IL-10-819位点基因多态性与汉族人群重度慢性牙周炎易感性相关性的研究

目的　探讨IL 10启动子区基因多态性与重度慢性牙周炎易感性的关系。方法　收集 14 2名重度慢性牙周炎患者和 81名健康对照者的颊粘膜拭子 ,提取DNA ,采用ASO PCR方法检测IL 10 819位点基因多态性 ,比较重度慢性牙周炎患者和健康对照组中等位基因频率和基因型分布。结果　IL 10 819C/T等位基因频率和基因型分布在患者和对照组之间差异无显著性 (OR =1.377,P =0 .12 2 >0 .0 5 )。结论　IL 10 819位点基因多态性与汉族人群重度慢性牙周炎遗传易感性无相关关系。     

白细胞介素-17基因多态性与侵袭性牙周炎的相关性研究

目的已有少量文献证实白细胞介素-17(interleukin-17,IL-17)基因多态性与多种免疫炎症性疾病相关。本研究旨在探索中国汉族人群中,IL-17A(rs2275913)和IL-17F(rs763780)位点单核苷酸多态性(single nucleotide polymorphisms,SNPs)与侵袭性牙周炎(aggressive periodontitis,Ag P)的相关性。方法采集87例Ag P、79例牙周健康者的外周静脉血,提取基因组DNA,采用多重SNa Pshot技术分析待测位点的基因多态性。结果 IL-17A(rs2275913)位点的基因型分布、IL-17F(rs763780)位点的基因型和等位基因分布在Ag P组和健康对照(healthy controls,C)组中差异均无统计学意义(P≥0.05)。IL-17A(rs2275913)位点在Ag P组中A等位基因频率显著高于健康对照组(54.0%vs.42.4%,P=0.034,OR=1.596,95%CI=1.034-2.463)。结论研究结果显示IL-17A(rs2275913)位点A等位基因可能是Ag P发病的危险因素,提示IL-17A(rs2275913)位点的多态性可能与中国汉族人群的Ag P易感性有关。     

白细胞介素8基因-251位点多态性与侵袭性牙周炎的相关性研究

目的研究白细胞介素8（interleukin-8,IL-8）-251位点基因多态性与侵袭性牙周炎易感性的相关性。方法采用病例对照试验设计,从广东汉族人群中选择77例侵袭性牙周炎（aggressive periodontitis,AgP）患者（AgP组）及50例牙周健康者（健康对照组）,采用聚合酶链反应—限制性内切酶片段长度多态性方法对IL-8-251位点基因进行检测,分析组间等位基因和基因型频率的分布差异。结果 IL-8-251A/T位点的基因型和A、T等位基因频率在AgP组和健康对照组的分布差异无统计学意义（P〉0.05）。结论未发现IL-8-251A/T位点基因多态性与中国广东汉族人群侵袭性牙周炎的患病易感性之间存在相关性。     

白细胞介素-1基因多态性与侵袭性牙周炎的关系

目的：研究中国人群中白细胞介素-1（IL-1）基因多态性与侵袭性牙周炎（AgP）之间的关系。方法：提取122例AgP患者和95例健康对照者外周静脉血基因组DNA,采用聚合酶链反应（PCR）分析IL-1A＋4845、IL-1B＋3954位点的单核苷酸多态性（SNP）及IL-1RN第二内含子中的可变数目重复序列（VNTR）多态性,采用多因素Logistic回归模型,进行两组之间基因型、等位基因分布差异的比较。结果：在IL-1A＋4845位点,AgP组男性患者A2^＋基因型的频率较男性健康组显著升高（P〈0．05=0．039）,等位基因A2的携带率也显著升高（P〈0．05=0．049）;未发现IL-1B＋3954位点和IL-1RN VNTR的多态性与AgP关联;未发现IL-1各复合基因型与AgP存在明显关联性。结论：IL-1A＋4845位点的SNP可能与中国人群中男性个体的AgP易感性有关。     

IL-6-572基因多态性与侵袭性牙周炎易感性的相关性研究

目的 研究白细胞介素-6(interleukin-6,IL-6)-572位点基因多态性与侵袭性牙周炎易感性的关系.方法 采用病例对照试验设计,从广东汉族人群中选择83例侵袭性牙周炎(aggressive periodontitis,AgP)患者(AgP组)及79例牙周健康者(对照组),采用聚合酶链反应—限制性内切酶片段长度多态性分析方法对IL-6-572位点基因多态性进行检测,分析组间基因型频率及等位基因分布的差异.结果 IL-6基因启动子区-572位点G/C基因型在AgP组、对照组中的分布频率差异有统计学意义(x2=13.710,P=0.001).AgP组与对照组相比,G、C等位基因频率分布差异有统计学意义(x2=13.213,P<0.001),G等位基因相对于C等位基因:OR值为2.988,95%CI:1.634~5.465.结论 IL-6-572 G/C位点的基因多态性同中国广东汉族人群侵袭性牙周炎患病易感性可能存在相关关系,IL-6-572 G等位基因可能是广东汉族人群AgP遗传易感性的高风险因素.     

维生素D受体基因多态性与汉族人群慢性牙周炎易感性的关系

目的 探讨维生素D受体(VDR)基因多态性与汉族人群慢性牙周炎(CP)易感性的关系。方法 收集汉族轻、中、重度CP患者共166例及80名无牙周炎对照者的颊黏膜拭子，以Chelex-100法提取DNA，采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定VDR BsmI、VDR ApaI和VDR TaqI的基因型，分析组间基因型分布和等位基因频率的差异。结果 轻、中、重度CP患者中VDR ApaI等位基因A携带者明显多于对照组，重度CP与中度CP、重度CP与轻度CP患者间VDR ApaI基因型的分布差异均有统计学意义，中度CP与轻度CP患者间VDR ApaI基因型分布差异无统计学意义，而VDR BsmI、TaqI位点的基因型分布在患者组和对照组间差异无统计学意义。结论 VDR ApaI等位基因A可能与汉族人群CP的易感性有关。     

HMGB1基因多态性与中国汉族人群慢性牙周炎易感性的关系研究

目的:探讨高迁移率族蛋白B1(high mobility group protein B1,HMGB1)基因多态性与中国汉族人群慢性牙周炎(chronic periodontitis, CP)易感性的关系。方法:选取150例CP患者作为CP组,健康体检正常者100例作为对照组。检测所有研究对象HMGB1基因单核苷酸多态性(single nucleotide polymorphism, SNP)位点多态性,应用Logistic回归分析HMGB1基因多态性与CP发病的关系。结果:CP组HMGB1基因rs2249825位点CC、CG、GG基因型分布频率与对照组比较,差异有统计学意义(P<0.05)。CP组HMGB1基因rs2249825位点G等位基因频率与对照组比较,差异有统计学意义(P<0.05)。在显性模式和隐性模式中,CP组HMGB1基因rs2249825位点基因型分布与对照组比较有统计学差异(P<0.05),与CC基因型比较,CG和GG基因型与CP发病有关(P<0.05)。Logistic回归分析显示,rs2249825位点基因多态性与CP发病相关,且CG/G...     

雌激素受体XbaI和PvuⅡ基因多态性与侵袭性牙周炎的相关研究

目的:探讨侵袭性牙周炎(aggressive periodontitis,AgP)易感性与雌激素受体XbaI和PvuⅡ基因多态性的关系.方法:收集48 例侵袭性牙周炎(AgP)患者和60 例牙周健康对照组的颊黏膜拭子,采用Chelex-100法提取DNA,应用聚合酶链式反应-限制性片段长度多态性技术检测雌激素受体XbaI和PvuⅡ的等位基因及基因型频率在各组之间分布的差异.结果:XbaI基因型分布在AgP组与对照组、AgP组女性与对照组女性、AgP组男性与对照组男性之间均有显著性差异(P<0.05).其等位基因频率在女性组间有统计学意义(P<0.05). PvuⅡ基因型分布在AgP组与对照组之间均无明显差异,其等位基因频率分布有明显差异(P<0.05). 多因素logistic回归分析显示男性患病的可能性小(OR=0.352);随年龄增大患病的可能性变小(OR=0.950);当其他条件一定时,xx、Xx基因型的人患病的可能性小(OR 值分别为0.678,0.224).结论:侵袭性牙周炎易感性与雌激素受体XbaI基因型分布有关,XX基因型最易患病,其次为xx,Xx基因型.     

上海地区汉族慢性牙周炎患者与基因组单核苷酸多态性的相关性研究

目的:研究7个基因中的12个单核苷酸多态性(single nucleotide polymorphisms,SNPs)与慢性牙周炎的相关性,筛查上海地区汉族慢性牙周炎患者相关易感基因的SNP位点.方法:应用高通量基因分型质谱技术(MALDITOF MS system),对上海地区汉族人群所选定的SNP进行分型,研究人群为随机入组的50例牙周正常者和48例慢性牙周炎患者,按照统一设计的问卷格式,收集病史、家族史、体征、实验室检查、治疗、随访等资料.应用SPSS 13.0软件包对数据进行x2检验.结果:9p21.3区域的rs2891168的GG基因型与慢性牙周炎显著相关,OR=3.071(95%CI:1.018～9.260,P=0.046).结论:rs2891168中的GG与上海地区汉族人群的慢性牙周炎发病具有显著相关性.     

白细胞介素-6基因-572C/G多态性与慢性牙周炎的相关性研究

目的研究白细胞介素-6（interleuk in-6,IL-6）基因启动子区域-572C/G位点基因多态性与慢性牙周炎易感性的关系。方法采用病例对照试验设计,轻中度牙周炎组87例,重度牙周炎组72例,健康对照组90例,收集颊粘膜拭子,使用聚合酶链反应—限制性内切酶片段长度多态性基因分析的方法,检测各组IL-6-572位点基因型和等位基因分布。结果 IL-6基因-572C/G位点G等位基因的检出率在健康组是14.4%,轻中度牙周炎组是14.4%,重度牙周炎组是20.1%。等位基因频率在患者和健康者之间无统计学差异（P=0.287）。CC基因型在各组的分布分别是健康组73.3%,轻中度牙周炎组71.3%,重度牙周炎组63.9%,各组之间差异无统计学意义（P=0.308）。结论 IL-6基因-572位点多态性与汉族人群慢性牙周炎的易感性无明显相关。     

非手术方法治疗侵袭性牙周炎临床疗效观察

目的探讨非手术方法治疗侵袭性牙周炎的临床效果。方法选择广泛型侵袭性牙周炎患者15例,进行口腔卫生宣教、龈上洁治、龈下刮治和根面平整,并蹲服罗红霉素和甲硝唑1周。分别于治疗前和治疗后3、6、12、18个月检查及记录出血指数、探诊深度和附着水平,并进行分析比较。结果出血指数、探诊深度和附着水平在治疗前分别为3．37±0．56、（5.83±1．68）mm．（6．78±1．50）mm,治疗后18个月下降为（0．69±0．48）mm、（2．15±0．45）mm、（4．60±0．78）mm,差异均有统计学意义（P=0.000）。结论广泛型侵袭性牙周炎患者经过牙周非手术治疗后可以取得良好的治疗效果,并且疗效较为稳定。     

Detection of Eight Periodontal Microorganisms and Distribution of Porphyromonas gingivalis fimA Genotypes in Chinese Patients With Aggressive Periodontitis

Background: The microbiologic feature of aggressive periodontitis (AgP) in Chinese patients has not yet been determined. This study aims to investigate the prevalence of eight periodontal microorganisms and the distribution of the Porphyromonas gingivalis fimA genotype in a cohort of Chinese patients with AgP. Methods: Saliva and pooled subgingival plaque samples were collected from 81 patients with AgP (25 with incisor–first molar type and 56 with generalized type [GAgP]) and 34 periodontally healthy controls. Eight periodontal microorganisms, including Aggregatibacter actinomycetemcomitans, P. gingivalis, Tannerella forsythia, Treponema denticola, Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens, and Fusobacterium nucleatum were detected in these samples by the polymerase chain reaction (PCR). In addition, the distribution of fimA genotypes was assessed in P. gingivalis–positive individuals by PCR. Results: The prevalence of P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, F. nucleatum, and A. actinomycetemcomitans in patients with AgP was significantly higher than that in healthy controls. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low (30.4%) compared with other pathogens. Results of logistic regression analysis showed that younger patients were more likely to harbor A. actinomycetemcomitans (odds ratio = 2.85). Type II was the most prevalent fimA genotype of P. gingivalis in patients with AgP. Conclusions: P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, and F. nucleatum were the predominant periodontal pathogens of patients with GAgP in China. Type II of fimA was the most prevalent genotype of P. gingivalis in patients with AgP. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low.     

Salivary and Serum Markers Related to Innate Immunity in Generalized Aggressive Periodontitis

Background: Host inflammatory and immune responses play an important role in aggressive periodontitis (AgP). Thus, this study aims to evaluate levels of the innate immunity‐related markers calprotectin, colony‐stimulating factor (CSF)‐1, macrophage migration inhibitory factor (MIF), monokine induced by interferon‐γ (MIG), and matrix metalloproteinase (MMP)‐8 in serum and saliva from patients with generalized AgP and those with gingivitis or a healthy periodontium. Methods: This study enrolled 40 individuals (17 males and 23 females; mean age 33.30 ± 9.31 years), 15 with generalized AgP, 15 with gingivitis, and 10 who were periodontally healthy. Full‐mouth periodontal examinations were performed, and serum and saliva were collected. Levels of calprotectin, CSF‐1, MIF, MIG, and MMP‐8 were measured using enzyme‐linked immunosorbent assays. Results: In serum, mean levels of calprotectin were 2.06‐fold higher in patients with AgP than in healthy patients (P = 0.01). Serum levels of MMP‐8 were significantly elevated in patients with AgP compared with both healthy patients and those with gingivitis, by 2.60‐fold and 2.77‐fold, respectively (P = 0.03 and P = 0.009, respectively). In saliva, levels of MMP‐8 were 5.66‐fold higher in patients with AgP than in healthy patients (P = 0.02). CSF‐1, MIF, and MIG levels in both serum and saliva did not differ significantly among the groups. Conclusions: Serum levels of calprotectin and MMP‐8 are elevated in patients with AgP. MMP‐8 levels are also increased in saliva from patients with AgP. These results support involvement of innate immune response in the pathogenesis of AgP.     

Suppuration‐Associated Bacteria in Patients With Chronic and Aggressive Periodontitis

Background: Suppuration (SUP) on probing may be an indication of active periodontal breakdown. The aim of the present study is to analyze which subgingival species are associated with SUP in patients with chronic (CP) and aggressive (AgP) periodontitis. Methods: A total of 156 patients with CP and 66 with AgP were submitted to full‐mouth periodontal examination and subgingival biofilm sampling (14 sites/patient). The counts of 44 bacterial species were determined by checkerboard. Comparisons between groups and sites were analyzed by the Mann‐Whitney and Wilcoxon tests, respectively. Associations between frequency of SUP and bacterial species were analyzed by the Spearman correlation coefficient. Results: The prevalence of SUP in patients with CP was 24.4%, and in patients with AgP it was 30.3%, and the percentage of SUP sites in the groups was 5.72% ± 1.06% and 6.96% ± 1.70%, respectively (P >0.05). SUP sites from patients with CP had significantly higher counts of Veillonella parvula, Dialister pneumosintes, Tannerella forsythia, and Prevotella nigrescens than SUP sites from patients with AgP (P <0.005). Significant positive correlations between high frequency of SUP and high levels of Actinomyces spp, Streptococcus spp., members of the orange complex, Acinetobacter baumannii, and Pseudomonas aeruginosa were observed in patients with CP (P <0.05). In patients with AgP, Actinomyces oris, Propionibacterium acnes, P. aeruginosa, Staphylococcus aureus, and Streptococcus sanguinis were positively associated with SUP, whereas Prevotella intermedia presented a negative association with SUP (P <0.05). Conclusions: SUP sites from patients with CP harbored significantly higher counts of several periodontal species than SUP sites from patients with AgP. Actinomyces spp., Streptococcus spp., members of the orange complex, T. forsythia, and certain non‐oral pathogens were associated with a high number of sites with SUP.     

伴放线放线杆菌在龈下菌斑和颊黏膜分布研究

目的:比较伴放线放线杆菌(actinobac illus actinomycetem com itans,A.a)在不同类型牙周炎患者龈下菌斑和颊黏膜中的分布。方法:通过聚合酶链反应(polym erase chain reaction,PCR)对侵袭性牙周炎患者(AgP)、慢性牙周炎患者(CP)、牙周健康者口腔龈下菌斑和颊黏膜中的A.a进行检测,分析该菌分别在两部位的相对含量。结果:AgP组菌斑和颊黏膜样本中A.a阳性检出率均为41.7%,分别高于CP组(菌斑16.7%、颊黏膜10.0%)和牙周健康组(菌斑和颊黏膜均为0%)。AgP组A.a在菌斑和颊黏膜的相对含量分别为38.5%和22.2%,高于CP组(菌斑19%、颊黏膜12.75%)。结论:A.a不仅存在于龈下菌斑中,也能够粘附于颊黏膜;A.a是AgP的主要优势菌也参与了CP的菌群组成。     

Interleukin-10 gene promoter polymorphism in Japanese patients with adult and early-onset periodontitis

BACKGROUND: IL-10 is an anti-inflammatory cytokine, which may modulate disease expression in chronic inflammatory periodontal disease. 3 dimorphic polymorphisms within the IL-10 gene promoter have recently been identified and appear to influence regulation of its expression. AIM: The aim of the present study was to investigate whether the promoter polymorphisms are associated with adult periodontitis (AP) and generalized early-onset periodontitis (G-EOP). METHODS: Genomic DNA was obtained from 34 AP patients, 18 G-EOP patients and 52 controls. The promoter region between -506 and -1140 was amplified by polymerase chain reaction, and polymorphisms were detected by nucleotide sequencing. RESULTS: The haplotype frequencies in Japanese were quite different from those of Caucasian and were even slightly different from those of southern Chinese with systemic lupus erythematosus. We found no significant difference in allele or haplotype frequencies between patients and controls. CONCLUSIONS: IL-10 production may be regulated within the complex cytokine network in chronic inflammatory periodontal disease, rather than the gene polymorphisms.     

New Attempts to Modify Periodontal Risk Assessment for Generalized Aggressive Periodontitis: A Retrospective Study

Background : Periodontal risk assessment (PRA) model was designed for risk evaluation of treated patients with periodontal disease. However, its use on generalized aggressive periodontitis (GAgP) had been scarcely reported. This study aims to investigate the association of original PRA/modified PRA (MPRA) and compliance of periodontal maintenance with long‐term treatment outcomes of Chinese patients with GAgP. Methods: Eighty‐eight patients from a GAgP cohort, who completed active periodontal treatment (APT) and accepted reevaluation 3 to 11 years (mean of 5.5 years) afterward, were enrolled. PRA was modified (three strategies involving replacement of bleeding on probing with bleeding index >2, counting sites with probing depth ≥6 mm and changing method of bone loss [BL] calculation) to classify patients into different risk groups based on data at the first recall after APT. PRA and three MPRA models were investigated regarding long‐term association with tooth loss (TL) and alteration of bone level (?BL). Results: Based on original PRA, 87 patients (98.8%) had a high‐risk profile. According to three MPRA models, annual TL per patient values were greater in high‐risk groups than in low‐to‐moderate risk groups (MPRA‐1, 0.20 ± 0.33 versus 0.04 ± 0.14; MPRA‐2, 0.18 ± 0.32 versus 0.05 ± 0.14; MPRA‐3, 0.17 ± 0.32 versus 0.05 ± 0.15; P <0.05). By MPRA‐1, irregular compliers with low‐to‐moderate risk profile had greater ?BL (0.027 ± 0.031, indicating bone increment) than those with high risk (?0.012 ± 0.064, tendency for BL). For regular compliers, no significant differences of annual TL or ?BL were found between risk groups. Conclusions: MPRA models could be used for evaluating the long‐term outcomes of Chinese patients with severe GAgP, especially irregular compliers. High‐risk patients of MPRAs exhibited more TL and less bone fill than low‐to‐moderate risk ones.