确定复合酶作用的最佳工艺。结果无酶条件下人参根中总皂苷的提取率为3.22%,复合酶辅助法提取人参根总皂苷的最佳工艺条件为:中温淀粉酶5%,中性蛋白酶1%,时间2 h,温度60℃,得提取率为5.44%;无酶条件下人参茎叶中总皂苷的提取率为5.85%,复合酶辅助法提取人参茎叶总皂苷的最佳工艺条件为:漆酶5%,纤维素酶5%,甘露聚糖酶1%,时间2 h,温度40℃,提取率为11.93%。结论复合酶辅助提取法能够显著提高人参总皂苷提取率,且工艺简单、稳定性好,可用于产业化生产。     

Effect of an antidiabetic polysaccharide from Inula japonica on constipation in normal and two models of experimental constipated mice

OBJECTIVES: The aim of the present study was to investigate the effect of an antidiabetic polysaccharide (IJP) from Inula japonica on gastrointestinal transit in normal mice and on constipation in two models of constipated mice. METHODS: Two models of constipation in mice were respectively induced by fasted water for 4 days or induced by diphenoxylate. The normal and constipated mice were administered IJP once at doses of 100 and 400 mg/kg (p.o.), the gastrointestinal vermicular motion, start time of defecation, number and weight of stool were investigated. RESULTS: After administration of IJP, the gastrointestinal propulsive rate was increased by 9.79% and 10.42%, the start time of defecation was shortened by 37.27% and 44.06%, the number of feces increased by 115.4% and 130.8% in normal mice. In fasting‐water constipated mice, the start time of defecation was shortened by 9.69% and 30.52% by IJP, defecation granules raised by 22.09% and 39.53%, wet feces weights were increased by 23.50% and 39.14% compared with the untreated constipated mice. In diphenoxylate‐induced mice, the start time of defecation was shortened by 25.48% and 28.13%, defecation granules raised by 100.0% and 118.0%. CONCLUSIONS: Consumption of IJP effectively improved bowel movement, stool output observed in this study. IJP may be practical in relieving constipation in the elderly diabetic population. Copyright © 2010 John Wiley & Sons, Ltd.     

中西医结合治疗幽门螺杆菌相关性胃炎60例疗效观察

采用中西医结合方法治疗幽门螺杆菌相关性胃炎６０例，并与单纯中药治疗５８例及丽珠得乐治疗６０例对照。结果：治疗组中显效４３例（占７１．６％），好转１６例（占２６．７％），无效１例（占１．７％），总有效率为９８．３％；中药组中显效２１例（占３６．２％），好转２４例（占４１．４％），无效１３例（占２２．４％），总有效率为７７．６％；得乐组中显效２３例（占３８．３％），好转２５例（占４１．７％），无效１２例（占２０．０％），总有效率为８０．０％。经统计学处理，３组显效率及总有效率对比，治疗组与对照组间有显著差异（Ｐ＜０．０１）。     

不同精制工艺对金银花水提液中绿原酸含量的影响

目的：考察不同工艺精制金银花水提液的效果,优选出金银花的最佳精制工艺。方法：采用醇沉、离心-超滤、微滤-超滤、大孔吸附树脂等4种工艺对金银花水提液进行精制,并采用HPLC法测定金银花水提液中绿原酸的含量,对4种工艺进行了定量对比研究。并对4种工艺操作过程进行了比较。结果：金银花水提液经醇沉、离心-超滤、微滤-超滤、大孔吸附树脂工艺处理后,其绿原酸的转移率分别为59.30%、83.07%、80.13%、23.47%;固形物去除率分别为36.59%、40.12%、39.81%、90.07%。结论：离心-超滤工艺精制后绿原酸的转移率高于其他方法,固形物去除率适中,具有简化工艺,缩短操作周期的优点,可作为金银花水提液的最佳精制工艺。     

HPLC同时测定通脉口服液中5种有效成分的含量

 目的 建立通脉口服液中5种活性成分的含量测定方法。方法 采用HPLC, 流动相：A相为甲醇,B相为0.1%冰醋酸;流速：1.0 mL·min-1 ;检测波长：280,320 nm。结果 咖啡酸、葛根素、阿魏酸、隐丹参酮、丹参酮ⅡA均达到较好的分离,峰型良好,线性关系良好,平均加样回收率分别为100.13%,100.70%,99.67%,100.38%,100.77%;RSD分别为1.97%,1.33%,2.18%,2.24%,1.23%。结论 该方法操作简单,准确,重复性好。     

僵蚕提取液中蛋白质和草酸铵等成分的定量分析

目的对僵蚕提取液中三种形态的含氮物质、草酸盐、总离子浓度和还原性物质进行含量测定。方法采用离子交换和经典化学分析方法。结果僵蚕提取液中固形物含量为38.52%,RSD=0.92%(n=3);总氮、铵态氮和酰胺态氮含量分别为5.798%、0.868%、0.196%,蛋白质含量为29.74%,占总固形物的77.2%;草酸铵含量为3.85%(不包括0.96%的草酸根对应的其他盐),占总固形物的9.98%;总离子浓度按一价离子计为1.099mol/L;能被高锰酸钾在酸性条件下氧化的还原性物质含量相当于1.015mol/L的草酸盐浓度,其中阳离子型、阴离子型和非离子型还原物质浓度占总还原物质的摩尔百分率分别为7.84%、64.16%和不低于28.0%。结论本研究为进一步研究僵蚕提取液的抗凝作用和药理作用机理奠定了一定的物质基础。     

产地加工和炮制对蟾酥药材及饮片质量的影响

目的:评价不同干燥和炮制方法对蟾酥药材及饮片质量的影响.方法:每20 g鲜蟾酥分别采用下列干燥方法处理:105℃烘干,80℃烘干,60℃烘干,60℃减压干燥,冷冻干燥;同一干蟾酥药材采用酒制和乳制两种炮制方法制备相应饮片;HPLC法测定于蟾酥及其炮制品中5种吲哚生物碱和5种蟾毒配基的含量.结果:不同干燥方法得到的蟾酥5种生物碱总含量依次为17.57％ ±0.15％,20.01％ ±0.45％,19.99％ ±0.68％,19.85％ ±0.25％,20.12％ ±0.27％;5种蟾毒配基的总含量依次为19.91％ ±0.17％,20.20％ ±0.17％,19.96％ ±0.06％,20.24％ ±0.17％,21.05％ ±0.13％.炮制前、酒制和乳制后蟾酥中吲哚生物碱总含量分别为15.62％ ±0.29％,15.77％ ±0.24％,15.78％ ±0.27％;蟾毒配基总含量分别为20.69％±0.17％,20.74％±0.09％,22.12％ ±0.21％.结论:根据成分含量和样品外观色泽,冷冻干燥是最优的干燥方式;但综合考虑产地加工的相关因素(时间、成本和操作便利性等),60～80℃烘干也是一种经济实用的选择;酒制和乳制对蟾酥中5种吲哚生物碱和5种蟾毒配基类成分的含量基本无影响.     

Quaternary benzo[c]phenanthridine alkaloids as inhibitors of aminopeptidase N and dipeptidyl peptidase IV.

Chelerythrine, sanguinarine and an alkaloid extract from Macleaya cordata--sanguiritrin--were found to be inhibitors of aminopeptidase A and dipeptidyl peptidase IV, while fagaronine inhibited dipeptidyl peptidase IV only. At 50 microM, chelerythrine, sanguinarine and sanguiritrin inhibited aminopeptidase N by 82%, 82%, 88%, DPP IV by 38%, 62%, 57%, and fagaronine by 34%, respectively. When bovine serum albumin (500 microg/mL) was added, the inhibition of both proteases by quaternary benzo[c]phenanthridine alkaloids (QBA) (50 microM) was significantly diminished. Strong interaction of chelerythrine and sanguinarine with bovine and human serum albumin was proved by electrophoretic determination of their respective conditional binding constants.