Effect of the magnetized water supplementation on blood glucose,lymphocyte DNA damage,antioxidant status,and lipid profiles in STZ-induced rats

This study investigated the effects of magnetized water supplementation on blood glucose, DNA damage, antioxidant status, and lipid profiles in streptozotocin (STZ)-induced diabetic rats. There were three groups of 4-week-old male Sprague-Dawley rats used in the study: control group (normal control group without diabetes); diabetes group (STZ-induced diabetes control); and magnetized water group (magnetized water supplemented after the induction of diabetes using STZ). Before initiating the study, diabetes was confirmed by measuring fasting blood glucose (FBS > 200 dl), and the magnetized water group received magnetized water for 8 weeks instead of general water. After 8 weeks, rats were sacrificed to measure the fasting blood glucose, insulin concentration, glycated hemoglobin level, degree of DNA damage, antioxidant status, and lipid profiles. From the fourth week of magnetized water supplementation, blood glucose was decreased in the magnetized water group compared to the diabetes group, and such effect continued to the 8th week. The glycated hemoglobin content in the blood was increased in the diabetes group compared to the control group, but decreased significantly in the magnetized water group. However, decreased plasma insulin level due to induced diabetes was not increased by magnetized water supplementation. Increased blood and liver DNA damages in diabetes rats did significantly decrease after the administration of magnetized water. In addition, antioxidant enzyme activities and plasma lipid profiles were not different among the three groups. In conclusion, the supplementation of magnetized water not only decreased the blood glucose and glycated hemoglobin levels but also reduced blood and liver DNA damages in STZ-induced diabetic rats. From the above results, it is suggested that the long-term intake of the magnetized water over 8 weeks may be beneficial in both prevention and treatment of complications in diabetic patients.     

三种食物多酚对化学诱导DM大鼠糖脂代谢的影响

目的探讨膳食多酚[绿原酸、表没食子儿茶素没食子酸酯(EGCG)、槲皮素]对链脲佐菌素(STZ)诱导的糖尿病大鼠血糖、血脂、肝脏中葡萄糖-6-磷酸酶(G-6-pase)和骨胳肌组织中葡萄糖转运体4(GLUT4)表达的影响。方法单次腹腔注射链脲佐菌素(STZ,35 mg/kg)结合高脂饮食建立糖尿病大鼠模型,将成模大鼠分成5组[糖尿病模型组(DM)、糖尿病模型+二甲双胍组(S)、糖尿病模型+绿原酸组(CA)、糖尿病模型+EGCG组(E)、糖尿病模型+槲皮素组(Q)],另设正常对照组(NC),分别灌喂二甲双胍、绿原酸、EGCG和槲皮素4w后,测定其糖耐量、空腹胰岛素、甘油三酯、胆固醇、G-6-pase和GLUT4 mRNA的表达。结果绿原酸、EGCG、槲皮素均表现出改善STZ诱导的糖尿病大鼠血糖、甘油三酯(TG)和胆固醇(TC)的含量,并能改善糖尿病模型大鼠的胰岛素敏感性,抑制肝脏G-6-pase mRNA的表达,且提高了骨胳肌GLUT4 mRNA的表达,其中以绿原酸效果最佳。仅仅其糖耐量改善弱于槲皮素作用,但均弱于阳性对照组二甲双胍的作用。结论绿原酸、EGCG、槲皮素均能有效改善STZ诱导的SD糖尿病大鼠的糖代射、脂代射、胰岛素敏感生及肝脏G-6-pase mRNA和骨胳肌GLUT4 mRNA的表达,绿原酸的效果最佳。[营养学报,2012,34(6):572-575,581]     

明日叶查尔酮对2型糖尿病大鼠肝细胞胰岛素受体与胰岛素受体底物-2 mRNA表达的影响

目的研究明日叶查尔酮(Ashitabe chalcone,AC)对糖尿病大鼠肝细胞胰岛素受体(insulin receptor,InsR)和胰岛素受体底物-2(insulin receptor substrate-2,IRS-2)mRNA表达的影响。方法将用高脂饲料喂养加链尿佐菌素腹腔注射诱发的2型糖尿病大鼠随机分为糖尿病对照组和高、低剂量AC组,每组10只,均喂饲高脂饲料,分别每日经口灌胃给予明日叶查尔酮的剂量为0、30、10 mg/kg。另设一个正常对照组为正常大鼠喂饲普通饲料,实验周期4 w。用放射免疫分析法检测血清胰岛素水平、逆转录聚合酶链式反应方法检测肝细胞InsR和IRS-2 mRNA表达水平、免疫组化法测肝细胞InsR蛋白表达水平、葡萄糖氧化酶法测血糖含量。结果与正常对照组比较,糖尿病对照组空腹血糖和血清胰岛素升高,而InsR和IRS-2 mRNA表达水平降低。与糖尿病组比较,高剂量AC组空腹血糖和血清胰岛素降低,而InsR和IRS-2mRNA表达水平升高,各项差异均有显著性意义(P<0.05)。结论明日叶查尔酮可上调2型糖尿病大鼠肝细胞InsR和IRS-2mRNA表达水平,改善胰岛素抵抗状况。     

Anti-diabetic and hypolipidaemic properties of ginger (Zingiber officinale) in streptozotocin-induced diabetic rats

In the present study, the hypoglycaemic potentials of ginger (Zingiber officinale) were studied in rats. An aqueous extract of raw ginger was administered daily (500 mg/kg, intraperitoneally) for a period of 7 weeks to streptozotocin (STZ)-induced diabetic rats. Fasting blood serum was analysed for blood glucose, cholesterol and triacylglycerol levels. The STZ-injected rats exhibited hyperglycaemia accompanied with weight loss, indicating their diabetic condition. At a dose of 500 mg/kg, raw ginger was significantly effective in lowering serum glucose, cholesterol and triacylglycerol levels in the ginger-treated diabetic rats compared with the control diabetic rats. The ginger treatment also resulted in a significant reduction in urine protein levels. In addition, the ginger-treated diabetic rats sustained their initial weights during the treatment period. Moreover, ginger decreased both water intake and urine output in the STZ-induced diabetic rats. The present results indicate that raw ginger possesses hypoglycaemic, hypocholesterolaemic and hypolipidaemic potential. Additionally, raw ginger is effective in reversing the diabetic proteinuria observed in the diabetic rats. Thus, ginger may be of great value in managing the effects of diabetic complications in human subjects.     

Effects of chard (Beta vulgaris L. var. cicla) extract on oxidative injury in the aorta and heart of streptozotocin-diabetic rats

In diabetes mellitus, increased free radical formation raises the incidence of atherosclerosis and cardiovascular diseases. Regardless of the type of diabetes, the objective of the therapy is to achieve normoglycemia and to prevent or delay the complications. Chard (Beta vulgaris L. var. cicla) is used as a hypoglycemic agent by diabetic patients in Turkey. The aim of this study was to investigate the effect of feeding chard on diabetes-induced free radical-mediated injury in rat aorta and heart tissues. Female Swiss albino rats were randomly divided into four groups: control, diabetic, chard, and diabetic + chard. Rats were subjected to intraperitoneal streptozotocin (STZ, 65 mg/kg) to induce diabetes. Chard extract (2 g/kg) was given for 28 days beginning on the 14th day of the study. Aorta and heart tissue lipid peroxidation and glutathione levels as well as blood glucose levels were determined. The results of the present study indicate that lipid peroxidation was increased and glutathione levels were decreased in both aorta and heart tissue of the diabetic rats. However, treatment with chard extract reversed the effects of diabetes on blood glucose and tissue lipid peroxidation and glutathione levels.     

运动对糖尿病大鼠肥胖蛋白的作用

为了观察适量运动对糖尿病大鼠肥胖蛋白、胰岛素及血糖的影响 ,以探讨适量运动改善糖尿病大鼠糖代谢紊乱的可能机制。将 40只SD大鼠分为 4组 :糖尿病非运动组、糖尿病运动组、正常非运动组和正常运动组。运动组进行 12周的中等强度的跑步训练。结果显示 :(1)糖尿病大鼠血糖浓度增高 ,血胰岛素及血C肽浓度降低 ;血浆肥胖蛋白水平下降 ,脑组织和脂肪组织中肥胖蛋白水平下降 ;(2 )糖尿病运动组大鼠经 12周跑步训练后 ,血糖浓度下降 ,血胰岛素和C肽浓度升高 ;血浆及脑组织和脂肪组织肥胖蛋白水平相应上升。提示适量运动通过对肥胖蛋白和胰岛素的作用改善糖尿病的能量代谢失衡状态 ,这可能是运动改善糖尿病糖代谢紊乱的机制之一。     

明日叶查尔酮对糖尿病大鼠肝细胞PI3K和AktmRNA表达的影响

目的研究明日叶查尔酮(ashitabe chalcone,AC)对糖尿病大鼠肝细胞磷脂酰肌醇3激酶(phosphatidy I inositol 3-kinase,PI3K)和蛋白激酶(serine-threonine kinases,Akt)mRNA表达的影响。方法高脂饲料喂养加链尿佐菌素腹腔注射诱发的2型糖尿病大鼠随机分为糖尿病对照组和高、低剂量AC组,每组10只,均喂饲高脂饲料,分别每日经口灌胃给予明日叶查尔酮0、30和10mg/kg BW。另设一个正常对照组为正常大鼠喂饲普通饲料,实验周期4周。用放射免疫分析法检测血清胰岛素水平,葡萄糖氧化酶法测血糖含量,逆转录聚合酶链式反应方法检测肝细胞PI3K和Akt mRNA表达水平,蛋白印迹法测肝细胞Akt磷酸化水平。结果高剂量AC组空腹血糖和血清胰岛素水平显著低于糖尿病对照组,而PI3K mRNA、Akt mRNA和磷酸化Akt蛋白表达水平均显著高于糖尿病对照组,差异均具有统计学意义(P<0.05)。结论明日叶查尔酮可上调糖尿病大鼠PI3K和Akt mRNA的表达水平,改善胰岛素抵抗。     

Effect of different vitamin E levels on lipid peroxidation in streptozotocin-diabetic rats.

Administration of streptozotocin is used to induce diabetes in experimental models, causing a selective destruction of pancreatic beta islet cells associated with generation of free radicals. Supplementation with antioxidant vitamins such as vitamin E is a protective factor against free radicals. The objective of this study was to determine the effect of administration of a diet supplemented with, or deficient in vitamin E to streptozotocin diabetic rats, controlled or not with insulin, on plasma glucose, hepatic vitamin E and hepatic thiobarbituric acid reactive substance (TBARS) levels before streptozotocin and 24 hours and one and two weeks after drug administration. Deficiency of vitamin E alone increased TBARS levels, and streptozotocin elevated TBARS two times in deficient groups, regardless of insulin control. In rats supplemented with vitamin E, a reduction of plasma glucose and liver vitamin E was observed two weeks after streptozotocin administration (p < 0.05). In conclusion, vitamin E supplementation probably protected against lipoperoxidation and contributed to the absence of elevation of plasma glucose levels, and vitamin E deficiency produced an increase in hepatic TBARS levels in streptozotocin diabetic rats.     

Modulatory effect of Scoparia dulcis in oxidative stress-induced lipid peroxidation in streptozotocin diabetic rats

In light of evidence that diabetes mellitus is associated with oxidative stress and altered antioxidant status, we investigated the effect of Scoparia dulcis plant extracts (SPEt) (aqueous, ethanolic, and chloroform) in streptozotocin diabetic rats. Significant increases in the activities of insulin, superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, reduced glutathione, vitamin C, and vitamin E were observed in liver, kidney, and brain on treatment with SPEt. In addition, the treated groups also showed significant decreases in blood glucose, thiobarbituric acid-reactive substances, and hydroperoxide formation in tissues, suggesting its role in protection against lipid peroxidation-induced membrane damage. Thus, the results of the present study indicate that extracts of S. dulcis, especially the aqueous extract, showed a modulatory effect by attenuating the above lipid peroxidation in streptozotocin diabetes.     

Maitake (Grifola frondosa) improve glucose tolerance of experimental diabetic rats

We have previously reported that rats with diabetes induced by injecting streptozotocin into neonates showed remarkably lower blood glucose, urine volume, and glucosuria after administration of Maitake (Grifola frondosa). In the present study, we investigated the effects of Maitake on insulin concentration, organ weight, serum composition, and islets of Langerhans in streptozotocin-induced diabetic rats using the same method. The diabetic rats were produced by injecting 80 mg/kg B.W. streptozotocin into 2-d-old neonates. From the age of 9 wk, the rats were given experimental diets for 100 d. The diabetes and control groups were given either diets containing 20% Maitake (DM and CM groups) or control diets (D and C groups). During administration of the experimental diets, we measured body weight, food intake, amount of feces, and serum insulin concentration at glucose loading. The glucose tolerance test was performed at the 10th week after the start of the experimental diets. The D group had an initial fasting blood glucose of 225+/-49 mg/dL, and a maximum blood glucose of 419+/-55 mg/dL at 60 min. In the DM group, however, the initial fasting blood glucose was 170+/-23 mg/dL, and the maximum blood glucose was 250+/-41 mg/dL at 15 min. Both values were markedly lower than those in the D group (p<0.05). The insulin concentration at 15 min. after glucose loading in the DM group was 41+/-16 microU/mL, which was significantly higher than that in the D group (15+/-7 microU/mL) (p<0.05). After the 100-d experimental period, blood samples were collected. The fructosamine level was significantly lower in the DM group (152+/-21 mmol/L) than in the D group (185+/-13 mmol/L). The concentration of 1.5-A.G. (1.5-anhydro glucitol) was significantly higher in the DM group (9.33+/-2.42 microg/mL) than in the D group (1.33+/-0.52 microg/mL). Observation of insulin antibody stain in the Langerhans cells of the pancreas using ABC method showed a decrease insulin antibody stain in the D group. The cells of the DM group were stained more darkly than those of the D group. From these results, we postulated that the bioactive substances present in Maitake can ameliorate the symptoms of diabetes.     

Differing effects of water-soluble and fat-soluble extracts from Japanese radish (Raphanus sativus) sprouts on carbohydrate and lipid metabolism in normal and streptozotocin-induced diabetic rats

We have shown previously that Japanese radish (Raphanus sativus) sprouts (JRS) improve blood glucose levels in diabetic rats. In this study, we investigated the components in JRS that caused this hypoglycemic effect, by examining the effects of water-soluble (WSE) and fat-soluble (FSE) extracts of JRS on diabetes markers in normal (NM) and streptozotocin (STZ)-induced diabetic (DM) rats. The NM and DM rats were divided into a control group and 2 test groups (WSE (2.2%) or FSE (0.2%)), with the rats (n = 6/group) then being maintained for 3 wk on either a control diet or one of the test diets; this was followed by the measurement of serum concentrations of glucose, insulin, glycoalbumin, fructosamine, ketone bodies, and lipids (cholesterol and triglyceride) and liver concentrations of lipids (total lipid, total cholesterol, and triglyceride). The FSE suppressed insulin secretion and improved lipid metabolism in the NM rats. The effect of WSE was different from that of the FSE as it decreased blood glucose levels without increasing insulin secretion and also lowered glycoalbumin and fructosamine levels in the DM rats. Therefore, the WSE have potential as functional food components with the hypoglycemic effect.     

蚕丝水解物对实验性糖尿病大鼠血糖的调节作用

选用若干雄性成年Ｗｉｓｔａｒ大鼠随机分为２组：糖尿病模型组大鼠尾静脉注射小剂量链脲佐菌素（ＳＴＺ,３０ｍｇ／ｋｇＢＷ）３周后,加喂高糖、高脂、高热量饲饲米。选择具有Ⅱ型糖尿病病特征即糖耐量异学、胰岛素高于或等于对照组的３８只大鼠,对照组３６只大鼠不注射ＳＴＺ,仅喂普通饲米,将糖尿病模型组再随机分为２组：糖尿病对照组、糖尿病＋蚕丝水稻物（ＳＨ）组;对照组随机分为正常对照组、正常＋ＳＨ组。每日给予ＳＨ     

仙鹤草降糖作用及对大鼠抗氧化能力影响

目的 观察仙鹤草降糖作用,并从氧化性损伤角度初步探讨其作用机制.方法 雄性SD大鼠腹腔注射链脲佐菌素建立糖尿病大鼠模型.将造模成功大鼠随机分为糖尿病模型组,仙鹤草低、高剂量组(60、120 g/kg,每天经口灌胃)及对照组.观察大鼠体重、血糖及血清胰岛素水平变化,检测血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量;做胰腺病理组织学检查.结果 模型组大鼠血清胰岛素和血糖水平分别为(7.53±2.15) μIU/mL、( 17.55±6.08) mmol/L,血清GSH-Px活性和MDA含量分别为(1110.75±268.16)μmol/mL、(6.10 +0.88) nmoL/mL;低、高剂量仙鹤草组胰岛素和GSH-Px活性分别为(16.79±6.81)、(18.29±7.15)μIU/mL和(1264.50±273.20)、(1824.38±240.59) μmol/mL,明显高于模型组(P＜0.01),血糖水平和MDA含量分别为(4.71±2.19)、(3.92±1.17) mmol/L和(4.99±0.90)、(4.92±0.50) nmol/mL,明显低于模型组(P＜0.01).结论 仙鹤草可通过增加GSH-Px活性和降低MDA含量发挥抗糖尿病作用.     

Olive leaf extract as a hypoglycemic agent in both human diabetic subjects and in rats

Olive tree (Olea europaea L.) leaves have been widely used in traditional remedies in European and Mediterranean countries as extracts, herbal teas, and powder. They contain several potentially bioactive compounds that may have hypoglycemic properties. To examine the efficacy of 500 mg oral olive leaf extract taken once daily in tablet form versus matching placebo in improving glucose homeostasis in adults with type 2 diabetes (T2DM). In this controlled clinical trial, 79 adults with T2DM were randomized to treatment with 500 mg olive leaf extract tablet taken orally once daily or matching placebo. The study duration was 14 weeks. Measures of glucose homeostasis including Hba1c and plasma insulin were measured and compared by treatment assignment. In a series of animal models, normal, streptozotocin (STZ) diabetic, and sand rats were used in the inverted sac model to determine the mechanism through which olive leaf extract affected starch digestion and absorption. In the randomized clinical trial, the subjects treated with olive leaf extract exhibited significantly lower HbA1c and fasting plasma insulin levels; however, postprandial plasma insulin levels did not differ significantly by treatment group. In the animal models, normal and STZ diabetic rats exhibited significantly reduced starch digestion and absorption after treatment with olive leaf extract compared with intestine without olive leaf treatment. Reduced digestion and absorption was observed in both the mucosal and serosal sides of the intestine. Though reduced, the decline in starch digestion and absorption did not reach statistical significance in the sand rats. Olive leaf extract is associated with improved glucose homeostasis in humans. Animal models indicate that this may be facilitated through the reduction of starch digestion and absorption. Olive leaf extract may represent an effective adjunct therapy that normalizes glucose homeostasis in individuals with diabetes.     

Lipid metabolism in the embryos of diabetic rats during early organogenesis: modulatory effect of prostaglandin E2

The purpose of this work was to evaluate de novo lipid biosynthesis and the lipid profile, and to study the effect of prostaglandin E2 (PGE2; prostaglandin has previously been found to be involved in diabetes embryopathy) on lipid metabolism in embryos from control and streptozotocin-induced diabetic rats during organogenesis. Increased levels of triacylglycerols were found in embryos of diabetic rats compared with controls, whereas no differences were detected in the levels of cholesterol, cholesterylester, phosphatidylcholine and phosphatidylethanolamine. When the de novo synthesis of lipids in the embryo was studied using [14C]acetate as a tracer, a diminished rate of incorporation of [14C]acetate into the evaluated lipid classes was detected in the diabetic embryo compared with controls. Addition of PGE2 did not modify the incorporation of [14C]acetate into any of the lipid species of control embryos, but enhanced the incorporation of [14C]acetate into triacylglycerol, cholesterylesters, phosphatidylcholine and phosphatidylethanolamine of embryos from diabetic rats. The study's results show alterations in both synthesis and concentrations of lipids in the embryos of diabetic rats. Interestingly, the results demonstrate that the addition of PGE2, a prostaglandin that reverses the embryonic morphological abnormalities induced by diabetes, prevents disturbances in embryo lipid synthesis caused by diabetes.     

Antihyperglycemic activity of Prunella vulgaris L. in streptozotocin-induced diabetic mice

Prunella vulgaris L. (Labiatae) has been reported to have a wide range of health benefits in oriental medicine. This study for the first time is to examine the antihyperglycemic effects of P. vulgaris in streptozotocin (STZ)-induced diabetic ICR mice (STZ diabetic mice). The effects of P. vulgaris L. aqueous-ethanol extract (PVE) on blood glucose, exogenous insulin sensitivity and plasma insulin levels were investigated. In four doses of extracts from the spikes of P. vulgaris, extract at dose of 100 mg/kg significantly suppressed the rise in blood glucose after 30 min in the acute glucose tolerance test. Furthermore, this dose was applied in the fellow experiments. A significant decrease in blood glucose levels was observed after treatment of PVE. A combination of PVE and glibenclamide produced a greater effect in blood glucose level than using glibenclamide or PVE alone. PVE enhanced and prolonged the antihyperglycemic effects of exogenous insulin on STZ diabetic mice. Plasma insulin levels were increased with glibenclamide treatment in STZ diabetic mice, whereas such effect was not observed with PVE. These results indicated that P. vulgaris enhances the antihyperglycemic effects of exogenous insulin without stimulating insulin secretion, indicating that insulin sensitivity is increased in STZ diabetic mice.     

Effect of vitamin D3 in reducing metabolic and oxidative stress in the liver of streptozotocin-induced diabetic rats

Diabetes mellitus is a growing health problem worldwide and is associated with severe liver complications. The aim of the present study is to analyse the status of metabolic and free-radical-scavenging enzymes and second messengers in the liver of streptozotocin (STZ)-induced diabetic rats, and to determine the hepatoprotective role of vitamin D3. All studies were performed using the liver of adult male Wistar rats. Gene expression studies were carried out using real-time PCR with specific probes. Second messenger levels were determined using 3H-labelled Biotrak assay kits, and glucose uptake assay with d-[14C]glucose. The present results show that there was a decrease in hepatic glucose uptake, malate dehydrogenase activity, glycogen content, inositol triphosphate (IP3) and cyclic GMP levels, and superoxide dismutase, glutathione peroxidase, phospholipase C, cyclic AMP-responsive element-binding protein, vitamin D receptor (VDR) and insulin receptor (INSR) gene expression in the diabetic rats when compared with the controls (all P?相似文献   

Chitosan reduces plasma adipocytokines and lipid accumulation in liver and adipose tissues and ameliorates insulin resistance in diabetic rats

Chitosan is a natural product derived from chitin. To investigate the hypoglycemic and anti-obesity effects of chitosan, male Sprague-Dawley rats were divided into four groups: normal control, diabetic, and diabetic fed 5% or 7% chitosan. Diabetes was induced in rats by injecting streptozotocin/nicotinamide. After 10 weeks of feeding, the elevated plasma glucose, tumor necrosis factor-α, and interleukin-6 and lower adiponetin levels caused by diabetes were effectively reversed by chitosan treatment. In addition, 7% chitosan feeding also elevated plasma glucagon-like peptide-1 levels and lowered the insulin resistance index (homeostasis model assessment) in diabetic rats. Lower adipocyte granular intensities and higher lipolysis rates in adipose tissues were noted in the 7% chitosan group. Moreover, chitosan feeding reduced hepatic triglyceride and cholesterol contents and increased hepatic peroxisomal proliferator-activated receptor α expression in diabetic rats. Our results indicate that long-term administration of chitosan may reduce insulin resistance through suppression of lipid accumulation in liver and adipose tissues and amelioration of chronic inflammation in diabetic rats.     

2型糖尿病大鼠模型的建立

目的 探讨2型糖尿病动物模型建立的方法.方法 根据体重将35只Wistar大鼠随机分为对照组(10只)和糖尿病组(25只);糖尿病组喂高糖高脂饲料,对照组喂普通饲料,喂养1个月后,糖尿病组大鼠一次性腹腔注射链脲佐菌素(STZ) 25 mg/kg.bw,对照组注射相同剂量柠檬酸钠缓冲液,72 h后以随机血糖≥16.7 mmol/L确定为糖尿病模型.结果 STZ注射后72 h,与对照组相比糖尿病组大鼠血糖明显升高(P＜0.05),葡萄糖耐量,胰岛素耐受均异常,血清TG,TC,LDL-C显著升高(P＜0.05),同时伴有多饮、多食、多尿等糖尿病症状.结论 高糖高脂饲料联合小剂量腹腔注射STZ可致大鼠血糖明显上升,血脂紊乱等2型糖尿病症状.     

Hypoglycemic effect of hot-water extract of adzuki (Vigna angularis) in spontaneously diabetic KK-A(y) mice

ObjectiveRecently, we reported that 40% ethanol fraction of hot-water extracts of adzuki (Vigna angularis; EtEx.40) suppressed the postprandial blood glucose level and serum insulin level in normal mice and streptozotocin-induced type 1 diabetic rats. The present study examined the hypoglycemic effect of EtEx.40 on blood glucose, insulin concentrations, organ weight, serum composition, and hepatic lipid content in spontaneously diabetic KK-A^y/Ta Jcl mice, a model for type 2 diabetes.MethodsTo investigate the prevention of type 2 diabetes by EtEx.40 ingestion, 4-wk-old non-diabetic KK-A^y mice were fed an AIN-76 diet containing 5000 mg of EtEx.40/kg of body weight per day (EtEx.40) or an AIN-76 diet without EtEx.40 for 8 wk. Furthermore, to investigate the improvement of type 2 diabetes, 7-wk-old diabetic KK-A^y mice were fed EtEx.40 for 4 wk.ResultsCompared with the control group, EtEx.40 supplementation had a significant effect in lowering blood glucose levels, water intake, serum insulin levels, urinary glucose, urinary microalbumin/creatinine ratio, liver triacylglycerol, and total cholesterol levels. Similar results were observed in 7-wk-old diabetic KK-A^y mice fed EtEx.40 for 4 wk. These effects were also found after short-term administration of EtEx40. Overall, EtEx.40 improved several diabetic symptoms in KK-A^y mice.ConclusionEtEx.40 obtained from hot-water adzuki extracts showed preventive and ameliorative effects on the progression of diabetes in genetically diabetic KK-A^y mice. In the present study, we conclude that the preventive and ameliorative effects by EtEx.40 were due to the modulation of blood glucose levels and the protective effect against oxidative damage in diabetes mellitus.