阿司匹林对代谢综合征患者氧化应激指标和P选择素及C反应蛋白的影响

目的观察阿司匹林对代谢综合征(MS)患者氧化应激指标、可溶性P-选择素(sPE)及C反应蛋白(CRP)的影响。方法收集年龄、性别匹配的MS患者(MS组)27例,健康组29例。MS组口服阿司匹林100mg,1/d。比较MS组和健康组超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH)、氧化低密度脂蛋白(ox-LDL)、sPE以及CRP水平,以及MS组应用阿司匹林1个月后上述指标的变化。结果 MS组血清SOD、GSH水平较健康组显著降低,血清MDA、ox-LDL、CRP和sPE显著升高(P<0.05、P<0.01);应用阿司匹林1个月后,血清GSH水平显著升高,MDA、ox-LDL、CRP和sPE水平显著降低(P<0.05)。结论该剂量阿司匹林可能改善MS患者氧化应激、炎性反应和sPE的改变。     

血清脂联素水平与代谢综合征组分的相关性研究

目的 探讨血清脂联素水平与代谢综合征(metabolic syndrome,MS)各组分的相关性.方法 采用整群随机抽样的方法,抽取上海市的两个城区,经调查和体检,筛选出MS患者235例.通过对MS患者的脂联素水平与MS各组分(腰围、血压、血糖、血脂)相关性分析及主成分回归分析,初步探讨血清脂联素水平与MS各组分的关系.结果 男性脂联素水平为(2.82±1.73)μg/ml低于女性的(5.12±3.51)μg/ml,差异有统计学意义(Z=-5.25,P=0.00).脂联素水平[(4.38±3.23)μg/ml]与腹部肥胖相关,其与腰围[(93.37±7.76)cm]的spearrnan秩相关系数为rs=-0.18(tt=-2.79,P=0.01);与腰臀比(0.91±0.05)的spearman秩相关系数为rs=-0.28(tr=-4.44,P=0.00),而与人体质量指数(body mass index,BMI)[(26.85±3.10)ks/m2]无显著相关性(rs=-0.10,tr=-1.45,P=0.15).脂联素与胰岛素抵抗相关指标关系密切,包括空腹血糖[(5.48±1.72)mmol/L](rs=-0.13,tr=-2.00,P=0.05)、空腹胰岛素[(11.52±5.34)mU/L](rs=-0.15,tr=-2.31,P=0.02)、稳态模式评估法的胰岛素抵抗指数(HOMA-IR)(2.90±2.07)(rs=-0.17,tr=-2.63,P=0.01)及甘油三酯水平[(2.11±1.24)mmol/L](rs=-0.24,tr=-3.77,P=0.00).脂联素与总胆固醇水平[(5.01±1.19)mmol/L](rs=-0.05,tr=-0.78,P=0.44)、高密度脂蛋白胆固醇(HDL-C)[(1.21±0.32)mmol/L](rs=0.06,tr=0.93,P=0.36)、收缩压[(131.40±14.93)nun Hg,1 nun Hg=0.133 kPa](rs=-0.02,tr=-0.34,P=0.73)及舒张压[(86.77±9.14)mm Hg](rs=-0.02,tr=-0.27,P=0.78)无显著相关性.结论 MS患者血清脂联素水平与腹部肥胖及胰岛素抵抗相关指标呈负相关,而与血压未见直接联系.     

HIV感染者抗病毒治疗后HIV-1辅助受体的利用情况

目的 调查HIV-1感染人群抗病毒治疗后HIV-1辅助受体的利用情况.方法 从安徽、河南两地选择109例接受抗病毒治疗的HIV-1感染者和45例未接受抗病毒治疗的HIV-1感染者作为研究对象,通过周围血单个核淋巴细胞(PBMCs)共培养方法,分离培养HIV-1临床毒株,利用酶联免疫吸附法检测培养上清HIV-1的P24含量,通过表达趋化因子CCR5和CXCR4的Ghost细胞系检测病毒辅助受体利用,对治疗人群中HIV-1辅助受体利用因素进行探讨,并与治疗因素进行分析讨论.结果 从治疗人群中分离了45株病毒,其中22株病毒(48.89%)利用CCR5辅助受体,是R5嗜性毒株;21株病毒(46.67%)利用CXC4/CCR5辅助受体,为双嗜性(X4/R5)毒株;有2株(4.44%)仅利用CXCR4辅助受体,为X4嗜毒株.从未治疗人群中分离了109株病毒,其中96株(88.07%)利用CCR5辅助受体(R5嗜性毒株);13株(11.93%)为X4/R5双嗜性毒株.HIV-1 CXC4/CCR5辅助受体利用率在两组中的差异有统计学意义(x2=27.30,P＜0.05).治疗方案一(齐多夫定、去羟肌苷、奈韦拉平三联用药)治疗后HIV-1 CXC4/CCR5的利用率为59.09%(13/22),治疗方案二(司他夫定、去羟肌苷、奈韦拉平三联用药)治疗后HIV-1 CXC4/CCR5的利用率为43.48%(10/23),二者对HIV-1辅助受体利用的影响差异没有统计学意义(x2=1.10,P=0.30).结论 在HIV-1感染者中,接受抗病毒治疗人群中HIV-1 CXCR4/CCR5利用率高于未治疗人群.     

Takotsubo综合征患者系统氧化应激与心脏功能相关性研究

目的Takotsubo综合征(TS)是一种病理机制非常复杂的心脏急症，其症状与急性冠脉综合征(ACS)相似，容易被误诊。TS人群预后不良，短期病死率与急性心肌梗死(AMI)患者相似，长期主要心血管事件发生率与ACS患者相当。本研究通过临床研究探索系统氧化应激与TS的相关性。 方法收集临床以TS为第一诊断的住院患者共计12例，同时纳入条件匹配的AMI者和健康体检者为对照，记录人口学资料，使用床边超声评估三组人群心脏功能，通过检测血浆MDA水平和炎症因子浓度比较系统炎症水平。 结果与AMI人群和健康对照人群相比较，TS患者心脏功能明显下降，血浆MDA浓度增高，炎症细胞水平上调和炎症因子分泌增多。 结论TS患者系统氧化应激水平增高和炎症反应激活。     

Association between serum levels of vascular endothelial growth factor,macrophage inhibitory cytokine and markers of oxidative stress,with the metabolic syndrome and its components in obese individuals

Aim

Metabolic syndrome (MetS) is a clinical disorder with widespread prevalence. In the current study, we aimed to investigate the serum values of macrophage inhibitory cytokine (MIC)-1, vascular endothelial growth factor (VEGF) and markers of oxidative stress among patients with metabolic syndrome.

代谢紊乱患者血清脂联素水平与冠状动脉病变程度的关系

目的探讨代谢紊乱患者血清脂联素水平及其与冠状动脉病变程度的关系。方法100例患者分为代谢综合征组和对照组，分别行冠状动脉造影检查及血清脂联素水平的检测，对比各组血清脂联素水平和冠状动脉病变程度的差异。结果（1）代谢综合征组空腹血糖（FPG）、三酰甘油（TG）、稳态模型评估法计算的胰岛素抵抗指数（HOMA-IRI）、体重指数、腰围、收缩压、舒张压和Gensini评分均高于对照组，血清脂联素和高密度脂蛋白-胆固醇（HDL-C）水平低于对照组（P〈0．01）。（2）随着代谢紊乱数目的增加，血清脂联素水平逐渐下降，Gensini评分逐渐升高（P〈0．05或〈0．01）。1项及以下代谢紊乱者与2项及以上代谢紊乱者之间的冠心病患者比例比较，差异有统计学意义（P〈0．01）。（3）脂联素与HDL-C呈正相关，与Gensini评分、TG及代谢紊乱数目呈负相关。结论代谢紊乱患者随着代谢紊乱数目的增加，血清脂联素水平降低，冠状动脉粥样硬化程度加重。     

Immunization with an HIV-1 immunogen induces CD4+ and CD8+ HIV-1-specific polyfunctional responses in patients with chronic HIV-1 infection receiving antiretroviral therapy

Development of polyfunctional T lymphocyte responses is critical in the immunological response against HIV-1. Fifty-four HIV-1 infected patients receiving antiretroviral treatment (ART) and immunization with an HIV-1 immunogen or placebo, periodically every 3 months throughout a period of 36 months, were evaluated for the purposes of analysing the development of HIV-1-specific CD4⁺ and CD8⁺ responses. A significant increase of proliferating and IFN-γ producing CD8⁺ HIV-1-specific T cells, of HIV-1-specific precursor frequencies for CD8⁺ and for CD4⁺ T cells and of Gag/pol-specific memory CTL precursors (CTLp) was observed in the immunogen group in comparison to placebo. IL-2 intracellular expression and IFN-γ and TNF- co-expression in HIV-1-specific CD8⁺ T cells were also substantially increased in the immunized group. A negative correlation between viral load and CD3⁺CD4⁺CFSE^low HIV-1-specific lymphoproliferative response and frequency of Gag/pol-specific CTLp was solely observed in the HIV-1 immunogen group. Long-term immunization in patients receiving ART helps to develop HIV-1-specific polyfunctional T cell responses.     

男性警察职业紧张与代谢综合征的关系

目的 探讨导致男性警察代谢综合征(metabolic syndrome,MS)患病的职业紧张因素.方法 采用随机整群抽样的方法,从某市公安局现管辖的分局中抽取4个分局,以该4个分局的全体男性警察作为调查对象,共纳入1490名.采用统一设计的健康调查问卷和职业紧张量表进行流行病学调查,同时进行体格检查和生化指标检测,应用χ2检验和logistic回归模型进行分析.结果 1490名研究对象中,实际完成问卷1483名,合格问卷1480份,其中MS患者有237例,患病率为16.0%(237/1480).高度、中度及缺乏职业紧张者MS组分别有8、39和23例,非MS组有14、114和131例,高度与中度缺乏职业紧张者MS患病风险分别为缺乏职业紧张者的4.82倍(95%CI:1.50～15.41)和3.33倍(95%CI:1.62～6.79).任务过重、任务不适和责任感MS组得分分别为(38.76±6.83)、(25.74±7.22)、(25.76±6.27)分;非MS组得分分别为(37.55±6.85)、(24.50±6.58)、(25.05±5.95)分.多因素logistic回归分析显示,任务过重、任务不适和责任感可能为MS患病的职业紧张危险因素,OR(95%CI)值分别为1.06(1.02～1.10)、1.04(1.02～1.07)、1.03(1.01～1.06).结论 任务过重、任务不适和责任感为导致男性警察MS患病的职业紧张危险因素.

Abstract：

Objective To explore the occupational stress factors associated with the prevalence of metabolic syndrome(MS) among male policemen.Methods Using cluster sampling method,we selected four Public Security Bureau within the jurisdiction of the station now in somesity.All the male police were included as research objects,and finally 1490 persons were selected,health and occupational stress inventory-revised (OSI-R) questionnaire were used for epidemiological surveys,and anthropometric examination and chemical indicators were also measured at the same time.The analysis methods were chi-square test and unconditional logistical regression.Results Among the 1490 of research objects,1483 completed the questionnaire,and 1480 of the eligible questionnaires were available.237 cases were MS,and the prevalence rate was 16.0%(237/1480).The number of cases who were high,moderate and lack of occupational stress in MS group were 8,39 and 23,that in non-MS were 14,114 and 131,respectively.The odds of occupational stress with the highest and medium among policemen than who were lack were 4.82 (95%CI:1.50-15.41) and 3.33(95%CI:1.62-6.79); the average score of role ambiguity,role insufficiency and responsibility in the group of MS were (38.76±6.83),(25.74±7.22),(25.76±6.27);and that in non-MS were (37.55±6.85),(24.50±6.58),(25.05±5.95).The logistical regression analysis showed that : the likely three occupational risk stress factors which influencing the prevalence of MS were role ambiguity,role insufficiency and responsibility,and the OR(95%CI) were 1.06 (1.02-1.10),1.04 (1.02-1.07) and 1.03 (1.01-1.06),respectively.Conclusion Role ambiguity,role insufficiency and responsibility were the occupational risk stress factors associated with the prevalence of MS among male policemen.     

多囊卵巢综合征患者血清脂联素检测的意义

目的:探讨多囊卵巢综合征(polycystic ovary syndrom e,PCOS)患者血清脂联素与胰岛素抵抗的关系。方法:将42例PCOS患者分为非肥胖组(26例)和肥胖组(16例),28例正常妇女分为非肥胖组(19例)和肥胖组(9例)。测定黄体生成素(LH)、卵泡刺激素(FSH)、睾酮(T)、胰岛素(INS)、空腹血糖(SG)、脂联素浓度。结果:PCOS组LH、LH/FSH、T均显著高于对照组;与非肥胖对照组相比,其他3组脂联素浓度显著降低,胰岛素抵抗指数(HOMA-IR)值显著升高;血清中脂联素水平与体重指数(BM I)、HOMA-IR、T值均呈显著负相关。结论:PCOS患者可能存在脂联素缺乏,且程度与胰岛素抵抗有关。脂联素具有广阔的药物开发前景。     

罗格列酮对非糖尿病代谢综合征患者血清脂联素水平的影响

目的研究非糖尿病代谢综合征患者服用罗格列酮之后血清脂联素水平的变化,分析罗格列酮干预对血清脂联素的影响。方法选择非糖尿病代谢综合征患者92例,应用酶联免疫吸附法测定所有受试者血清脂联素水平,将入选患者随机分为两组,A组40例,给予低盐低脂饮食、常规降压及降血脂药物治疗;B组52例,在上述治疗的基础上,加用过氧化体增殖物激活受体-γ（PPAR—γ）激动剂罗格列酮（4mg／d）口服,8周后复查脂联素水平。结果（1）A组治疗前后脂联素水平比较无统计学差异（1．49±0．59mg／1,1．21±0．66mg／1,P〉0．05）;（2）B组治疗前后比较,脂联素水平升高,有统计学差异（0．89±0．73mg／1,2．52±1．37mg／1,P〈0．01）。结论非糖尿病代谢综合征患者经口服PPAR—γ激动剂罗格列酮后脂联素水平明显升高,提示罗格列酮可提高非糖尿病代谢综合征患者脂联素水平。     

Effects of pistachio nuts on body composition,metabolic, inflammatory and oxidative stress parameters in Asian Indians with metabolic syndrome: A 24-wk,randomized control trial

ObjectiveThe aim of this study was to evaluate the effects of pistachio nuts as an adjunct to diet and exercise on body composition, metabolic, inflammatory, and oxidative stress parameters in Asian Indians with metabolic syndrome.MethodsIn this 24-wk randomized control trial, 60 individuals with the metabolic syndrome were randomized to either pistachio (intervention group) or control group (diet as per weight and physical activity profile, modulated according to dietary guidelines for Asian Indians) after 3 wk of a diet and exercise run in. In the first group, unsalted pistachios (20% energy) were given daily. A standard diet and exercise protocol was followed for both groups. Body weight, waist circumference (WC), magnetic resonance imaging estimation of intraabdominal adipose tissue and subcutaneous abdominal adipose tissue, fasting blood glucose (FBG), fasting serum insulin, glycosylated hemoglobin, lipid profile, high-sensitivity C-reactive protein (hs-CRP), adiponectin, free fatty acids (FFAs), tumor necrosis factor (TNF)-α, leptin, and thiobarbituric acid reactive substances (TBARS) were assessed before and after the intervention.ResultsStatistically significant improvement in mean values for various parameters in the intervention group compared with control group were as follows: WC (P < 0.02), FBG (P < 0.04), total cholesterol (P < 0.02), low-density lipoprotein cholesterol (P < 0.006), hs-CRP (P < 0.05), TNF-α (P < 0.03), FFAs (P < 0.001), TBARS (P < 0.01), and adiponectin levels (P < 0.001).ConclusionA single food intervention with pistachios leads to beneficial effects on the cardiometabolic profile of Asian Indians with metabolic syndrome.     

阜新市HIV-1感染者和AIDS患者原发性整合酶基因耐药突变分析

目的 了解阜新市HIV感染者和AIDS患者整合酶基因的变异情况,分析阜新市原发性整合酶基因突变及相关耐药情况,为治疗提供参考依据.方法 收集2018年-2019年阜新市102例HIV-1感染者和AIDS患者外周静脉血,分离血浆,-80℃冻存备用.采用反转录巢式聚合酶链式反应扩增病毒pol基因区并进行序列测定,分析IN区...     

天津市不同感染途径HIV-1感染者流行毒株亚型分析

目的 了解天津市HIV-1毒株的流行情况,比较不同感染途径HIV-1感染者中流行毒株的亚型分布.方法 采集100例感染途径已知的HIV-1感染者抗凝全血标本,提取DNA,用巢式聚合酶链式反应扩增病毒gag基因,并进行序列测定和亚型分析.结果 100份样品中,有87份样品被成功扩增出HIV-1的gag基因片段,通过系统进化分析,确定天津市HIV-1流行毒株分属4个亚型和重组型,其中CRF01 _AE比例最大,达59.77％(52/87),主要分布在通过同性和异性性传播的HIV-1感染者中,而在通过静注吸毒感染的HIV-1感染者中以CRF07_BC亚型为主.结论 天津市HIV-1流行株至少有4种基因亚型,且不同感染途径HIV-1感染者流行毒株的亚型分布略有不同,应加强对HIV-1毒株亚型变异的监测,及时调整防治策略.