Cereal bran: the next super food with significant antioxidant and anticancer potential

Bran is the hard outer layer of cereal grains, rich in a myriad of healthy phytochemicals viz. phenolics, flavonoids, glucans and pigments. Unfortunately, these nutrition-rich components are often discarded during milling out of ignorance, organoleptic reasons and rancidity problems. Knowing the phytochemical constituents and pharmacological profile of bran is expected to give insight to their potential application in promotion of health. This study illustrates the antioxidant and anticancer properties of the cereal bran and its derivatives. The current contribution provides a comprehensive review of the bioactive phytochemical constituents of bran, their extraction techniques, the antioxidant and anticancer properties. The issues associated with bran stabilization in foods and enhancement strategies for extraction and bioavailability of bran nutrients are discussed. Bacteria and yeast fermentation of bran have been observed to improve their nutritional profile. Bran products promise potential applications as nutritional ingredients in functional foods. Leading food manufactures have already started fortifying food products with bran, at the wake of the discovery of its overwhelming health-promoting potentials.     

In vitro activity of dietary flavonol congeners against human cancer cell lines

Background  

Flavonoids have physiological activity and a variety of pharmacological properties, including anticancer activity in vitro, but structure–anticancer activity relationships are unclear.     

Exploiting biological activities of brown seaweed Ecklonia cava for potential industrial applications: a review

Seaweeds are rich in vitamins, minerals, dietary fibres, proteins, polysaccharides and various functional polyphenols. Many researchers have focused on brown algae as a potential source of bioactive materials in the past few decades. Ecklonia cava is a brown seaweed that is abundant in the subtidal regions of Jeju Island in the Republic of Korea. This seaweed attracted extensive interest due to its multiple biological activities. E. cava has been identified as a potential producer of wide spectrum of natural substances such as carotenoids, fucoidans and phlorotannins showing different biological activities in vital industrial applications including pharmaceutical, nutraceutical, cosmeceutical and functional food. This review focuses on biological activities of the brown seaweed E. cava based on latest research results, including antioxidant, anticoagulative, antimicrobial, antihuman immunodeficiency virus, anti-inflammatory, immunomodulatory, antimutagenic, antitumour and anticancer effects. The facts summarized here may provide novel insights into the functions of E. cava and its derivatives and potentially enable their use as functional ingredients in potential industrial applications.     

The nutritional and health benefits of mushrooms

Both wild and cultivated mushrooms have been consumed by humans for their nutritional and medicinal benefits. Nutritionally, mushrooms are low in energy and fat but high in protein, carbohydrate, and dietary fibre. Mushrooms contain a variety of minerals and trace elements such as potassium, and copper and vitamins such as riboflavin, niacin, and folates. They have been used as food for centuries because of their unique taste. Apart from being recognised as a nutritious food, certain mushrooms are also an important source of biologically active compounds with potential additional medicinal value in Chinese medicine. Bioactive secondary metabolites found in mushrooms include phenolic compounds, sterols and triterpenes. In vitro and in vivo studies with mushrooms and isolated bioactive constituents have purported many pharmacological effects such as anti‐tumour, antioxidant, antiviral, hypocholesterolemic and hypoglycaemic effects. Consumption of mushrooms or mushroom products in our daily diet may provide health benefits.     

Fucoidan from the sporophyll of Undaria pinnatifida suppresses adipocyte differentiation by inhibition of inflammation-related cytokines in 3T3-L1 cells

Obesity is a metabolic disorder, associated with cardiovascular disease and type 2 diabetes mellitus. Recent studies suggest that seaweed extracts are a significant source of bioactive compounds that are similar to dietary phytochemicals. Fucoidan, which is extracted from brown seaweeds, has a number of physiological functions. However, it is still unclear whether fucoidan would be beneficial in adipogenesis. In this study, we hypothesized that fucoidan extracted from the sporophyll of U pinnatifida exerts anti-obesity effects via inhibition of inflammatory-related cytokines. Thus, to test our hypothesis, we determined the obesity-specific therapeutic action of fucoidan in 3T3-L1 adipocytes. Herein, we showed that proliferator-activated receptor γ, CCAAR/enhancer-binding protein α, and adipocyte protein 2 were significantly suppressed in the presence of fucoidan, which decreased expression of the inflammation-related genes during adipogenesis in 3T3-L1 adipocytes. Moreover, fucoidan also reduced the accumulation of lipids and reactive oxygen species production in adipocytes. In conclusion, these results demonstrate that fucoidan from the sporophyll of U pinnatifida suppresses adipogenesis through the inhibition of major markers and inflammation-related cytokines in adipocytes. Hence, these findings indicate that fucoidan may afford some potential to control or reduce obesity.     

Chemical composition,nutritional and antioxidant properties of the red edible seaweed Porphyra columbina

Proximate composition, fatty acids and amino acid profiles and nutritional (chemical score, protein digestibility, PDCAAS and mineral dialyzability) and antioxidant properties (TEAC, DPPH and power reduction) from Porphyra columbina were evaluated. Total dietary fiber (48.02?±?1.13?g/100?g dry weight) and protein (24.61?±?0.21?g/100?g dry weight) were the two most abundant components in this seaweed. The main saturated and unsaturated fatty acids were C16:0 and C20:5 (n?3), respectively. The limiting amino acid was tryptophan with a chemical score of 57%. Protein digestibility was 74.33?±?3.0%. Porphyra columbina has high mineral content with good Na/K relationship and medium value of potential mineral accessibility (P, Ca and Zn dializability: 18.75?±?0.01, 17.62?±?0.16 and 16.70?±?0.44, respectively). The highest antioxidant properties were obtained with an acetone/water extraction system. This work provides important information about chemical composition and nutraceutical new properties of P. columbina.     

Diet Therapy for Cancer Prevention and Treatment Based on Traditional Persian Medicine

Cancer is the second leading cause of death with profound socio-economic consequences worldwide. Growing evidence suggests the crucial role of diet on cancer prevention and treatment. In Traditional Persian Medicine (TPM) there is a major focus on contribution of special diet and foods to cancer management. In the present article, the cytotoxic and antitumor activities of several food items including plants and animal products recommended by TPM as anticancer agents are discussed. Strong evidence supports the anticancer effects of beetroot (Beta vulgris) and its major compound betanin, cinnamon and cinnamaldehyde, barley (H. vulgare) and its products, extra-virgin olive oil, black pepper (P. nigrum) and its piperine, grapes (V. vinifera) and its compound resveratrol, ginger and its compound 6-gingerol, whey protein, fish, and honey. However, additional pharmacological studies and clinical trials are needed to elucidate their molecular and cellular mechanisms of actions, frequency, and amount of consumption, possible adverse effects, and optimum preparation methods. Moreover, studying mechanisms of actions of the bioactive compounds present in the discussed food items can be helpful in identifying and development of new anticancer agents.     

Antimutagenic and Anticancer Effects of Lactic Acid Bacteria Isolated From Tarhana Through Ames Test and Phylogenetic Analysis By 16S rDNA

Deaths due to cancer have been increasing in many countries in recent years. One of the beneficial probiotic properties is potential of antimutagenic and anticancer effects. The aim of this study was to investigate antimutagenic and anticancer effects of 25 strains of lactobacilli spp isolated from Tarhana in vitro conditions and to identify selected strains based on molecular biology. Antimutagenicity and anticarcinogenicity tests were performed based on proposed method of Professor Ames et al. Identification of selected strains was performed based on biochemical and molecular tests. Study of antimutagenic and anticancer effects of 25 different strains led to identification of 4 isolates of Lactobacillus spp. Higher antimutagenic activities against sodium azide were observed in cell suspensions of 4 strains as compared with their supernatants. So the inhibitory percentage of cell suspensions among 4 strains was equal to 60.38% in its highest level. Antimutagenicity of these strains was also increased in the presence of rat S9. Four selected strains were identified based on 16S rDNA sequence. Lactobacillus casei, Lactobacillus plantarum, and Lactobacillus brevis isolated from Tarhana exhibited high antimutagenic and anticancer activity. Of 25 strains of lactobacilli, 4 strains with the highest antimutagenicity effect were chosen as new potential probiotic strains.     

Curcumin and Melanoma: From Chemistry to Medicine

Melanoma is the most deadly form of skin cancer, with about 48,000 deaths each year worldwide. Growing evidence suggests that individual nutrients or dietary patterns might have important roles in the prevention of melanoma. Considering that melanoma is a potentially life-threatening cancer, novel protective and adjuvant treatments are needed to improve its prognosis. Curcumin is a bioactive substance extracted from rhizome of Curcuma longa L. Its global market is expected to grow in the next few years, especially in the pharmaceutical industry, due to its numerous physiological and pharmacological properties. For this review, we collected the available data on the protective and therapeutic role of curcumin against melanoma. We also discuss the chemistry, dietary sources, bioavailability, and metabolism of curcumin, and the mechanisms of action of its potential anticancer effects at the molecular level. Current challenges and future directions for research are also critically discussed.     

Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KBCD133+cells by downregulation of β-catenin

Abstract

Although, oral cancer therapies have been developed for decades, patient survival rates have not changed. Side effects of chemotherapy and radiotherapy reduce quality of life of patients and it remains difficult to treat oral cancers due to the presence of cancer stem cells (CSCs) that cause recurrence and metastasis. Therefore, we search for natural products that affect oral cancer cells including oral cancer stem cells. In the present study, we investigated the anticancer effects of Raphanus sativus L. seed (RSLS) extracts on oral squamous cell carcinoma KB cells and CSC-like KB^CD133+ cells. CD133 plays an important role in CSCs and physically binds to β-catenin to activate the β-catenin signaling targets. Therefore, a natural extract that can inhibit β-catenin act in may be effective anticancer drug acquiring CSC. Of the natural product extract candidates, RSLS extracts induced apoptosis in KB and KB^CD133+ cells and inhibited nuclear translocation of β-catenin cell migration and invasion rates. Treatment of RSLS extracts resulted in increases of Axin and it leds to reductions of β-catenin in KB and KB^CD133+ cells. Hence, the result suggests that RSLS are potential candidate for anticancer drug against oral cancer cells and CSCs.

Abbreviations CSC cancer stem cells

OSCC squamous cell carcinoma cells

RSLS Raphanus sativus L. seed.

     

Piperine Triggers Apoptosis of Human Oral Squamous Carcinoma Through Cell Cycle Arrest and Mitochondrial Oxidative Stress

Piperine is a nitrogenous pungent substance exhibiting multifunctional pharmacological properties. However, the mechanism underlying its anticancer potential is not well elucidated in human oral squamous carcinoma (KB) cell line. The anticancer potential of piperine was evaluated through potent biomarkers viz. reactive oxygen species (ROS), cellular apoptosis, and loss of mitochondrial membrane potential (MMP). In addition, cell cycle kinetics and caspases-3 activity were also carried out to confirm anticancer activity of piperine. Results showed that various concentrations (25–300 μM) of piperine exposure reduced the cell viability of KB cells significantly (P < 0.01). Piperine induced significant (P < 0.01) dose-related increment in ROS production and nuclear condensation. Moreover, piperine stimulated cell death by inducing loss of MMP, and caspase-3 activation. Cell cycle study revealed that piperine arrested the cells in G2/M phase and decreased the DNA content. Findings of this study suggest the efficacy of piperine in inducing cell death via the decrease in MMP and ROS liberation followed by caspase-3 activation and cell cycle arrest. Further assessment of the anticancer potency of piperine is needed for anticancer drug development.     

Anticarcinogenic Effects of the Ethanolic Extract of Salix aegyptiaca in Colon Cancer Cells: Involvement of Akt/PKB and MAPK Pathways

The bark from Salix species of plants has been traditionally consumed for its antiinflammatory properties. Because inflammation frequently accompanies the progress of colorectal cancer (CRC), we have evaluated the anticancer properties of the ethanolic extract from the bark (EEB) of S. aegyptiaca, a Salix species endogenous to the Middle East, using HCT-116 and HT29 CRC cell lines. Fresh bark from S. aegyptiaca was extracted with ethanol, fractionated by solvent-solvent partitioning and the fractions were analyzed by tandem mass spectrometry. Catechin, catechol, and salicin were the most abundant constituents of the extract. Interestingly, EEB showed the highest anticancer effect in the colon cancer cells followed by its fractions in ethyl acetate and water, with catechin, catechol, and salicin showing the least efficacy. EEB could strongly reduce the proliferation of the cancer cells, but not of CCD-18Co, normal colon fibroblast cell line. Accompanying this was cell cycle arrest at G1/S independent of DNA damage in the cancer cells, induction of apoptosis through a p53 dependent pathway and an inhibition of PI3K/Akt and MAP Kinase pathways at levels comparable to known commercial inhibitors. We propose that the combination of the polyphenols and flavonoids in EEB contributes toward its potent anticarcinogenic effects.

[Supplementary materials are available for this article. Go to the publisher's online edition of Nutrition and Cancer for the following free supplemental resource(s): Supplementary Figure 1 and Supplementary Figure 2.]     

Angio-Suppressive Effect of Partially Purified Lectin-like Protein from Musa acuminata pseudostem by Inhibition of VEGF-Mediated Neovascularization and Induces Apoptosis Both In Vitro and In Vivo

Lifestyle and nutritional changes have contributed much to the somatic genetic changes which have concurrently led to an increase cancer in humans. Hence the plant-based and nutritional involvements block oncogenic transformation are in good demand. We evaluate Phloem exudates of the dietary plant, Musa acuminate pseudostem, the initial domesticated plant species with the effective lectin activity for its functional role against the tumor development and its mechanism of action. Our experimental data exhibit that Musa acuminata Lectin Protein (MALP) shows a promising cytotoxic effect against the various human cancer cell lines. Supporting this, we evaluate the in vivo anti-tumor and anti-angiogenic activity of MALP in Ehrlich Ascites Carcinoma mice model (EAC). MALP treatment resulted in tumor growth inhibition and increased the lifespan of the EAC-bearing mice without showing any side effects on normal mice, as revealed by histological parameters. Further, a significant decrease in the ascites vascular endothelial growth factor (VEGF) secretion and microvessel density supports the anti-angiogenic property of the MALP. Apoptosis-inducing activity of MALP was revealed by DNA fragmentation assay, Caspase-3 inhibitor assay and cellular morphology were studied by fluorescence staining methods. Our study delivers the real evidence that MALP with a promising an anticancer potential expressively degenerates the tumor development by affecting angiogenesis and apoptosis.     

A New Protein Extract Inhibitor from Hypobranchial Purple Gland of Hexaplex trunculus,a Mediterranean Mollusk,Impairs the Motility of Human Glioblastoma U87 and the HeLa Cell Line of Cervical Carcinoma Cells

The aim of this study is to evaluate the effect of hypobranchial gland protein extracts (HGPEs) of Hexaplex trunculus on the viability, cell adhesion, and migration of human U87 glioblastoma cells and the HeLa cell line obtained from epithelial cervical carcinoma cells. Analysis of the HGPE on polyacrylamide gel (12%) shows a variety of proteins whose molecular weights vary between 12 and 1OO kDa. Chromatographic analysis shows 16 peaks obtained at various retention times. Cytotoxic effect was observed after 24 hours of incubation at the concentrations 20, 40, and 60 μg/ml in a dose-dependent manner. Concentrations giving 50% inhibition (IC₅₀) are 22 μg/ml for U87 and 15 μg/ml for HeLa cells. Our results show inhibition of U87 and HeLa cancer cell adhesion at concentrations of 10 and 20 µg/ml, respectively. High-pressure liquid chromatography fractions did not show antiadhesive effect on both cancer cell lines. The presence of HGPEs completely blocked the migration of the two cancer cell lines at 10 µg/ml. This inhibition is dose-dependent. IC₅₀ is about 2.5 μg/ml for both cancer cells. The HGPE of Hexaplex trunculus may have the potential to serve as a model for future anticancer drug development with probably a synergistic activity of the proteins of this extract.     

Health Benefits,Pharmacological Effects,Molecular Mechanisms,and Therapeutic Potential of α-Bisabolol

α-Bisabolol is one of the important monocyclic sesquiterpenes, derived naturally from essential oils of many edible and ornamental plants. It was first obtained from Matricaria chamomilla, commonly known as chamomile or German chamomile. The available literature indicates that this plant along with other α-Bisabolol containing plants is popularly used in traditional medicine for potential health benefits and general wellbeing. Nutritional studies are indicative of the health benefits of α-Bisabolol. Numerous experimental studies demonstrated pharmacological properties of α-Bisabolol including anticancer, antinociceptive, neuroprotective, cardioprotective, and antimicrobial. This review aims to collectively present different pharmacological activities based on both in vitro and in vivo studies. In the present review using synoptic tables and figures, we comprehensively present that α-Bisabolol possesses therapeutic and protective activities, therefore, it can be used for potential health benefits based on pharmacological effects, underlying molecular mechanism, and favorable pharmaceutical properties. Based on the studies mostly performed on cell lines or animal models, it is evident that α-Bisabolol may be a promising nutraceutical and phytomedicine to target aberrant biological mechanisms which result in altered physiological processes and various ailments. Given the polypharmacological effects and pleiotropic properties, along with favorable pharmacokinetics, and dietary availability and safety, α-Bisabolol can be used as a dietary agent, nutraceutical or phytopharmaceutical agent or as an adjuvant with currently available modern medicines. The regulatory approval of this molecule for use as food additives, and in cosmetics and fragrance industry is also supportive of its human usage. Moreover, further studies are necessary to address pharmaceutical, pharmacological, and toxicological aspects before clinical or nutritional usage in humans. The biological actions and health benefits open opportunities for pharmaceutical development with pharmacological basis of its use in future therapeutics.     

The Chemical Composition and Health-Promoting Effects of the Grewia Species—A Systematic Review and Meta-Analysis

Globally grown and organoleptically appreciated Grewia species are known as sources of bioactive compounds that avert the risk of communicable and non-communicable diseases. Therefore, in recent years, the genus Grewia has attracted increasing scientific attention. This is the first systematic review which focusses primarily on the nutritional composition, phytochemical profile, pharmacological properties, and disease preventative role of Grewia species. The literature published from 1975 to 2021 was searched to retrieve relevant articles from databases such as Google Scholar, Scopus, PubMed, and Web of Science. Two independent reviewers carried out the screening, selection of articles, and data extraction. Of 815 references, 56 met our inclusion criteria. G. asiatica and G. optiva were the most frequently studied species. We found 167 chemical compounds from 12 Grewia species, allocated to 21 categories. Flavonoids represented 41.31% of the reported bioactive compounds, followed by protein and amino acids (10.7%), fats and fatty acids (9.58%), ash and minerals (6.58%), and non-flavonoid polyphenols (5.96%). Crude extracts, enriched with bioactive compounds, and isolated compounds from the Grewia species show antioxidant, anticancer, anti-inflammatory, antidiabetic, hepatoprotective/radioprotective, immunomodulatory, and sedative hypnotic potential. Moreover, antimicrobial properties, improvement in learning and memory deficits, and effectiveness against neurodegenerative ailments are also described within the reviewed article. Nowadays, the side effects of some synthetic drugs and therapies, and bottlenecks in the drug development pathway have directed the attention of researchers and pharmaceutical industries towards the development of new products that are safe, cost-effective, and readily available. However, the application of the Grewia species in pharmaceutical industries is still limited.     

A Study Of The Chemical And Nutritional Characteristics Of An Experimental Canadian Sweet Lupin Variety

The lupin, a leguminous plant, is a potential oilseed crop in regions withtemperature climates. This plant contains toxic constituents which appear to bequinolizidine alkaloids. Attempts to breed a sweet lupin variety with lowerlevels of these alkaloids have been reported. This paper examines the chemicaland nutritional properties of an experimental lupin grown in Manitoba, Canadacompared to a bitter lupin variety. The experimental lupin variety contained 27.2 percent protein and 14.8 percent fat compared to 39.0 percent proteinand 10.7 percent fat for the bitter lupin . Amino acid composition indicateda low level of me thionine for both lupin s, while fatty acid analysis showedsimilar patterns with CIS: 1 being the predominant fatty acid together withhigh levels of Cl8:2 and Cl8:3. The level of alkaloids was similar for bothlupins. However, the nutritional study found the experimental lupin dietcompared favorably with casein with respect to PER and was significantly(P<0.01) higher than the corresponding bitter lupin. Based on the higher PERvalue the experimental lupin variety compared favorably with earlier studieson sweet lupins. It was apparent, however, that qualitative differences in totalalkaloids must account for the high PER in weanling Wistar rats fed theex perim ental lupin. The qualitative differences between the experimentalsweet and bitter lupin varieties requires closer examination in order to explainthe superior nutritional quality of the experimental lupin variety.     

Cancer Chemopreventive Effects of the Flavonoid-Rich Fraction Isolated from Papaya Seeds

Intervention to decelerate, arrest, or reverse the process of carcinogenesis by the use of either natural or synthetic agents individually or in combination has emerged as a promising and pragmatic medical approach to reduce cancer risk. In the present study, we examined the cancer chemopreventive potential of a flavonoid-rich fraction isolated from the seeds of Carica papaya, a plant traditionally referred to as papaw. The flavonoid-enriched benzene fraction of the aqueous extract exerted its anticancer properties in vitro through cytoprotection, antioxidative and antiinflammatory mechanisms and genoprotection in response to isocyanate-induced carcinogenicity. Medium-term anticarcinogenicity and 2-stage skin papillomagenesis studies conducted in benzopyrene-induced lung carcinogenesis and 7,12-dimethyl benz(a)anthracene-mediated skin papillomagenesis mouse models further validated our in vitro observations. This is the first demonstration of chemopreventive activities of papaya seed products, however, further studies to understand the subtle targets of intracellular signaling pathways, pharmacological profile and toxicological safety of this bioactive fraction are essential to pave the way for successful clinical translation. Our study supports the inverse association between dietary flavonoid intake and cancer risk.     

Ethnobotanical and chemical surveys of an edible wild legume: Uraria Picta (Jacq.) DC.

Urariapicta (Jacq.) DC. belongs to the family Leguminosae: Papilionoidae. It is an annual woody erect herb or under‐shrub with edible seeds. In order to evaluate what is known about this species and its seed consumption in Ivory‐Coast, an ethnobotanical investigation was carried out in the department of Séguéla, in the north of the country. The contents of protein and lipid in seeds were analyzed.

The ethnobotanical surveys revealed that people belonging to the Malinké ethnic group no longer eat the seeds of Uraria picta. Furthermore, the local knowledge about the species is decreasing from generation to generation.

Nevertheless, chemical analyses show that the species is a good source of some nutrients. The protein characteristics of U. picta seeds were compared with several frequently cultivated legumes and cereals. The correlation coefficient calculated for essential amino acids show that U. picta seeds are very close to cultivated legumes (garden pea, horse bean, kidney bean and Bambara groundnut) and slightly close to cultivated cereals (bread wheat, rice, barley). In order to evaluate the nutritional value of protein, the chemical score was calculated. The value obtained, about 87.0%, indicated a great balance of essential amino acids. This value is higher than the chemical score calculated for some other cultivated crops, except Bambara groundnut. The results obtained were also compared with Indian material. This comparison revealed important differences about some amino acid contents. It appears that some variability's more or less important exist according to the region concerned.

The lipids content of the seeds is low (1.6%), but U. pida oil contains an interesting proportion of essential and long chain fatty acids.

It is concluded that, according to the potential nutritional value of its seeds, U. picta should be considered as a source of nutrients that may contribute in the diet of rural people.     

The Role of n-3 Polyunsaturated Fatty Acids in the Prevention and Treatment of Breast Cancer

Breast cancer (BC) is the most common cancer among women worldwide. Dietary fatty acids, especially n-3 polyunsaturated fatty acids (PUFA), are believed to play a role in reducing BC risk. Evidence has shown that fish consumption or intake of long-chain n-3 PUFA, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are beneficial for inhibiting mammary carcinogenesis. The evidence regarding α-linolenic acid (ALA), however, remains equivocal. It is essential to clarify the relation between ALA and cancer since ALA is the principal source of n-3 PUFA in the Western diet and the conversion of ALA to EPA and DHA is not efficient in humans. In addition, the specific anticancer roles of individual n-3 PUFA, alone, have not yet been identified. Therefore, the present review evaluates ALA, EPA and DHA consumed individually as well as in n-3 PUFA mixtures. Also, their role in the prevention of BC and potential anticancer mechanisms of action are examined. Overall, this review suggests that each n-3 PUFA has promising anticancer effects and warrants further research.