分光光度法在血清精氨酸酶活性检测中的应用

目的:建立一种检测血清精氨酸酶活性的快速比色方法。方法:利用对硝基苯乙醛(PNPG)能特异地与精氨酸反应,并在480nm处有最大吸收,同时根据水解后剩余精氨酸的含量来推算精氨酸活性水平。结果:通过精密实验可得批内CV为3.8%,批间CV为4.7%。并测定32份正常人血清,男性0～75(44±10)U/L,女性0～79(44±10)U/L,男女间无显著差异(P>0.05)。结论:该方法特异、准确,操作简捷,灵敏度高,适宜于临床实验室中广泛开展。     

人血清中视黄醇结合蛋白的激光散射比浊测定及临床应用

本文介绍了血清视黄醇结合蛋白(RBP)的激光散射比浊测定法。此法操作简单、快速、且能自动化。视黄醇结合蛋白浓度在9.0～146.0mg/L之间呈线性;批内CV9.1％;批间CV11.6％;回收率为97.5～109.0％。测定41名健康成人正常参考值为47.6±9.0mg/L(X±SD)。肝脏损害患者血清RBP为18.6±11.0mg/L,明显低于正常人。而肾脏损害患血清RBP为244.5±15.2mg/L,明显高于正常人。     

马贡法测定血清镁的方法学探讨

本文介绍一种简单、灵敏的血清镁马贡(Magon)测定法。血清镁在0～4mmol/L内线性良好。平均回收率101.9％。与MTB法比较r=0.9913,=0.992x+0.019,批内CV3.92％,批间CV4.27％。98例健康人血清镁测定结果为0.96±0.065mmol/L,干扰实验:Zn~(++)60μmol/L、K~+50mmol/L、Na~+、Cl-560mmol、Ca~(++)10mmol/L,甘油三酯9.67mmol/L对结果无影响。     

人碱性谷胱甘肽S-转移酶放射免疫分析

用人肝碱性谷胱甘肽S-转移酶及其抗血清,建立了血清碱性谷胱甘肽放射免疫分析,该法灵敏度为0.85μg/L,回收率为95～100％,线性相关系数为0.999,批出CV为7.9％(n=8),批间CV为8.3％(n=5),NSB为2.20～4.55％,在1个月内稳定。70名正常成人血清碱性谷胱甘肽均值为3.14±1.99μg/L,正常值为0～7.09μg/L。本法为开展血清碱性谷胱甘肽的基础和临床研究提供了有效的手段和正常值参数。     

TBARS比色测定血浆低密度脂蛋白的研究

目的 :建立和改良血浆低密度脂蛋白 (LDL)直接测定法。方法 :TBARS比色法。结果 :该方法线性范围 0～ 10mmol/L ,与酶法相关 r=0 .985 3 ,批内CV =1.5 1% ,批内CV =2 .84% ,平均回收率 10 0 .4%。结论 :该法结果准确可靠 ,灵敏度高 ,测定范围大 ,适于临床应用。LDL C正常参考值 18～ 42岁为 2 .5 3± 0 .82mmol/L ;43～ 63岁为 3 .0 1± 1.2mmol/L。     

血清白细胞弹性蛋白酶的检测及其在急性胰腺炎病情观察中的应用

为建立酶动力学方法检测血清白细胞弹性蛋白酶(Human leukocyte elas-tase,HLE),选择高敏感底物琥珀酰-(L-丙氨酸)2-缬氨酸-对硝基苯胺。该底物在HLE催化下释放出对硝基苯胺。通过在405nm处测定产物吸光度的变化,推算出HLE的活性。结果显示,该实验的米氏常数为0.086mmol/L,最适底物浓度为2.0mmol/L,最适PH为8.0,线性范围O～300U/L;精密度试验高、中、低值分别为批内CV(％)1.25、2.60、4.80,批间CV(％)1.77、3.50、5.15;正常参考值为8～20U/L;轻、重两型胰腺炎患者血清HLE与正常对照组均有显著性差异。提示该法简捷、快速、准确、灵敏,可用于手工操作及自动化分析。     

组织多肽特异性抗原水平检测对良恶性胸水的诊断价值

目的:探讨血清及胸水中组织多肽特异性抗原(TPS)对良恶性胸水的诊断价值。方法:用酶联免疫吸附分析法(ELISA)测定了32例肺癌患者(肺癌组)和30例结核性胸膜炎患者(对照组)血清及胸水中TPS的水平。结果:肺癌组血清中TPS水平为271.37±94.96U/L,显著高于良性对照组(145.06±77.02U/L)。肺癌组胸水中TPS水平为406.85±103.43U/L,显著高于良性对照组(206.50±157.37U/L)。非小细胞癌(NSCLC)血清TPS水平为302.49±76.09U/L,显著高于小细胞癌(SCLG)组(157.24±66.92U/L);鳞癌血清TPS水平为338.13±82.70U/L,显著高于腺癌组(272.80±57.81U/L)。NSCLC的胸水TPS水平为469.56±81.76U/L,显著高于SCLC组(273.65±123.36U/L),两组比较有极显著性差异(P<0.01)。虽然鳞癌胸水TPS水平(469.56±81.76U/L)高于腺癌胸水TPS水平(441.13±54.46U/L),但无显著性差异(P>0.05)。结论:TPS水平检测对良恶性胸水的鉴别诊断具有重要价值,同时也是鉴别小细胞肺癌和非小细胞肺癌的较好指标之一。     

高铁血红蛋白的测定在肝病中的意义

目的:探讨高铁血红蛋白的测定在肝病中的意义。方法:用高铁血红蛋白定量法,对90例病毒性肝炎和10例原发性肝癌(HBsAg+)病人检测高铁血红蛋白含量,其中急性肝炎(ALT>800u/L)54例,HBsAg+(ALT<60U/L)12例,慢性肝炎(ALT 200～60u/L)12例,慢性肝炎(ALT>200u/L)12例,原发性肝癌(HBsAg+,其中3例ALT<60U/L,7例ALT>100U/L)10例。结果:高铁血红蛋白血中的浓度在急性肝炎、慢性肝炎(ALT>200U/L)、原发性肝癌三组中X±s分别为2.07±2.00,4.45±4.00,6.07±4.99g/L,均显著高于正常对照组(0.72±0.59g/L)(P<0.05～P<0.001),而HBsAg+(ALT<60U/L),慢性肝炎(ALT200～60U/L)两组中X±s分别为0.52±0.44,1.55±0.65g/L,与正常对照组比较无显著性差异(P>0.05)。结论:高铁血红蛋白含量与肝功能有关,与肝癌有关。     

原发性肝癌血清CA_(125)的检测

目的:探讨血清CA_(125)对肝癌的诊断价值。方法:采用ELISA法,对肝癌患者44例,肝硬化60例,脂肪肝70例、慢性肝炎75例及35例正常对照进行CA_(125)测定。结果:肝癌患者血清CA_(125)含量为(24.5±12.1)万U/L,显著高于肝硬化组、脂肪肝组、慢性肝炎组和正常对照组,分别为[(3.25±1.25)万U/L,(2.48±0.92)万U/L,(2.33±0.85)万U/L,(2.08±0.79)万 U/L,P<0.01)],肝硬化组高于脂肪肝组、慢性肝炎组和正常对照组(P<0.05),但显著低于肝癌组(P相似文献   

人血清免疫反应性胰蛋白酶快速RIA及对急性胰腺炎的诊断作用

人血清免疫反应性胰蛋白酶(IRT)快速RIA能在2h内完成。该方法灵敏度为1.5ng/ml,批内CV为4.0％,批间为7.2％,回收率103％,健全性呈线性关系,稳定性能保持2个月。国人血清IRT正常值(X±SD)为30.2±12.3ng/ml(12～70ng/ml,n=120),22例急性胰腺炎病人的血清IRT显著增高(793.9±618.8     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.