精子DNA碎片率与体外受精实验室结局的关系

目的初步探讨精子DNA碎片率、精子核蛋白不成熟度与体外受精实验室结局的关系。方法对2013年5~7月在我院行IVF治疗的男性患者行精液常规、精子DNA碎片率及精子核蛋白不成熟度检查,利用spearman相关分析探讨它们与IVF受精率、优胚率的关系。结果精子DNA碎片碎与男方年龄正相关,与前向运动精子百分率、优胚率负相关,与IVF受精率无相关。精子核蛋白不成熟度与精子浓度、正常精子百分率负相关,与IVF受精率、优胚率无显著相关。结论精子DNA碎片率检测对预测IVF优胚率有一定的临床意义。     

白血病人白细胞膜刀豆凝集素及麦芽胚凝集素受体分布的观察

本文报道人白血病(3例单核细胞性,1例毛细胞性、3例淋巴细胞性)白细胞膜刀豆凝集素(ConA)及麦芽胚凝集素(WGA)受体分布.白细胞膜表面均有WGA及ConA受体,WGA受体明显多于ConA受体.白血病的类型及分化程度不同,金颗粒在细胞膜表面的分布也有显著差异(1)毛细胞性白血病及淋巴细胞性白血病细胞膜上,WGA及ConA受体的分布多于单核细胞性白血病.(2)白血病白细胞膜上受体的多少与细胞分化程度成正相关,分化越低,受体数越少.文章对上述受体的分布进行了讨论.     

精子形态与顶体酶活性关系的研究

目的探讨精子形态与精子顶体酶活性的关系。方法对395例男性不育患者采用精子形态检测系统下人工修正方法分析精子形态,用酶标法检测精子中的顶体酶和顶体酶活力指数（AAI）。结果精子形态正常组的顶体酶活性明显高于精子形态异常组,两组差异显著（P〈0.05）;顶体酶活性正常组形态正常精子百分率明显高于顶体酶活性异常组形态正常精子百分率,两组间较差异极显著（P〈0.001）;顶体酶活性与形态正常精子百分率间呈显著正相关关系（r=0.169,P〈0.001）。结论精子形态可影响精子顶体酶活性。     

精子运动对体外受精率、妊娠率的影响

在体外受精-胚胎移植技术(In Vitro Fertilization-Embryo Transfer简称IVF-ET)中,过去单纯考虑或更多的考虑卵子质量,是因为得到卵子比较困难,超排卵花费较高;胚胎学方面也较多地强调卵子在胚胎发育中占有的作用,所以人们常常忽视精子质量特别是其运动能力对发育的影响,越来越多的事实说明,精子对IVF-ET的成功率同样起到至关重要的作用.而在20世纪90年代产生的计算机辅助分析精子(Computer Assisted Sperm Analysis CASA)技术则能比较准确地反映精子的运动特性.     

精子顶体酶活性与体外受精关系的探讨

目的探讨精子顶体酶活性与体外受精的关系。方法将236个体外受精-胚胎移植（IVF-ET）治疗周期根据顶体酶活性分成三组：A组≥48.2μIU/106精子,共95周期;B组：30～48.1μIU/106精子,共92周期;C组〈30μIU/106精子,共49周期;比较三组间的受精率、卵裂率、优质胚胎率。结果 A、B、C组的受精率分别为77.02%、74.11%和48.69%,卵裂率分别为97.80%、96.65%和90.29%,优质胚胎率分别为41.32%、40.79%和33.42%。三组间卵裂率、优质胚胎率比较,差异无统计学意义（P〉0.05）。C组的受精率明显低于A组（P〈0.05）,且完全不受精的发生率高于A、B组（P〈0.05）。B组与C组相比受精率下降,但差异无统计学意义（P〉0.05）。结论当顶体酶活性低于30μIU/10^6精子时受精率显著下降,可考虑行部分卵胞浆内单精子注射治疗。     

C型凝集素受体CLEC-2的研究进展

C型凝集素样受体-2(CLEC-2)是一个非经典型的C型凝集素受体,主要表达在血小板、中性粒细胞等细胞表面,在马来西亚蝮蛇蛇毒蛋白rhodocytin和跨膜唾液酸糖蛋白poloplanin等相关配体的刺激下,能通过细胞质尾部的YXXL结构域形成二聚体,从而激活syk依赖的信号传导.最近有很多研究显示:CLEC-2在血小...     

花生,大豆,刀豆凝集素受体与宫颈腺癌的关系

花生、大豆、刀豆凝集素受体与宫颈腺癌的关系马中民王美清王维屏采用花生（ＰＮＡ）、大豆（ＳＢＡ）和刀豆（ＣｏｎＡ）三种凝集素亲和组织化学染色，探讨宫颈腺癌与正常宫颈腺上皮和子宫内膜腺癌间此三种凝集素受体的差异以及它们对临床诊断工作的意义。并且对免疫组织...     

凝集素受体在叶酸,维生素C拮抗环磷酰胺致神经管畸形中的作用研究

为探讨凝集素受体在叶酸，维生素Ｃ防止歪磷酰酰胺致大鼠胚胎神经管畸形中的作用。方法：实验组和对照组孕鼠均于给环磷酰胺后２４小时剖腹取胎，常规石蜡包埋切片，用刀豆凝集素，花生凝集素和荆豆凝集素为分子探针作亲合组织杂色。对两组胚胎神经管的神经上皮，界膜及神经管周围间充质相应糖基进行定性，定位和半定一的比较观察。     

A new test for the assessment of sperm- zona pellucida penetration: relationship with results of other sperm tests and fertilization in vitro

The spermatozoa of some patients attending for in-vitro fertilization(IVF) fail to penetrate the zona pellucida in vitro. A testhas been devised to identify these cases. It is based on thenumber of spermatozoa penetrating into the zona pellucida, whichwere counted after removing spermatozoa bound to the zona surfaceby vigorous aspiration of each oocyte through a narrow gauge(120 µm) glass pipette. The oocytes were collected from197 patients undergoing IVF treatment with their own gametes;79 with no oocytes fertilized and 118 with some oocytes fertilized.Sperm motility, morphology and DNA normality (acridine orangestain) were also measured. The relationships between sperm testresults and IVF rate were examined by logistic regression. Theproportions of penetrated zonae, normal sperm morphology andnormal DNA were the most significant factors related to IVFrate in the whole group. Also, in patients with 30 spermatozoabound per zona pellucida or with normal sperm morphology 30%,the proportion of penetrated zonae and normal DNA were mostsignificant. Oocytes from 42 patients who had zero fertilizationand low sperm-zona binding (average, 2.2 spermatozoa/zona pellucida)were re-incubated with normal donor spermatozoa: large numbersof spermatozoa bound (average, 88 spermatozoa/zona pellucida)and each zona was penetrated by at least one spermatozoon. Inconclusion, the percentage of zonae penetrated was the variablemost significantly correlated with IVF rate. Penetration ofthe zona was also strongly related to fertilization rates inpatients without defects of sperm morphology and sperm-zonabinding. In patients where all zonae were penetrated, poor fertilizationmay be due to sperm morphology and DNA abnormalities. Failureof sperm-zona binding and penetration in vitro in patients withfailure of fertilization was mainly due to sperm defects andnot oocyte defects     

精子形态与活率相关性的研究及其在男性不育中的应用

目的探讨精子形态与活率的相关性,以便为男性不育的诊断提供理论指导。方法分别利用改良的巴氏染色法和伊红染色法,对精子形态和精子活率进行分析。结果与精子形态正常组相比,畸形精子组中精子活率异常的比例明显上升,差异具有统计学意义(P<0.05)。结论精子活率与精子形态呈正相关。精子形态可影响精子活率,进而影响男性的生育。     

Exposure of actin on the surface of the human sperm head during in vitro culture relates to sperm morphology, capacitation and zona binding

BACKGROUND: The aim of this study was to determine the relationship between the proportion of motile sperm with actin exposed on the surface of the head and sperm function. METHODS: Semen samples were obtained from normozoospermic men and sperm function tests were performed. Motile sperm selected by swim-up were incubated with actin monoclonal antibody (A-mAb, 1:100) for 2 h, then anti-mouse IgG Dynabeads were used to detect sperm-bound A-mAb. Sperm capacitation was increased by phorbol myristate acetate (PMA) and decreased by bicarbonate-free medium. RESULTS: The proportion of sperm with exposed actin increased with time for up to 2 h incubation. Bicarbonate-free medium significantly decreased the proportion of sperm with exposed actin. PMA significantly enhanced this phenomenon. Sperm bound to zona pellucida (ZP) had a significantly higher proportion with exposed actin than did sperm remaining in medium. Of the 79 samples studied, an average of 9.4% (range 1-27%) of motile sperm had exposed actin after 2 h incubation and this was significantly correlated with sperm normal morphology and ZP binding. CONCLUSION: Exposure of actin on the surface of the sperm head during in vitro culture may be related to membrane modification during sperm capacitation and hence may be a useful marker for this subpopulation of sperm.     

Effects of the testicular feminization mutation (tfm) of the androgen receptor gene on BSTMPM volume and morphology in rats

The posteromedial bed nucleus of the stria terminalis (BSTMPM) is an important component of the extended amygdala that is sexually dimorphic in rats. We examined the effect of the testicular feminization mutation (tfm), which renders the androgen receptor (AR) dysfunctional, on BSTMPM volume and average somal area. As expected, we found a significant sex difference in the volume of the BSTMPM, with females having a smaller BSTMPM than wild type males. Size of the BSTMPM in tfm males was also significantly smaller than that of wildtype males, although this difference was significant only on the left side. We found no sex difference in BSTMPM somal areas. These findings support the role of androgen receptors in the sexual differentiation of this nucleus.     

Expression of fibroblast growth factor receptor 1, fibroblast growth factor 2, phosphatidyl inositol 3 phosphate kinase and their clinical and prognostic significance in early and advanced stage of squamous cell carcinoma of the lung

Aim: Non-small cell lung carcinoma is the leading cause of cancer related to death in the world. Squamous cell lung carcinoma (SqCLC) is the second most frequent histological subtype of lung carcinomas. Recently, growth factors, growth factor receptors, and signal transduction system-related gene amplifications and mutations are extensively under investigation to estimate the prognosis and to develop individualized therapies in SqCLC. In this study, besides the signal transduction molecule phosphatidyl inositol-3-phosphate kinase (IP3K) p110α, we explored the expressions of fibroblast growth factor 2 (FGF2) and receptor-1 (FGFR1) in tumor tissue and also their clinical and prognostic significance in patients with early/advanced SqCLC. Materials and methods: From 2005 to 2013, 129 patients (23 early, 106 advanced disease) with a histopathological SqCLC diagnosis were selected from the hospital files of Cukurova University Medical Faculty for this study. Two independent pathologists evaluated FGFR1, FGF2, and PI3K (p110α) expressions in both tumor and stromal tissues from 99 of the patients with sufficient tissue samples, using immunohistochemistry. Considering survival analysis separately for patients with both early and advanced stage diseases, the relationship between the clinical features of the patients and expressions were evaluated by univariate and multivariate analyses. Results: FGFR1 expression was found to be low in 59 (60%) patients and high in 40 (40%) patients. For FGF2; 12 (12%) patients had high, 87 (88%) patients had low expression and for IP3K; 31 (32%) patients had high and 66 (68%) patients had low expressions. In univariate analysis, overall survival (OS) was significantly associated with stage of the disease and the performance status of the patient (P<0.0001 and P<0.001). There was no significant difference in OS of the patients with either low or high expressions of FGFR1, FGF2, and IP3K. When the patients with early or advanced stage disease were separately taken into consideration, the relationship did not differ, either. Any of FGFR1, FGF2 or IP3K expressions was not found predictive for the treatment of early or advanced staged patients. On the other hand, the expressions of both FGFR1 and FGF2 were significantly different with respect to smoking, scar of tuberculosis and scar of radiotherapy (P=0.002; P=0.06 and P=0.05, respectively). Discussion: There has not been identified an effective individualized treatment for SqCLC yet. Therefore, in order to be able to develop such a treatment in the future, it is essential to identify the genetic abnormalities that are responsible for the biological behaviors and carcinogenesis of SqCLC. Although we could not show the prognostic and predictive significance of FGFR1, FGF2 and IP3K expressions in SqCLC, we determined the expression rates of FGFR1, FGF2 and IP3K as a reference for Turkish patients. In conclusion, we want to put some emphasis on the fact that, pulmonary fibrosis which is a late complication of radiotherapy at stage III disease, and the scar of tuberculosis could be associated with FGFR1 and FGF2 expressions.