牙周组织再生术与口腔正畸联合在牙周炎患者中的作用分析

目的总结并分析牙周炎患者应用牙周组织再生术与口腔正畸联合治疗的临床效果。方法研究对象都是在本院2016年10月至2018年3月接受治疗的74例牙周炎患者,以抽签法处理为牙周组织再生术与口腔正畸联合治疗的试验组(n=37例),单一口腔正畸治疗的对照组(n=37),比较两组患者的治疗效果。结果试验组治疗有效率以及临床各项指标均优于对照组,呈现出P0.05的最终结局,具有探讨研究价值。结论对牙周炎患者进行牙周组织再生术与口腔正畸联合治疗,可提升患者的临床治疗有效率,改善患者的龈沟出血指数、菌斑指数、牙周袋探诊深度以及临床附着丧失。     

正畸对口腔龈沟内牙周菌群的影响

目的:分析正畸治疗对口腔龈沟内牙周菌群的影响。方法:选择我院收治的75例采用固定矫治治疗的患者作为观察对象,回顾性分析患者的临床资料。结果:75例患者治疗后口腔内球菌检出结果明显低于治疗前,核梭杆菌水平则高于治疗之前,对比差异结果显著(P0.05);此外,患者的口腔中,卟啉单细胞菌的检出率同样高于正畸治疗之前,差异具有统计学意义(P0.05)。结论:正畸治疗之后口腔龈沟中细菌会出现较大的变化,临床医生应当予以充分的重视,及时进行干预,确保患者牙周健康。     

青少年正畸治疗后牙周组织状况的临床观察

目的:总结青少年患者固定正畸治疗结束时牙周状况。方法:河南省口腔医院正畸科固定正畸治疗结束后牙列整齐、尖窝关系良好的病例22例,于正畸结束去除托槽后随即应用简化牙周指数进行牙周检查,检测菌斑指数、牙石指数、探诊深度及探针出血等临床牙周指数。根据患者治疗结束时的牙周状况,与未行正畸治疗的对照组牙周情况比较。结果:观察组青少年各项牙周指数均高于对照组,PI、CI、PD、BI差异均有统计学意义(P<0.05)。结论:戴用固定矫治器对青少年患者的口腔卫生有一定的不良影响,此不良影响与多方面影响因素有关。     

丁硼乳膏治疗正畸牙龈炎的疗效观察

目的观察丁硼乳膏治疗正畸牙龈炎的临床效果。方法选择70例正畸治疗伴发牙龈炎的患者作为研究对象,随机分成实验组（丁硼乳膏刷牙）和对照组（普通牙膏刷牙）,每组35例,分别记录其治疗前和治疗后2,4周的牙周指数（牙龈指数GI、菌斑指数PLI、龈沟出血指数SBI）,并进行统计学分析。结果实验组治疗2、4周后CI、SBI、PU显著下降,与对照组比较差异有统计学意义（P均〈0．05）。结论丁硼乳膏对口腔中常见细菌有抑制作用,能有效维持固定正畸治疗中的牙周健康,防止牙龈炎的发生。     

正畸牙周联合治疗对牙周炎患者牙周指标的影响

目的：探讨正畸牙周联合治疗对牙周炎患者牙周指标的影响。方法：选取2020年7月-2021年6月重庆市大足区妇幼保健院口腔科收治的68例牙周炎患者作为研究对象,随机分为对照组与试验组,各34例。两组均进行4个月的牙周治疗,试验组在牙周症状明显改善后进行正畸治疗。比较两组牙周指数、临床疗效以及不良反应情况。结果：治疗前,两组龈沟出血指数、菌斑指数、牙龈指数及牙周袋探诊深度比较,差异无统计学意义(P>0.05);治疗后,两组各项牙周指数均降低,且试验组低于对照组,差异有统计学意义(P<0.05)。试验组治疗总有效率高于对照组,差异有统计学意义(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论：相较于单一牙周治疗,正畸、牙周联合治疗牙周炎的效果显著,可改善患者的牙周指标,且安全性好,是治疗牙周炎疾病的优选方案。     

成人正畸牙周炎患者龈沟液及血清中PAK5的检测与意义

目的 检测成人口腔正畸并发牙周炎的患者龈沟液及血清中p21活化激酶5(PAK5)的含量,并探讨其与牙周炎发病机制的关系.方法 采用双抗体夹心ABC-ELISA法检测67例口腔正畸治疗过程中并发牙周炎的成人患者与40例未并发牙周炎的同期患者龈沟液及血清中PAK5的含量,分析PAK5的检出率及在牙周炎治疗前后的变化,探讨其与牙周炎发病机制的可能联系.结果 正畸并发牙周炎患者龈沟液中的PAK5检出率及含量均高于未并发牙周炎者,牙周炎经治疗后龈沟液中的PAK5检出率及含量均降低,但较未并发牙周炎者仍为高;而血清中PAK5的检出率及含量两组之间及牙周炎组治疗前后均无显著差异.结论 PAK5的表达有可能参与了牙周炎的发病过程,在正畸性牙周炎的预防和治疗方面有可能成为新的靶点.     

高频电刀热凝治疗口腔颌面部海绵状血管瘤

目的探讨高频电刀热凝治疗口腔海绵状血管瘤的疗效.方法随机选择口腔海绵状血管瘤患者59例,其中上唇16例,下唇13例,颊部10例,舌部26例,牙龈4例,采用CV-2000电脑高频电刀治疗机治疗,观察疗效并随访1～3年.结果 59例患者中49例有效,1次治疗后痊愈;8例显效,1次治疗后痊愈;2例无效.结论高频电刀热凝治疗口腔海绵状血管瘤简单、疗效好、时间短.     

口腔健康教育对Ⅱ型糖尿病患者牙周病影响的观察

目的：探讨口腔健康教育对Ⅱ型糖尿病患者牙周病的影响。方法：选择80例患有牙周病的Ⅱ型糖尿病患者,随即分为两组。研究组40例给予牙周基础治疗和术后口腔健康教育,对照组40例单纯给予牙周基础治疗。分别在治疗前和治疗后1、3、6个月复查牙周指数的变化。结果：两组患者牙周基础治疗前其牙龈指数（GI）、牙菌斑指数（PLI）、牙周袋探诊深度（PD）、龈沟出血指数（SBI）、牙周附着丧失（AL）、牙齿松动度（TM）均无明显差异,术后1个月两组患者各项牙周指标与其基线比有显著性差异。研究组术后3、6个月其各项牙周指数与基线比仍有显著性差异。结论：口腔健康教育可有效维持2型糖尿病患者牙周病的治疗效果。     

口腔健康教育对Ⅱ型糖尿病患者牙周病影响的观察

目的探讨口腔健康教育对Ⅱ型糖尿病患者牙周病的影响.方法选择80例患有牙周病的Ⅱ型糖尿病患者,随即分为两组.研究组40例给予牙周基础治疗和术后口腔健康教育,对照组40例单纯给予牙周基础治疗.分别在治疗前和治疗后1、3、6个月复查牙周指数的变化.结果两组患者牙周基础治疗前其牙龈指数(GI)、牙菌斑指数(PLI)、牙周袋探诊深度(PD)、龈沟出血指数(SBI)、牙周附着丧失(AL)、牙齿松动度(TM)均无明显差异,术后1个月两组患者各项牙周指标与其基线比有显著性差异.研究组术后3、6个月其各项牙周指数与基线比仍有显著性差异.结论口腔健康教育可有效维持2型糖尿病患者牙周病的治疗效果.     

正畸牙齿移动过程中Pentraxin-3在龈沟液中表达水平的研究

目的:通过检测正畸牙齿移动过程不同时间段内龈沟液中PTX3的水平,分析PTX3在正畸治疗早期过程中的动态变化,并分析其潜在的临床意义。方法:对19例正畸口腔患者分别于正畸治疗前、正畸加力后4小时、12小时、24小时、48小时、7天、14天、1月收集龈沟液,用ELISA试剂盒检测龈沟液内PTX3表达水平。结果:在正畸治疗牙齿移动早期,正畸患者龈沟液中的PTX3的表达水平在正畸加力后上升,在正畸加力后4小时、12小时、24小时、48小时内的表达水平高于正畸治疗前(P<0.05),并于24小时达到峰值,后开始逐渐下降,于正畸治疗后一周恢复正常水平。在早期正畸牙齿移动过程中,PTX3水平与TNF-α、IL-1β均呈正性相关关系(r=0.69,P<0.05;r=0.58,P<0.05);PTX3的表达水平与患者的尖牙移动距离呈现负性相关关系(r=-0.58,P=0.02)。结论:作为机体重要的一类炎症因子,PTX3在正畸牙齿移动早期过程中的龈沟液内表达水平呈现先升高后下降的趋势,龈沟液中PTX3的水平可以有效反映正畸力作用下牙周组织的炎症状况,对其水平的检测将有助于判断正畸治疗效果,防止不良反应发生。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.