应用桡动脉鼻烟窝皮支皮瓣修复虎口挛缩

目的 探讨总结采用桡动脉鼻烟窝皮支皮瓣修复虎口挛缩的手术疗效.方法 采用桡动脉鼻烟窝皮支皮瓣修复虎口挛缩18例.本组病例虎口角平均20°,平均宽度19 mm.根据皮肤缺损情况在前臂桡侧设计皮瓣:以鼻烟窝中点为旋转点、前臂中立位时桡骨的走形线为轴线.结果 17例皮瓣全部成活,1例皮瓣部分坏死.全部病例均得以随访,随访时间5～26个月,平均12个月.虎口宽度增加45 mm,虎口角平均增加50°.结论 桡动脉鼻烟窝皮支皮瓣是一种简单、可靠的修复虎口挛缩的方法.     

前臂桡侧复合血管网皮瓣的临床应用

目的　报道前臂桡侧神经血管复合岛状皮瓣的解剖方法及临床应用效果。　方法　对手部创面及瘢痕挛缩以该皮瓣进行修复 2 2例 2 3处 ,切取皮瓣面积最大 13cm× 6cm ,最小 8cm× 4cm ,旋转点至皮瓣顶端最长 2 2cm ,旋转点设计在鼻烟窝或其近端 ,蒂部及皮瓣下筋膜中含有桡神经浅支、头静脉及桡动脉鼻烟窝上行支 ,含或不含前臂外侧皮神经。　结果　临床应用 2 2例 2 3块皮瓣 ,其中皮瓣全部成活 2 2块 ,部分坏死 1块。术后随访 1～ 3个月 ,成活的皮瓣质地优良 ,外观比较满意。　结论　前臂桡侧复合皮瓣的临床应用 ,皮瓣切取面积大 ,旋转点更远 ,可达手部较远端创面 ,给手部创面的修复提供了一种新选择。     

前臂带筋膜血管蒂皮瓣修复手部皮肤缺损

目的介绍采用前臂带筋膜血管蒂皮瓣修复手部皮肤缺损的方法。方法自2003年1月－2008年5月采用前臂带筋膜血管蒂皮瓣修复手部皮肤缺损27例,其中骨间背侧动脉皮瓣7例,尺动脉腕上支皮瓣11例,鼻烟窝皮瓣9例。结果25例皮瓣存活,2例皮瓣皮缘部分坏死,予换药后,创面愈合。术后随访6～18月,皮瓣质地及外观满意,患手功能恢复良好。结论前臂带蒂皮瓣是修复手部皮肤缺损的一种较好选择。     

桡动脉穿支蒂岛状皮瓣修复腕及前臂创面

目的 探讨桡动脉穿支蒂岛状皮瓣的解剖以及修复腕及前臂创面的手术方法和临床疗效.方法 以桡动脉搏动及体表投影为中心,采用多普勒血管仪探测桡动脉穿支的部位,以靠近缺损创面部的皮支作为血管蒂及旋转点,根据缺损创面大小、形状设计皮瓣,修复腕及前臂创面12例.结果 术后12例皮瓣中有2例出现静脉危象,经拆除部分缝线后存活,创面愈合,其余10例皮瓣均顺利存活.随访时间3～18个月,皮瓣质地软,外形及功能满意.结论 桡动脉穿支在桡骨茎突近端2.0cm、4.0～ 5.0 cm、7.0cm处较恒定,以此为蒂的岛状皮瓣血供可靠,操作简单,不损伤主干血管,修复后外形好,是修复腕及前臂皮肤软组织缺损的有效方法之一.     

应用桡动脉鼻烟窝穿支皮瓣修复手部皮肤缺损

目的 报道应用桡动脉鼻烟窝穿支皮瓣修复手部皮肤缺损创面.方法 应用桡动脉鼻烟窝穿支皮瓣对手部虎口、拇指等皮肤软组织缺损创面27例进行修复,皮瓣面积最大11.0 cm×6.5 cm,最小4.5 cm×2.0 cm,以腕部鼻烟窝中点为旋转点,轴线为桡骨茎突至小头的连线.结果 27例皮瓣均成活良好,无肿胀、淤血等现象,供区不能缝合的创面植皮均成活,随访6个月～2年,手部功能和外形良好.结论 鼻烟窝桡动脉穿支皮瓣血运好、质地柔软、切取方便,不牺牲主要动脉,是修复手部虎口、拇指创面的一种简单、安全、损伤小的方法.     

手背双逆行皮神经营养血管蒂皮瓣修复两手指背侧皮肤缺损

[目的]报道手背双逆行皮神经营养血管蒂皮瓣修复手指背侧皮肤缺损的方法。[方法]以桡、尺神经在手背侧的4个分支的营养血管为蒂设计各种皮瓣，同时应用其中的2种修复2个手指背侧的皮肤缺损。[结果]5例共10块皮瓣全部成活，伤指创面完全覆盖。[结论]逆行皮神经营养血管蒂皮瓣血供充分，不损伤知名血管，修复距离远，是修复手指背侧皮肤缺损的良好方法，可同时应用2个皮瓣以修复2个手指的皮肤缺损。     

桡动脉鼻烟窝穿支远端蒂岛状筋膜皮瓣修复手部烧伤七例

笔者单位收治烧伤患者 7例 ,其中男 5例、女 2例 ,年龄 2 9～ 5 7岁。致伤原因 :热压伤 3例 ,电击伤 4例。创面分布 :腕部 2例 ,虎口 1例 ,手掌 2例 ,手背 2例。创面面积为( 2cm× 3cm)～(7cm× 10cm),均有深部组织裸露。患者入院清创后 ,以鼻烟窝的中点为旋转点、桡骨的走行作为皮瓣的轴心线 ,向两侧设计皮瓣 ;按设计的皮瓣划线 ,分别切开皮瓣的桡、尺侧缘 ,由近向远紧贴深筋膜的表面 ,将皮瓣掀起至桡骨茎突处 ,保留血管蒂周围直径约 1.5cm的深筋膜组织 ;皮瓣游离完成后通过皮下隧道转移至受区。移植后 7例患者皮瓣全部成活 ,外观、功能良…     

前臂后外侧中段穿支皮瓣的临床应用

目的 探讨前臂后外侧中段穿支皮瓣的临床应用疗效.方法 根据解剖学基础,在前臂后外侧,沿肱骨外上髁至桡骨茎突的连线为皮瓣的轴心线设计前臂后外侧中段穿支皮瓣,转位修复前臂、腕部软组织缺损6例.结果 临床应用6例,术后皮瓣完全成活,皮瓣血供丰富,回流正常,无青紫、明显肿胀、水泡等,创面Ⅰ期愈合,经3～15个月随访,皮瓣色泽接近正常,质地和外形优良.结论 前臂后外侧中段穿支皮瓣是一血供稳定、丰富、带皮神经的非主干血管为蒂的皮瓣,可作为前臂非主干血管蒂皮瓣供区的新补充术式,供临床选用.     

胸脐皮瓣的临床应用

目的报道胸脐皮瓣四种术式的临床应用效果。方法应用游离胸脐皮瓣四种术式修复前臂、手、小腿、踝前及足背合并骨、肌腱、神经外露的创面151例。其中采用改良带蒂胸脐皮瓣修复前臂及手背创面19例，修复手脱套伤3例，游离胸脐皮瓣直接与受区血管吻合修复创面52例，游离胸脐皮瓣经携带桥式血管吻合修复创面36例，跨脐中线游离胸脐皮瓣修复创面41例。结果术后带蒂胸脐皮瓣全部成活；游离胸脐皮瓣直接与受区血管吻合有2例出现血管危象，经手术探查发现动脉栓塞1例，2例均行自体静脉移植后成活；游离胸脐皮瓣经携带桥式血管吻合的病例，皮瓣全部成活，无血管危象的发生；跨脐中线的超大胸脐皮瓣病例，1例远端皮缘坏死，经换药处理后痊愈。结论根据创面缺损大小选择胸脐皮瓣的四种术式修复均取得良好临床效果。     

尺神经手背支营养血管逆行筋膜皮瓣的临床应用

目的报道以尺神经手背支营养血管为蒂的逆行皮瓣修复手部皮肤软组织缺损的临床效果。方法根据应用解剖设计以掌指关节近侧为转轴点,沿走行于第4掌骨间隙的尺神经手背支营养血管轴为蒂、沿前臂尺侧下1/3至手掌尺背侧切取皮瓣逆行转位修复手腕部、手掌尺侧及环、小指皮肤软组织缺损创面。结果临床应用22例,皮瓣最大面积13cm×4．5cm,所有皮瓣全部成活。术后随访6～24个月,皮瓣质地优良,手部外形与功能恢复满意。结论该术式扩大了皮瓣的切取面积和修复范围。皮瓣切取快捷方便,转位灵活,不牺牲主要血管,血供可靠,是修复手及手指皮肤缺损的理想皮瓣。尤其适于修复手掌远端创面。     

杭州健康女性定量骨超声测定原发性骨质疏松

目的 评价杭州健康女性骨超声速度(SOS)值随增龄减少和骨质疏松患病率，建立杭州地区女性骨超声速度值参考数据库。方法 定量超声法测定1208例杭州地区健康女性桡骨远端(RAD)，第3指骨近节(PLX)，第V跖骨(MTR)和胫骨中段(TIB)的超声速度值。结果 RAD、PLX、MTR和TIBSOS峰值(Peak of SOS)均出现在40-45岁，TJB的SOS峰值出现在35—40岁，此后随年龄增长而下降。绝经后妇女在绝经后早期和晚期各有1个SOS快速减少期，前见于桡骨近端，平均年减少率为2．4％，后见于胫骨中段，平均年减少率为1．8％。各部位骨SOS累积减少率随年龄增长而增加，到85岁4部位累积减少为13％-18％。60岁以后骨质疏松性症(OP)检出率为45％-70％，OP检出率以桡骨远端最高，60-70岁平均为67％，第3指骨近端次之约50％，胫骨中段最低为36％；75岁以后分别为70％，65％和45％。结论 全身各部位骨超声速度值到达峰值的年龄不同，峰值也各有差异。绝经后妇女骨超声速度值随年龄增加减少较快，应予激素和补钙治疗，桡骨远端为本地区SOS检测和OP检出的敏感部位。     

Importance of echocardiography in prognosis of surgical outcomes in cholecystitis in elderly patients

The authors propose to use more often echocardiography (EchoCG) in examination of elderly (over 60 years) of age patients with cholecystitis that permits to increase surgical activity to 92.4%. Left ventricular ejection fraction is the most informative. When this fraction is lower than 45% surgery must be recommended on vital indications only. EchoCG was used in 155 patients with cholecystitis, 131 of them were operated. 2 (1.52%) patients died due to acute cardio-vascular insufficiency and pulmonary artery thromboembolism.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

目的 评价脊髓胶质细胞在小鼠骨癌痛形成中的作用.方法 健康雄性C3H/He小鼠40只,周龄8～10周,体重18～22 g,随机分为4组(n=10):假手术组(S组)、骨癌痛组(B组)、PBS组(P组)和米诺环素组(M组).S组跟骨骨髓腔内注射PBS 10 μl;余3组跟骨骨髓腔内注射含2×105个骨纤维肉瘤细胞的PBS 10 μl制备骨癌痛模型,于造模前即刻开始PBS组鞘内注射PBS 5μl,M组鞘内注射米诺环素(用PBS溶解为0.2 mmol/L)5μl,1次/d,连续11 d.于造模前1 d、造模后即刻、3、5、7、9、11 d时测定机械痛阈;于造模后3、7、9、11 d机械痛阈测定结束后测定冷痛阈.痛阈测定结束后处死小鼠,取脊髓组织,测定神经胶质纤维酸性蛋白(GFAP)和CD11b的表达水平.结果 与S组比较,B组和P组造模后3-11 d时、M组造模后3、5 d时机械痛阈升高,B组、P组和M组造模后7～11 d时冷痛阈升高,脊髓CD11b和GFAP表达上调(P<0.05).与B组比较,M组造模后3-11 d时机械痛阈降低,造模后7-11 d时冷痛阈降低,脊髓CD11b和GFAP表达下调(P<0.05).结论 脊髓胶质细胞(星形胶质细胞和小胶质细胞)的激活参与了小鼠骨癌痛的形成.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.