Serum adiponectin and resistin levels in patients with polycystic ovarian syndrome and their clinical implications

In this study, serum adiponectin and resistin levels were determined in 46 patients with polycystic ovarian syndrome (PCOS), and their correlation with serum sexual hormones and insulin resistance (IR) were examined.The subjects included 26 obese patients with body mass index (BMI)>25 and 20 non-obese patients with BMI≤25, with 25 obese and 25 non-obese healthy volunteers without PCOS serving as controls.Serum adiponectin and resistin levels in all subjects were measured, and endocrinal and metabolic indices were also analysed.Our results showed that the serum adiponectin levels in both obese and non-obese PCOS groups were significantly lower than their controls, while the serum resistin levels in obese and non-obese PCOS group were significantly higher than in their controls (P<0.001).The serum adiponectin level was significantly lower and serum resistin level significantly higher in the non-obese PCOS group as compared with the obese control group (P<0.05).Serum adiponectin level was negatively correlated with FIN, HOMA-IR, LH and LH/FSH (P<0.05), but serum resistin level was positively correlated with FIN, HOMA-IR, LH and LH/FSH (P<0.05).We are led to conclude that PCOS patients have obvious IR, low serum adiponectin and high serum resistin, and adiponectin and resistin might play important roles in the pathogenesis of IR in PCOS patients.     

Serum Adiponectin and Resistin Levels in Patients with Polycystic Ovarian Syndrome and Their Clinical Implicatlons

In this study, serum adiponectin and resistin levels were determined in 46 patients with polycystic ovarian syndrome (PCOS), and their correlation with serum sexual hormones and insulin resistance (IR) were examined. The subjects included 26 obese patients with body mass index (BMI)＞25 and 20 non-obese patients with BMI≤25, with 25 obese and 25 non-obese healthy volunteers without PCOS serving as controls. Serum adiponectin and resistin levels in all subjects were measured, and endocrinal and metabolic indices were also analysed. Our results showed that the serum adiponectin levels in both obese and non-obese PCOS groups were significantly lower than their controls, while the serum resistin levels in obese and non-obese PCOS group were significantly higher than in their controls (P＜0.001). The serum adiponectin level was significantly lower and serum resistin level significantly higher in the non-obese PCOS group as compared with the obese control group (P＜0.05). Serum adiponectin level was negatively correlated with FIN, HOMA-IR, LH and LH/FSH (P＜0.05), but serum resistin level was positively correlated with FIN, HOMA-IR, LH and LH/FSH (P＜0.05). We are led to conclude that PCOS patients have obvious IR, low serum adiponectin and high serum resistin, and adiponectin and resistin might play important roles in the pathogenesis of IR in PCOS patients.     

青春期多囊卵巢综合征动物模型的实验研究

目的:建立脱氢表雄酮(DHEA)诱导未成熟雌性大鼠多囊卵巢综合征(PCOS)模型,并探讨其意义。方法:用DHEA皮下注射23d龄SD雌性大鼠,观察大鼠阴道口开放日龄及开口后阴道脱落细胞形态学特征,20d后取出卵巢、肾上腺、子宫称重,并观察各器官大体解剖及光镜(HE染色)特征,检测空腹血糖(FAS),测定血清睾酮(T)、黄体生成激素(LH)、空腹胰岛素(FIN)、胰岛素样因子(IGF-1)的水平。结果:①造模组较对照组大鼠阴道口开放日龄明显提前;造模组大鼠阴道脱落细胞无周期变化;②造模组卵巢、子宫、肾上腺湿重均高于对照组;组织学造模组肾上腺皮质网状带明显增宽,子宫内膜增厚,卵巢呈多囊改变,黄体数目显著减少;③造模组FAS、FIN、T和HOMA-IR均明显高于对照组;而LH值稍高于对照组;④造模组IGF-1显著低于对照组。结论:DHEA诱导大鼠PCOS模型与青春期PCOS部分特点相似,存在肾上腺初现功能亢进的特点,可作为研究青春期PCOS理想的动物模型。     

Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats

Objective To investigate the effects of acupuncture on ovary morphology and function in dehydroepiandrosterone(DHEA)-induced polycystic ovary syndrome(PCOS)model rats.Methods A total of 40 adult female Wistar rats were randomly allocated to 4 groups by a random number table,including control,model,metformin and acupuncture groups,10 rats in each group.PCOS rat model was developed by injecting with DHEA(6 mg/100 g body weight)in 0.2 mL of oil subcutaneously.Electrical stimulation(2 Hz,3 mA)was applied to Guanyuan(CV 4),Zigong(EX-CA1)and Qihai(CV 6)acupoints for 30 min daily in the acupuncture group,and metformin(200 mg/kg)was given to rats in the metformin group,both once per day for 21 consecutive days,and rats in the normal group was fed with normal saline and fed regularly.After 21 days of administration,the rat blood samples were collected for detecting the reproductive hormonal levels[luteinizing hormone(LH),follicle stimulating hormone(FSH),estradiol(E2),progesterone(P),testosterone(T)]and inflammatory factors(visfatin,IL-6)analysis.Ovary tissue was used for histopathological analysis.Results Compared with the model group,rats in the acupuncture and metformin groups were significantly lower in weight gain,FSH,LH and T levels,and E2 and P levels significantly increased(alll P<0.05).Meanwhile,LH and FSH levels were significantly decreased,and P,T and E2 levels significantly increased in the acupuncture group,compared with the metformin group(P<0.05).Compared with the model group,IL-6 and visfatin levels were significantly decreased in the acupuncture and metformin groups(P<0.05).There were no significant differences in IL-6 and visfatin levels between the acupuncture and metformin groups(P>0.05).Ovarian diameter in the acupuncture and metformin groups were smaller than the model group(P<0.05).However,there were no significant differences in ovarian diameters between the acupuncture and metformin groups(P>0.05).Conclusion Acupuncture might improve ovary morphology and its function in DHEA-induced PCOS model rats.     

Gene Expression of Adiponectin and Adiponectin Receptor 1 in Type 2 Diabetic Rats and the Relationship with the Parameters of Glucose and Lipid Metabolism

Adiponectin is an adipocyte-derived factor ,which plays pivotal roles of the insulin-sensitizing,anti-inflammatory ,and atheroprotective . Two adi-ponectin receptor types were recently identified:AdipoR1 is abundantly expressed in muscle ,whereas AdipoR2 is predominantly expressed inthe liver[1].They are thought totransmit effects ofadiponectin . We examined serumadiponectin, mR-NAlevels of adiponectinin adipose tissue and mR-NAlevels of AdipoR1 inthe muscles of type 2 dia-betic rats ,to …     

PGC-1ɑ在多囊卵巢综合征模型大鼠卵巢组织的表达变化

  目的  探讨多囊卵巢综合征（polycystic ovary syndrome, PCOS）大鼠模型及肥胖PCOS大鼠模型卵巢组织过氧化物酶体增殖物激活受体γ辅助活化因子1α（PGC-1α）的表达,以及PCOS 发生的可能机制。  方法  SD大鼠30只,随机分为对照组、PCOS组及肥胖PCOS组,PCOS两组大鼠每日一次予脱氢表雄酮〔DHEA,60 mg/（kg·d）〕溶于0.2 mL注射用大豆油中皮下注射,共21 d,制备PCOS模型,肥胖PCOS模型组大鼠在DHEA制模基础上加入高脂饮食,每组大鼠各10只。造模前（0 d）和造模后第22天称体质量,取腹主动脉血检测血清睾酮（testosterone,T）水平,取大鼠卵巢组织,HE染色观察组织形态学改变,免疫组织化学染色和Western blot检测PGC-1ɑ蛋白表达。  结果   造模前3组大鼠体质量差异无统计学意义,造模后第22天,大鼠增加的体质量以及大鼠血清 T 质量浓度,均为肥胖PCOS组>PCOS组>对照组,差异有统计学意义（P<0.05或P<0.01）。大鼠卵巢组织HE染色结果显示,对照组大鼠卵巢组织镜下可见不同发育时期的卵泡和少量黄体,颗粒细胞排列多为4～6层;PCOS组及肥胖PCOS组大鼠卵巢组织中未成熟的小卵泡数量明显增加,颗粒细胞1～3层排列、较松散,部分卵泡闭锁,肥胖PCOS组大鼠卵巢闭锁卵泡直径增大,颗粒细胞层数减少,卵母细胞消失等现象更明显。免疫组化染色结果显示,对照组大鼠卵巢组织PGC-1ɑ蛋白阳性表达主要分布于卵丘及颗粒细胞。从PGC-1ɑ蛋白在卵巢组织的表达平均灰度均值来看,PCOS组（0.53±0.06）和肥胖PCOS组（0.36±0.03）均低于对照组（0.75±0.03）,差异有统计学意义（P<0.05）,肥胖PCOS组PGC-1ɑ表达低于PCOS组（P<0.01）。Western blot检测结果与免疫组化染色结果一致。  结论  PGC-1ɑ表达降低与高脂环境下卵巢颗粒细胞损伤有关。PGC-1ɑ在卵巢卵泡的颗粒细胞中表达降低可能是PCOS发生发展的重要原因。     

妊娠期大鼠大量摄入酒精对新生鼠血糖的影响及其机制

目的：观察孕期摄入酒精对新生鼠血糖的影响并探讨其发生机制。方法：6只Wistar孕鼠随机分为酒精灌胃组（4g•kg^-1•d^-1至分娩前）和对照组（给予等容积蒸馏水）。分娩后每组随机选12只新生鼠,摄入酒精孕鼠的新生鼠为酒精组,给予蒸馏水孕鼠的新生鼠为对照组。RT-PCR检测新生鼠脂肪组织瘦素、抵抗素mRNA表达水平,形态测定法观察胰岛细胞形态并分析胰岛结构参数。结果：与对照组比较,酒精灌胃组的新生鼠出生时体重明显降低（P<0.001）,血糖升高（P<0.05）,血浆及胰腺胰岛素含量均显著降低（P<0.05）。酒精灌胃组新生鼠脂肪组织中抵抗素mRNA表达量增加（P<0.05）,而瘦素mRNA表达量降低（P<0.05）。酒精灌胃组新生鼠胰岛及 细胞形态未发生改变,胰岛数量及 细胞密度与对照组比较差异无显著性 （P>0.05）。结论：孕鼠大量摄入酒精引起新生鼠胰岛素分泌减少,改变与胰岛素抵抗相关的脂源性细胞因子基因表达,从而使新生鼠血糖升高,亦可能是引起成年期胰岛素抵抗发生的原因之一。     

大豆异黄酮对胰岛素抵抗大鼠抵抗素基因表达的影响

目的探讨大豆异黄酮(SIF)改善高脂高蔗糖膳食诱导大鼠胰岛素抵抗(IR)状态的作用及可能机制。方法选用高脂高蔗糖饲料诱导的IR雄性SD大鼠,根据胰岛素抵抗指数(IRI)随机分为模型对照组和3个SIF组(50mg/kg·bw、150mg/kg·bw及450mg/kg·bw)。各组给予相应受试物1月后,酶法检测各组动物空腹血糖、放免法检测空腹血胰岛素、酶免法检测血抵抗素含量,实时定量RT-PCR法检测肾周脂肪组织抵抗素基因的mRNA水平。结果与模型对照组比较,450mg/kg·bwSIF组能明显降低大鼠肾周脂肪组织抵抗素基因mRNA的表达及血清抵抗素的含量。150mg/kg·bwSIF和450mg/kg·bwSIF组能显著降低空腹血胰岛素水平及IRI。3个SIF组的血糖与对照组比较差异无统计学意义。IRI与血清抵抗素水平存在着明显的正相关(r=0.355,P<0.05)。结论SIF可能具有通过下调抵抗素基因mRNA表达及降低血清含量而提高胰岛素敏感性的作用。     

Effect of ARB on Expression of CD68 and MCP-1 in Adipose Tissue of Rats on Long-term High Fat Diet

In adipose tissue of rats on long-term high fat diet, the inflammatory changes the roles of angiotensin receptor blocker （ARB） in pimelitis and insulin resistance （IR） were observed. LR rat model was established by feeding high calorie and high fat diet. The change in insulin sensitivity was detected by euglycemic-hyperinsulinemic clamp technique 8 weeks after intervention by valsartan. The expression levels of CD68 and MCP-1 mRNA and proteins in adipose tissue were examined by RT-PCR and immunohistochemistry respectively. The parameters of blood glucose, insulin and blood lipid were analyzed. The results showed that in high fat diet group intra-abdominal obesity developed the content of visceral fat and the number of inflammatory cells in local adipose tissue were significantly increased （P〈0.01）, the levels of serum triglyceride, free fatty acids and fasting serum insulin were markedly increased, the insulin sensitivity was significantly lowered （P〈0.01）, and the expression of CD68 and MCP-1 was significantly increased as compared with control group （P〈0.01）. In ARB interventional group, the content of visceral fat, the number of inflammatory cells and the ex- pression of CD68 and MCP-1 in local adipose tissue were significantly reduced （all P〈0.01）, but the insulin sensitivity was significantly enhanced （P〈0.01） as compared with high fat diet group. There were pimelitis and IR in rats with obesity induced by long-term high calorie and high fat diet. The ARB can significantly inhibit the infiltration of macrophages and the expression of MCP-1 in adipose tissue, thereby attenuating the inflammation and improving LR in rats.     

维吾尔族肥胖伴胰岛素抵抗患者内脂素的表达变化及意义

目的探讨维吾尔族肥胖(OB)伴胰岛素抵抗(IR)患者血清内脂素的变化及意义。方法根据胰岛素抵抗诊断标准(胰岛素抵抗指数≥2.8),将患者分为肥胖伴胰岛素抵抗组(OB伴IR组)31例,肥胖无胰岛素抵抗组(OB无IR组)26例,另选14例健康体检者为正常对照组。半定量RT-PCR方法检查研究对象网膜和皮下脂肪组织内脂素mRNA的表达水平。对所有受试者采用稳态模式评估法(HOMA)评价胰岛素抵抗(IR),采用ELISA法测定血清内脂素水平,同时进行临床生化参数的检测。结果(1)OB伴IR组血清内脂素水平高于OB无IR组,差异有统计学意义(P<0.05)。(2)血清内脂素与体质量、甘油三酯(TG)呈正相关,与高密度脂蛋白-胆固醇(HDL-C)呈负相关。(3)3组网膜与皮下脂肪组织内脂素mRNA表达水平差异无统计学意义。结论血清内脂素水平的升高可能与维吾尔族肥胖患者的胰岛素抵抗有关。     

Wistar大鼠胰岛素抵抗PCOS动物模型的构建及其特征分析

目的建立脱氢表雄酮（DHEA）诱导的大鼠多囊卵巢综合征（polycystic ovarian syndrome,PCOS）动物模型,并探讨其胰岛素抵抗（insulin resistance,IR）特征。方法将21日龄Wistar雌性大鼠随机分为PCOS组和对照组（每组20只）,PCOS组每日皮下注射DHEA6 mg/100 g加0.2ml大豆油剂,连续20 d;对照组每日皮下注射0.2 ml大豆油剂,连续20 d。观察卵巢形态学改变,测定血中性激素、胰岛素样生长因子-1（IGF-1）、转化生长因子β1（TGFβ1）、空腹血糖（FPG）和空腹胰岛素（FINS）浓度,并计算胰岛素抵抗指数（HOMA1-IR）。结果 PCOS组卵巢囊性扩张卵泡和闭锁卵泡增多,发育卵泡减少;卵泡膜细胞增生。与对照组比较,PCOS组卵巢重量增加（P〈0.05）,血清睾酮（T）升高（P〈0.01）,FPG、FINS和HOMA1-IR均显著增高（P〈0.05或P〈0.01）。结论 DHEA可成功诱导Wistar大鼠胰岛素抵抗PCOS模型。     

Expression of Resistin Protein in Normal Human Subcutaneous Adipose Tissue and Pregnant Women Subcutaneous Adipose Tissue and Placenta

Resistinisanovelpolypeptidehormoneintheadiposetissue,whichcharacterizedbyacysteine richmotif(CX12CX8CXCX3CX10CXCXCX9CC).Resistinismainlyexpressedinthewhiteadiposetissueandweakinthebrownadiposetissue.Re sistinlinksobesitytodiabetes[1,2].Serumresistinlevelsismarkedlyincreasedinob/obanddb/dbmouse[1].Thiazolidinedionescanobviouslyreduceresistinexpressioninmouse.Duringthepregnan cy,pregnantwomenhavephysiologicalinsulinre sistance,decreaseglucosetoleranceandinsulinsensitivityandareeasytoget…     

芪蛭降糖胶囊对糖尿病大鼠胰岛素抵抗的作用及其机制

目的：探讨芪蛭降糖胶囊(QJ)对糖尿病大鼠胰岛素抵抗(IR)的作用,阐明其药物作用的分子药理机制。方法：100只Wistar大鼠采用高脂饮食联合注射链脲佐菌素（STZ）的方法复制2型糖尿病大鼠模型。将建模成功大鼠随机分为模型组（DM）,参芪降糖颗粒阳性对照组（SQ）,芪蛭降糖胶囊低（QJL）、中 (QJM)和高剂量组(QJH),同时设立正常对照组（NC）。芪蛭降糖胶囊低、中和高剂量组药物浓度分别为0.34、0.68和1.35 g?kg-1;参芪降糖颗粒药物浓度为0.27 g?kg-1。大鼠建模成功后,给予相应浓度药物干预治疗8周。经药物干预后,应用血糖检测仪及全自动生化分析仪测定大鼠空腹血糖（FBG）、空腹胰岛素（FINS）、胰岛素抵抗指数（IRI）和脂质代谢相关生化指标,Real Time PCR法测定肝脏组织中胰岛素受体底物1（IRS-1）、磷脂酰肌醇-3激酶（PI3K）和葡萄糖转运体4（GLUT4）基因的mRNA表达水平,ELISA法测定血清肿瘤坏死因子α（TNF-α）和脂联素（ADPN）水平。结果：与正常对照组比较,模型组大鼠FBG、FINS和IRI显著升高（P<0.05或P<0.01）;血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白（LDL）水平显著升高（P<0.05）,血清高密度脂蛋白（HDL）水平显著降低（P<0.05）;肝脏组织中IRS-1、PI3K和GLUT4基因的mRNA表达水平显著下降（P<0.05）,血清TNF-α水平显著升高（P<0.05）,血清ADPN水平显著降低（P<0.05）。与模型组比较,芪蛭降糖胶囊低、中、高剂量组及参芪降糖颗粒阳性对照组大鼠FBG和IRI显著降低（P<0.01）;芪蛭降糖胶囊中、高剂量组及参芪降糖颗粒阳性对照组FINS显著降低（P<0.05）;芪蛭降糖胶囊中剂量组及参芪降糖颗粒阳性对照组血清TC、TG和LDL水平显著降低（P<0.05或P<0.01）,HDL水平显著升高（P< 0.05）;肝脏组织中IRS-1、PI3K和GLUT4基因的mRNA表达水平显著上调（P<0.05）;芪蛭降糖胶囊中、高剂量组及参芪降糖颗粒阳性对照组血清TNF-α水平显著降低（P<0.05）,血清ADPN水平显著升高（P<0.05）。结论：芪蛭降糖胶囊具有改善糖尿病大鼠IR的作用,其作用机制与改善糖脂代谢、影响胰岛素信号传导通路有关。     

大豆异黄酮对胰岛素抵抗大鼠低度炎症因子水平的影响

目的探讨大豆异黄酮(SIF)对膳食诱导的胰岛素抵抗大鼠低度炎症介质水平的影响,以初步阐明大豆异黄酮改善肥胖性胰岛素抵抗状态的可能机制。方法选用高脂饲料诱导的IR雄性SD大鼠,随机分为模型对照组和3个SIF组(50、150及450mg/kg·b.w.)。各组给予相应受试物1月,禁食过夜后股动脉采血处死各大鼠,分离肾周及睾周白色脂肪。酶法检测各组动物空腹血糖,放免法检测空腹血胰岛素IL-6及TNF-α,酶免法检测血清C-反应蛋白、抵抗素与脂联素含量。结果与模型对照组比较:150mg/kg·b.w.与450mg/kg·b.w.组的内脏脂肪/体质量比、血清胰岛素、胰岛素抵抗指数TNF-α及抵抗素含量明显降低;450mg/kg·b.w.组能明显降低血清IL-6及提高脂联素的含量;3个SIF组的C-反应蛋白水平与模型组比较无明显差异。结论SIF具有提高胰岛素抵抗大鼠胰岛素敏感性的作用,可能是通过减少大鼠体内脂肪沉积、调整脂肪源低度炎症介质的分泌而实现的。     

补肾促排卵汤对多囊卵巢综合征模型大鼠卵巢形态及性激素的影响

目的通过观察补肾促排卵汤对多囊卵巢综合征模型大鼠的性激素、抗苗勒氏管激素(antiMüllerian hormone,AMH)、卵巢形态的影响,探讨补肾促排卵汤治疗多囊卵巢综合征(polycystic ovary syndrome,PCOS)的作用机制。方法应用补肾促排卵汤作用于以脱氢表雄酮诱导的PCOS大鼠模型,对卵巢进行形态学分析并检测血清性激素、AMH。结果与正常组相比,模型组大鼠卵巢弥漫性囊性重度扩张,可见大小不等的囊腔,粒层细胞被挤压呈扁平或立方形,黄体及各种发育阶段卵泡稀少。补肾促排卵汤能明显改善PCOS模型大鼠成熟卵泡个数、黄体个数、性激素及AMH水平,与模型组比较差异均有统计学意义(P0.05)。结论补肾促排卵汤能改善PCOS模型大鼠血清性激素水平、降低血清AMH及恢复卵巢排卵功能,从而达到治疗多囊卵巢综合征的作用。     

通络方剂对1型糖尿病大鼠糖脂代谢的影响

目的探讨通络方剂对1型糖尿病大鼠糖脂代谢的影响。方法 30只成年雄性SD大鼠随机分为正常对照组(NC)、糖尿病组(DM)、通络方剂治疗组(TLR),每组10只;后两组大鼠采用链脲佐菌素(STZ)60 mg/kg腹腔注射制备1型糖尿病模型。模型制备成功后,通络方剂组给予通络方剂(1.0 g.kg-1.d-1)灌胃,糖尿病组、正常对照组给予同剂量双蒸水灌胃。8周后比较各组大鼠体质量、附睾周围脂肪组织质量、空腹血糖(FBG)、空腹胰岛素(FINS)及血总胆固醇(TC)、三酰甘油(TG)、游离脂肪酸(FFA)、瘦素(leptin)水平,检测脂肪组织中瘦素mRNA表达水平。结果与糖尿病组相比,通络方剂治疗组大鼠体质量增加(P<0.01)、血FBG降低(P<0.05)、FINS升高(P<0.01)、FFA降低(P<0.01)、血leptin水平增加(P<0.05);脂肪组织leptin mRNA表达水平升高(P<0.05)。结论通络方剂可能通过改善糖尿病大鼠瘦素、胰岛素低水平状态纠正糖脂代谢紊乱。     

PPARγ激动剂对糖尿病大鼠载脂蛋白M分泌和表达的影响

目的:研究高选择性过氧化物酶增殖体激活型受体γ(PPARγ)激动剂罗格列酮(rosiglitazone,RSG)对糖尿病大鼠血清载脂蛋白M(apoM)水平和肝、肾、脂肪组织apoM mRNA表达的影响。方法:雄性SD大鼠分为对照组(Con组,n=7)、高脂组(HF组,n=8)、糖尿病组(DM组,n=7)和糖尿病RSG干预组(RSG组,n=7)4组。实验模型建立前采血检测各组大鼠空腹血糖(FBG)、空腹胰岛素(FINS)、三酰甘油(TG)、总胆固醇(TC)。糖尿病大鼠模型的建立参照周氏等方法,给予高脂高糖饲料喂养、腹腔注射链脲佐菌素。应用RSG对糖尿病大鼠干预治疗8周。全部动物于实验第15周结束时集中处死,搜集血标本和肝、肾、脂肪组织标本。检测各组大鼠的血FBG,FINS,TG,TC浓度。ELISA检测各组大鼠的血apoM水平,并与血FBG,FINS,TG,TC水平进行相关分析。RT-PCR测定各组大鼠肝、肾、脂肪组织apoM mRNA的表达。结果:与Con组和HF组比较,DM组大鼠血清apoM水平明显降低(P<0.05),RSG组大鼠的血清apoM水平较DM组明显升高(P<0.05)。各组大鼠以肝组织apoM mRNA的表达最高,肾组织次之,脂肪组织的表达最低。与Con组比较,HF组、DM组和RSG组的大鼠肝、肾、脂肪组织apoM mRNA的表达明显减少(P<0.05),RSG组大鼠肝、肾、脂肪组织apoM mRNA的表达较DM组均有明显增加(P<0.05)。大鼠血清apoM水平与血清TG(r=–0.466,P=0.011),TC(r=–0.568,P=0.001),FBG(r=–0.371,P<0.001),FINS(r=–0.768,P=0.048)水平均呈显著负相关。结论:ApoM在血糖和血脂的代谢中起着重要作用并受PPARγ激动剂调节的影响。     

炔雌醇环丙孕酮片联合二甲双胍对多囊卵巢综合征 患者血清DHEA、AMH 及SHBG 水平的影响

目的 探讨多囊卵巢综合征（PCOS）患者血清硫酸脱氢表雄酮（DHEA）、抗缪勒管激素（AMH）、 性激素结合球蛋白（SHBG）水平及经炔雌醇环丙孕酮片联合二甲双胍治疗后的变化。方法 选取2018 年 12 月—2019 年10 月在重庆三峡中心医院妇产科就诊的PCOS 患者52 例作为观察组。另取同期有规律月经 周期、卵巢功能正常的育龄期女性55 例作为对照组。观察组应用炔雌醇环丙孕酮片联合二甲双胍治疗,疗程 3 个月。比较两组治疗前血清DHEA、AMH 及SHBG 水平和观察组治疗前后血清DHEA、AMH 及SHBG 水平。采用Pearson 法分析血清DHEA、AMH、SHBG 与PCOS 病情严重程度的关系。结果 观察组治疗前 DHEA、AMH 较对照组高（P <0.05）,SHBG 较对照组低（P <0.05）。1 型组治疗前血清DHEA、AMH 水平 高于3 型组、4 型组（P <0.05）,SHBG 水平低于3 型组、4 型组（P <0.05）,2 型组患者治疗前血清DHEA、 AMH 水平高于4 型组（P <0.05）,SHBG 水平低于4 型组（P <0.05）。血清DHEA、AMH 与PCOS 病情严 重程度呈正相关（r =0.827 和0.764,P <0.05）,SHBG 与PCOS 病情严重程度呈负相关（r =-0.852,P <0.05）。 观察组治疗后血清DHEA、AMH 较治疗前低（P <0.05）,SHBG 较治疗前高（P <0.05）。结论 PCOS 患者 血清DHEA、AMH 水平升高,SHBG 水平降低。血清DHEA、AMH 及SHBG 均能有效反映PCOS 患者的病 情严重程度。炔雌醇环丙孕酮片联合二甲双胍对PCOS 患者具有良好的疗效,能有效改善患者血清DHEA、 AMH 及SHBG 水平。     

IRS-1 IRS-2蛋白质在PCOS患者脂肪组织中的表达

目的研究多囊卵巢综合征(Polycystic ovary syndrome,PCOS)患者脂肪组织胰岛素受体底物-1(Insulin receptor substeate-1,IRS-1)及胰岛素受体底物-2(Insulin receptor substeate-2,IRS-2)蛋白的表达,探讨PCOS产生胰岛素抵抗(Insulin resistance,IR)的分子机制。方法采用放射免疫法检测PCOSIR组(20例)、PCOS非IR组(15例)及对照组(20例)血清空腹胰岛素(Fasting insulin,FIN)的浓度;采用葡萄糖氧化酶法测定血浆空腹血糖(Fasting plasma glucose,FPG);采用HOMA(Homeostasis model assessment,HOMA)模型计算胰岛素抵抗指数(HOMA-IR);采用Western blot方法检测IRS-1及IRS-2蛋白的表达。结果(1)PCOS IR组患者血清FIN及HOMA-IR均显著高于PCOS非IR组与对照组(均P<0.05);PCOS非IR组患者血清FIN及HOMA-IR亦显著高于对照组(均P<05);(2)PCOSIR组与PCOS非IR组及对照组相比,IRS-1蛋白表达量明显下降(P<0.05),而IRS-2蛋白表达无显著性差别。结论PCOS患者脂肪组织IRS-1蛋白表达的下降所导致的受体后信号转导障碍可能是其产生胰岛素抵抗的机制之一。