Survivin及Bak蛋白在人脑胶质瘤的表达及相关性研究

目的 探讨Survivin及Bak蛋白在人脑胶质瘤的表达,并分析二者的相关性.方法 采用免疫组织化学链霉素抗生物素蛋白-过氧化物酶连接法(SP法),检测Survivin及Bak蛋白在80例人脑胶质瘤组织中的表达,与10例正常脑组织进行对照.结果 Survivin及Bak蛋白在人脑胶质瘤组织阳性表达率分别为62.50%、63.75%,明显高于正常脑组织阳性表达率(P＜0.05),其中在Ⅰ和Ⅱ、Ⅱ和Ⅲ、Ⅲ和Ⅳ级胶质瘤间表达无显著性差异(P＞0.05),在Ⅰ、Ⅱ级和Ⅲ、Ⅳ级之间阳性表达率有显著性差异(P＜0.05),Survivin及Bak蛋白在人脑胶质瘤组织中的阳性表达呈负相关(r=-0.4303,P＜0.05).结论 Survivin及Bak蛋白在人脑胶质瘤的发生发展中起拮抗作用,二者可以作为肿瘤相关抗原来诊断和治疗胶质瘤.     

人脑星形细胞瘤组织中Survivin与P53的表达

目的:探讨人脑星形细胞瘤组织中Survivin及P53的表达情况.方法:采用免疫组织化学SP法检测65例脑星形细胞瘤(Ⅰ级18例,Ⅱ级20例,Ⅲ～Ⅳ级27例)及10例正常脑组织标本中Survivin、P53的表达并分析2者之间的关系.结果:正常及脑星形细胞瘤组织中Survivin的阳性表达率为0%和49.2%(32/65)(P＜0.01).星形细胞瘤Ⅰ级、Ⅱ级和Ⅲ～Ⅳ级组织中,Survivin阳性表达率分别为11.1%(2/18)、40.0%(8/20)、81.5%(22/27)(P=0.000).正常和脑星形细胞瘤组织中P53阳性表达率为0%和41.5%(27/65)(P＜0.05).星形细胞瘤Ⅰ级、Ⅱ级和Ⅲ～Ⅳ级组织中,P53的阳性表达率为0%、45.0%(9/20)、66.7%(18/27)(P=0.000).星形细胞瘤组织中Survivin与P53表达呈正相关(rs=0.481,P＜0.01).结论:survivin的激活与p53的突变在脑星形细胞瘤的发生发展中可能起协同作用.     

P21和survivin蛋白在脑胶质瘤组织中的表达及其临床意义

目的:探讨不同级别人脑胶质瘤组织和正常脑组织中P21和survivin蛋白的表达情况,分析其临床意义。方法:按2007年WHO制定的病理分级标准,将41份脑胶质瘤组织标本分为Ⅰ级9份、Ⅱ级14份、Ⅲ级10份和Ⅳ级8份,另外12份正常脑组织作为对照组,采用免疫组织化学法检测脑胶质瘤和正常脑组织中P21和survivin蛋白的表达情况,并分析两者表达的关系。结果:在正常脑组织和脑胶质瘤组织中P21及survivin蛋白阳性表达率分别为91.7%和53.7%及0%和51.2%,不同组织中P21和survivin蛋白的阳性表达率比较差异均有统计学意义(P<0.05)。Ⅰ-Ⅱ和Ⅲ-Ⅳ级脑胶质瘤组织中P21蛋白阳性表达率分别为69.6％及33.3%,而survivin蛋白阳性表达率分别为34.8%和72.2%,Ⅲ-Ⅳ级脑胶质瘤组织中P21蛋白阳性表达率明显低于Ⅰ-Ⅱ级,而survivin蛋白的阳性表达率明显高于Ⅰ-Ⅱ级(P<0.05)。结论:P21蛋白的表达缺失和survivin蛋白的过度表达与脑胶质瘤恶性程度有关联,P21蛋白有抑制脑胶质瘤发生发展的作用,而survivin蛋白在脑胶质瘤的恶变过程中起促进作用。     

胃癌Survivin 、COX-2 蛋白的表达及其相关性分析

[目的]探讨胃癌中Survivin蛋白表达与胃癌生物学行为及凋亡相关蛋白COX-2和凋亡指数(AI)的相关性.[方法]应用免疫组化SP法检测51例胃癌组织、47例非癌胃黏膜组织中Survivin和COX-2的表达,应用TUNEL法检测AI.[结果]Survivin蛋白在胃癌组织中的阳性表达率为70.58%较非癌胃黏膜组的36.17%明显增高(P＜0.05),COX-2在胃癌组织中的阳性表达率为68.63%较非癌胃黏膜组的30.04%明显增高(P＜0.05).Survivin蛋白在有淋巴结转移组阳性表达率显著高于无淋巴结转移组(94.59%vs 28.57%,P＜0.05),侵及浆膜组阳性表达率明显高于未及浆膜组(78.04%vs 40.00%,P＜0.05),且在Ⅲ-Ⅳ期胃癌病人Survivin阳性表达率明显高于Ⅰ～Ⅱ期(88.57%vs 31.25%,P＜0.05),COX-2蛋白在淋巴结转移组阳性表达率明显高于无淋巴结转移组(78.38%vs28.57%,P＜0.05),且Ⅲ-Ⅳ期病人阳性表达率明显高于Ⅰ～Ⅱ期(77.14%vs 37.50%,P＜0.05).Survivin阳性表达与COX-2阳性表达呈正相关(rs=0.32,P＜0.05).Survivin阳性组的平均AI明显低于阴性组(P＜0.05),而COX-2阳性组的平均AI明显高于阴性组(P＜0.05).[结论]Survivin和COX-2蛋白与胃癌的发生及其恶性演变有关,检测Survivin和COX-2蛋白有利于判断胃癌的恶性程度和预后.     

凋亡抑制基因Survivin在胃癌中的表达及临床意义

目的:研究凋亡抑制基因Survivin在胃癌组织中的表达及其临床意义.方法:应用免疫组织化学SP法,检测Survivin在30例胃癌组织,10例癌旁正常组织中的表达.结果:①胃癌组Survivin蛋白表达率为70.0%(21/30),显著高于正常胃组0%(0/10),P＜0.05.②淋巴结转移组Survivin蛋白阳性表达率(80.0%,16/20)显著高于无淋巴转移组(50.0%,5/10),P＜0.05;⑤根据临床病理分期,Ⅰ+Ⅱ期组(64.3%,9/14)与Ⅲ+Ⅳ组(62.5%,10/16)期之间Survivin蛋白阳性表达率差异有统计学意义(P＜0.05);随临床分期增加、淋巴结的转移,Survivin蛋白阳性表达率逐渐升高(P＜0.05).结论:Survivin的异常表达与胃癌的发生、发展密切相关,可能是影响胃癌预后的重要分子指标.     

亲环素A蛋白表达水平与人脑胶质瘤分级相关性研究

目的:探讨亲环素A(CypA)蛋白表达水平与人脑胶质瘤分级之间的关系.方法:采用免疫组化ABC法观察不同病理级别人脑胶质细胞瘤标本45例和正常脑组织10例中CypA蛋白表达情况,比较各级之间表达率的差异.结果:正常脑组织CypA蛋白表达率为0,45例脑胶质瘤组织CypA蛋白总阳性表达率为60.0%,较正常脑组织有显著差异;各级脑胶质瘤CypA蛋白表达阳性率分别为Ⅰ级16.7%,Ⅱ级40.0%,Ⅲ级69.2%,Ⅳ级81.3%,低级别(Ⅰ～Ⅱ级)组为31.3%.高级别(Ⅲ～Ⅳ级)组为75.9%,两组之间CypA蛋白表达有显著差异(P＜0.01).结论:CypA蛋白的表达水平与人脑胶质瘤病理分级相关.     

脑胶质瘤中Survirvin、微血管密度的表达及其相关性研究

目的探讨Survirvin蛋白、微血管密度(MVD)在人脑胶质瘤中的表达及其相关性.方法采用免疫组织化学SP法检测5例正常脑组织和43例胶质瘤组织中Survirvin、MVD的表达.结果Survirvin蛋白在正常脑组织中无表达,在43例胶质瘤组织中蛋白阳性表达26例,阳性率60.5%.Ⅰ～Ⅱ级和Ⅲ～Ⅳ级间的表达有显著性差异(P＜0.05),同时Survirvin的表达与MVD密切相关(r=0.704,P＜0.01).结论Survirvin蛋白异常表达在胶质瘤的发生中起重要作用,并与肿瘤血管形成及肿瘤的病理分级密切相关.     

胰腺癌组织中乙酰肝素酶蛋白的表达

目的:研究胰腺癌组织中乙酰肝素酶(heparanase,HPA)蛋白的表达及其临床意义.方法:应用免疫组织化学SP法检测27例胰腺癌组织(Ⅰ期4例,Ⅱ期8例,Ⅲ期11例,Ⅳ期4例;有淋巴结转移者18例,无淋巴结转移9例)、11例癌旁组织中HPA蛋白的表达情况,并对其表达与临床病理特征进行分析.结果:胰腺癌组织中HPA蛋白阳性表达率为70.1%(19/27),高于癌旁组织(9.1%,1/11)(P＜0.05).淋巴结转移组HPA蛋白阳性表达率(83.3%,15/18)高于无淋巴转移组(55.6%,5/9)(P＜0.05).Ⅰ Ⅱ期组HPA蛋白阳性表达率(66.7%,8/12)低于Ⅲ Ⅳ组(80.0%,12/15)(P＜0.05).结论:HPA的异常表达与胰腺癌的发生、发展密切相关,可能是影响胰腺癌预后的重要分子指标.     

连接蛋白43、胰岛素样生长因子Ⅰ在人脑胶质瘤的表达及其相关性

目的探讨缝隙连接蛋白43(Cx43)、胰岛素样生长因子Ⅰ(IGF-Ⅰ)在人脑胶质瘤的表达及其相关性。方法采用免疫组化SP法检测45例胶质瘤组织和10例正常脑组织Cx43、IGF-Ⅰ的表达。结果Cx43在正常脑组织和Ⅰ、Ⅱ级胶质瘤中表达水平高,Ⅲ、Ⅳ级表达水平低或缺失,在胶质瘤Ⅰ～Ⅱ级与Ⅲ～Ⅳ级之间的表达有极显著性差异(P＜0．01;IGF-Ⅰ在胶质瘤中的表达明显高于正常脑组织,且Ⅰ～Ⅱ级与Ⅲ～Ⅳ级的表达有显著性差异(P＜0．05),与Cx43的表达呈明显负相关(r5=0．688,P＜0．01)。结论IGF-Ⅰ表达随脑肿瘤恶性程度的增高而升高,表明IGF-Ⅰ可作为反映肿瘤细胞增殖能力的指标,它与Cx43的表达呈明显负相关,推测Cx43基因是通过抑制IGF-Ⅰ的表达来实现对细胞增殖的抑制。     

Survivin在人脑胶质瘤中的表达及预后的相关性研究

目的 探讨Survivin基因在人脑胶质瘤中的表达及其与胶质瘤病理分级、年龄及预后的相关性。方法采用免疫组织化学SABC法检测Survivin蛋白在41例胶质瘤及10例正常脑组织中的表达。结果Survivin蛋白在正常脑组织中无表达，41例胶质瘤中25例表达阳性，占61．0％，临床病理分级Ⅰ～Ⅱ、Ⅲ～Ⅳ级差异有显著性(P＜0．05)，Survivin表达阳性组与阴性组中患者两年生存率差异有显著性(P＜0．05)。结论Survivin蛋白表达的异常在胶质瘤的发展中起重要作用，Survivin基因与肿瘤的病理分级、患者预后密切相关，与患者的年龄无关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.