RP—HPLC法测定杜仲中松脂醇二葡萄糖苷的含量

杜仲（ＥｕｃｏｍｍｉａｕｌｍｏｉｄｅｓＯｌｉｖ．）有降低血压，补肾安胎的功效，主要成分为杜仲胶、氯原酸、桃叶珊瑚苷等。Ｃｈａｒｌｅｓ从中国产杜仲中分离得到了松脂醇二葡萄糖苷（ｐｉｎｏｒｅｓｉｎｄｄｉｇｌｕｃｏｓｉｄｅ），并确证了其为杜仲降压的有效成分［１］，同时向多个国家申请了专利。唯一明确的松脂醇二葡萄糖苷的含量测定法有正相高效液相色谱法［２］，我们在药典标准制订过程中，采用了反相高效液相法测定其含量，流动相为甲醇－水（２５∶７５），方法简便，松脂醇二葡萄糖苷能得到较好的分离。１　仪器与试药Ｓ…     

女贞子化学成分的研究(Ⅱ)

继前报，作者又从女贞子（ＬｉｇｕｓｌｒｕｍＬｕｃｉｄｕｍＡｉｌ．）乙醇提取物的石油醚萃取部分和乙酸乙酯萃取部分分离鉴定了４个化合物．化合物Ⅵ为１９α－羟基－３－乙酰乌索酸（１９α－ｈｙｄｒｏｘｙ－３－ａｃｅｔｙｌ－ｕｒｓｏｌｉｃａｃｉｄ），化合物Ⅸ为对羟基苯乙醇－β－Ｄ－葡萄糖甙（ｐ－ｈｙｄｒｏｘｙｐｈｅｎｅｔｈｙｌ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ），化合物Ｘ为芹菜素－７－Ｏ－β－Ｄ－葡萄糖甙（ｃｏｓｍｏｓｓｉｎ），化合物Ⅺ为甘露醇（ｍａｎｎｉｔｏｌ）．其中化合物Ⅵ为一新天然产物．     

翼核果中化学成分的研究Ⅰ

本文从广西产翼核果（ＶｅｎｔｉｌａｇｏｌｅｉｏｃａｒｐａＢｅｎｔｈ．）根茎的抗炎有效部位碱提取酸沉淀中分离得到了６个化合物，其中４个蒽醌类化合物：大黄素（１），大黄素－８－Ｏ－β－Ｄ－葡萄糖甙（ｅｍｏｄｉｎ－８－Ｏ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ）（２），大黄素－６，８－二甲醚（ｅｍｏｄｉｎ－６，８－ｄｉｍｅｔｈｙｌｅｔｈｅｒ）（３），１－羟基蒽醌（１－ｈｙｄｒｏｘｙａｎｔｈｒａｑｕｉｎｏｎｅ）（４），１个酮类化合物：１，６－二羟基－３－甲基酮－８－羧酸（ｃａｌｙｘａｎｔｈｏｎｅ）（５），及１个异黄酮类化合物：鸢尾甙元（ｔｅｃｔｏｒｉｇｅｎｉｎ）（６）．其中化合物（２），（５）为本种植物中首次分离得到，（６）为本属植物中首次分离得到。     

翼核果中化学成分的研究Ⅰ

本文从广西产翼核果（ＶｅｎｔｉｌａｇｏｌｅｉｏｃａｒｐａＢｅｎｔｈ．）根茎的抗炎有效部位碱提取酸沉淀中分离得到了６个化合物，其中４个蒽醌类化合物：大黄素（１），大黄素－８－Ｏ－β－Ｄ－葡萄糖甙（ｅｍｏｄｉｎ－８－Ｏ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ）（２），大黄素－６，８－二甲醚（ｅｍｏｄｉｎ－６，８－ｄｉｍｅｔｈｙｌｅｔｈｅｒ）（３），１－羟基蒽醌（１－ｈｙｄｒｏｘｙａｎｔｈｒａｑｕｉｎｏｎｅ）（４），１个酮类化合物：１，６－二羟基－３－甲基酮－８－羧酸（ｃａｌｙｘａｎｔｈｏｎｅ）（５），及１个异黄酮类化合物：鸢尾甙元（ｔｅｃｔｏｒｉｇｅｎｉｎ）（６）．其中化合物（２），（５）为本种植物中首次分离得到，（６）为本属植物中首次分离得到。     

连翘中一个新木脂素单糖甙

从连翘果实中分离得到了五个单体化合物，根据理化性质和光谱分析鉴定为：对－羟基苯乙酸[p-hydroxyphenyl acetic acid](1),( )松脂素－β-D－葡萄糖甙[( )pinoresinol-β-D-glucoside]（2），（＋）表松脂素－4－β-D-葡萄糖甙[( )epipinoresinol-4-β-D-glucoside]（3），（＋）松脂素单甲基醚－β-D-葡萄糖甙[( )pinoresinol mono methyl ether-β-D-glucoside]（4），连翘甙[phillyrin](5)。其中3未见文献报告，1－4为首次从连翘中分离得到的已知成分。     

知母中三个新的呋甾皂苷

从中药知母（ＡｎｅｍａｒｒｈｅｎａａｓｐｈｏｄｅｌｏｉｄｅｓＢｇｅ．）中分离出三种新的呋甾皂苷，初步鉴定为（２５Ｓ）－２６－Ｏ－β－Ｄ－葡萄糖－５β－呋甾－２０（２２）－双键－３β，２６－二醇－３－Ｏ－β－Ｄ－葡萄糖基（１→２）〔β－Ｄ－葡萄糖基（１→３）〕－β－Ｄ－葡萄糖基（１→４）－β－Ｄ－半乳糖苷（１），（２５Ｓ）－２６－Ｏ－β－Ｄ－葡萄糖基－２２－羟基－５β－呋甾－３β，２６－二醇－３－Ｏ－β－Ｄ－葡萄糖基（１→２）〔β－Ｄ－葡萄糖基（１→３）〕－β－Ｄ－葡萄糖基（１→４）－β－Ｄ－半乳糖苷（２），（２５Ｓ）－２６－Ｏ－β－Ｄ－葡萄糖基－２２－甲氧基－５β－呋甾－３β，２６－二醇－３－Ｏ－β－Ｄ－葡萄糖基（１→２）〔β－Ｄ－葡萄糖基（１→３）〕－β－Ｄ－葡萄糖基（１→４）－β－Ｄ－半乳糖苷（３）．分别命名为ｔｉｍｏｓａｐｏｎｉｎ－ＢⅣ，ｔｉｍｏ－ｓａｐｏｎｉｎ－ＢⅤ，ｔｉｍｏｓａｐｏｎｉｎ－ＢⅥ．     

人字草黄酮类化学成分研究

从豆科植物人字草中分得５个黄酮类化合物，理人性质和光谱数据分析结果，证明其化学结果分别为芹菜素（Ａｐｉｇｅｎｉｎ），槲皮素（Ｑｕｅｒｃｅｔｉｎ），芹菜素－７－Ｏ－β－Ｄ葡萄糖甙（Ａｐｉｇｅｎｉｎ－７－Ｏ－β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｉｄｅ）山奈酚－３－Ｏ－β－Ｄ葡萄糖甙（Ｋａｅｍｐｆｅｒｏｌ－３－Ｏ－β－ｇｌｕｃｏｐｙｒａｎｏｓｉｄｅ）和芹菜素７－Ｏ－新橙皮糖甙（Ａｐｉｇｅｎｉｎ－７－Ｏ－ｎｅ     

女贞子化学成分的研究（Ⅱ）1）

继前报，作者又从女贞子（Ｌｉｇｕｓｔｒｕｍ Ｌｕｃｉｄｕｍ Ａｉｔ。）乙醇提取物的石油醚萃取部分和乙酸乙酯萃取部分分离鉴定了４个化合物。化合物Ⅵ为１９ａ－羟基－３－乙酰乌索酸（１９ａ－ｈｙｄｒｏｘｙ－３－ａｃｅｔｙｌ－ｕｒｓｏｌｉｃ ａｃｉｄ），化合物Ⅸ为对羟基苯乙醇－β－Ｄ－葡萄糖甙（ｐ－ｈｙｄｒｏｘｙｐｈｅｎｅｔｈｙｌ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ），化合物Ｘ为芹菜素－７－Ｏ－β－Ｄ－葡萄糖甙（     

早开堇菜的有效成分(英文)

首次报道了自堇菜科植物早开堇菜（ＶｉｏｌａｐｒｉｏｎａｎｔｈａＢｇｅ．）全草中分离到三个香豆素类化合物。根据理化性质与光谱数据（ＩＲ．ＭＳ．１ＨＮＭＲ．１３ＣＮＭＲ．２ＤＮＭＲ）推定，化合物Ⅲ的结构为６－羟基，７－［（６－Ｏ－乙酰基－β－Ｄ－葡萄糖基）－Ｏ－］－香豆素［６－ｈｙｄｒｏｘｙ，７－［（６’－Ｏ－ａｃｅｔｙｌ－β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｙｌ）－ｏｘｙ－］－ｃｏｕｍａｒｉｎ］是新化合物，命名为早开堇菜甙（ｐｒｉｏｎａｎｔｈｏｓｉｄｅ）。化合物Ⅰ、Ⅱ分别为已知化合物七叶内酯（ｅｓｃｕｌｅｔｉｎ）和菊艺甙（ｃｉｃｈｏｒｉｉｎ）。     

1-(2,6-二甲基苯氧基)-2-(3,4-二甲氧基苯乙氨基)丙烷盐酸盐对内皮素促血管平滑肌细胞增殖及癌基因表达的影响

采用内皮素－１（ＥＴ－１０．１μｍｏｌ·Ｌ－１）建立培养的血管平滑肌细胞增殖模型，用［３Ｈ］胸腺嘧啶核苷（［３Ｈ］ＴｄＲ）参入法，流式细胞术，免疫细胞化学及Ｎｏｒｔｈｅｒｎｂｌｏｔ方法，观察了１－（２，６－二甲基苯氧基）－２－（３，４－二甲氧基苯乙氨基）丙烷盐酸盐（ＤＤＰＨ０．１μｍｏｌ·Ｌ－１）对血管平滑肌细胞增殖的作用及对原癌基因及抑癌基因的影响．结果发现：ＤＤＰＨ能逆转ＥＴ－１所致［３Ｈ］ＴｄＲ参入量增多，阻止血管平滑肌细胞由静止期（Ｇ０／Ｇ１期）进入ＤＮＡ合成期（Ｓ期）和有丝分裂期（Ｇ２／Ｍ期），并能逆转ＥＴ－１引起的ｃ－ｆｏｓ，ｃ－ｍｙｃ，ｃ－ｓｉｓ原癌基因相关抗原及ｍＲＮＡ表达增强，Ｐ５３抑癌基因相关抗原及ｍＲＮＡ表达减弱．提示ＤＤＰＨ能抑制血管平滑肌细胞增殖，与癌基因调控的分子生物学机理有关．     

知母中新的甾体皂苷

从中药知母（ＡｎｅｍａｒｈｅｎａａｓｐｈｏｄｅｌｏｉｄｅｓＢｇｅ．）中分离出一种新的呋甾皂苷，用化学和光谱方法确定其结构为：（２５Ｓ） ２６ Ｏ β Ｄ 吡喃葡萄糖基 ５β 呋甾 ２０（２２） 烯 ２β，３β ２６ 三醇 ３ Ｏ β Ｄ 吡喃葡萄糖基（１→２） β Ｄ 吡喃半乳糖苷，命名为知母皂苷Ｄ．     

翼核果中两个微量新化合物及其生物活性

从翼核果的茎中分离得到两个微量新化合物，分别为１，６ 二羟基 ３ 甲基口山酮 ８ 羧基 １ Ｏ β Ｄ 吡喃葡萄糖苷（Ⅰ）及１，６ 二羟基 ３ 甲基口山酮 ８ 羧基 １ Ｏ β Ｄ 吡喃葡萄糖基（１→６） β Ｄ 吡喃葡萄糖苷（Ⅱ）；命名为翼核口山酮苷及翼核口山酮二糖苷．测定了这两个化合物对人慢性髓样白血病细胞Ｋ５６２的抑制生长作用．     

加拿大产西洋参茎叶中的一个新三萜皂苷

从加拿大产西洋参（ＰａｎａｘｑｕｉｎｑｕｅｆｏｌｉｕｍＬ．）的茎叶中分离得到一种新达玛烷型三萜皂苷，利用理化性质和光谱数据，鉴定为３－Ｏ－〔β－Ｄ－吡喃葡萄糖基－（１－２）－β－Ｄ－吡喃葡萄糖基〕－１２－羟基达玛－２３，２５－二烯－２０－β－Ｄ－吡喃葡萄糖苷，命名为西洋参皂苷Ｌ１．     

中药黄独的化学成分

目的对从中药黄独 (DioscoreabulbiferaL .)乙醇 (w =0 75 )提取物的乙酸乙酯萃取部分分离得到 5个化合物进行结构鉴定。方法采用硅胶柱色谱、高效液相色谱法进行分离 ,利用理化性质和谱学分析 ,鉴定分离得到的化合物结构。结果从中分得的 5个化合物其结构鉴定为 3,5 ,3′ 三甲氧基槲皮素 ( 3,5 ,3′ trimethoxyquercetin ,Ⅰ )、山奈酚 3 O β D 吡喃半乳糖苷 (kaempferol 3 O β D galactopyranoside ,Ⅱ )、香草酸 (vanillicacid ,Ⅲ )、异香草酸 (isovanillicacid ,Ⅳ )及 ( + )表儿茶素( ( + )epicatechin ,Ⅴ )。结论化合物Ⅰ、Ⅱ为首次从薯蓣属植物中分得的已知化合物 ,Ⅲ、Ⅳ为首次从黄独中分离得到的已知化合物     

New sesquiterpene and phenolic glucosides from Saussurea involucrata

One new guaiane-type sesquiterpene glucoside (1) and one new phenolic glucoside (2) were isolated from the whole herb of Saussurea involucrata. Their structures were established by spectroscopic methods, mainly 1D and 2D NMR, and mass spectral analysis.     

Polyphenols from plants used in traditional Indonesian medicine (Jamu): uptake and antioxidative effects in rat H4IIE hepatoma cells

Phytochemical investigation of plants used in traditional Indonesian medicine (Jamu) yielded lignans (pinoresinol, 9 alpha-hydroxypinoresinol and salicifoliol), flavonoids (3-O-beta-(D)-glucopyranosyl-(1-->6)-beta-(D)-glucopyranosylkaempferol, luteolin and apigenin) and coumarins (coumarin, 8-hydroxycoumarin and 5-hydroxycoumarin). The beneficial effects of the respective plants for human health are thought to be associated with antioxidative activity. In the present study, the antioxidative capacity of the isolated compounds was determined in an in-vitro assay. Luteolin and kaempferol (cleavage product of 3-O-beta-(D)-glucopyranosyl-(1-->6)-beta-(D)-glucopyranosylkaempferol, which is thought to be formed in the intestine) showed strong antioxidant activity; pinoresinol and 9 alpha-hydroxypinoresinol showed only minor antioxidative effects. The coumarins, as well as apigenin and 3-O-beta-(D)-glucopyranosyl-(1-->6)-beta-(D)-glucopyranosylkaempferol were inactive. The antioxidative effects of luteolin, kaempferol and pinoresinol were further investigated in H4IIE rat hepatoma cells. A strong protective effect of kaempferol and luteolin was found against H2O2-mediated intracellular reactive oxygen species formation measured using the dichlorofluorescein assay and H2O2-mediated DNA strand breaks. Pinoresinol did not have a protective effect against H2O2-mediated DNA-damage, but in the dichlorofluorescein assay, an antioxidative effect was detectable. During studies with H4IIE cells, kaempferol, luteolin and pinoresinol were taken up by the cells within 60 min. The flavonoids were found to be relatively toxic at higher concentrations, while pinoresinol was less cytotoxic. In conclusion, kaempferol and luteolin, at low concentrations (< or = 50 microM), protect H4IIE cells against oxidative stress but are cytotoxic at higher concentrations; the biological effects of pinoresinol are less prominent in comparison. These results are important for the identification of pharmacologically active substances from traditional Indonesian medicinal plants.     

New sesquiterpene and phenolic glucosides from Saussurea involucrata

One new guaiane-type sesquiterpene glucoside (1) and one new phenolic glucoside (2) were isolated from the whole herb of Saussurea involucrata. Their structures were established by spectroscopic methods, mainly 1D and 2D NMR, and mass spectral analysis.     

New sesquiterpene and phenolic glucosides from Saussurea involucrata

One new guaiane-type sesquiterpene glucoside (1) and one new phenolic glucoside (2) were isolated from the whole herb of Saussurea involucrata. Their structures were established by spectroscopic methods, mainly 1D and 2D NMR, and mass spectral analysis.     

Four new lignans from Schisandra sphenanthera

Three new 7,8-secolignans, schisandlignans A–C (1, 2, and 4), one new dibenzocyclooctadiene lignan, schisandlignan D (5), together with nine known lignans 3′,4′-dimethoxybenzoic acid (3″,4″-dimethoxyphenyl)-2-methyl-3-oxobutyl ester (3), gomisin J (6), rubrisandrin A(1b) (7), interiotherin B (8), schisantherin D (9), ( ? )-machilusin (10), ganschisandrine (11), henricine A (12), and (+)-1-hydroxy pinoresinol (13), were isolated from the rattan of Schisandra sphenanthera. Their structures were determined by analysis of 1D and 2D NMR spectroscopic data.     

Minor compounds from the stem bark of Chinese mangrove associate Catunaregam spinosa

A new dihydroisocoumarin 3-(2-hydroxypropyl)-8-hydroxyl-3,4-dihydroisocoumarin (1) and a new iridoid 3-deoxyartselaenin C (5), together with six known compounds scopoletin (2), scoparone (3), morindolide (4), pinoresinol (6), medioresinol (7), and secoisolariciresinol (8) were isolated from the stem bark of Chinese mangrove associate Catunaregam spinosa Tirveng. The structures of 1 and 5 were determined by extensive spectroscopic analysis, including 1D and 2D NMR data. All compounds except 2 were obtained from C. spinosa for the first time. And it was also the first time lignans were obtained from Catunaregam sp.