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Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymerase 总被引:13,自引:2,他引:13
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Stephanie H. Shanblatt Arnold Revzin 《Proceedings of the National Academy of Sciences of the United States of America》1983,80(6):1594-1598
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Demonstration of two operator elements in gal: in vitro repressor binding studies. 总被引:24,自引:9,他引:24
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A Majumdar S Adhya 《Proceedings of the National Academy of Sciences of the United States of America》1984,81(19):6100-6104
Genetic and DNA base sequence analyses of cis-dominant mutations that derepress the gal operon of Escherichia coli suggested the existence of two operator loci needed for gal repression. One (OE) is located immediately upstream to the two overlapping gal promoters and the other (OI) is inside the first structural gene. We have investigated the ability of wild-type and mutant OE and OI DNA sequences to bind to gal repressor. The repressor has been purified from cells containing a multicopy plasmid in which the repressor gene is brought under the control of phage lambda PL promoter. The DNA-repressor interactions are detected by the change in electrophoretic mobility of labeled DNA that accompanies its complex formation with repressor protein. The purified repressor shows concentration-dependent binding to both O+E and O+I but not to OEc and OIc sequences. These results authenticate the proposed operator role of the two homologous gal DNA control elements and thereby establish that the negative control of the gal operon requires repressor binding at both OE and OI, which are separated by greater than 90 base pairs. 相似文献
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Specific stimulation of simian virus 40 late transcription in vitro by a cellular factor binding the simian virus 40 21-base-pair repeat promoter element 总被引:12,自引:1,他引:12
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C H Kim C Heath A Bertuch U Hansen 《Proceedings of the National Academy of Sciences of the United States of America》1987,84(17):6025-6029
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Cyclic AMP-dependent constitutive expression of gal operon: use of repressor titration to isolate operator mutations. 总被引:12,自引:3,他引:9
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M Irani L Orosz S Busby T Taniguchi S Adhya 《Proceedings of the National Academy of Sciences of the United States of America》1983,80(15):4775-4779
When the gal operator region is present in a multicopy plasmid it binds to all ("titrates") the gal repressor and "induces" the chromosomal gal operon. To make operator mutations (Oa) with reduced affinity toward the repressor, plasmid DNA was irradiated with UV light and mutant derivatives were isolated that were unable to release the chromosomal gal genes from repression. Then with such an Oa plasmid operator revertants were isolated that had reacquired the ability to release repression. Both sets of mutations have been localized by DNA sequence analysis. When the Oa mutations were transferred from the plasmid to the chromosome by recombination these mutant operators were found to make gal expression constitutive (independent of repressor) but still dependent on cAMP, whereas the previously reported gal operator mutants (Oc) are constitutive both in the presence and in the absence of cAMP. The titration method of isolating mutants enables the isolation of strains with operator mutations that also affect normal promoter activity, and it provides an easy way to isolate revertants of operator mutations. 相似文献
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Adenovirus EIIA early promoter: transcriptional control elements and induction by the viral pre-early EIA gene, which appears to be sequence independent. 总被引:54,自引:10,他引:54
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S C Murthy G P Bhat B Thimmappaya 《Proceedings of the National Academy of Sciences of the United States of America》1985,82(8):2230-2234
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Single amino acid substitutions in the cAMP receptor protein specifically abolish regulation by the CytR repressor in Escherichia coli.
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L S?gaard-Andersen A S Mironov H Pedersen V V Sukhodelets P Valentin-Hansen 《Proceedings of the National Academy of Sciences of the United States of America》1991,88(11):4921-4925
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Two promoters, one inducible and one constitutive, control transcription of the Streptomyces lividans galactose operon 总被引:22,自引:1,他引:22
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J A Fornwald F J Schmidt C W Adams M Rosenberg M E Brawner 《Proceedings of the National Academy of Sciences of the United States of America》1987,84(8):2130-2134
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