Development and characterization of microsatellite loci in Ruppia maritima L. (Ruppiaceae)

Ruppia maritima L. (Ruppiaceae), a monoecious seagrass, is widely distributed in temperate and tropical regions. In this paper, we reported ten polymorphic microsatellite loci developed for this species. The number of alleles per locus ranged from 2 to 7, and the expected heterozygosity ranged from 0.240 to 0.701. No locus pair showed significant linkage disequilibrium. These polymorphic SSR primers will be useful in population genetic studies and clone identification of R. maritima L.     

Development and characterization of microsatellite loci for Rhinogobius sp. YB (Gobiidae)

Conservation Genetics Resources - Rhinogobius sp. YB (Gobiidae) distributed in the Ryukyu Archipelago is a fluvial species of gobies. We present 34 novel microsatellite loci that were tested on 32...     

Development and characterization of ten microsatellite loci for the eastern spadefoot toad, Scaphiopus holbrookii

We isolated and characterized 10 microsatellite loci from the eastern spadefoot toad, Scaphiopus holbrookii. Loci were screened in 24 individuals from two schools of tadpoles in a single isolated wetland in South Carolina, USA. The number of alleles per locus ranged from 4 to 12, observed heterozygosity ranged from 0.200 to 0.875, and the probability of identity values ranged from 0.043 to 0.298. These new loci provide tools for examining the landscape genetics of a species facing continued destruction of its breeding habitat (small isolated wetlands) as well as fragmentation of upland life zones.     

Development and characterization of 23 microsatellite loci for scaled quail (Callipepla squamata)

The scaled quail (Callipepla squamata) is a common but declining game bird found throughout large portions of the arid southwest region of the United States. Range-wide population declines have been linked to long term drought and land use changes and have led to a resurgance in research investigating various aspects of scaled quail ecology. In order to facilitate future research on scaled quail, we have developed and characterized 23 polymorphic microsatellite loci. Among 16 individuals from populations located throughout scaled quail range, the number of alleles per locus ranged from 3 to 14 with an average of 7. Polymorphic information content ranged from 0.210 to 0.899 with an average of 0.654, indicating that these loci have high applicability for future scaled quail genetic studies.     

Novel tetranucleotide and pentanucleotide microsatellite loci in the red-cockaded woodpecker (Picoides borealis)

We describe 12 polymorphic tetranucleotide and pentanucleotide loci in the red-cockaded woodpecker (Picoides borealis). An average of 6.25 alleles per locus was identified based on a screening of 21 individuals from the Joseph W. Jones Ecological Center in southwestern Georgia. Observed heterozygosity ranged from 0.048 to 0.952. These markers could be used for both population level studies and individual identification.     

Isolation and characterization of nuclear microsatellite loci for the short-range endemic Asperula crassifolia L. (Rubiaceae)

Conservation Genetics Resources - Asperula crassifolia L. (Rubiaceae) is a narrowly endemic species whose distribution is limited to southern Italy. Data concerning relative abundance, or related...     

Isolation and characterization of nuclear microsatellite loci in the northern shrimp, Pandalus borealis

We developed and characterized 20 microsatellite primer loci for the northern shrimp Pandalus borealis. All 20 loci were polymorphic with number of alleles ranging from 3 to 36 and with observed heterozygosity between 0.04 and 0.93. In addition, we tested the utility of these markers in three related shrimp species, P. montagui, Atlantopandalus propinqvus and Dichelopandalus bonnieri. These new markers will prove useful in the identification of stock structure and hence, assessment of the commercially important species P. borealis.     

Development and characterization of 21 microsatellite markers for Pancratium maritimum L. (Amaryllidaceae)

Pancratium maritimum L. (Amaryllidaceae) is a bulbous perennial plant growing on coastal sands of Mediterranean, Black and Caspian seas as well as on part of European coasts of Atlantic Ocean. The excess of flowers sampling, urbanization and tourism put serious threats to the species, resulting in a significant decrease of its populations. In order to investigate its conservation genetics, we developed and characterized microsatellite markers in P. maritimum. Twenty-one microsatellite loci were isolated using the SSR-patchwork protocol. The average number of alleles per locus was 4.4. Polymorphic information content value ranged from 0 to 0.75 with an average of 0.39. Eleven loci showed significant departures from Hardy–Weinberg equilibrium.     

Development and characterization of tetranucleotide microsatellite loci for the American alligator (Alligator mississippiensis)

We isolated and characterized 17 tetranucleotide microsatellite loci in the American alligator, Alligator mississippiensis. Loci were screened across 27 individuals from one population and shown to be polymorphic with the number of alleles per locus ranging from 2 to 12. Polymorphic information content ranged from 0.2 to 0.85, and observed heterozygosity ranged from 0.185 to 0.889. One locus showed significant deviation from Hardy?CWeinberg equilibrium, and one pair of loci showed evidence of linkage.     

Development and characterization of microsatellite loci of Oratosquilla oratoria (Crustacea: Squillidae)

Oratosquilla oratoria (Crustacea: Squillidae) is the most dominant species in the indo-pacific ocean, which has been regarded as the marine lives for its good taste and rich nutrition in fishery. In the current study, eleven polymorphic microsatellite loci were isolated from total genome of O. oratoria, and tested their polymorphism in 30 individuals collected from the sea areas of the indo-pacific ocean in Huludao, Qingdao and Yantai. The numbers of observed alleles were from two to eleven. Moreover, the observed heterozygosity from 0.278 to 0.734 was comparable to the expected heterozygosity from 0.555 to 0.776. The microsatellite markers obtained in this study were suitable for further assessing the genetic diversity and population structure of O. oratoria.     

Development and characterization of 21 microsatellite loci for the Pine Barrens treefrog (Hyla andersonii)

Twenty-one polymorphic microsatellite loci were developed and characterized for the rare Pine Barrens treefrog (Hyla andersonii). This species is found in three disjunct regions of the eastern United States and is considered threatened by each state in which it occurs. In order to elucidate population structure and identify possible declines in this treefrog we designed primers that amplify across all three regions. Loci were isolated from a genomic library enriched for a variety of repetitive elements. The number of alleles ranged from 2 to 15, the observed heterozygosity ranged from 0.17 to 0.93, and the expected heterozygosity ranged from 0.38 to 0.95 using only 15 individuals. These microsatellite markers will address a deficiency of genetic data for H. andersonii by assessing differences among the three regions and describing population structure within each region in order to inform conservation efforts.     

Characterization of ten microsatellite loci for the dark pomfret, Pampus cinereus (Stromateidae, Perciformes)

Ten polymorphic microsatellite loci were isolated in the dark pomfret (Pampus cinereus), an important commercial foodfish species. The mean number of observed alleles per locus was 21 (range 13–33). Observed and expected heterozygosity values ranged from 0.73 to 0.98 and from 0.83 to 0.97 respectively. Two loci showed significant departure from Hardy–Weinberg equilibrium and significant linkage disequilibrium was found between P15-2 and P230. These highly informative microsatellite markers should be useful for population genetic analyses of P. cinereus and other pomfret species.     

Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus

We isolated and characterized 17 microsatellite loci from the eastern fence lizard, Sceloporus undulatus. Loci were screened in 24 individuals of S. undulatus. The number of alleles per locus ranged from 8 to 22, observed heterozygosity ranged from 0.174 to 0.913, and the probability of identity values ranged from 0.008 to 0.1. These new loci provide tools for examining the population genetics of eastern fence lizards and for assessing the evolutionary impacts the invasive red imported fire ant has on the lizard.     

Isolation and characterization of thirty microsatellite loci for Atlantic mackerel (Scomber scombrus L.)

Atlantic mackerel (Scomber scombrus L.) is a widely distributed commercially important pelagic species. Little is known about the stock structure of this species, but it is thought to be undergoing a range extension due to environmental changes. Knowledge of the stock structure under these changing conditions is fundamental for effective management. In this paper, 30 highly polymorphic microsatellite loci developed with next generation sequencing are described. The number of alleles per locus ranged from 4 to 39 in two geographically distant populations, observed and expected heterozygosities ranged between 0.370–0.978 and 0.426–0.962, respectively. These loci are an important resource that will allow assessment of the current population genetic structure of this species, and enable monitoring of climate related changes in the species range and distribution.     

Development and characterization of 29 polymorphic microsatellite loci for Esomus metallicus

Twenty-nine microsatellite loci were isolated and characterized from the genome of Esomus metallicus. Flanking polymerase chain reaction primers were designed and used to amplify these loci in 32 individuals. All loci were polymorphic; with allele numbers ranged from 2 to 27, observed heterozygosity from 0.031 to 0.969 and expected heterozygosity from 0.031 to 0.957. All conformed to Hardy?CWeinberg equilibrium and no evidence of null alleles was observed. Pairwise comparisons between alleles did not detect any cases of linkage disequilibrium. The high level of polymorphisms observed in these microsatellite loci should enhance future investigation of the genetic differentiation and structure of populations of E. metallicus.     

Development and characterization of microsatellite markers in Sarracenia L. (pitcher plant) species

Sarracenia species (pitcher plants) are carnivorous plants which obtain a portion of their nutrients from insects captured in the pitchers. Sarracenia species naturally hybridize with each other, and hybrid swarms have been identified. A number of the taxa within the genus are considered endangered. In order to facilitate evolutionary, ecological and conservation genetic analyses within the genus, we developed 25 microsatellite loci which show variability either within species or between species. Three S. purpurea populations were examined with 10 primer sets which showed within population variability.     

Development and characterization of twelve polymorphic microsatellite loci for the Chinese concave-eared frog (Odorrana tormota)

The Chinese concave-eared frog is a rare species endemic to a restricted region of easternChina. In the present study, we isolated twelve polymorphic microsatellite loci from a dinucleotide—or trinucleotide—enriched library for the frog. The number of alleles per locus ranged from 2 to 11 with an average of 5.92. The observed and expected heterozygosities varied from 0.063 to 0.862 and from 0.062 to 0.873, with an average of 0.453 and 0.601, respectively. The polymorphic microsatellite locidescribed here are expected to be useful for further studies of the genetic structure, gene flow andmating mechanism in Rana tormotus, which in turn will be helpful in developing conservation andmanagement strategies for the rare frog.     

Development and characterization of fourteen novel microsatellite loci in Chinese muntjac (Muntiacus reevesi)

Fourteen novel polymorphic microsatellite markers were isolated and characterized for Muntiacus reevesi, a small deer species. An (AG)_n enriched library was created from two individuals following the FIASCO protocol (Fast Isolation by AFLP of Sequences COtaining repeats). 14 primers were designed from 98 microsatellite sequences and tested in 32 samples. The number of alleles per locus ranged from 2 to 13 and the expected heterozygosities from 0.123 to 0.916. The loci had an average polymorphic information content value of 0.676. Five loci showed significant deviations from Hardy–Weinberg equilibrium and no linkage disequilibrium was detected. These markers should be a useful tool for further population genetic studies of Muntiacus reevesi.     

Isolation and characterization of 20 microsatellite loci for the saltmarsh plant Triglochin maritima L.

Twenty microsatellite markers were developed for the polyploid plant Triglochin maritima L., an important component of declining saltmarsh ecosystems that are now subject to much restoration effort. All loci were polymorphic when tested across 24 individuals from three populations. The average number of alleles per population was 6, ranging from 2 to 12. Private alleles were identified in each population, demonstrating the utility of these markers for the investigation of the population genetic structure and diversity of this species.