Na+-activated K+ current in cardiac cells: rectification,open probability,block and role in digitalis toxicity

The Na⁺-activated K⁺ current was studied in inside-out patches and in whole cells isolated from the guinea-pig cardiac ventricle. The single channel conductance showed inward rectification for K⁺ _i+ _e, but outward rectification for K⁺ _i>K⁺ _e The open probability was dependent on Na⁺ _i and Na⁺,K⁺-pump activity. In the presence of pump blockade the channel remained active at low Na⁺ _i Similar results were obtained in whole cells. These results suggest the existence of Na⁺ gradients depending on Na⁺,K⁺-pump activity and passive inward leak of Na⁺. The channel and whole cell current were blocked by R56865. The drug did not change the single channel conductance but markedly reduced open probability by shortening burst duration. The current may play an important role in action potential shortening during pump blockade.This work was supported by a grant of the National Fund for Scientific Research Belgium.3.0016.87.     

Computer simulation of wild-type and mutant human cardiac Na+ current

Long QT syndrome (LQTS) and Brugada syndrome (BrS) are inherited diseases predisposing to ventricular arrhythmias and sudden death. Genetic studies linked LQTS and BrS to mutations in genes encoding for cardiac ion channels. Recently, two novel missense mutations at the same codon in the gene encoding the cardiac Na⁺ channel (SCN5A) have been identified: Y1795C (causing the LQTS phenotype) and Y1795H (causing the BrS phenotype). Functional studies in HEK293 cells showed that both mutations alter the inactivation of Na⁺ current and cause a sustained Na⁺ current upon depolarisation. In this paper, a nine state Markov model was used to simulate the Na⁺ current in wild-type Na⁺ cardiac channel and the current alterations observed in Y1795C and Y1795H mutant channels. The model includes three distinct closed states, a conducting open state and five inactivation states (one fast-, two intermediate- and two closed-inactivation). Transition rates between these states were identified on the basis of previously published voltage-clamp experiments. The model was able to reproduce the experimental Na⁺ current in mutant channels just by altering the assignment of model parameters with respect to wild-type case. Parameter assignment was validated by performing action potential clamp experiments and comparing experimental and simulated I _Na current. The Markov model was subsequently introduced in the Luo–Rudy model of ventricular myocyte to investigate “in silico” the consequences on the ventricular cell action potential of the two mutations. Coherently with their phenotypes, the Y1795C mutation prolongs the action potential, while the Y1795H mutation causes only negligible changes in action potential morphology.     

Intracellular Na+ modulates large conductance Ca2+-activated K+ currents in human umbilical vein endothelial cells

We studied the effects of Na⁺ influx on large-conductance Ca²⁺-activated K⁺ (BK_Ca) channels in cultured human umbilical vein endothelial cells (HUVECs) by means of patch clamp and SBFI microfluorescence measurements. In current-clamped HUVECs, extracellular Na⁺ replacement by NMDG⁺ or mannitol hyperpolarized cells. In voltage-clamped HUVECs, changing membrane potential from 0 mV to negative potentials increased intracellular Na⁺ concentration ([Na⁺]_i) and vice versa. In addition, extracellular Na⁺ depletion decreased [Na⁺]_i. In voltage-clamped cells, BK_Ca currents were markedly increased by extracellular Na⁺ depletion. In inside-out patches, increasing [Na⁺]_i from 0 to 20 or 40 mM reduced single channel conductance but not open probability (NPo) of BK_Ca channels and decreasing intracellular K⁺ concentration ([K⁺]_i) gradually from 140 to 70 mM reduced both single channel conductance and NPo. Furthermore, increasing [Na⁺]_i gradually from 0 to 70 mM, by replacing K⁺, markedly reduced single channel conductance and NPo. The Na⁺–Ca²⁺ exchange blocker Ni²⁺ or KB-R7943 decreased [Na⁺]_i and increased BK_Ca currents simultaneously, and the Na⁺ ionophore monensin completely inhibited BK_Ca currents. BK_Ca currents were significantly augmented by increasing extracellular K⁺ concentration ([K⁺]_o) from 6 to 12 mM and significantly reduced by decreasing [K⁺]_o from 12 or 6 to 0 mM or applying the Na⁺–K⁺ pump inhibitor ouabain. These results suggest that intracellular Na⁺ inhibit single channel conductance of BK_Ca channels and that intracellular K⁺ increases single channel conductance and NPo. GH Liang and MY Kim contributed equally to this publication and therefore share the first authorship.     

Single calcium-activated potassium channel in cultured mammary epithelial cells

The properties of Ca²⁺-activated K⁺ channels in mouse mammary epithelial cells in primary culture were studied by the patch-clamp technique. In cell-attached patches, spontaneous channel openings were sometimes observed; the slope conductance of the currents was about served; the slope conductance of the currents was about 12 pS at negative membrane potentials with a physiological solution (152 mM Na⁺, 5.4 mM K⁺) in the pipette. External application of A23187, a calcium ionophore, activated this channel. In excised inside-out patches, the channel was activated by increasing the internal Ca²⁺ concentration (10^–7 to 10^–6 M). No voltage dependence of the channel activity was observed. Internal Na⁺ blocked the outward K⁺ current in a voltage dependent manner and this block led to the non-linear I–V relationship at positive membrane potentials. The channel was blocked by internal Ba²⁺ (0.1 mM) and tetracthylammonium (TEA⁺, 20–50 mM). Ba²⁺ reduced the open probability but not the single channel conductance, whereas TEA⁺ reduced the single channel conductance. The single channel conductance of this channel, measured from the inward current with a high-K⁺ solution (150 mM K⁺) in the pipette, was large (about 40 pS), and showed inward rectification. These results suggest that this channel is different from the usual small conductance Ca²⁺-activated K⁺ channels observed in many other cells.     

Multiple types of Na+ currents mediate action potential electrogenesis in small neurons of mouse dorsal root ganglia

Small (<25 μm in diameter) neurons of the dorsal root ganglion (DRG) express multiple voltage-gated Na⁺ channel subtypes, two of which being resistant to tetrodotoxin (TTX). Each subtype mediates Na⁺ current with distinct kinetic property. However, it is not known how each type of Na⁺ channel contributes to the generation of action potentials in small DRG neurons. Therefore, we investigated the correlation between Na⁺ currents in voltage-clamp recordings and corresponding action potentials in current-clamp recordings, using wild-type (WT) and Na_V1.8 knock-out (KO) mice, to clarify the action potential electrogenesis in small DRG neurons. We classified Na⁺ currents in small DRG neurons into three categories on the basis of TTX sensitivity and kinetic properties, i.e., TTX-sensitive (TTX-S)/fast Na⁺ current, TTX-resistant (TTX-R)/slow Na⁺ current, and TTX-R/persistent Na⁺ current. Our concurrent voltage- and current-clamp recordings from the same neuron revealed that the action potentials in WT small DRG neurons were mainly dependent on TTX-R/slow Na⁺ current mediated by Na_V1.8. It was surprising that a large portion of TTX-S/fast Na⁺ current was switched off in WT small DRG neurons due to a hyperpolarizing shift of the steady-state inactivation (h _∞), whereas in KO small DRG neurons which are devoid of TTX-R/slow Na⁺ current, the action potentials were generated by TTX-S/fast Na⁺ current possibly through a compensatory shift of h _∞ in the positive direction. We also confirmed that TTX-R/persistent Na⁺ current mediated by Na_V1.9 actually regulates subthreshold excitability in small DRG neurons. In addition, we demon strated that TTX-R/persistent Na⁺ current can carry an action potential when the amplitude of this current was abnormally increased. Thus, our results indicate that the action potentials in small DRG neurons are generated and regulated with a combination of multiple mechanisms that may give rise to unique functional properties of small DRG neurons.     

Novel Brugada syndrome‐causing mutation in ion‐conducting pore of cardiac Na+ channel does not affect ion selectivity properties

Aim: Brugada syndrome is an inherited cardiac disease with an increased risk of sudden cardiac death. Thus far Brugada syndrome has been linked only to mutations in SCN5A, the gene encoding the α‐subunit of cardiac Na⁺ channel. In this study, a novel SCN5A gene mutation (D1714G) is reported, which has been found in a 57‐year‐old male patient. Since the mutation is located in a segment of the ion‐conducting pore of the cardiac Na⁺ channel, which putatively determines ion selectivity, it may affect ion selectivity properties. Methods: HEK‐293 cells were transfected with wild‐type (WT) or D1714G α‐subunit and β‐subunit cDNA. Whole‐cell configuration of the patch‐clamp technique was used to study biophysical properties at room temperature (21 °C) and physiological temperature (36 °C). This study represents the first measurements of human Na⁺ channel kinetics at 36 °C. Ion selectivity, current density, and gating properties of WT and D1714G channel were studied. Results: D1714G channel yielded nearly 80% reduction of Na⁺ current density at 21 and 36 °C. At both temperatures, no significant changes were observed in V_1/2 values and slope factors for voltage‐dependent activation and inactivation. At 36 °C, but not at 21 °C, D1714G channel exhibited more slow inactivation compared with WT channel. Ion selectivity properties were not affected by the mutation at both temperatures, as assessed by either current or permeability ratio. Conclusion: This study shows no changes in ion selectivity properties of D1714G channel. However, the profoundly decreased current density associated with the D1714G mutation may explain the Brugada syndrome phenotype in our patient.     

Patch-clamp study of rubidium and potassium conductances in single cation channels from mammalian exocrine acini

Single-channel current recordings were carried out on excised inside-out patches of baso-lateral plasma membrane from exocrine acinar cells. The mouse pancreas and submandibular gland as well as the pig pancreas were investigated.In the mouse pancreas the voltage-insensitive Ca²⁺-activated cation channel was studied. Single-channel current-voltage (i/v) relationships were studied in symmetrical Rb⁺-rich solutions and in asymmetrical Rb⁺/Na⁺ and Na⁺/Rb⁺ solutions. In all cases the i/v relations were linear and had the same slope representing a single-channel conductance of about 33 pS which is identical to that previously obtained with symmetrical Na⁺ solutions or asymmetrical Na⁺/K⁺ solutions.In the mouse submandibular gland and the pig pancreas the voltage and Ca²⁺-activated K⁺ channel was studied. The outward currents observed after depolarization in the presence of quasi-physiological Na⁺/K⁺ gradients were immediately abolished when all the K⁺ in the bath fluid was replaced by Rb⁺ (bath fluid in contact with inside of plasma membrane). This effect was immediately and fully reversible upon return to the high K⁺ solution.The voltage and Ca²⁺-activated K⁺ channel was also studied in asymmetrical K⁺/Rb⁺ and Rb⁺/K⁺ solutions. In the first case inward (K⁺) currents could be observed but not outward (Rb⁺) currents, while in the other case inward (Rb⁺) currents could not be seen whereas outward (K⁺) currents were measured. The current-voltage relationships were approximately linear and the null potential was close to 0 mV in both situations. In contrast the null potential for current through the K⁺ channel in the presence of asymmetrical Na⁺/K⁺ or Li⁺/K⁺ solutions was about –70 mV and with reversed gradients about +60 mV.Outward K⁺ currents of reduced size (through the voltage and Ca²⁺-activated K⁺ channel) could be observed when the bath fluid contained 75 mM K⁺ and 75 mM Rb⁺, but not (in the same membrane patches) when 150 mM Rb⁺ and no K⁺ was present.It is concluded that the large voltage- and Ca²⁺-activated K⁺ channel has an extremely low Rb⁺ conductance. It is possible, however, that the permeability for Rb⁺ may be about the same as for K⁺. The voltage-insensitive Ca²⁺-activated cation channel does not discriminate between K⁺ and Rb⁺.     

Interaction between external Na+ and mexiletine on Na+ channel in guinea-pig ventricular myocytes

To assess the modulation of Na⁺ channel block with local anaesthetics by the change of external Na⁺ concentration ([Na⁺]_o), we examined the block by mexiletine at different [Na⁺]_o using the whole-cell and the cell-attached configurations of the patch-clamp technique. Lowering [Na⁺]_o increased the degree of use dependent block of the whole-cell Na⁺ current. The external Na⁺ dependence of the Na⁺ current block was caused by the interaction of mexiletine with the activated Na⁺ channel, but not with the inactivated channel. In single-Na⁺ channel current recordings at a reduced [Na⁺]_o of 70 mM, mexiletine shortened the mean open time of the channels (1.32±0.06 ms in the control vs. 0.86±0.12 ms with the drug, P<0.05) without changes in the unitary current amplitude, whereas the drug did not affect mean open time at a [Na⁺]_o of 140 mM. Moreover, the open time distributions during drug exposure at the reduced [Na⁺]_o were better fitted to a double exponential than to a single exponential in four out of six experiments. These data suggest that mexiletine induces two conductive states: the native open state and a state representing the first step of open channel block. The transition from the former to the latter is dependent on [Na⁺]_o, suggesting an antagonistic interaction of external Na⁺ with mexiletine.     

Fast Na+ current in circular smooth muscle cells of the large intestine

Whole-cell voltage clamp was carried out on freshly dispersed single smooth muscle cells from adult rat and human colons to investigate the regulation of the Ca²⁺ channels. In this study, we unexpectedly discovered the existence of a fast Na⁺ channel current. With normal physiological salt solution (PSS) plus 4-amino-pyridine (3 mM) in the bath and high-Cs⁺ solution in the pipette to inhibit outward K⁺ currents, an inward current possessing fast and slow components was observed when the cell membrane was depolarized to a value more positive than –20 mV from a holding potential of –100 mV. When Ca²⁺ ions were removed from the PSS, or when nifedipine (10 M) and Ni²⁺ (30 M) were simultaneously applied, the slow component disappeared and the fast component remained. The fast current component became almost completely inactivated within 10 ms. This fast component was dependent on extracellular Na⁺ concentration and was inhibited by tetrodotoxin (TTX) dose dependently (IC₅₀ of 130 nM in rat and 14 nM in human). These results suggest that the slow component of inward current was a Ca²⁺ channel current, whereas the fast component was a TTX-sensitive fast Na⁺ channel current. The threshold voltage, the voltage for peak current, and the reversal potential for the fast Na⁺ current were, respectively, about –50, –20, and + 50 mV in rats, and –40, 0, and + 60 mV in humans. The incidence of cells possessing fast Na⁺ currents depended on the region of the colon. In rat proximal colon, the incidence was 64% (14 out of 22 cells tested); in distal colon, it was 10% (2 out of 21 cells tested). In humans, the incidence in the ascending colon was 73% (16 out of 22 cells tested), and in the descending colon was 22% (7 out of 32 cells tested). The densities of fast Na⁺ and Ca²⁺ currents were 3.2 and 4.5 pA/pF in rats and 1.0 and 1.4 pA/pF in humans, respectively. The ratio of both current densities (Na⁺ vs Ca²⁺) was 0.71, in both rats and humans. We conclude that the major ion channels associated with the generation of inward currents in the circular smooth muscle cells of rat and human colon are voltage-dependent Ca²⁺ channels and TTX-sensitive Na⁺ channels. The fast Na⁺ current may facilitate propagation of excitation.     

Tetrodotoxin-sensitive inactivation-resistant sodium channels in pacemaker cells influence heart rate

There is currently some uncertainty about whether cardiac pacemaker cells contain tetrodotoxin (TTX)-sensitive Na⁺ channels although TTX is known to slow heart rate. We have recorded transient and persistent single-channel currents activated by depolarization in myocytes isolated from the toad sinus venosus. The myocytes were identified as pacemaker cells by their characteristic morphology, spontaneous action potentials that were blocked by cobalt but not by TTX, and lack of an inwardly rectifying K⁺ current. The voltage dependence of the single-channel currents, their presence in solutions containing no K⁺ or Ca²⁺, or in solutions to which Cs⁺ and Co²⁺ had been added, their dependence on [Na⁺] and their sensitivity to TTX indicated that they were Na⁺ channel currents. The persistent Na⁺ channel currents were resistant to inactivation and were activated over the range of potentials that occur during diastole in pacemaker cells: they would therefore contribute to the pacemaker current that sets heart rate. It was concluded that TTX slows heart rate by blocking these channels in pacemaker cells. Received: 1 September 1995/Received after revision: 7 November 1995/Accepted: 13 November 1995     

Diacylglycerol-induced activation of protein kinase C attenuates Na+ currents by enhancing inactivation from the closed state

The causes of attenuation of Na⁺ currents by diacylglycerol (DAG)-induced protein kinase C (PKC) activation in mouse neuroblastoma N1E-115 cells were investigated using the cell-attached patch, and the perforated-patch (nystatin based) whole-cell voltage-clamp techniques. Activation of PKC by DAG attenuated Na⁺ currents. Attenuation occurred in the absence of significant changes in the time-course of Na⁺ currents. However, the steady-state inactivation curve of these currents shifted to more negative voltages by approximately 20 mV. Here we demonstrate that the time-course of inactivation is accelerated by treatment with DAG-like substances in a voltage-dependent manner (time constant of inactivation decreased by 2- and 3.6-fold at –60, and –30 mV, respectively). In cell-attached patches, treatment with DAG compounds increased the percentage of current traces showing no single Na⁺ channel openings in response to depolarizing voltage-clamp pulses. Moreover, the average of current traces containing single Na⁺ channel openings was essentially the same in control conditions and after treatment with DAG compounds. Removal of Na⁺ channel inactivation by the alkaloid batrachotoxin prevented the attenuation of Na⁺ currents by PKC activation via DAGs. Taken together, these data strongly suggest that PKC-induced attenuation of Na⁺ currents is linked to an enhancement of Na⁺ channel inactivation. This attenuation is caused by an increase in the number of Na⁺ channels inactivating directly from the closed state(s). This inactivation pathway represents a simple and efficient physiological mechanism by which PKC activation might modulate the electrical activity of excitable cells.     

Inactivation modifiers of Na+ currents and the gating of rat brain Na+ channels in planar lipid membranes

Rat brain Na⁺ channels whose inactivation process had been removed either by batrachotoxin (BTX) or veratridine (VT) were reconstituted into planar lipid membranes. The voltage dependence of the open probability (P _o) of the channel, of the opening and closing rate constants, and the conductance and relative permeability for Na⁺ and K⁺ were studied in voltage-clamp conditions in the presence of agents known to modify the inactivation of Na⁺ currents. In relation to alkaloids (BTX, VT, and aconitine), it was found that once a Na⁺ channel was modified by BTX or VT, the addition of another alkaloid did not change further the gating and permeation properties of the channel over a period of about 1 h. Once the inactivation process of the channels is removed by BTX, the addition of a proteolytic enzyme (trypsin) or an halogenated compound (chloramine-T, CT) induced profound and specific modifications on the opening and closing events of Na⁺ channels: (1) the voltage dependence of the channel P _o shifted to more hyperpolarized potentials; (2) this voltage shift can be explained by equal hyperpolarizing voltage shifts of the opening and closing rate constants of the channel; (3) although the gating properties of the channel were modified by these compounds, the permeation properties of the channel, as evaluated by the conductance and the selectivity to Na⁺ and K⁺ ions, were unaltered; (4) trypsin and CT were active only in the intracellular side of the channel and were irreversible within the time course of the experiments, suggesting covalent modifications of the channel. Inactivation modifiers also affected the gating of toxin-activated single Na⁺ channels. This alteration is compatible with a simple increase in the intracellular potential as seen by the voltage sensor of the channel.     

Effects of veratridine on single neuronal sodium channels expressed inXenopus oocytes

(1) Chick neuronal Na⁺ channels were expressed inXenopus laevis oocytes after injection with total messenger ribonucleic acid (mRNA) isolated from chick brain. The currents were investigated with the whole cell voltage clamp and with the patch clamp technique. Activation and inactivation of the induced current, and its sensitivity towards tetrodotoxin (TTX) and veratridine were reminiscent of vertebrate neuronal Na⁺ channels. (2) In the presence of veratridine normal single channel openings often converted into small amplitude openings of long duration. These small amplitude openings persisted for hundreds of milliseconds after return to the holding potential. (3) The slope conductance of the veratridine modified open channel state was 5–6 pS as compared to the normal state with 21–25 pS in the voltage range between –35 and +5 mV. (4) The modified channel showed saturation behaviour towards Na⁺ ions. Half saturation of the single channel amplitude was observed at 330 mM Na⁺ at a membrane potential of –100 mV. (5) Final closure of the modified channel after return to the holding potential followed an exponential time course. Its potential dependence was similar to that of the time course of the veratridine induced tail currents in the whole cell configuration. (6) The properties of the Na⁺ channel derived from chick forebrain are compared with the properties of the same channel derived from chick skeletal muscle. Both were expressed in the same membrane environment, theXenopus oocyte plasma membrane. While earlier results with Na⁺ channels of muscle origin showed two channel populations, one with short and another with long mean open times, Na⁺ channels of neuronal origin were homogeneous and characterized by short open times.     

Anemonia sulcata toxins modify activation and inactivation of Na+ currents in a crayfish neurone

1. The effects of three toxins (ATX I, II, III) isolated from the sea anemoneAnemonia sulcata were studied in the soma membrane of a crustacean neurone under voltage-clamp conditions. 2. All three toxins affected the action potentials and the Na⁺ currents in a similar manner. The lowest concentrations tested (10 nM, 20 nM and 50 nM for AtX I, II and III, respectively) had pronounced selective effects on the Na⁺ current. No effect on K⁺ or Ca²⁺ currents was observed with concentrations up to 5 M. 3. In the presence of ATX the Na⁺ inactivation was incomplete even with pulses of 700 ms length or strong depolarizing prepulses. 4. Besides the effects on the inactivation process ATX affected also the activation of the Na⁺ current. 5. In cells treated with ATX the negative resistance branch of the peak Na⁺ current voltage relation was shifted by –5 mV to –20 mV. 6. The time to peak was increased for small depolarizations (up to –30 mV) and the rate of rise (I/t) was enlarged by ATX. A slow activating current component was also observed after depolarizing prepulses or if the Na⁺ current was outward. 7. The decay of the Na⁺ tail currents was considerably prolonged after the application of ATX if the membrane was repolarized to potentials more positive than about –60 mV. 8. Repetitive stimulation led to a shortening of the action potential in ATX II treated neurones. A simultaneous and parallel decrement of the peak and plateau current was observed with depolarizing voltage steps.     

Electrogenic Na+/K+-transport in human endothelial cells

Na⁺/K⁺ pump currents were measured in endothelial cells from human umbilical cord vein using the whole-cell or nystatin-perforated-patch-clamp technique combined with intracellular calcium concentration ([Ca²⁺]_i) measurements with Fura-2/AM. Loading endothelial cells through the patch pipette with 40 mmol/l [Na⁺] did not induce significant changes of [Ca²⁺]_i. Superfusing the cells with K⁺-free solutions also did not significantly affect [Ca²⁺]_i. Reapplication of K⁺ after superfusion of the cells with K⁺-free solution induced an outward current at a holding potential of 0 mV. This current was nearly completely blocked by 100 mol/l dihydroouabain (DHO) and was therefore identified as a Na⁺/K⁺ pump current. During block and reactivation of the Na⁺/K⁺ pump no changes in [Ca²⁺]_i could be observed. Pump currents were blocked concentration dependently by DHO. The concentration for half-maximal inhibition was 21 mol/l. This value is larger than that reported for other tissues and the block was practically irreversible. Insulin (10–1000 U/l) did not affect the pump currents. An increase of the intracellular Na⁺ concentration ([Na⁺]_i) enhanced the amplitude of the pump current. Half-maximal activation of the pump current by [Na⁺]_i occurred at about 60 mmol/l. The concentration for half-maximal activation by extracellular K⁺ was 2.4±1.2 mmol/l, and 0.4±0.1 and 8.7±0.7 mmol/l for Tl⁺ and NH₄ ⁺ respectively. The voltage dependence of the DHO-sensitive current was obtained by applying linear voltage ramps. Its reversal potential was more negative than –150 mV. Pump currents measured with the conventional whole-cell technique were about four times smaller than pump currents recorded with the nystatin-perforated-patch method. If however 100 mol/l guanosine 5-O-(3-thiotriphosphate) (GTPS) were added to the pipette solution, the currents measured in the ruptured-whole-cell-mode were not significantly different from the currents measured with the perforated-patch technique. We suppose that the use of the perforated-patch technique prevents wash out of a guanine nucleotide-binding protein (G-protein)-connected intracellular regulator that is necessary for pump activation.     

Characteristics of single Na+ channels of adult human skeletal muscle

The patch-clamp technique was used to study Na⁺ channels of human skeletal muscle. Preparations were from biopsies of quadriceps muscle from adults who were not suffering from neuromuscular diseases. Activity of Na⁺ channels was recorded from inside-out patches when the membrane potential was stepped from a holding potential of ±110mV to potential above a threshold of about ±65 mV. Single channel activity increased within minutes after hyperpolarizing the patch due to recovery from ultraslow inactivation. Up to ten Na⁺ channels were active in individual patches. Macroscopic currents were reconstructed by averaging single channel currents. The time-to-peak current declined from 1.6 ms at ±60 mV to 0.5 ms at +10 mV. The currents decayed mono-exponentially with time constants between 12.1 ms at ±60 mV and 0.4 ms at +10 mV (21°C). The conductance of single Na⁺ channels was 1.65 pS and the mean open time was voltage-dependent. At ±50 mV, the mean open time was 0.4 ms, while positive to ±10 mV it increased to values above 1 ms. In the threshold potential range, the number of openings per depolarizing pulse was larger than the number of channels under the patch-clamp pipette, indicating reopening of Na⁺ channels at this potential. Openings could be observed only rarely 10 ms after onset of depolarization and the macroscopic current produced by late openings was less than 0.1% of the peak current. Human skeletal muscle is thus suitable for investigation with the patch-clamp technique and the determination of properties of Na⁺ channels with this technique could be the basis for an assessment of possible defects of these channels in diseased muscle.     

Estragole blocks neuronal excitability by direct inhibition of Na+ channels

Estragole is a volatile terpenoid, which occurs naturally as a constituent of the essential oils of many plants. It has several pharmacological and biological activities. The objective of the present study was to investigate the mechanism of action of estragole on neuronal excitability. Intact and dissociated dorsal root ganglion neurons of rats were used to record action potential and Na⁺ currents with intracellular and patch-clamp techniques, respectively. Estragole blocked the generation of action potentials in cells with or without inflexions on their descendant (repolarization) phase (N_inf and N₀ neurons, respectively) in a concentration-dependent manner. The resting potentials and input resistances of N_inf and N₀ cells were not altered by estragole (2, 4, and 6 mM). Estragole also inhibited total Na⁺ current and tetrodotoxin-resistant Na⁺ current in a concentration-dependent manner (IC₅₀ of 3.2 and 3.6 mM, respectively). Kinetic analysis of Na⁺ current in the presence of 4 mM estragole showed a statistically significant reduction of fast and slow inactivation time constants, indicating an acceleration of the inactivation process. These data demonstrate that estragole blocks neuronal excitability by direct inhibition of Na⁺ channel conductance activation. This action of estragole is likely to be relevant to the understanding of the mechanisms of several pharmacological effects of this substance.     

Tetrodotoxin differentially blocks peak and steady-state sodium channel currents in early embryonic chick ventricular myocytes

Single ventricular myocytes were dissociated from 3-day-old embryonic chick hearts and maintained in culture for 9–21 h. The whole-cell patch clamp method was used to record tetrodotoxin (TTX)-sensitive fast Na⁺ currents. The peak Na⁺ current recorded at –20 mV ranged from 10 to 70 A/cm². At more negative potentials, a component of the current decayed very slowly, resulting in a significant steady-state or late Na⁺ current. The origin of the late Na⁺ current was revealed through the examination of single Na⁺ channel currents recorded in outside-out membrane patches. The single Na⁺ channel conductance was 20 pS. A high percentage of the trials ( 16%) displayed multiple reopenings of a single Na⁺ channel, resulting in bursts of current lasting for 150 ms. The frequency distributions of the Na⁺ channel open-times were bi-exponential. The burst-like mode of Na⁺ channel activity (which underlies the slowly- or non-inactivating currents recorded macroscopically), was blocked to a greater degree by TTX, compared to the peak current. The results suggest that differential blockade may occur as a result of the slow binding and increased affinity of TTX to the open Na channel.     

Identification of a non-selective cation channel current in myometrial cells isolated from pregnant rats

Non-selective cation channel (NSCC) currents were identified in myometrial smooth muscle cells isolated from pregnant rats (day 18–20) using the whole-cell patch clamp method. NSCC currents had a linear current/voltage relationship and were time independent. Reduction of extracellular Na⁺ substantially decreased the amplitude of NSCC currents, indicating that the NSCC is permeable to Na⁺. NSCC currents were blocked by La³⁺ and Gd³⁺ with K _d values of 2.2 and 1.0 µM, respectively. The relative permeability of various monovalent cations for NSCC was estimated by measurement of the reversal potential. The relative permeability for K⁺:Cs⁺:Na⁺:Li⁺ was 1.3:1:0.9:0.8. NSCC also had a small, but detectable, permeability for Ca²⁺. Extracellular Mg²⁺ inhibited myometrial NSCC currents concentration dependently with a K _d of 0.28 mM. The observed Mg²⁺ block may reasonably explain the inhibitory effect of magnesium on uterine contractions in the treatment of pre-term labor. Our results suggest that the NSCC plays a role in the regulation of myometrial contractility.     

Potassium channels in the basolateral membrane of the rectal gland of the dogfish (Squalus acanthias)

Previous studies in isolated, in vitro perfused rectal gland tubules (RGT) have revealed that the basolateral membrane possesses a K⁺ conductive pathway. In the present study, we have utilized the patch clamp technique in RGT segments to characterize this pathway. The basolateral membrane was approached with patch pipettes at the open end of in vitro perfused segments [5]. Recordings were obtained in cell-attached as well as in excised inside-out patches. In cell-attached patches with the pipette filled with a KCl solution (274 mmol/l) and the bath containing NaCl shark Ringer (275 mmol/l), inward K⁺ currents (from pipette into cell) with a mean slope conductance of 123±26 pS (n=3) were observed. We were unable to generate outward K⁺ currents at high depolarizing (cell more positive) clamp voltages. This indicates inward rectification of this channel. To examine the rectification properties further, excised (inside out) patches were exposed to K⁺ concentration gradients, directed out of, as well as into the pipette. With NaCl in the pipette and KCl in the bath, K⁺ outward currents were observed. The current-voltage (IV) relation revealed Goldman-type rectification, with a mean single channel conductance of 185±28 pS (n=7) at high positive voltages (linear range of the IV curve). The single-channel permeability coefficient for K⁺ was 0.26±0.04 ·10^–12 cm³/s (n=7). In the reversed experiment (pipette KCl, bath NaCl), inward currents of similar kinetics and amplitude were obtained. The single channel conductance was 146±21 pS (n=7) at high negative voltages (linear range of the IV curve). The single channel permeability coefficient for K⁺ was 0.21±0.03·10^–12 cm³/s (n=7). We were not able to reverse the currents in any of these experiments, indicating that this channel is highly selective for K⁺ over Na⁺. In all three series of experiments, the kinetic appearance of the channels was similar. Bursts of activity were followed by interburst pauses. The open state was described by a single time constant of 3.0±0.2 ms, whereas the closed state was described by two time constants of 0.7±0.2 ms and 2.8±0.5 ms (n=8). It can be concluded that these channels permit K⁺ inward and outward currents. They are probably the equivalent of the basolateral K⁺ conductance as observed in a previous study [12]. Under physiological conditions a single channel conductance of some 20 pS is predicted from the present data. In cell-attached patches, with a high K⁺ concentration in the pipette, the channel behaves as an inward rectifier.Supported by Deutsche Forschungsgemeinschaft Gr 4808 and by NSF and NIH grants to the MDIBL. Parts of this study have been published in the Mount Desert Island Biol. Bulletin 1984, 1985.