细胞角蛋白20检测对判断大肠癌淋巴结微转移的临床意义

Objective To study the significance of cytokeratin 20(CK20)in micrometastasis of lymph node of colorectal cancer.Methods The 331 lymph nodes in 47 cases with colorectal cancer by radical resection were collected from 2000 to 2007 in our Hospital.They were carried out CK20 immunohistochemical staining to determine the existence of micrometastasis,and all patients were followed up.Results The tumor cells in lymph nodes of colorectal cancer were found by CK20 immunohistochemistry and routine hematoxylin-eosin staining separately,but the positive rate had significant difference(P＜0.01).By CK20 immunohistochemistry,the micrometastasis positive rate was found significantly different between the sentinel lymph nodes and all the lymph nodes (P＜0.05).3-year mortality for the patients with lymph node micrometastasis was significantly higher than those without lymph node micrometastasis (P＜0.05).Conclusion The lymph node micrometastasis was an independent risk factor of influencing the survival time of the colorectal cancer patients.Examining the expression of CK20 by immunohistochemistry is an effective way of detecting colorectal cancer lymph node micrometastasis.     

Clinical Significance of Lymph Node Sampling in Endometrial Carcinoma

OBJECTIVE To study the clinical significance of pelvic and para-aortic lymph node sampling in endometrial carcinoma.METHODS Data were analyzed from 311 patients who received surgical treatment in our hospital during the period from January 1995 to December 2002.Among the patients,197 underwent lymph node sampling or lymph-adenectomy.The patients were divided into 2 groups based on the nature of their lymph node dissection,i.e.a)The sampling group included 114 patients with an extrafascial hysterectomy or modified radical hysterectomy plus pelvic or paraaortic lymph node sampling of the abdominal aorta;b)The dis-section group,included 83 patients with a radical or modified radical hyster-ectomy plus systemic pelvic lymph node clearance or paraaortic lymph node dissection of the abdominal aorta.RESULTS The median of the sampling sites for lymph node removal was 5 in the sampling group,and the median of the lymph nodes removed was 15 per case.Lymph node metastasis was found in 8 cases.In the dissection group,the median of the cases for lymph node removal was 8,and the me-dian of the lymph nodes removed was 27 per case.Lymph node metastasis was found in 6 cases.The 5-year survival rates were 90.2% and 90.9% in the 2 groups,respectively.CONCLUSION Lymph node sampling of endometrial cancer is a good way of precisely finding lymphatic metastases,and is suitable for surgical staging without causing immoderate surgical treatment and without affecting the survival rate.     

大分割放疗联合EP方案化疗治疗局限期小细胞肺癌的临床疗效及安全性

Objective To investigate the efficacy and safety of hypofractionated thoracic radiotherapy combined with EP chemotherapy in the treatment of limited-stage small-cell lung cancer (LS-SCLC). Methods A total of 117 patients with LS-SCLC were enrolled and randomly divided into test group (n=59) and control group (n=58). Patients in the experiment group were given hypofractionated thoracic radiotherapy combined with EP chemotherapy, while patients in the control group were given hyperfractionation radiotherapy combined with EP chemotherapy. Objective response rate (ORR), 2-year overall survival (OS), 2-year progression free survival (PFS), and immune cell level were used to evaluate clinical efficacy. We compared the incidence of side effects between the two groups. Results After the treatment, the ORR of patients in the test group was higher than that in the control group (P>0.05). The mean OS and PFS of patients in the test group were significantly longer than those in the control group (P<0.05). The levels of CD3+, CD4+, CD4+/CD8+, and NK cells in the test group were significantly higher, whereas the levels of CD8+ were significantly lower than those in the control group (P<0.05). The incidence of radiation pneumonitis, radiation esophagitis, and severe dermatitis in the test group was significantly lower than that in the control group (P<0.05). Conclusion Hypofractionated radiotherapy combined with EP chemotherapy for treatment of LS-SCLC can effectively improve the anticancer efficacy and patient survival, reduce the damage to the body’s immune function, and alleviate adverse reaction of radiotherapy. © 2023, CHINA RESEARCH ON PREVENTION AND TREATMENT. All rights reserved.     

The Effects of Microwave Ablation and Surgical Resection on Hematogenous Dissemination of Cancer Cells in Treating Patients with Small Primary Hepatocellular Carcinoma

OBJECTIVE To conduct a comparative study of the effects of treatment using microwave ablation versus surgical resection on hematogenous dissemination of cancer cells, and on the level of immune cells of the peripheral blood in patients with small primary hepatocellular carcinoma (PHC,≤5 cm). METHODS Forty patients with small PHC (maximal diameter≤5 cm) were divided into a microwave group (19 cases) and a surgical operation group (21 cases). A real-time (RT) quantitative nested RT-PCR examination was performed for peripheral blood alpha-fetoprotein (AFP) mRNA. Studies were conducted to determine the level of CD3, CD4, CD8 and CD4/CD8 cells and for liver function at 30 min before, and 30 min,1 day and 3 days after the treatment. RESULTS Compared to the value before ablation, no obvious changes of CD3, CD4, CD8 and CD4/CD8 cells were found in patients of the microwave group within 7 days after ablation, but CD3, CD4 and CD4/CD8 cells in the operation group were lower compared to that before operation. The copy number of AFP mRNA in the peripheral blood samples of the patients of the 2 groups before operation was determined in 67.5% of the patients (27/40). There was an rise in the expression after treatment but no statistical difference was found in comparing the 2 groups. Follow-up of the patients was conducted for 1 to 16 months. For patients with continuous expression of peripheral blood AFP mRNA, the possibility of relapse and metastasis was increased. CONCLUSION Surgical resection or microwave ablation can cause more exfoliation of hepatoma carcinoma cells in the peripheral blood of patients with small PHC. The immune function of peripheral blood cells decreased in the patients after surgical resection, however, the immune function was better protected following microwave ablation. Microwave ablation causes minor reduction in liver function, and the treatment method presents a definite value for PHC therapy.     

贲门癌患者外周血CD4+CD25hiCD127low调节性T细胞水平及其临床意义

Objective To analyse the dysfunction of immunity and clinical significance in patients with cardiac cancer.Methods The level of CD4+ CD25hi CD127low Treg cells were detected by flow cytometry (FCM),and serum IL-10 and TGF-β1 levels were determined by enzyme linked immunosorbent assay (ELISA) kit in 56 patients with cardiac cancer.15 healthy volunteers were tested as normal controls.The clinical data of each patient were collected and analyzed. Results There was a significantly higher percentage of CD4+ CD25hi CD127low Treg cells in patients with cardiac cancer (5.73±1.56)% than that (4.45±1.06)% of healthy volunteers (P<0.01).The IL-10 and TGF-β1 levels in the serum of patients with cardiac cancer were also significantly higher than that of healthy volunteers (P<0.05).There was a positive correlation between levels of IL-10.TGF-β1 and the levels of CD4+ CD25hi CD127low Treg cells.The number of CD4+ CD25hi CD127low regulatory T cells in the peripheral blood of cardiac cancer patients were significantly correlated with clinical stages and metastasis lymph node.Conclusion The CD4+ CD25hi CD127low Treg cells in the peripheral blood of cardiac cancer patients is significantly increased in comparison with that in healthy volunteers,and was also correlated with different stages.The abnormal levels of CD4+ CD25hi CD127low Treg cells may be related to tumor progression in patients with cardiac cancer.     

贲门癌患者外周血CD4+CD25hiCD127low调节性T细胞水平及其临床意义

Objective To analyse the dysfunction of immunity and clinical significance in patients with cardiac cancer.Methods The level of CD4+ CD25hi CD127low Treg cells were detected by flow cytometry (FCM),and serum IL-10 and TGF-β1 levels were determined by enzyme linked immunosorbent assay (ELISA) kit in 56 patients with cardiac cancer.15 healthy volunteers were tested as normal controls.The clinical data of each patient were collected and analyzed. Results There was a significantly higher percentage of CD4+ CD25hi CD127low Treg cells in patients with cardiac cancer (5.73±1.56)% than that (4.45±1.06)% of healthy volunteers (P<0.01).The IL-10 and TGF-β1 levels in the serum of patients with cardiac cancer were also significantly higher than that of healthy volunteers (P<0.05).There was a positive correlation between levels of IL-10.TGF-β1 and the levels of CD4+ CD25hi CD127low Treg cells.The number of CD4+ CD25hi CD127low regulatory T cells in the peripheral blood of cardiac cancer patients were significantly correlated with clinical stages and metastasis lymph node.Conclusion The CD4+ CD25hi CD127low Treg cells in the peripheral blood of cardiac cancer patients is significantly increased in comparison with that in healthy volunteers,and was also correlated with different stages.The abnormal levels of CD4+ CD25hi CD127low Treg cells may be related to tumor progression in patients with cardiac cancer.     

CARCINOEMBRYONIC ANTIGEN EXPRESSION IN GASTRIC CANCER AS RELATED TO BIOLOGICAL BEHAVIOUR AND PROGNOSIS

Objective: To determine the relationship betweencarcincembryonic antigen(CEA)expression in gastriccancer and biological behaviour or prognosis.Material and Methods: Surgically resected speci-mens of gastric cancer from 104 patients were obtained.The content and distribution of CEA in gastric cancerwere studied by immunohistochemical staining andimmunoelectron microscopic technique. The relationshipbetween CEA in gastric cancer and biological behaviouror prognosis were evaluated.Results: The positivity of CEA Was significantlyhigher in the patients with advanced stage, vascularinvasion and lymph node metastasis than that in thepatients without. The S-year survival rate of thc CEA (-)group was significantly higher than that of the CEA ( )group. Among the patients with advanced stage orlymph node metastasis, the survival rate was higher in theCEA(-) group tban in the CEA( )group.Conclusions: Immunostaining for CEA in gastriccancer tissue may be helpful in differentiating amongtumors that appear similar by c     

不同淋巴结清扫范围的胃癌根治手术对机体的侵袭

Objective： To evaluate the effects of surgical trauma of open surgery on the patients with gastric carcinoma who underwent different lymph node dissection. Methods： Total 30 patients with gastric carcinoma were divided into three groups （D1, D2, and D3） according to the extent of lymph node dissection. Peripheral blood samples were taken to measure the levels of interleukin-6 （IL-6）, interleukin-8 （IL-8）, C-reactive protein （CRP） and polymorphonuclear elastase （PMNE）. Ad- ditionally, leucocytes and lymphocytes counts in peripheral serum were also detected. Results： All the three groups showed a significant increase of the levels of IL-6, IL-8, CRP and PMNE after operation. There was no significant difference between D1 and D2 groups. When the comparison was made between D3 group and the other two groups, it showed higher concentration of IL-6, IL-8, CRP and PMNE in serum of D3 group. Leucocytes count showed no difference among the three groups. After operation, the patients in three groups had transient lymphocytes decrease on the second and third postoperative days, the lymphocytes count in D3 group was still lower while those in D1 and D2 groups began to increase. Conclusion： IL-6, IL-8, CRP and PMNE can be used to monitor surgical stress. Using these parameters, we found that extended lymph node dissection of D3 group led to more postoperative stress than D1 and D2 groups.     

Expression of sIL-2R before and after chemotherapy in patients with breast cancer

Objective:To investigate the difference of peripheral blood sIL-2R before and after chemotherapy in breast cancer patients,and evaluate the clinical value of the sIL-2R in breast cancer's diagnosis and therapy.Methods:The peripheral blood sIL-2R levels of the breast cancer patients with or without chemotherapy were detected by ELISA.The healthy persons were made as the control group.Results:The slL-2R levels of the breast cancer patients were higher than that of the control group(P＜0.05);the slL-2R's levels in Ⅰ～Ⅱ stage breast cancer were lower than that in Ⅲ～Ⅳ stag e breast cancer (P＜0.05);the sIL-2R levels of the patients before chemotherapy were higher than that of the patients undergone chemotherapy(P＜0.05);The level of the patient with chemotherapy was still higher than that of the control group(P＜0.05);the sIL-2R levels of the patients whose chemotherapies were noneffective were higher than that of the patients received effective chemotherapies(P＜0.05).There was no significant difference between the group with ER( )or PR( )and the group with ER(-)or PR(-)(P＞0.05).Conclusion:The breast cancer patients have the high slL-2R levels.There is a close relationship between the cancer incidence and the patients,immune situation.The level of slL-2R could be a clinical index which Can be used for evaluating the cancer degree,because the higher levels of slL-2R can indicate that the immune ability of patient is worse.There is a significant difference between the slL-2R levels of the patients before chemotherapy and that of the patients undergone chemotherapy.     

老年食管癌患者围术期T淋巴细胞亚群的变化(英文)

Objective:The aim of the study was to observe and compare the trend of T-lymphocyte subsets in the elder and adult esophageal carcinoma patients postoperatively. Methods: Forty-four esophageal carcinoma patients, classified as ASA physical status I-II, were divided into two groups. Twenty-two patients aged than 65 years were grouped as elder patient group, and the rest twenty-two patients, served as adult patient group, were younger than 60 years old. The jugular venous blood samples were collected before i...     

介入热化疗对肝癌患者淋巴细胞免疫功能的影响

目的:探讨肝癌患者经肝动脉热化疗栓塞(热疗组)前后T细胞亚群和NK细胞的变化及其临床意义。方法:采用免疫荧光染色法,检测84例原发性肝癌患者热化疗栓塞前后T细胞亚群及NK细胞的变化,并与78例常规介入化疗栓塞组(非热疗组)进行比较。结果:2组治疗前、后T细胞亚群及NK细胞变化的自身比较结果显示,热疗组CD3 、CD4 、CD8 、CD4 /CD8 下降不明显(P>0.05),而治疗后NK细胞有明显增加(P<0.05);非热疗组CD3 、CD4 、CD4 /CD8 均较治疗前明显下降(P<0.05)。治疗前2组T细胞亚群及NK细胞差别不明显(P>0.05);治疗后2周检测,热疗组CD3 、CD4 、CD4 /CD8 及NK细胞明显高于非热疗组,差异有统计学意义(P<0.05)。结论:介入热化疗能快速增加血液中T淋巴细胞及NK细胞的数量,抵消常规介入引起的免疫功能下降,对控制肿瘤的转移可能起到积极的作用。     

The expression and clinical value of tumor infiltrating dendritic cells in tumor tissues of patients with esophageal cancer

BackgroundAs dendritic cells (DCs) are the major antigen-presenting cells of the immune system, understanding their role in esophageal cancer is essential for the development of preventative and treatment strategies. This study investigated the expression level and clinical value of tumor infiltrating dendritic cells (TIDCs) in tumor tissues of patients with esophageal cancer.MethodsFrom January 2019 to January 2021, 184 patients with esophageal cancer treated were prospectively enrolled as the observation group and 184 patients with benign esophageal tumors were selected as the control group. Tumor tissue samples were obtained and the expression level and phenotypes of the TIDCs were analyzed. The correlation between TIDC expression and clinical characteristics of patients with esophageal cancer was investigated.ResultsThe density of the TIDCs in the observation group was lower than that in the control group (8.76±2.25 vs. 9.97±2.19; P=0.000). Furthermore, the percentage of major histocompatibility complex-II (MHC-II) positive DCs and the percentage of CD54 positive DCs were relatively lower in the observation group compared to the control group (6.60%±2.12% vs. 9.34%±2.41%; P=0.000 and 7.41%±2.36% vs. 9.98%±2.47%; P=0.000, respectively). Esophageal cancer patients with lymph node metastasis had lower TIDC density, lower percentage of MHC-II positive DCs, and lower percentage of CD54 positive DCs compared to patients without node metastasis (P<0.05). Patients with stage III esophageal cancer also showed significantly lower TIDC density, lower percentage of MHC-II positive DCs, and lower percentage of CD54 positive DCs compared to patients with stage I/II esophageal cancer (P<0.05). Esophageal cancer patients with tumor diameter ≥4 cm presented with decreased TIDC density, decreased percentage of MHC-II positive DCs, and decreased percentage of CD54 positive DCs compared to patients with tumor diameter <4 cm (P<0.05). In addition, the density of TIDCs, the percentage of MHC-II positive DCs, and the percentage of CD54 positive DCs were significantly negatively correlated with the percentage of CD4⁺ T-lymphocytes and positively correlated with the percentage of CD8⁺ T-lymphocytes (P<0.05).ConclusionsPatients with esophageal cancer had low expression and function of TIDCs, and this was related to the imbalance of T-lymphocyte subsets, lymph node metastasis, TNM stage, and lesion size.     

非小细胞肺癌组织中T淋巴细胞亚群的观察

目的:了解非小细胞肺癌组织局部免疫功能状况。方法:应用免疫组织化学技术对34例肺鳞癌和腺癌组织中的T淋巴细胞亚群(T—Ls)进行了原位定量观察。结果:肺癌组织比肺良性疾病组织中CD_4~ /CD_8~ 细胞比值降低,差异非常显著(P<0.01);肺癌淋巴结转移组、低分化肺癌组比对照组组织中CD_3~ 细胞减少,差异显著(P<0.05)。结论:肺癌组织局部的免疫功能低下,细胞免疫的调节机能紊乱。肺癌伴有淋巴结转移患者比未转移患者局部的免疫功能更低下。肺癌分化程度越低,局部的免疫机能越差。     

胃癌癌周淋巴结网膜及腹膜中T淋巴细胞亚群和NK细胞分布的研究

目的:通过检测胃癌、癌周正常胃粘膜、胃周淋巴结、大网膜、小网膜、腹膜及外周血中T淋巴细胞亚群和NK细胞数以探讨胃癌局部及全身的免疫状况.方法:利用流式细胞术测定40例胃癌患者的癌组织、癌周正常胃粘膜、胃周淋巴结、大网膜、小网膜、腹膜及外周血的CD4 、CD8 及NK细胞的含量.取22例良性疾病患者的胃粘膜和外周血做对照.结果:CD4 、CD8 、NK细胞计数在胃癌组织和胃周淋巴结中最高,均高于癌周正常胃粘膜、大网膜、小网膜、腹膜及外周血(P＜0.05),但癌组织的CD4 /CD8 比值低于淋巴结和大网膜(P＜0.05);良性疾病对照组的胃粘膜、外周血CD4 、CD8 、NK细胞计数和CD4 /CD8 比值均高于胃癌患者(P＜0.01).结论:胃癌患者局部及全身的免疫功能均低于对照组;胃癌组织内CD4 、CD8 、NK细胞较癌周正常胃粘膜、大网膜、小网膜、腹膜高聚集,但免疫功能呈现低下和抑制状态.     

上皮性卵巢癌患者血清可溶性白细胞介素-2受体、人附睾蛋白4、T淋巴细胞亚群水平及临床意义

目的探讨上皮性卵巢癌患者的血清可溶性白细胞介素-2受体(SIL-2R)、人附睾蛋白4(HE4)、T淋巴细胞亚群水平及临床意义。方法选择100例上皮性卵巢癌患者和100例卵巢良性肿瘤患者,分别作为观察组和对照组,比较两组患者的血清SIL-2R、HE4及T淋巴细胞亚群(CD3⁺、CD4⁺、CD8⁺和CD4⁺/CD8⁺)水平,分析不同临床特征上皮性卵巢癌患者的血清SIL-2R、HE4及T淋巴细胞亚群水平。结果观察组患者的血清SIL-2R、HE4水平均明显高于对照组,差异均有统计学意义(P﹤0.01)。观察组患者的血清CD3⁺、CD4⁺水平及CD4⁺/CD8⁺均明显低于对照组,CD8⁺水平明显高于对照组,差异均有统计学意义(P﹤0.01)。临床分期为Ⅲ~Ⅳ期、中/低分化、有腹腔积液的上皮性卵巢癌患者的血清SIL-2R、HE4水平分别高于临床分期为Ⅰ~Ⅱ期、高分化、无腹腔积液的患者,差异均有统计学意义(P﹤0.01)。中/低分化、有淋巴结转移、有腹腔积液上皮性卵巢癌患者的血清CD3⁺、CD4⁺水平分别低于高分化、无淋巴结转移、无腹腔积液的患者,CD8⁺水平分别高于高分化、无淋巴结转移、无腹腔积液的患者,差异均有统计学意义(P﹤0.05)。上皮性卵巢癌患者的血清SIL-2R、HE4水平与CD3⁺、CD4⁺、CD4⁺/CD8⁺均呈负相关(P﹤0.05),与CD8⁺呈正相关(P﹤0.05)。结论上皮性卵巢癌患者血清中SIL-2R、HE4水平均较高,T淋巴细胞亚群CD3⁺、CD4⁺水平及CD4⁺/CD8⁺均较低,SIL-2R、HE4水平与T淋巴细胞亚群之间具有相关性。     

CD95、CD44V6与乳腺癌淋巴结微小转移的关系

目的探讨乳腺癌组织中CD95、CD44V6的表达与腋窝淋巴结转移的关系及其临床病理意义。方法筛选101例乳腺癌根治标本,癌组织免疫组织化学标记CD95与CD44V6其中40例腋窝淋巴结阴性者淋巴结全部标记MCKo并进行统计学分析。结果在常规病理诊断淋巴结未转移的病例中,免疫组化标记检出9例（22．5％,9／40）有微小转移。CD95与CD44V6的表达在转移组与微转移组相似。淋巴结转移组CD95的阳性率与未转移组之间差异具有统计学意义。当肿瘤〉2cm时,CD95的阳性率及CD44V6的高表达率具有统计学意义（P〈0．05）。结论通过检测乳腺癌组织中CD95与CD44V6的表达,有助于预测淋巴结微小转移情况,为临床判断预后和选择治疗方案提供更加可靠的依据。     

老年食管癌患者围术期Ｔ淋巴细胞亚群的变化

目的：观察比较老年组和成年组食管癌患者术中、术后Ｔ淋巴细胞亚群变化的趋势。方法：４４例ＡＳＡⅠ ～Ⅱ级食管癌患者分为两组,其中２２例年龄６５岁以上的为老年组;２２例年龄６０岁以下的为成年组。分别于麻醉前、术毕、术后ｄ_１、术后ｄ_２、术后ｄ_５共５个时间点抽取静脉血;采用流式细胞仪测定各组外周血Ｔ淋巴细胞亚群（ＣＤ３＋、ＣＤ４＋、ＣＤ８＋）。结果：术前老年组与成年组之间患者外周血ＣＤ３＋、ＣＤ４＋、ＣＤ４／ＣＤ８水平之间无显著性差异,与各自的麻醉前值相比较,术毕、术后ｄ１、术后ｄ２,两组患者外周血的ＣＤ３＋、ＣＤ４＋及ＣＤ４／ＣＤ８均明显下降（Ｐ＜０.０１）;术后ｄ５成年组的ＣＤ４／ＣＤ８恢复到麻醉前水平（Ｐ＞０.０５）,并明显高于老年组（Ｐ＜０.０５）。结论：全麻开胸术后,食管癌患者均有不同程度细胞免疫抑制现象,老年组Ｔ细胞亚群恢复至正常的时间慢于成年组患者。     

喉咽鳞癌患者外周血T淋巴细胞亚群和NK细胞活性分析

背景与目的:T淋巴细胞亚群及NK细胞是主要的细胞免疫形式,通过对两者的研究可以了解细胞免疫在肿瘤发生、发展的作用。喉咽鳞癌患者可能存在一定程度的细胞免疫缺陷,本研究是通过检测外周血T淋巴细胞亚群及NK细胞活性,了解喉咽鳞癌患者的细胞免疫功能。方法:用流式细胞仪对78例喉咽鳞癌患者的外周血进行T淋巴细胞亚群及NK细胞活性的检测,并取20例非肿瘤患者的外周血作为对照组。结果:喉咽鳞癌患者组较非肿瘤患者组CD4淋巴亚群、CD4/CD8比值下降,CD8淋巴亚群升高;NK细胞活性下降。T3～4组较T1～2组、N 组较N0组都有CD4淋巴亚群、CD4/CD8比值下降;NK细胞活性下降。中低分化组CD4淋巴亚群、CD4/CD8比值下降(均P<0.05)。结论:喉咽鳞癌患者CD4T淋巴细胞亚群及NK细胞活性受到抑制,细胞免疫功能降低;检查外周血T淋巴细胞亚群及NK细胞活性有助于喉咽鳞癌患者的细胞免疫功能监测。     

肾移植术后泌尿系恶性肿瘤患者T淋巴细胞亚群的测定及意义

  目的  观察肾移植术后并发泌尿系统恶性肿瘤患者血液T淋巴细胞亚群的变化, 及其对调整免疫抑制剂及预防恶性肿瘤的意义。  方法  9例肾移植术后并发泌尿系统恶性肿瘤患者为肿瘤组, 9例未发生任何肿瘤或感染的肾移植患者为移植组, 9例健康体检者为对照组。流式细胞术测定T淋巴细胞亚群(CD3+T、CD3+CD8+T、CD3+CD4+T、CD4+/CD8+)比例, 肿瘤组行肿瘤切除并减少免疫抑制剂用量, 测定术前及术后2个月血药CsA和FK506浓度。  结果  肿瘤组与另两组比较, CD3+T及CD3+CD8+T无明显变化, CD3+CD4+T明显减少, CD4+/CD8+比值明显降低(P < 0.05)。肿瘤组肿瘤切除术后2个月与术前比较, CD3+T和CD3+CD4+T无明显变化; CD3+CD8+T明显降低, 而CD4+/CD8+比值明显升高(P < 0.05), CsA和FK506血药浓度均明显降低(P < 0.05)。  结论  血T淋巴细胞亚群可作为肾移植免疫状态的有效评价指标, 对指导肾移植免疫抑制剂的调整和预防肿瘤的发生具有重要的临床意义。